Mutagenetix > Incidental Mutation

Incidental Mutation 'R5184:Or14a257'

397753

Institutional Source

Beutler Lab

Gene Symbol

Or14a257

Ensembl Gene

ENSMUSG00000062878

Gene Name

olfactory receptor family 14 subfamily A member 257

Synonyms

GA_x6K02T2NHDJ-9619796-9620794, Olfr298, MOR219-4

MMRRC Submission

042763-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.137) question?

Stock #

R5184 (G1)

Quality Score

217

Status

Validated

Chromosome

Chromosomal Location

86137759-86138757 bp(-) (GRCm39)

Type of Mutation

frame shift

DNA Base Change (assembly)

TG to T at 86138499 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Ref Sequence

ENSEMBL: ENSMUSP00000150228 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000077800] [ENSMUST00000213223] [ENSMUST00000216968] [ENSMUST00000217110]

AlphaFold

Q7TS06

Predicted Effect

probably null
Transcript: ENSMUST00000077800

SMART Domains

Protein: ENSMUSP00000076973
Gene: ENSMUSG00000062878

Domain	Start	End	E-Value	Type
Pfam:7tm_4	29	306	4.2e-37	PFAM
Pfam:7tm_1	39	288	8.4e-19	PFAM

Predicted Effect

probably null
Transcript: ENSMUST00000213223

Predicted Effect

probably null
Transcript: ENSMUST00000216968

Predicted Effect

probably null
Transcript: ENSMUST00000217110

Meta Mutation Damage Score

0.9756

Coding Region Coverage

1x: 99.2%
3x: 98.6%
10x: 97.1%
20x: 94.6%

Validation Efficiency

98% (63/64)

MGI Phenotype

FUNCTION: Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]

Allele List at MGI

Other mutations in this stock

Total: 59 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Aph1a	T	A	3: 95,803,051 (GRCm39)		probably null	Het
Arid3a	A	G	10: 79,786,603 (GRCm39)	E452G	possibly damaging	Het
Barhl2	A	G	5: 106,605,506 (GRCm39)	S68P	unknown	Het
Carf	T	C	1: 60,147,333 (GRCm39)	S17P	probably damaging	Het
Ccdc90b	A	G	7: 92,224,019 (GRCm39)	D145G	probably damaging	Het
Cyp7a1	A	T	4: 6,271,207 (GRCm39)	N316K	probably benign	Het
Daam2	A	G	17: 49,801,419 (GRCm39)	I106T	possibly damaging	Het
Dmtf1	A	G	5: 9,176,641 (GRCm39)	V437A	probably benign	Het
E2f2	A	T	4: 135,911,751 (GRCm39)	Y254F	possibly damaging	Het
Ehmt1	G	T	2: 24,767,509 (GRCm39)	P135T	probably damaging	Het
Ern2	T	C	7: 121,779,182 (GRCm39)	T221A	probably benign	Het
Fam227b	G	A	2: 125,958,043 (GRCm39)	P241S	probably damaging	Het
Fermt1	A	G	2: 132,783,883 (GRCm39)	V42A	possibly damaging	Het
Fgf3	A	G	7: 144,396,547 (GRCm39)	D187G	probably damaging	Het
Flnb	T	A	14: 7,901,945 (GRCm38)	V936E	probably damaging	Het
Herc2	T	C	7: 55,772,099 (GRCm39)	Y1093H	probably damaging	Het
Invs	A	G	4: 48,283,242 (GRCm39)		probably benign	Het
Katnb1	T	A	8: 95,824,608 (GRCm39)	I534N	possibly damaging	Het
Kcnq5	A	T	1: 21,472,711 (GRCm39)	D826E	probably damaging	Het
Klra9	A	T	6: 130,165,675 (GRCm39)	N113K	probably benign	Het
Knl1	A	G	2: 118,899,657 (GRCm39)	K453E	probably damaging	Het
Lgi4	C	T	7: 30,770,182 (GRCm39)		probably benign	Het
Lpin3	A	G	2: 160,739,058 (GRCm39)	S289G	probably benign	Het
Mapre1	T	A	2: 153,599,987 (GRCm39)	V137E	possibly damaging	Het
Mark4	A	G	7: 19,181,168 (GRCm39)	F144L	possibly damaging	Het
Mogat2	G	T	7: 98,872,842 (GRCm39)	A114E	possibly damaging	Het
Msh2	C	A	17: 88,030,841 (GRCm39)	A906E	probably benign	Het
Nell2	A	G	15: 95,425,690 (GRCm39)	L19P	possibly damaging	Het
Nfia	A	T	4: 97,671,585 (GRCm39)	K98N	probably damaging	Het
Nutm1	T	A	2: 112,079,345 (GRCm39)	T857S	possibly damaging	Het
Or52a5	T	C	7: 103,426,611 (GRCm39)	K314E	probably benign	Het
Or8g17	T	A	9: 38,929,998 (GRCm39)	I280F	probably damaging	Het
Pde10a	T	C	17: 9,195,987 (GRCm39)	L706P	probably damaging	Het
Penk	A	G	4: 4,134,296 (GRCm39)	L117P	probably damaging	Het
Pgap1	A	G	1: 54,521,015 (GRCm39)	S876P	probably damaging	Het
Pmfbp1	T	C	8: 110,254,399 (GRCm39)	I478T	possibly damaging	Het
Pole	G	A	5: 110,442,800 (GRCm39)	E318K	possibly damaging	Het
Prr12	C	A	7: 44,695,801 (GRCm39)	V1222L	unknown	Het
Prune2	T	C	19: 17,193,721 (GRCm39)	S3070P	possibly damaging	Het
Rnf19a	A	G	15: 36,244,342 (GRCm39)	C573R	probably benign	Het
Rp1l1	T	C	14: 64,267,629 (GRCm39)	S1072P	probably damaging	Het
Rskr	T	C	11: 78,183,573 (GRCm39)	F194L	probably benign	Het
Semp2l1	A	T	1: 32,584,729 (GRCm39)	C394S	probably damaging	Het
Sh3pxd2a	T	C	19: 47,261,850 (GRCm39)	N315S	possibly damaging	Het
Snph	T	C	2: 151,436,464 (GRCm39)	I86V	probably damaging	Het
Sox6	A	G	7: 115,376,463 (GRCm39)	Y90H	probably damaging	Het
Tbc1d13	T	A	2: 30,032,323 (GRCm39)	C145S	probably benign	Het
Tcp11l1	T	C	2: 104,530,289 (GRCm39)	E65G	probably damaging	Het
Thrb	G	T	14: 18,011,181 (GRCm38)	E186*	probably null	Het
Tmem101	A	G	11: 102,047,059 (GRCm39)	Y38H	possibly damaging	Het
Trarg1	T	A	11: 76,571,134 (GRCm39)	L50Q	probably damaging	Het
Urb1	A	T	16: 90,580,162 (GRCm39)		probably null	Het
Usp10	G	A	8: 120,683,420 (GRCm39)	V764I	possibly damaging	Het
Usp25	A	G	16: 76,906,115 (GRCm39)	E857G	probably benign	Het
Vmn1r17	A	G	6: 57,337,828 (GRCm39)	V130A	probably benign	Het
Wdr35	T	C	12: 9,068,142 (GRCm39)	L775P	probably damaging	Het
Xpnpep1	T	C	19: 53,001,845 (GRCm39)	T134A	probably benign	Het
Zbtb10	T	C	3: 9,329,731 (GRCm39)	V363A	probably damaging	Het
Zfp108	G	T	7: 23,960,163 (GRCm39)	K251N	probably benign	Het

Other mutations in Or14a257

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01517:Or14a257	APN	7	86,138,765 (GRCm39)	utr 5 prime	probably benign
IGL01603:Or14a257	APN	7	86,138,139 (GRCm39)	missense	possibly damaging	0.92
IGL02389:Or14a257	APN	7	86,138,336 (GRCm39)	missense	probably damaging	0.98
IGL03146:Or14a257	APN	7	86,138,402 (GRCm39)	missense	probably damaging	1.00
R0680:Or14a257	UTSW	7	86,138,545 (GRCm39)	missense	probably benign
R0834:Or14a257	UTSW	7	86,138,698 (GRCm39)	missense	probably benign	0.11
R1132:Or14a257	UTSW	7	86,138,425 (GRCm39)	missense	probably benign	0.03
R1519:Or14a257	UTSW	7	86,138,333 (GRCm39)	missense	probably damaging	0.99
R1881:Or14a257	UTSW	7	86,138,646 (GRCm39)	missense	probably benign	0.01
R2170:Or14a257	UTSW	7	86,137,778 (GRCm39)	missense	probably benign	0.03
R4500:Or14a257	UTSW	7	86,138,127 (GRCm39)	missense	probably benign	0.00
R4524:Or14a257	UTSW	7	86,138,034 (GRCm39)	missense	probably damaging	1.00
R5521:Or14a257	UTSW	7	86,137,839 (GRCm39)	missense	probably benign	0.08
R7448:Or14a257	UTSW	7	86,138,417 (GRCm39)	missense	probably damaging	1.00
R7643:Or14a257	UTSW	7	86,138,776 (GRCm39)	critical splice acceptor site	probably null
R7688:Or14a257	UTSW	7	86,138,183 (GRCm39)	missense	probably benign	0.01
R8104:Or14a257	UTSW	7	86,138,231 (GRCm39)	missense	probably damaging	1.00
R8732:Or14a257	UTSW	7	86,138,061 (GRCm39)	missense	probably damaging	0.96
R8973:Or14a257	UTSW	7	86,138,487 (GRCm39)	missense	probably damaging	1.00
R9235:Or14a257	UTSW	7	86,138,306 (GRCm39)	missense	probably benign	0.01

Predicted Primers

PCR Primer
(F):5'- TGCCAGCTGTGTATAAAGCTC -3'
(R):5'- ACAGACTGTATGTGGGCTGTTC -3'

Sequencing Primer
(F):5'- GCCAGCTGTGTATAAAGCTCCAAAG -3'
(R):5'- CTTTCTGGTAATGTACGTTGCAGTC -3'

Posted On

2016-07-06