Mutagenetix > Incidental Mutation

Incidental Mutation 'R5233:Or8b38'

398137

Institutional Source

Beutler Lab

Gene Symbol

Or8b38

Ensembl Gene

ENSMUSG00000096424

Gene Name

olfactory receptor family 8 subfamily B member 38

Synonyms

MOR162-12, GA_x6K02T2PVTD-31740639-31741568, Olfr885

MMRRC Submission

042805-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.086) question?

Stock #

R5233 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

37972618-37973547 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to G at 37973446 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Threonine to Alanine at position 277 (T277A)

Ref Sequence

ENSEMBL: ENSMUSP00000080646 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000081985]

AlphaFold

Q7TRE0

Predicted Effect

probably damaging
Transcript: ENSMUST00000081985
AA Change: T277A

PolyPhen 2 Score 0.963 (Sensitivity: 0.78; Specificity: 0.95)

SMART Domains

Protein: ENSMUSP00000080646
Gene: ENSMUSG00000096424
AA Change: T277A

Domain	Start	End	E-Value	Type
Pfam:7tm_4	31	306	3.9e-51	PFAM
Pfam:7TM_GPCR_Srsx	35	224	1.1e-5	PFAM
Pfam:7tm_1	41	288	7.5e-24	PFAM

Meta Mutation Damage Score

0.4193

Coding Region Coverage

1x: 99.2%
3x: 98.5%
10x: 97.0%
20x: 94.9%

Validation Efficiency

100% (56/56)

MGI Phenotype

FUNCTION: Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]

Allele List at MGI

Other mutations in this stock

Total: 51 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
9030619P08Rik	T	A	15: 75,301,801 (GRCm39)		noncoding transcript	Het
Aadat	A	G	8: 60,979,656 (GRCm39)	I173V	probably benign	Het
Acsl6	G	A	11: 54,216,432 (GRCm39)	V200I	possibly damaging	Het
Alms1	T	A	6: 85,633,353 (GRCm39)		probably null	Het
Arhgef17	A	T	7: 100,530,576 (GRCm39)	D1403E	possibly damaging	Het
Atp10b	A	G	11: 43,121,387 (GRCm39)	T1017A	probably benign	Het
Capg	C	T	6: 72,532,509 (GRCm39)	R25C	probably damaging	Het
Cd22	A	G	7: 30,576,959 (GRCm39)	I116T	probably damaging	Het
Cep135	AGTCTGCCTTTGG	A	5: 76,739,690 (GRCm39)		probably benign	Het
Ciita	A	T	16: 10,327,265 (GRCm39)	I277F	possibly damaging	Het
Col15a1	T	C	4: 47,296,112 (GRCm39)	V943A	possibly damaging	Het
Coq2	G	A	5: 100,805,698 (GRCm39)	H313Y	possibly damaging	Het
Csn3	C	T	5: 88,077,694 (GRCm39)	P67S	probably benign	Het
Csrnp3	A	G	2: 65,852,684 (GRCm39)	T359A	possibly damaging	Het
Cttnbp2nl	A	G	3: 104,912,357 (GRCm39)	L509P	probably damaging	Het
Dclk3	A	G	9: 111,297,749 (GRCm39)	D431G	probably benign	Het
Dtx3l	A	T	16: 35,753,608 (GRCm39)	C333S	possibly damaging	Het
Ear-ps2	G	A	14: 44,284,517 (GRCm39)		noncoding transcript	Het
Fam171a1	G	C	2: 3,179,390 (GRCm39)	G72A	probably damaging	Het
Fbxw17	T	C	13: 50,586,390 (GRCm39)		probably benign	Het
Fry	A	G	5: 150,393,185 (GRCm39)	D605G	possibly damaging	Het
Fyn	T	C	10: 39,405,936 (GRCm39)	F240S	probably benign	Het
Gcfc2	T	C	6: 81,930,271 (GRCm39)	L646P	probably damaging	Het
Gm5087	C	T	14: 13,158,788 (GRCm38)		noncoding transcript	Het
Got2	T	A	8: 96,602,477 (GRCm39)	N91I	probably benign	Het
Hspa4	C	T	11: 53,177,802 (GRCm39)	V103I	possibly damaging	Het
Itgad	A	G	7: 127,792,600 (GRCm39)		probably null	Het
Krt33a	A	G	11: 99,904,961 (GRCm39)	S182P	probably damaging	Het
Mcph1	T	C	8: 18,721,254 (GRCm39)	I694T	probably damaging	Het
Mmp15	T	C	8: 96,097,696 (GRCm39)	V502A	probably benign	Het
Mov10l1	T	C	15: 88,867,235 (GRCm39)	V23A	probably benign	Het
Myo9a	T	A	9: 59,817,900 (GRCm39)	N2322K	probably damaging	Het
Ndst4	A	G	3: 125,503,766 (GRCm39)	Y11C	probably damaging	Het
Nell1	T	C	7: 49,826,062 (GRCm39)	F199S	probably damaging	Het
Nup210	C	T	6: 91,003,951 (GRCm39)	V646M	probably damaging	Het
Nup98	A	T	7: 101,845,029 (GRCm39)	S13R	unknown	Het
Nxf1	T	C	19: 8,741,293 (GRCm39)	I54T	possibly damaging	Het
Pcdh10	C	A	3: 45,338,626 (GRCm39)	R928S	probably damaging	Het
Pou3f3	A	G	1: 42,737,438 (GRCm39)	N378S	probably benign	Het
Rfx6	T	A	10: 51,588,187 (GRCm39)	Y109*	probably null	Het
Rorc	T	A	3: 94,304,632 (GRCm39)	V339D	probably benign	Het
Scin	T	A	12: 40,127,558 (GRCm39)	I411F	probably benign	Het
Serpind1	A	G	16: 17,154,758 (GRCm39)	Y195C	probably damaging	Het
Tas2r117	T	C	6: 132,780,585 (GRCm39)	V241A	possibly damaging	Het
Tet3	T	A	6: 83,363,045 (GRCm39)	E709V	probably damaging	Het
Trbc1	T	A	6: 41,515,383 (GRCm39)		probably benign	Het
Vmn1r212	C	A	13: 23,067,304 (GRCm39)	G343V	unknown	Het
Vmn2r42	T	A	7: 8,197,837 (GRCm39)	K261*	probably null	Het
Xrcc5	T	A	1: 72,379,209 (GRCm39)	L438Q	probably damaging	Het
Zfp142	T	C	1: 74,624,608 (GRCm39)	N72S	probably damaging	Het
Zfp292	A	T	4: 34,809,755 (GRCm39)	S1096R	probably damaging	Het

Other mutations in Or8b38

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01470:Or8b38	APN	9	37,973,300 (GRCm39)	missense	possibly damaging	0.58
IGL02441:Or8b38	APN	9	37,973,233 (GRCm39)	missense	probably benign	0.02
IGL03263:Or8b38	APN	9	37,973,009 (GRCm39)	missense	probably damaging	0.98
R1748:Or8b38	UTSW	9	37,972,796 (GRCm39)	missense	probably damaging	1.00
R1870:Or8b38	UTSW	9	37,972,646 (GRCm39)	missense	probably benign	0.24
R1920:Or8b38	UTSW	9	37,972,981 (GRCm39)	missense	probably damaging	1.00
R1921:Or8b38	UTSW	9	37,972,981 (GRCm39)	missense	probably damaging	1.00
R1922:Or8b38	UTSW	9	37,972,981 (GRCm39)	missense	probably damaging	1.00
R4073:Or8b38	UTSW	9	37,973,165 (GRCm39)	missense	probably damaging	1.00
R4727:Or8b38	UTSW	9	37,973,389 (GRCm39)	missense	probably damaging	1.00
R4945:Or8b38	UTSW	9	37,973,068 (GRCm39)	missense	probably damaging	1.00
R4950:Or8b38	UTSW	9	37,973,297 (GRCm39)	missense	probably damaging	1.00
R5116:Or8b38	UTSW	9	37,972,634 (GRCm39)	missense	probably benign
R5923:Or8b38	UTSW	9	37,973,154 (GRCm39)	missense	probably benign	0.08
R6668:Or8b38	UTSW	9	37,973,066 (GRCm39)	missense	possibly damaging	0.83
R8035:Or8b38	UTSW	9	37,972,961 (GRCm39)	missense	probably damaging	0.99
R9392:Or8b38	UTSW	9	37,973,195 (GRCm39)	missense	probably benign	0.01
R9481:Or8b38	UTSW	9	37,972,707 (GRCm39)	missense	probably benign	0.01
RF020:Or8b38	UTSW	9	37,972,620 (GRCm39)	start codon destroyed	probably null	0.01

Predicted Primers

PCR Primer
(F):5'- TATAACAGTGCCCAGCCTGAC -3'
(R):5'- TAATTCTAGATTCCCACCTCAAGG -3'

Sequencing Primer
(F):5'- GCCCAGCCTGACTCTCTTTG -3'
(R):5'- CTGTAGAGCAAATCTAGGGCCTC -3'

Posted On

2016-07-06