Mutagenetix > Incidental Mutation

Incidental Mutation 'R5238:Vmn1r158'

400500

Institutional Source

Beutler Lab

Gene Symbol

Vmn1r158

Ensembl Gene

ENSMUSG00000094700

Gene Name

vomeronasal 1 receptor 158

Synonyms

Gm16455

MMRRC Submission

042809-MU

Accession Numbers

Essential gene?

Possibly essential (E-score: 0.653) question?

Stock #

R5238 (G1)

Quality Score

195

Status

Not validated

Chromosome

Chromosomal Location

22489284-22490207 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to A at 22489799 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Methionine to Leucine at position 137 (M137L)

Ref Sequence

ENSEMBL: ENSMUSP00000133990 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000174643]

AlphaFold

G3UY92

Predicted Effect

probably benign
Transcript: ENSMUST00000174643
AA Change: M137L

PolyPhen 2 Score 0.000 (Sensitivity: 1.00; Specificity: 0.00)

SMART Domains

Protein: ENSMUSP00000133990
Gene: ENSMUSG00000094700
AA Change: M137L

Domain	Start	End	E-Value	Type
Pfam:TAS2R	8	298	2.9e-15	PFAM
Pfam:7tm_1	39	290	5.5e-9	PFAM
Pfam:V1R	41	298	3.2e-17	PFAM

Coding Region Coverage

1x: 99.2%
3x: 98.5%
10x: 97.0%
20x: 94.7%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 39 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Abhd12b	T	A	12: 70,210,142 (GRCm39)		probably null	Het
Adamtsl5	C	T	10: 80,181,192 (GRCm39)	G63D	probably damaging	Het
Armc9	T	A	1: 86,127,569 (GRCm39)	M68K	probably benign	Het
Atad2	A	C	15: 57,971,733 (GRCm39)	H381Q	possibly damaging	Het
Bclaf1	T	G	10: 20,208,130 (GRCm39)		probably benign	Het
Ccdc188	A	G	16: 18,037,038 (GRCm39)	E238G	probably damaging	Het
Cldn19	G	T	4: 119,112,930 (GRCm39)	C54F	probably damaging	Het
Clip1	T	C	5: 123,785,946 (GRCm39)	D246G	probably damaging	Het
Col20a1	T	C	2: 180,640,379 (GRCm39)	V512A	probably damaging	Het
Cyfip1	G	T	7: 55,541,779 (GRCm39)	A355S	probably benign	Het
Dffa	T	G	4: 149,188,760 (GRCm39)	L18R	probably benign	Het
Dnah8	G	A	17: 31,009,891 (GRCm39)	E3761K	probably damaging	Het
Dusp10	A	G	1: 183,769,210 (GRCm39)	T59A	possibly damaging	Het
Eed	T	C	7: 89,626,173 (GRCm39)	S67G	probably benign	Het
Fam181a	C	T	12: 103,282,392 (GRCm39)	A99V	probably benign	Het
Gm12185	G	A	11: 48,799,044 (GRCm39)	T483I	possibly damaging	Het
Htr3b	A	T	9: 48,848,542 (GRCm39)	C234*	probably null	Het
Kidins220	C	A	12: 25,053,009 (GRCm39)	T433K	probably benign	Het
Man2a1	T	C	17: 64,943,502 (GRCm39)	Y186H	probably damaging	Het
Mcm9	G	T	10: 53,506,093 (GRCm39)	S60R	possibly damaging	Het
Mst1r	T	A	9: 107,784,773 (GRCm39)	C144S	probably damaging	Het
Nckap5	A	G	1: 125,955,461 (GRCm39)	C364R	probably damaging	Het
Nptx2	T	C	5: 144,493,041 (GRCm39)	I376T	probably damaging	Het
Or2a14	T	C	6: 43,130,961 (GRCm39)	S241P	probably damaging	Het
Otogl	A	T	10: 107,604,834 (GRCm39)	C2191S	probably damaging	Het
Plxdc2	T	A	2: 16,655,026 (GRCm39)	F208L	probably damaging	Het
Robo3	A	C	9: 37,328,175 (GRCm39)	Y1339D	probably damaging	Het
Rsph9	G	T	17: 46,446,008 (GRCm39)	Y42*	probably null	Het
Slc39a1	T	A	3: 90,156,702 (GRCm39)	L86Q	probably null	Het
Slfn8	T	C	11: 82,904,214 (GRCm39)	D392G	probably damaging	Het
Tiprl	A	G	1: 165,043,337 (GRCm39)	V263A	probably benign	Het
Tmub2	A	G	11: 102,175,820 (GRCm39)		probably benign	Het
Trpm1	T	A	7: 63,918,702 (GRCm39)	F681I	probably damaging	Het
Ttn	A	T	2: 76,641,587 (GRCm39)	L5176Q	possibly damaging	Het
Uba3	A	T	6: 97,178,896 (GRCm39)	C68*	probably null	Het
Vmn1r50	C	T	6: 90,084,465 (GRCm39)	A70V	possibly damaging	Het
Wwc1	T	C	11: 35,766,723 (GRCm39)	K511E	probably benign	Het
Zfp600	T	C	4: 146,131,741 (GRCm39)		probably null	Het
Zng1	G	T	19: 24,897,994 (GRCm39)	T382K	probably damaging	Het

Other mutations in Vmn1r158

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01147:Vmn1r158	APN	7	22,490,204 (GRCm39)	missense	probably benign	0.01
R1173:Vmn1r158	UTSW	7	22,489,870 (GRCm39)	missense	probably benign	0.19
R1574:Vmn1r158	UTSW	7	22,489,772 (GRCm39)	missense	probably damaging	1.00
R1574:Vmn1r158	UTSW	7	22,489,772 (GRCm39)	missense	probably damaging	1.00
R1725:Vmn1r158	UTSW	7	22,490,072 (GRCm39)	missense	probably benign	0.08
R1777:Vmn1r158	UTSW	7	22,489,855 (GRCm39)	missense	probably damaging	1.00
R1813:Vmn1r158	UTSW	7	22,490,143 (GRCm39)	missense	probably damaging	1.00
R1896:Vmn1r158	UTSW	7	22,490,143 (GRCm39)	missense	probably damaging	1.00
R2077:Vmn1r158	UTSW	7	22,489,815 (GRCm39)	missense	probably benign	0.03
R3749:Vmn1r158	UTSW	7	22,489,639 (GRCm39)	missense	probably damaging	1.00
R4872:Vmn1r158	UTSW	7	22,490,179 (GRCm39)	missense	possibly damaging	0.94
R6500:Vmn1r158	UTSW	7	22,490,078 (GRCm39)	missense	possibly damaging	0.89
R6511:Vmn1r158	UTSW	7	22,490,116 (GRCm39)	missense	probably benign	0.00
R6581:Vmn1r158	UTSW	7	22,489,465 (GRCm39)	missense	possibly damaging	0.69
R6751:Vmn1r158	UTSW	7	22,489,306 (GRCm39)	missense	probably damaging	0.96
R7168:Vmn1r158	UTSW	7	22,490,101 (GRCm39)	missense	possibly damaging	0.82
R7337:Vmn1r158	UTSW	7	22,489,649 (GRCm39)	missense	probably benign	0.00
R7747:Vmn1r158	UTSW	7	22,489,725 (GRCm39)	missense	probably benign	0.15
R7902:Vmn1r158	UTSW	7	22,489,433 (GRCm39)	missense	possibly damaging	0.67
R8328:Vmn1r158	UTSW	7	22,489,487 (GRCm39)	missense	probably damaging	1.00
R8400:Vmn1r158	UTSW	7	22,489,305 (GRCm39)	nonsense	probably null
R8809:Vmn1r158	UTSW	7	22,489,775 (GRCm39)	missense	probably damaging	0.97
R9227:Vmn1r158	UTSW	7	22,489,469 (GRCm39)	missense	probably benign	0.02
R9468:Vmn1r158	UTSW	7	22,489,888 (GRCm39)	missense	probably damaging	1.00
R9702:Vmn1r158	UTSW	7	22,490,065 (GRCm39)	missense	probably benign	0.00
R9719:Vmn1r158	UTSW	7	22,489,331 (GRCm39)	missense	possibly damaging	0.95
Z1177:Vmn1r158	UTSW	7	22,489,883 (GRCm39)	missense	possibly damaging	0.46

Predicted Primers

PCR Primer
(F):5'- TGGCATCATAGGCAAACTGC -3'
(R):5'- ACAGGTGATTTTAAGCCACATGG -3'

Sequencing Primer
(F):5'- CTGCAAGAGGACAGTGCCTAC -3'
(R):5'- CCAAACAACATGATGGCTTTTGCTC -3'

Posted On

2016-07-06