Mutagenetix > Incidental Mutation

Incidental Mutation 'R5246:Vmn2r45'

401152

Institutional Source

Beutler Lab

Gene Symbol

Vmn2r45

Ensembl Gene

ENSMUSG00000090662

Gene Name

vomeronasal 2, receptor 45

Synonyms

MMRRC Submission

042817-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.073) question?

Stock #

R5246 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

8474468-8491958 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to G at 8486251 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Threonine to Proline at position 346 (T346P)

Ref Sequence

ENSEMBL: ENSMUSP00000129466 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000164845]

AlphaFold

L7N2B5

Predicted Effect

probably benign
Transcript: ENSMUST00000164845
AA Change: T346P

PolyPhen 2 Score 0.001 (Sensitivity: 0.99; Specificity: 0.15)

SMART Domains

Protein: ENSMUSP00000129466
Gene: ENSMUSG00000090662
AA Change: T346P

Domain	Start	End	E-Value	Type
signal peptide	1	18	N/A	INTRINSIC
Pfam:ANF_receptor	154	469	4.5e-26	PFAM
Pfam:NCD3G	512	565	6.4e-21	PFAM
Pfam:7tm_3	598	833	2.1e-54	PFAM

Coding Region Coverage

1x: 99.1%
3x: 98.3%
10x: 96.3%
20x: 92.3%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 30 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Adgrg6	T	A	10: 14,302,509 (GRCm39)	Y968F	probably damaging	Het
Bltp1	G	A	3: 37,102,199 (GRCm39)	R1299Q	probably damaging	Het
Brwd1	G	A	16: 95,803,757 (GRCm39)	P2138S	probably damaging	Het
Cdkl4	T	A	17: 80,846,913 (GRCm39)		probably null	Het
Cep55	T	A	19: 38,058,119 (GRCm39)	D237E	probably benign	Het
Clhc1	T	A	11: 29,525,434 (GRCm39)	S503T	probably damaging	Het
Cybb	C	G	X: 9,316,989 (GRCm39)	D246H	probably benign	Het
Efcab5	T	C	11: 77,079,671 (GRCm39)	Y25C	probably damaging	Het
Eif3c	G	A	7: 126,156,410 (GRCm39)	T444I	possibly damaging	Het
Epb41l4b	G	T	4: 57,040,989 (GRCm39)	N491K	probably damaging	Het
Gm9776	A	T	13: 94,495,065 (GRCm39)		probably benign	Het
Lrcol1	A	C	5: 110,502,377 (GRCm39)	E84D	possibly damaging	Het
Lrp1b	A	G	2: 41,360,952 (GRCm39)		probably null	Het
Mup5	T	A	4: 61,752,874 (GRCm39)	I50L	probably benign	Het
Myo16	A	T	8: 10,612,212 (GRCm39)	K1286*	probably null	Het
Pramel22	G	T	4: 143,382,127 (GRCm39)	P190T	probably benign	Het
Prss46	T	A	9: 110,679,102 (GRCm39)	V101D	probably damaging	Het
Rint1	T	A	5: 24,005,809 (GRCm39)	W164R	probably damaging	Het
Rnf139	G	A	15: 58,771,552 (GRCm39)	V526I	probably damaging	Het
Rptn	A	T	3: 93,304,140 (GRCm39)	D491V	probably damaging	Het
Rptn	A	G	3: 93,305,036 (GRCm39)	T790A	possibly damaging	Het
Scn8a	T	C	15: 100,908,938 (GRCm39)	L802P	probably damaging	Het
Scrib	T	C	15: 75,936,646 (GRCm39)	E435G	probably benign	Het
Sdk1	C	A	5: 142,100,317 (GRCm39)	T1449K	possibly damaging	Het
Septin7	T	A	9: 25,210,832 (GRCm39)	L289M	probably damaging	Het
Spry1	A	G	3: 37,696,916 (GRCm39)	Y53C	probably damaging	Het
Srpk3	C	T	X: 72,818,555 (GRCm39)	R82*	probably null	Het
Tmcc1	A	G	6: 116,020,381 (GRCm39)	V142A	probably damaging	Het
Wdr25	A	G	12: 108,993,382 (GRCm39)	I533V	probably benign	Het
Zfp292	A	C	4: 34,805,842 (GRCm39)	Y2401D	possibly damaging	Het

Other mutations in Vmn2r45

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01287:Vmn2r45	APN	7	8,488,622 (GRCm39)	missense	probably benign	0.00
IGL01322:Vmn2r45	APN	7	8,484,332 (GRCm39)	missense	possibly damaging	0.49
IGL01402:Vmn2r45	APN	7	8,484,467 (GRCm39)	missense	probably damaging	0.98
IGL01404:Vmn2r45	APN	7	8,484,467 (GRCm39)	missense	probably damaging	0.98
IGL01529:Vmn2r45	APN	7	8,486,493 (GRCm39)	missense	probably benign
IGL01596:Vmn2r45	APN	7	8,486,272 (GRCm39)	missense	probably damaging	0.98
IGL01646:Vmn2r45	APN	7	8,486,337 (GRCm39)	missense	probably benign	0.18
IGL01819:Vmn2r45	APN	7	8,488,556 (GRCm39)	missense	probably benign	0.00
IGL02096:Vmn2r45	APN	7	8,484,443 (GRCm39)	missense	probably damaging	0.99
IGL02130:Vmn2r45	APN	7	8,486,556 (GRCm39)	missense	possibly damaging	0.46
IGL02409:Vmn2r45	APN	7	8,488,727 (GRCm39)	missense	probably benign	0.03
IGL02517:Vmn2r45	APN	7	8,486,185 (GRCm39)	nonsense	probably null
IGL02633:Vmn2r45	APN	7	8,488,728 (GRCm39)	missense	probably benign	0.01
IGL02940:Vmn2r45	APN	7	8,475,369 (GRCm39)	missense	probably damaging	0.99
IGL03281:Vmn2r45	APN	7	8,486,603 (GRCm39)	missense	probably damaging	1.00
IGL03358:Vmn2r45	APN	7	8,474,715 (GRCm39)	missense	probably damaging	1.00
BB004:Vmn2r45	UTSW	7	8,486,513 (GRCm39)	missense	probably benign
BB014:Vmn2r45	UTSW	7	8,486,513 (GRCm39)	missense	probably benign
R0382:Vmn2r45	UTSW	7	8,486,098 (GRCm39)	missense	probably benign	0.06
R0532:Vmn2r45	UTSW	7	8,474,820 (GRCm39)	missense	probably damaging	0.99
R0932:Vmn2r45	UTSW	7	8,478,380 (GRCm39)	missense	probably damaging	1.00
R1125:Vmn2r45	UTSW	7	8,488,542 (GRCm39)	missense	probably benign	0.00
R1580:Vmn2r45	UTSW	7	8,474,746 (GRCm39)	missense	possibly damaging	0.95
R1817:Vmn2r45	UTSW	7	8,475,372 (GRCm39)	missense	probably damaging	1.00
R1996:Vmn2r45	UTSW	7	8,475,024 (GRCm39)	missense	probably damaging	1.00
R2050:Vmn2r45	UTSW	7	8,475,021 (GRCm39)	missense	probably damaging	1.00
R2284:Vmn2r45	UTSW	7	8,488,765 (GRCm39)	missense	probably benign	0.02
R4019:Vmn2r45	UTSW	7	8,474,580 (GRCm39)	nonsense	probably null
R4227:Vmn2r45	UTSW	7	8,486,277 (GRCm39)	missense	probably damaging	0.98
R4381:Vmn2r45	UTSW	7	8,474,912 (GRCm39)	nonsense	probably null
R4618:Vmn2r45	UTSW	7	8,486,436 (GRCm39)	missense	probably benign	0.00
R4624:Vmn2r45	UTSW	7	8,484,341 (GRCm39)	missense	probably damaging	1.00
R4704:Vmn2r45	UTSW	7	8,486,535 (GRCm39)	nonsense	probably null
R4735:Vmn2r45	UTSW	7	8,486,472 (GRCm39)	missense	probably damaging	1.00
R4868:Vmn2r45	UTSW	7	8,484,480 (GRCm39)	missense	probably benign	0.06
R4983:Vmn2r45	UTSW	7	8,486,116 (GRCm39)	missense	probably damaging	0.99
R5430:Vmn2r45	UTSW	7	8,486,333 (GRCm39)	nonsense	probably null
R5504:Vmn2r45	UTSW	7	8,486,176 (GRCm39)	missense	probably benign	0.03
R5511:Vmn2r45	UTSW	7	8,474,832 (GRCm39)	missense	probably benign	0.19
R5745:Vmn2r45	UTSW	7	8,486,074 (GRCm39)	missense	probably benign	0.00
R5814:Vmn2r45	UTSW	7	8,474,475 (GRCm39)	missense	probably benign	0.00
R6223:Vmn2r45	UTSW	7	8,486,301 (GRCm39)	missense	probably benign
R6267:Vmn2r45	UTSW	7	8,475,207 (GRCm39)	missense	probably benign	0.26
R6623:Vmn2r45	UTSW	7	8,474,500 (GRCm39)	missense	probably benign	0.09
R6999:Vmn2r45	UTSW	7	8,486,219 (GRCm39)	missense	probably benign
R7242:Vmn2r45	UTSW	7	8,488,612 (GRCm39)	nonsense	probably null
R7491:Vmn2r45	UTSW	7	8,484,342 (GRCm39)	missense	probably benign	0.27
R7620:Vmn2r45	UTSW	7	8,486,222 (GRCm39)	nonsense	probably null
R7719:Vmn2r45	UTSW	7	8,486,460 (GRCm39)	missense	probably damaging	0.99
R7720:Vmn2r45	UTSW	7	8,486,460 (GRCm39)	missense	probably damaging	0.99
R7853:Vmn2r45	UTSW	7	8,485,987 (GRCm39)	missense	possibly damaging	0.90
R7873:Vmn2r45	UTSW	7	8,486,074 (GRCm39)	missense	probably benign	0.00
R7882:Vmn2r45	UTSW	7	8,486,409 (GRCm39)	missense	possibly damaging	0.82
R7927:Vmn2r45	UTSW	7	8,486,513 (GRCm39)	missense	probably benign
R8684:Vmn2r45	UTSW	7	8,486,511 (GRCm39)	missense	probably damaging	0.99
R8780:Vmn2r45	UTSW	7	8,484,371 (GRCm39)	missense	possibly damaging	0.94
R8811:Vmn2r45	UTSW	7	8,474,881 (GRCm39)	missense	probably damaging	1.00
R8893:Vmn2r45	UTSW	7	8,488,619 (GRCm39)	missense	probably damaging	1.00
R9043:Vmn2r45	UTSW	7	8,474,904 (GRCm39)	missense	possibly damaging	0.92
R9208:Vmn2r45	UTSW	7	8,486,298 (GRCm39)	missense	probably damaging	1.00
R9327:Vmn2r45	UTSW	7	8,474,532 (GRCm39)	missense	probably damaging	1.00
R9586:Vmn2r45	UTSW	7	8,486,050 (GRCm39)	missense	probably benign	0.01
R9599:Vmn2r45	UTSW	7	8,478,457 (GRCm39)	critical splice acceptor site	probably null
U24488:Vmn2r45	UTSW	7	8,475,361 (GRCm39)	missense	probably damaging	1.00
Z1088:Vmn2r45	UTSW	7	8,474,484 (GRCm39)	missense	probably benign	0.16

Predicted Primers

PCR Primer
(F):5'- TTGCAGATTCATCTCATGGAGGG -3'
(R):5'- GATTTGATCTTCAGAATGTGGGAAC -3'

Sequencing Primer
(F):5'- ATGGGCTATGGCATGCAC -3'
(R):5'- ACCTCCCATTTTACAGAGAATATGG -3'

Posted On

2016-07-06