Mutagenetix > Incidental Mutation

Incidental Mutation 'R5263:Rhob'

401563

Institutional Source

Beutler Lab

Gene Symbol

Rhob

Ensembl Gene

ENSMUSG00000054364

Gene Name

ras homolog family member B

Synonyms

Arhb

MMRRC Submission

042831-MU

Accession Numbers

Essential gene?

Possibly essential (E-score: 0.508) question?

Stock #

R5263 (G1)

Quality Score

207

Status

Not validated

Chromosome

Chromosomal Location

8547661-8550009 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to T at 8549232 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Methionine to Lysine at position 134 (M134K)

Ref Sequence

ENSEMBL: ENSMUSP00000067013 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000067384]

AlphaFold

P62746

Predicted Effect

probably benign
Transcript: ENSMUST00000067384
AA Change: M134K

PolyPhen 2 Score 0.000 (Sensitivity: 1.00; Specificity: 0.00)

SMART Domains

Protein: ENSMUSP00000067013
Gene: ENSMUSG00000054364
AA Change: M134K

Domain	Start	End	E-Value	Type
RHO	8	181	1.13e-127	SMART

Coding Region Coverage

1x: 99.2%
3x: 98.5%
10x: 96.9%
20x: 94.4%

Validation Efficiency

MGI Phenotype

PHENOTYPE: Homozygous mutation of this gene results in increased incidence of DMBA-induced skin tumors. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 35 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Adh4	G	T	3: 138,133,816 (GRCm39)	V309L	probably benign	Het
Agmo	T	C	12: 37,407,680 (GRCm39)	V188A	probably benign	Het
Aqr	A	G	2: 113,947,059 (GRCm39)	M1041T	probably damaging	Het
Arhgef4	T	A	1: 34,764,078 (GRCm39)	S1111R	possibly damaging	Het
Ascc3	A	C	10: 50,592,757 (GRCm39)	E1144D	probably benign	Het
Cct3	T	C	3: 88,228,672 (GRCm39)		probably null	Het
Cd209f	T	C	8: 4,154,506 (GRCm39)	T114A	probably benign	Het
Cgnl1	G	A	9: 71,539,936 (GRCm39)	Q1103*	probably null	Het
Cop1	G	A	1: 159,152,507 (GRCm39)	D586N	probably damaging	Het
Dcaf5	A	G	12: 80,395,120 (GRCm39)	S350P	probably damaging	Het
Dhx29	T	C	13: 113,084,755 (GRCm39)	C658R	probably damaging	Het
Dync1i1	G	A	6: 5,969,446 (GRCm39)	V424I	possibly damaging	Het
Gfap	C	T	11: 102,787,756 (GRCm39)	R63Q	probably damaging	Het
Gm10563	CTTT	CTTTATTT	4: 155,698,940 (GRCm39)		probably null	Het
Gprc6a	C	A	10: 51,502,900 (GRCm39)	G321V	probably damaging	Het
Itgb4	C	T	11: 115,874,983 (GRCm39)	R447W	probably benign	Het
Izumo1r	A	G	9: 14,812,976 (GRCm39)	C99R	probably damaging	Het
Lrp1b	T	C	2: 41,850,691 (GRCm39)	D102G	probably damaging	Het
Mettl4	G	A	17: 95,047,937 (GRCm39)	Q235*	probably null	Het
Mmrn2	A	G	14: 34,121,541 (GRCm39)	T804A	probably benign	Het
Mrgprb5	A	G	7: 47,817,937 (GRCm39)	V266A	probably damaging	Het
Ntng1	T	C	3: 109,842,188 (GRCm39)	D195G	probably damaging	Het
Pld4	T	C	12: 112,731,465 (GRCm39)	L206P	probably damaging	Het
Polr3a	T	C	14: 24,505,009 (GRCm39)	I1084V	possibly damaging	Het
Prkd1	A	G	12: 50,435,089 (GRCm39)	L546P	probably damaging	Het
Ryr3	A	G	2: 112,548,347 (GRCm39)	S3087P	possibly damaging	Het
Sema6c	T	C	3: 95,080,463 (GRCm39)	L887P	probably benign	Het
Sltm	T	C	9: 70,492,081 (GRCm39)	S648P	unknown	Het
Sucnr1	A	G	3: 59,994,190 (GRCm39)	I239M	possibly damaging	Het
Tgtp2	T	A	11: 48,950,090 (GRCm39)	M161L	probably damaging	Het
Trpm7	A	G	2: 126,663,137 (GRCm39)	V1037A	probably benign	Het
Vmn2r13	A	T	5: 109,321,841 (GRCm39)	H285Q	probably benign	Het
Zfp971	T	A	2: 177,675,555 (GRCm39)	C385S	probably damaging	Het
Zfpm2	T	A	15: 40,962,791 (GRCm39)	V283E	probably benign	Het
Zranb1	CTGATGATGATG	CTGATGATGATGATG	7: 132,584,556 (GRCm39)		probably benign	Het

Other mutations in Rhob

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
R0084:Rhob	UTSW	12	8,549,107 (GRCm39)	missense	probably benign	0.39
R1442:Rhob	UTSW	12	8,549,325 (GRCm39)	missense	possibly damaging	0.76
R1484:Rhob	UTSW	12	8,549,388 (GRCm39)	missense	probably damaging	0.97
R2071:Rhob	UTSW	12	8,549,232 (GRCm39)	missense	probably benign	0.00
R4433:Rhob	UTSW	12	8,549,533 (GRCm39)	missense	possibly damaging	0.73
R4569:Rhob	UTSW	12	8,549,373 (GRCm39)	missense	probably damaging	0.97
R6478:Rhob	UTSW	12	8,549,585 (GRCm39)	nonsense	probably null
R8492:Rhob	UTSW	12	8,549,531 (GRCm39)	nonsense	probably null
R9144:Rhob	UTSW	12	8,549,124 (GRCm39)	missense	probably damaging	0.99
R9157:Rhob	UTSW	12	8,549,319 (GRCm39)	missense	probably benign	0.01
Z1176:Rhob	UTSW	12	8,549,326 (GRCm39)	missense	probably benign	0.18

Predicted Primers

PCR Primer
(F):5'- TGATGCAGCCATTCTGGGATC -3'
(R):5'- GAACTATGTGGCGGACATCGAG -3'

Sequencing Primer
(F):5'- AGCCATTCTGGGATCCGTAG -3'
(R):5'- CCAGGAGGACTACGATCGTTTAC -3'

Posted On

2016-07-06