Incidental Mutation 'R5077:Dlg4'
| ID |
401946 |
| Institutional Source |
Beutler Lab
|
| Gene Symbol |
Dlg4
|
| Ensembl Gene |
ENSMUSG00000020886 |
| Gene Name |
discs large MAGUK scaffold protein 4 |
| Synonyms |
SAP90, PSD-95, Dlgh4, SAP90A, PSD95 |
| MMRRC Submission |
042666-MU
|
| Accession Numbers |
|
| Essential gene? |
Non essential
(E-score: 0.000)
|
| Stock # |
R5077 (G1)
|
| Quality Score |
206 |
|
Status
|
Validated
|
| Chromosome |
11 |
| Chromosomal Location |
69908029-69938107 bp(+) (GRCm39) |
| Type of Mutation |
missense |
| DNA Base Change (assembly) |
A to G
at 69917852 bp (GRCm39)
|
| Zygosity |
Heterozygous |
| Amino Acid Change |
Asparagine to Serine
at position 45
(N45S)
|
| Ref Sequence |
ENSEMBL: ENSMUSP00000115206
(fasta)
|
| Gene Model |
predicted gene model for transcript(s):
[ENSMUST00000018700]
[ENSMUST00000108588]
[ENSMUST00000108589]
[ENSMUST00000123687]
[ENSMUST00000124568]
[ENSMUST00000132597]
[ENSMUST00000134376]
[ENSMUST00000135916]
[ENSMUST00000133140]
[ENSMUST00000231221]
[ENSMUST00000231506]
[ENSMUST00000231628]
[ENSMUST00000232115]
[ENSMUST00000232002]
[ENSMUST00000143920]
[ENSMUST00000231415]
[ENSMUST00000232266]
[ENSMUST00000231452]
|
| AlphaFold |
Q62108 |
| Predicted Effect |
probably benign
Transcript: ENSMUST00000018700
|
| SMART Domains |
Protein: ENSMUSP00000018700
Gene: ENSMUSG00000020886
| Domain | Start | End | E-Value | Type |
|---|
|
MAGUK_N_PEST
|
10 |
64 |
1.36e-4 |
SMART |
|
PDZ
|
73 |
152 |
3.38e-21 |
SMART |
|
PDZ
|
168 |
247 |
1.12e-21 |
SMART |
|
PDZ
|
321 |
394 |
4.13e-25 |
SMART |
|
SH3
|
431 |
497 |
1.68e-9 |
SMART |
|
GuKc
|
533 |
712 |
3.65e-68 |
SMART |
|
| Predicted Effect |
noncoding transcript
Transcript: ENSMUST00000108586
|
| Predicted Effect |
probably benign
Transcript: ENSMUST00000108588
|
| SMART Domains |
Protein: ENSMUSP00000104229
Gene: ENSMUSG00000020886
| Domain | Start | End | E-Value | Type |
|---|
|
MAGUK_N_PEST
|
10 |
61 |
1e-7 |
SMART |
|
PDZ
|
70 |
149 |
3.38e-21 |
SMART |
|
PDZ
|
165 |
244 |
1.12e-21 |
SMART |
|
PDZ
|
318 |
391 |
4.13e-25 |
SMART |
|
SH3
|
428 |
494 |
1.68e-9 |
SMART |
|
GuKc
|
530 |
709 |
3.65e-68 |
SMART |
|
| Predicted Effect |
probably benign
Transcript: ENSMUST00000108589
|
| SMART Domains |
Protein: ENSMUSP00000104230
Gene: ENSMUSG00000020886
| Domain | Start | End | E-Value | Type |
|---|
|
signal peptide
|
1 |
23 |
N/A |
INTRINSIC |
|
MAGUK_N_PEST
|
53 |
107 |
1.36e-4 |
SMART |
|
PDZ
|
116 |
195 |
3.38e-21 |
SMART |
|
PDZ
|
211 |
290 |
1.12e-21 |
SMART |
|
PDZ
|
364 |
437 |
4.13e-25 |
SMART |
|
SH3
|
474 |
540 |
1.68e-9 |
SMART |
|
GuKc
|
576 |
755 |
3.65e-68 |
SMART |
|
| Predicted Effect |
probably benign
Transcript: ENSMUST00000123687
|
| SMART Domains |
Protein: ENSMUSP00000134545
Gene: ENSMUSG00000020886
| Domain | Start | End | E-Value | Type |
|---|
|
SH3
|
11 |
77 |
1.68e-9 |
SMART |
|
GuKc
|
113 |
205 |
7.37e-5 |
SMART |
|
| Predicted Effect |
probably benign
Transcript: ENSMUST00000124568
|
| SMART Domains |
Protein: ENSMUSP00000121053
Gene: ENSMUSG00000020886
| Domain | Start | End | E-Value | Type |
|---|
|
low complexity region
|
9 |
36 |
N/A |
INTRINSIC |
|
MAGUK_N_PEST
|
69 |
123 |
7.67e-4 |
SMART |
|
Pfam:PDZ
|
124 |
185 |
7.6e-17 |
PFAM |
|
| Predicted Effect |
probably benign
Transcript: ENSMUST00000132597
|
| SMART Domains |
Protein: ENSMUSP00000114165
Gene: ENSMUSG00000020886
| Domain | Start | End | E-Value | Type |
|---|
|
Pfam:MAGUK_N_PEST
|
2 |
43 |
5.8e-13 |
PFAM |
|
PDZ
|
52 |
131 |
3.38e-21 |
SMART |
|
PDZ
|
147 |
226 |
1.12e-21 |
SMART |
|
| Predicted Effect |
possibly damaging
Transcript: ENSMUST00000134376
AA Change: N45S
PolyPhen 2
Score 0.710 (Sensitivity: 0.86; Specificity: 0.92)
|
| SMART Domains |
Protein: ENSMUSP00000115206
Gene: ENSMUSG00000020886
AA Change: N45S
| Domain | Start | End | E-Value | Type |
|---|
|
MAGUK_N_PEST
|
10 |
97 |
3.39e-37 |
SMART |
|
PDZ
|
106 |
165 |
1.79e-2 |
SMART |
|
| Predicted Effect |
noncoding transcript
Transcript: ENSMUST00000138004
|
| Predicted Effect |
unknown
Transcript: ENSMUST00000232659
AA Change: N9S
|
| Predicted Effect |
probably benign
Transcript: ENSMUST00000135916
|
| SMART Domains |
Protein: ENSMUSP00000135994
Gene: ENSMUSG00000020886
| Domain | Start | End | E-Value | Type |
|---|
|
Pfam:PDZ
|
5 |
51 |
6.3e-11 |
PFAM |
|
| Predicted Effect |
noncoding transcript
Transcript: ENSMUST00000133140
|
| SMART Domains |
Protein: ENSMUSP00000126707
Gene: ENSMUSG00000020886
| Domain | Start | End | E-Value | Type |
|---|
|
PDZ
|
13 |
92 |
3.38e-21 |
SMART |
|
| Predicted Effect |
probably benign
Transcript: ENSMUST00000231221
|
| Predicted Effect |
probably benign
Transcript: ENSMUST00000231506
|
| Predicted Effect |
probably benign
Transcript: ENSMUST00000231628
|
| Predicted Effect |
probably benign
Transcript: ENSMUST00000232115
|
| Predicted Effect |
probably benign
Transcript: ENSMUST00000232002
|
| Predicted Effect |
probably benign
Transcript: ENSMUST00000143920
|
| Predicted Effect |
probably benign
Transcript: ENSMUST00000231415
|
| Predicted Effect |
probably benign
Transcript: ENSMUST00000232266
|
| Predicted Effect |
probably benign
Transcript: ENSMUST00000231452
|
| Meta Mutation Damage Score |
0.0911 |
| Coding Region Coverage |
- 1x: 99.3%
- 3x: 98.7%
- 10x: 97.4%
- 20x: 95.7%
|
| Validation Efficiency |
93% (51/55) |
| MGI Phenotype |
FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes a member of the membrane-associated guanylate kinase (MAGUK) family. It heteromultimerizes with another MAGUK protein, DLG2, and is recruited into NMDA receptor and potassium channel clusters. These two MAGUK proteins may interact at postsynaptic sites to form a multimeric scaffold for the clustering of receptors, ion channels, and associated signaling proteins. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
PHENOTYPE: Homozygotes for a targeted null mutation exhibit severely impaired spatial learning, alterations in long-term potentiation and depression, and lack of hyperalgesia responses in a neuropathic pain model. [provided by MGI curators]
|
| Allele List at MGI |
|
| Other mutations in this stock |
Total: 52 list
| Gene | Ref | Var | Chr/Loc | Mutation | Predicted Effect | Zygosity |
|---|
| Adam34l |
A |
G |
8: 44,080,200 (GRCm39) |
V8A |
possibly damaging |
Het |
| Agbl4 |
A |
T |
4: 111,423,939 (GRCm39) |
M322L |
probably benign |
Het |
| Ankrd55 |
A |
G |
13: 112,492,522 (GRCm39) |
K231R |
probably benign |
Het |
| Asap1 |
A |
C |
15: 63,999,272 (GRCm39) |
M534R |
probably damaging |
Het |
| B4galnt2 |
T |
A |
11: 95,767,140 (GRCm39) |
|
probably benign |
Het |
| Cacna1e |
A |
T |
1: 154,437,475 (GRCm39) |
|
probably null |
Het |
| Cacna1h |
T |
C |
17: 25,594,224 (GRCm39) |
I2311M |
probably benign |
Het |
| Capn2 |
G |
T |
1: 182,300,138 (GRCm39) |
D617E |
possibly damaging |
Het |
| Catsper1 |
A |
G |
19: 5,385,998 (GRCm39) |
D77G |
probably damaging |
Het |
| Cdc42bpa |
A |
T |
1: 179,922,098 (GRCm39) |
|
probably benign |
Het |
| Cdca8 |
T |
C |
4: 124,820,470 (GRCm39) |
K109E |
probably damaging |
Het |
| Dbx1 |
A |
G |
7: 49,283,242 (GRCm39) |
S223P |
probably damaging |
Het |
| Dlgap2 |
T |
G |
8: 14,872,691 (GRCm39) |
V723G |
probably benign |
Het |
| Efl1 |
C |
A |
7: 82,307,295 (GRCm39) |
Q64K |
probably damaging |
Het |
| Eml1 |
T |
A |
12: 108,472,871 (GRCm39) |
|
probably benign |
Het |
| Fbxw16 |
A |
G |
9: 109,270,117 (GRCm39) |
|
probably null |
Het |
| Gm10800 |
A |
T |
2: 98,497,379 (GRCm39) |
L80M |
probably benign |
Het |
| H4c16 |
A |
G |
6: 136,781,113 (GRCm39) |
Y89H |
probably benign |
Het |
| Insig2 |
A |
T |
1: 121,239,964 (GRCm39) |
V112E |
probably damaging |
Het |
| Kcnip3 |
A |
G |
2: 127,307,797 (GRCm39) |
S123P |
probably damaging |
Het |
| Map3k9 |
T |
C |
12: 81,780,851 (GRCm39) |
|
probably null |
Het |
| Myo16 |
T |
C |
8: 10,372,658 (GRCm39) |
V119A |
probably damaging |
Het |
| Naa25 |
G |
A |
5: 121,562,639 (GRCm39) |
V474M |
probably benign |
Het |
| Nckap1 |
A |
T |
2: 80,379,277 (GRCm39) |
V219E |
probably damaging |
Het |
| Niban1 |
A |
G |
1: 151,590,274 (GRCm39) |
I523V |
probably benign |
Het |
| Nrp1 |
G |
T |
8: 129,227,154 (GRCm39) |
|
probably null |
Het |
| Nsun4 |
A |
T |
4: 115,905,781 (GRCm39) |
D58E |
probably benign |
Het |
| Obscn |
G |
T |
11: 58,934,883 (GRCm39) |
A5249D |
probably damaging |
Het |
| Or8k53 |
A |
T |
2: 86,177,683 (GRCm39) |
H142Q |
probably benign |
Het |
| Osmr |
A |
T |
15: 6,873,874 (GRCm39) |
Y174* |
probably null |
Het |
| Pi4k2a |
T |
C |
19: 42,108,275 (GRCm39) |
|
probably null |
Het |
| Pram1 |
C |
T |
17: 33,863,878 (GRCm39) |
Q572* |
probably null |
Het |
| Prdm5 |
C |
A |
6: 65,756,158 (GRCm39) |
T25K |
probably damaging |
Het |
| Psen1 |
T |
C |
12: 83,771,439 (GRCm39) |
Y240H |
probably damaging |
Het |
| Pygl |
C |
T |
12: 70,248,666 (GRCm39) |
G318S |
probably benign |
Het |
| Rbck1 |
A |
C |
2: 152,160,371 (GRCm39) |
M436R |
probably benign |
Het |
| Resf1 |
T |
G |
6: 149,227,528 (GRCm39) |
S191R |
probably benign |
Het |
| Rmnd1 |
T |
C |
10: 4,377,488 (GRCm39) |
N64D |
possibly damaging |
Het |
| Rsph4a |
A |
G |
10: 33,784,275 (GRCm39) |
D299G |
probably damaging |
Het |
| Sema4c |
T |
C |
1: 36,590,812 (GRCm39) |
S480G |
probably benign |
Het |
| Srp68 |
T |
C |
11: 116,136,638 (GRCm39) |
D552G |
probably damaging |
Het |
| Syde2 |
G |
T |
3: 145,707,764 (GRCm39) |
A568S |
probably damaging |
Het |
| Szrd1 |
G |
T |
4: 140,867,092 (GRCm39) |
|
probably null |
Het |
| Szt2 |
A |
T |
4: 118,226,813 (GRCm39) |
|
probably null |
Het |
| Tbc1d31 |
A |
G |
15: 57,818,797 (GRCm39) |
E800G |
probably benign |
Het |
| Tmprss11d |
C |
A |
5: 86,457,122 (GRCm39) |
|
probably null |
Het |
| Usp37 |
A |
G |
1: 74,480,720 (GRCm39) |
V895A |
probably damaging |
Het |
| Vmn1r19 |
T |
C |
6: 57,382,026 (GRCm39) |
I193T |
probably benign |
Het |
| Vmn2r102 |
T |
C |
17: 19,897,834 (GRCm39) |
V283A |
probably benign |
Het |
| Vps13d |
C |
T |
4: 144,814,811 (GRCm39) |
G3180D |
probably damaging |
Het |
| Xirp2 |
A |
C |
2: 67,344,821 (GRCm39) |
D2354A |
probably benign |
Het |
| Zc3h14 |
T |
A |
12: 98,723,465 (GRCm39) |
|
probably null |
Het |
|
| Other mutations in Dlg4 |
| Allele | Source | Chr | Coord | Type | Predicted Effect | PPH Score |
|---|
|
IGL01753:Dlg4
|
APN |
11 |
69,932,173 (GRCm39) |
missense |
probably damaging |
1.00 |
|
IGL02260:Dlg4
|
APN |
11 |
69,933,093 (GRCm39) |
missense |
probably damaging |
1.00 |
|
IGL03097:Dlg4
|
UTSW |
11 |
69,933,028 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R0103:Dlg4
|
UTSW |
11 |
69,922,019 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R0103:Dlg4
|
UTSW |
11 |
69,922,019 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R0628:Dlg4
|
UTSW |
11 |
69,922,610 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R0734:Dlg4
|
UTSW |
11 |
69,933,531 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R1587:Dlg4
|
UTSW |
11 |
69,922,572 (GRCm39) |
missense |
possibly damaging |
0.88 |
|
R1946:Dlg4
|
UTSW |
11 |
69,930,401 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R2190:Dlg4
|
UTSW |
11 |
69,933,430 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R2259:Dlg4
|
UTSW |
11 |
69,922,196 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R2289:Dlg4
|
UTSW |
11 |
69,917,752 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R2411:Dlg4
|
UTSW |
11 |
69,932,755 (GRCm39) |
critical splice donor site |
probably null |
|
|
R3161:Dlg4
|
UTSW |
11 |
69,908,051 (GRCm39) |
missense |
probably damaging |
0.99 |
|
R4059:Dlg4
|
UTSW |
11 |
69,917,909 (GRCm39) |
missense |
probably benign |
|
|
R4782:Dlg4
|
UTSW |
11 |
69,917,780 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R4910:Dlg4
|
UTSW |
11 |
69,921,751 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R5557:Dlg4
|
UTSW |
11 |
69,933,106 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R5996:Dlg4
|
UTSW |
11 |
69,908,057 (GRCm39) |
missense |
probably benign |
0.00 |
|
R6649:Dlg4
|
UTSW |
11 |
69,914,779 (GRCm39) |
unclassified |
probably benign |
|
|
R6653:Dlg4
|
UTSW |
11 |
69,914,779 (GRCm39) |
unclassified |
probably benign |
|
|
R7155:Dlg4
|
UTSW |
11 |
69,908,042 (GRCm39) |
start codon destroyed |
probably null |
0.00 |
|
R7284:Dlg4
|
UTSW |
11 |
69,932,908 (GRCm39) |
nonsense |
probably null |
|
|
R7683:Dlg4
|
UTSW |
11 |
69,930,680 (GRCm39) |
missense |
possibly damaging |
0.95 |
|
R7976:Dlg4
|
UTSW |
11 |
69,930,008 (GRCm39) |
missense |
probably damaging |
0.99 |
|
R8051:Dlg4
|
UTSW |
11 |
69,922,468 (GRCm39) |
unclassified |
probably benign |
|
|
R8408:Dlg4
|
UTSW |
11 |
69,933,078 (GRCm39) |
missense |
possibly damaging |
0.81 |
|
R8431:Dlg4
|
UTSW |
11 |
69,930,388 (GRCm39) |
missense |
probably benign |
0.36 |
|
R9283:Dlg4
|
UTSW |
11 |
69,922,617 (GRCm39) |
nonsense |
probably null |
|
|
R9451:Dlg4
|
UTSW |
11 |
69,922,065 (GRCm39) |
missense |
probably damaging |
1.00 |
|
Z1088:Dlg4
|
UTSW |
11 |
69,921,956 (GRCm39) |
missense |
probably damaging |
1.00 |
|
Z1176:Dlg4
|
UTSW |
11 |
69,932,746 (GRCm39) |
missense |
probably benign |
0.00 |
|
| Predicted Primers |
PCR Primer
(F):5'- AGGAGTTGGTCAGGCCCTAG -3'
(R):5'- AGGAGTCCAAGGCCCTGATG -3'
Sequencing Primer
(F):5'- CACTGCACTGCTCTGGGAAAAAG -3'
(R):5'- CTGATGCCAGGCCACCC -3'
|
| Posted On |
2016-07-22 |
Incidental Mutation