Mutagenetix > Incidental Mutation

Incidental Mutation 'R5223:Or7g35'

402434

Institutional Source

Beutler Lab

Gene Symbol

Or7g35

Ensembl Gene

ENSMUSG00000043087

Gene Name

olfactory receptor family 7 subfamily G member 35

Synonyms

Olfr855, MOR148-1, GA_x6K02T2PVTD-13330461-13331399

MMRRC Submission

042796-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.132) question?

Stock #

R5223 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

19495807-19496798 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 19496322 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Valine to Alanine at position 163 (V163A)

Ref Sequence

ENSEMBL: ENSMUSP00000150218 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000061693] [ENSMUST00000215587]

AlphaFold

Q7TRF8

Predicted Effect

probably benign
Transcript: ENSMUST00000061693
AA Change: V163A

PolyPhen 2 Score 0.157 (Sensitivity: 0.92; Specificity: 0.87)

SMART Domains

Protein: ENSMUSP00000054790
Gene: ENSMUSG00000043087
AA Change: V163A

Domain	Start	End	E-Value	Type
Pfam:7tm_4	33	310	1.7e-54	PFAM
Pfam:7TM_GPCR_Srsx	37	182	5.2e-8	PFAM
Pfam:7tm_1	43	292	2.5e-24	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000215587
AA Change: V163A

PolyPhen 2 Score 0.157 (Sensitivity: 0.92; Specificity: 0.87)

Coding Region Coverage

1x: 99.2%
3x: 98.4%
10x: 96.6%
20x: 93.2%

Validation Efficiency

MGI Phenotype

FUNCTION: Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]

Allele List at MGI

Other mutations in this stock

Total: 70 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Aatk	C	T	11: 119,904,278 (GRCm39)	V273M	possibly damaging	Het
Acin1	CCGC	CC	14: 54,880,398 (GRCm39)		probably null	Het
Acvr1b	T	A	15: 101,091,857 (GRCm39)	C46S	probably damaging	Het
Ahi1	A	T	10: 20,846,818 (GRCm39)	H416L	possibly damaging	Het
Aspm	T	A	1: 139,406,072 (GRCm39)	L1653Q	probably damaging	Het
Cacna1a	G	A	8: 85,313,824 (GRCm39)	V1533M	possibly damaging	Het
Ccdc33	C	T	9: 57,940,267 (GRCm39)	E502K	possibly damaging	Het
Ctnnd1	C	T	2: 84,447,133 (GRCm39)	V371M	probably damaging	Het
Cyp2a12	A	T	7: 26,735,888 (GRCm39)		probably null	Het
Dnai4	T	A	4: 102,906,600 (GRCm39)	S738C	possibly damaging	Het
Ep400	A	G	5: 110,816,496 (GRCm39)	V2675A	probably damaging	Het
Foxh1	T	A	15: 76,552,929 (GRCm39)		probably null	Het
Gad1-ps	G	A	10: 99,281,009 (GRCm39)		noncoding transcript	Het
Gpnmb	C	T	6: 49,033,139 (GRCm39)	T539M	probably benign	Het
Hdac1-ps	A	G	17: 78,799,867 (GRCm39)	E286G	probably benign	Het
Hspa9	A	G	18: 35,085,724 (GRCm39)		probably null	Het
Hspg2	T	C	4: 137,271,225 (GRCm39)	L2454P	probably damaging	Het
Ift140	T	A	17: 25,254,786 (GRCm39)	I422N	probably benign	Het
Igkv6-32	T	C	6: 70,051,207 (GRCm39)	S50G	probably benign	Het
Il23r	T	A	6: 67,463,154 (GRCm39)	Y113F	probably benign	Het
Kcnt1	A	G	2: 25,793,434 (GRCm39)	D636G	probably benign	Het
Klhl41	G	A	2: 69,510,171 (GRCm39)	W569*	probably null	Het
Klra4	T	A	6: 130,039,110 (GRCm39)	D94V	probably damaging	Het
Lca5	T	A	9: 83,280,666 (GRCm39)	H378L	probably benign	Het
Lhx8	T	A	3: 154,027,281 (GRCm39)	T254S	probably damaging	Het
Lrch3	C	T	16: 32,734,767 (GRCm39)	R86W	probably damaging	Het
Lrp2	T	A	2: 69,354,397 (GRCm39)	N477I	probably damaging	Het
Man2a1	T	A	17: 65,019,266 (GRCm39)	I710K	probably benign	Het
Ncor1	A	T	11: 62,229,826 (GRCm39)	Y881N	probably damaging	Het
Nhsl1	T	A	10: 18,402,074 (GRCm39)	V1100E	probably damaging	Het
Oprk1	T	C	1: 5,659,519 (GRCm39)	V83A	probably benign	Het
Or4p8	T	C	2: 88,727,678 (GRCm39)	T88A	probably benign	Het
Or5p62	A	G	7: 107,771,915 (GRCm39)	V12A	probably benign	Het
Pacs1	C	T	19: 5,195,169 (GRCm39)	V472I	probably benign	Het
Pard3b	T	C	1: 62,383,272 (GRCm39)	Y789H	probably damaging	Het
Pcdha2	T	C	18: 37,073,844 (GRCm39)	Y492H	probably damaging	Het
Pcnt	T	C	10: 76,216,106 (GRCm39)	N2261D	probably damaging	Het
Pex5l	A	T	3: 33,012,945 (GRCm39)	S15T	probably damaging	Het
Plekhg4	A	G	8: 106,105,581 (GRCm39)	N682S	probably benign	Het
Poc5	A	T	13: 96,539,463 (GRCm39)	M335L	probably benign	Het
Polr1a	C	T	6: 71,944,891 (GRCm39)	R1316W	possibly damaging	Het
Pp2d1	T	C	17: 53,814,873 (GRCm39)	H617R	probably benign	Het
Pramel24	T	A	4: 143,454,591 (GRCm39)	S296R	probably benign	Het
Prpsap1	T	C	11: 116,378,974 (GRCm39)	K65E	probably benign	Het
Ptgfrn	T	C	3: 100,952,909 (GRCm39)	E775G	probably benign	Het
Ptprc	T	A	1: 138,045,600 (GRCm39)	I87F	probably benign	Het
Rbbp8	C	A	18: 11,854,747 (GRCm39)	A324E	probably benign	Het
Rfx6	G	T	10: 51,554,092 (GRCm39)	G63*	probably null	Het
Rpl37a	T	C	1: 72,751,308 (GRCm39)	M47T	probably benign	Het
Samm50	T	G	15: 84,084,831 (GRCm39)	N187K	probably benign	Het
Skic2	A	G	17: 35,064,142 (GRCm39)		probably null	Het
Slamf9	T	C	1: 172,303,799 (GRCm39)	I48T	possibly damaging	Het
Slc2a12	A	G	10: 22,577,931 (GRCm39)	K576E	probably damaging	Het
Slc4a10	G	A	2: 62,083,710 (GRCm39)	G388S	probably damaging	Het
Smtn	G	T	11: 3,479,530 (GRCm39)	N512K	probably benign	Het
Sntb1	C	G	15: 55,506,191 (GRCm39)	G461R	probably damaging	Het
Sspo	A	G	6: 48,455,258 (GRCm39)	Y3040C	probably damaging	Het
Stat1	T	A	1: 52,183,401 (GRCm39)	V389E	probably damaging	Het
Sult5a1	A	T	8: 123,872,161 (GRCm39)	M227K	probably damaging	Het
Thsd4	T	C	9: 59,964,325 (GRCm39)	D389G	probably damaging	Het
Tnxb	T	C	17: 34,923,052 (GRCm39)	V2545A	possibly damaging	Het
Tpo	T	A	12: 30,142,589 (GRCm39)	I712F	probably damaging	Het
Trim62	T	C	4: 128,803,204 (GRCm39)	V418A	probably damaging	Het
Uqcrc1	C	A	9: 108,771,224 (GRCm39)	H95N	probably damaging	Het
Vmn2r114	ATTT	ATT	17: 23,509,906 (GRCm39)		probably null	Het
Vmn2r66	T	C	7: 84,657,093 (GRCm39)	D104G	probably benign	Het
Wdr26	T	C	1: 181,015,251 (GRCm39)	I371V	probably benign	Het
Zfp273	T	G	13: 67,974,298 (GRCm39)	C475W	probably damaging	Het
Zfp738	G	T	13: 67,821,182 (GRCm39)	T55K	probably damaging	Het
Zmym2	A	G	14: 57,183,971 (GRCm39)	I978V	probably benign	Het

Other mutations in Or7g35

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01020:Or7g35	APN	9	19,496,616 (GRCm39)	missense	possibly damaging	0.74
IGL01405:Or7g35	APN	9	19,496,501 (GRCm39)	missense	probably benign	0.23
IGL01775:Or7g35	APN	9	19,496,001 (GRCm39)	missense	probably benign	0.04
IGL01920:Or7g35	APN	9	19,496,318 (GRCm39)	missense	probably benign	0.01
R0501:Or7g35	UTSW	9	19,495,914 (GRCm39)	missense	probably damaging	1.00
R0600:Or7g35	UTSW	9	19,496,600 (GRCm39)	missense	possibly damaging	0.47
R0667:Or7g35	UTSW	9	19,496,743 (GRCm39)	missense	probably benign
R1769:Or7g35	UTSW	9	19,496,682 (GRCm39)	missense	probably damaging	0.98
R3117:Or7g35	UTSW	9	19,496,237 (GRCm39)	missense	probably damaging	0.99
R4002:Or7g35	UTSW	9	19,496,010 (GRCm39)	missense	probably damaging	1.00
R4003:Or7g35	UTSW	9	19,496,010 (GRCm39)	missense	probably damaging	1.00
R4043:Or7g35	UTSW	9	19,496,291 (GRCm39)	missense	probably benign	0.16
R4243:Or7g35	UTSW	9	19,495,854 (GRCm39)	missense	probably damaging	1.00
R4672:Or7g35	UTSW	9	19,496,726 (GRCm39)	missense	possibly damaging	0.74
R4673:Or7g35	UTSW	9	19,496,726 (GRCm39)	missense	possibly damaging	0.74
R4959:Or7g35	UTSW	9	19,496,504 (GRCm39)	missense	probably benign
R4973:Or7g35	UTSW	9	19,496,504 (GRCm39)	missense	probably benign
R5681:Or7g35	UTSW	9	19,496,195 (GRCm39)	missense	probably damaging	1.00
R6005:Or7g35	UTSW	9	19,496,181 (GRCm39)	missense	probably benign	0.45
R6017:Or7g35	UTSW	9	19,496,730 (GRCm39)	missense	probably benign	0.00
R6145:Or7g35	UTSW	9	19,496,184 (GRCm39)	missense	probably benign	0.02
R6615:Or7g35	UTSW	9	19,496,285 (GRCm39)	missense	probably benign	0.05
R6771:Or7g35	UTSW	9	19,496,675 (GRCm39)	missense	probably benign	0.16
R6969:Or7g35	UTSW	9	19,495,886 (GRCm39)	missense	possibly damaging	0.77
R7239:Or7g35	UTSW	9	19,496,487 (GRCm39)	missense	probably damaging	1.00
R7313:Or7g35	UTSW	9	19,495,938 (GRCm39)	missense	probably damaging	1.00
R7361:Or7g35	UTSW	9	19,495,856 (GRCm39)	missense	probably benign	0.00
R8112:Or7g35	UTSW	9	19,496,020 (GRCm39)	missense	probably benign	0.44
R8470:Or7g35	UTSW	9	19,496,265 (GRCm39)	missense	probably damaging	0.99
R9155:Or7g35	UTSW	9	19,496,379 (GRCm39)	missense	probably benign	0.00
R9187:Or7g35	UTSW	9	19,495,950 (GRCm39)	missense	probably benign	0.03
R9422:Or7g35	UTSW	9	19,495,968 (GRCm39)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- GAGCATCACTTACACAGACTGC -3'
(R):5'- CAAGTGAGTCCCGCAAGTAG -3'

Sequencing Primer
(F):5'- AGACTGCCTCACACAGCTCTTC -3'
(R):5'- TCCCGCAAGTAGAGAAGGCTTTATAC -3'

Posted On

2016-07-22