Mutagenetix > Incidental Mutation

Incidental Mutation 'R5218:Vmn2r77'

403602

Institutional Source

Beutler Lab

Gene Symbol

Vmn2r77

Ensembl Gene

ENSMUSG00000090949

Gene Name

vomeronasal 2, receptor 77

Synonyms

EG546983

MMRRC Submission

042791-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.079) question?

Stock #

R5218 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

86444349-86461240 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to A at 86451341 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Methionine to Lysine at position 409 (M409K)

Ref Sequence

ENSEMBL: ENSMUSP00000129540 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000164996]

AlphaFold

L7N2B7

Predicted Effect

probably damaging
Transcript: ENSMUST00000164996
AA Change: M409K

PolyPhen 2 Score 0.976 (Sensitivity: 0.76; Specificity: 0.96)

SMART Domains

Protein: ENSMUSP00000129540
Gene: ENSMUSG00000090949
AA Change: M409K

Domain	Start	End	E-Value	Type
signal peptide	1	21	N/A	INTRINSIC
Pfam:ANF_receptor	78	467	1.4e-30	PFAM
Pfam:NCD3G	510	562	1e-20	PFAM
Pfam:7tm_3	594	830	2.6e-52	PFAM

Meta Mutation Damage Score

0.6467

Coding Region Coverage

1x: 99.3%
3x: 98.7%
10x: 97.5%
20x: 95.9%

Validation Efficiency

100% (60/60)

Allele List at MGI

Other mutations in this stock

Total: 56 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
4921524L21Rik	T	A	18: 6,629,628 (GRCm39)	N230K	possibly damaging	Het
4933428M09Rik	G	T	X: 138,080,282 (GRCm39)	G16*	probably null	Het
Albfm1	A	G	5: 90,729,777 (GRCm39)	K400R	probably benign	Het
Amigo1	A	G	3: 108,095,086 (GRCm39)		probably null	Het
Arhgap30	A	T	1: 171,236,328 (GRCm39)	T901S	probably benign	Het
Arpp21	T	C	9: 111,972,499 (GRCm39)	D264G	probably damaging	Het
Ccbe1	C	T	18: 66,216,229 (GRCm39)	G165S	probably damaging	Het
Celsr1	T	C	15: 85,816,585 (GRCm39)	D1786G	probably damaging	Het
Cntn1	T	C	15: 92,237,430 (GRCm39)	L1008P	unknown	Het
Dclk2	T	A	3: 86,712,985 (GRCm39)	D489V	probably damaging	Het
Dip2b	T	A	15: 100,052,177 (GRCm39)	D259E	probably benign	Het
Disp3	T	C	4: 148,327,333 (GRCm39)	S1184G	possibly damaging	Het
Dync1li2	C	T	8: 105,169,179 (GRCm39)	W36*	probably null	Het
Ece2	A	T	16: 20,437,290 (GRCm39)	M211L	probably benign	Het
Edn3	C	T	2: 174,603,345 (GRCm39)	A31V	probably benign	Het
Enpp2	T	C	15: 54,750,982 (GRCm39)	Q266R	possibly damaging	Het
Fabp1	C	A	6: 71,176,944 (GRCm39)	Q12K	probably damaging	Het
Flt1	G	A	5: 147,618,738 (GRCm39)	T199M	probably damaging	Het
Galns	T	A	8: 123,325,328 (GRCm39)	I275F	probably damaging	Het
Gapvd1	T	C	2: 34,618,488 (GRCm39)	D295G	probably benign	Het
Gatb	A	G	3: 85,511,751 (GRCm39)	I169V	probably benign	Het
Gpd1	T	A	15: 99,618,011 (GRCm39)	I109N	probably damaging	Het
Gps2	T	C	11: 69,807,121 (GRCm39)		probably null	Het
Hsd17b12	T	C	2: 93,913,608 (GRCm39)	N99D	probably benign	Het
Itga3	A	T	11: 94,953,574 (GRCm39)	V256E	probably benign	Het
Kcnma1	T	A	14: 23,513,253 (GRCm39)	Y527F	probably damaging	Het
Lamc1	C	A	1: 153,103,442 (GRCm39)	V1375L	probably damaging	Het
Lrp1	C	A	10: 127,384,488 (GRCm39)	E3580D	probably damaging	Het
Ltbp4	AATTCAGGCCAAGGCTGGGATTCAGGCCGAGGCCGGGATTCAGGCCTAGGCTGGGATTCAGGC	AATTCAGGCCTAGGCTGGGATTCAGGC	7: 27,026,736 (GRCm39)		probably benign	Het
Medag	A	T	5: 149,345,719 (GRCm39)		probably benign	Het
Ncdn	C	A	4: 126,644,603 (GRCm39)	R73L	probably benign	Het
Nr4a1	T	C	15: 101,170,034 (GRCm39)	V339A	probably benign	Het
Ocln	G	T	13: 100,642,822 (GRCm39)	P420Q	probably damaging	Het
Or5ac24	A	T	16: 59,165,270 (GRCm39)	S265T	probably benign	Het
Pank4	C	T	4: 155,064,185 (GRCm39)	T681I	probably benign	Het
Pcdhb6	T	A	18: 37,467,388 (GRCm39)	V103E	possibly damaging	Het
Pcsk6	T	C	7: 65,675,036 (GRCm39)	F469S	probably benign	Het
Phrf1	A	G	7: 140,841,214 (GRCm39)	N1353S	possibly damaging	Het
Pou6f2	A	G	13: 18,326,586 (GRCm39)	I72T	probably damaging	Het
Ppig	T	C	2: 69,563,127 (GRCm39)		probably benign	Het
Ptprh	T	C	7: 4,600,919 (GRCm39)	S153G	probably benign	Het
Rab11b	T	C	17: 33,967,924 (GRCm39)	N91S	probably benign	Het
Scara5	CG	C	14: 65,997,111 (GRCm39)		probably null	Het
Slc2a9	A	G	5: 38,610,524 (GRCm39)	S92P	probably damaging	Het
Slit3	G	A	11: 35,575,002 (GRCm39)		probably null	Het
Sox5	T	C	6: 143,906,616 (GRCm39)	I280V	possibly damaging	Het
Stam2	G	A	2: 52,626,305 (GRCm39)		probably benign	Het
Tdrd9	C	T	12: 112,029,909 (GRCm39)		probably benign	Het
Tmem273	T	A	14: 32,538,793 (GRCm39)		probably null	Het
Tmem74	A	T	15: 43,730,640 (GRCm39)	N134K	possibly damaging	Het
Ttn	A	T	2: 76,641,587 (GRCm39)	L5176Q	possibly damaging	Het
Txnl1	T	G	18: 63,812,538 (GRCm39)	K85N	probably benign	Het
Wipf1	T	C	2: 73,274,812 (GRCm39)	D53G	probably damaging	Het
Wnt10a	G	A	1: 74,832,754 (GRCm39)	V116I	probably benign	Het
Xpot	T	A	10: 121,455,043 (GRCm39)	D33V	probably damaging	Het
Znrf3	C	T	11: 5,231,519 (GRCm39)	V665M	possibly damaging	Het

Other mutations in Vmn2r77

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00954:Vmn2r77	APN	7	86,449,975 (GRCm39)	missense	probably benign	0.06
IGL01105:Vmn2r77	APN	7	86,460,872 (GRCm39)	missense	probably damaging	0.99
IGL01367:Vmn2r77	APN	7	86,461,124 (GRCm39)	missense	probably damaging	0.98
IGL01634:Vmn2r77	APN	7	86,460,857 (GRCm39)	missense	probably benign
IGL01805:Vmn2r77	APN	7	86,460,395 (GRCm39)	missense	probably benign	0.18
IGL01868:Vmn2r77	APN	7	86,452,224 (GRCm39)	missense	probably benign	0.00
IGL01980:Vmn2r77	APN	7	86,450,678 (GRCm39)	missense	probably benign	0.14
IGL02055:Vmn2r77	APN	7	86,450,763 (GRCm39)	missense	probably benign	0.00
IGL02066:Vmn2r77	APN	7	86,452,836 (GRCm39)	nonsense	probably null
IGL02185:Vmn2r77	APN	7	86,444,360 (GRCm39)	missense	unknown
IGL02200:Vmn2r77	APN	7	86,451,187 (GRCm39)	missense	probably benign	0.04
IGL02336:Vmn2r77	APN	7	86,451,224 (GRCm39)	missense	probably damaging	0.99
IGL02445:Vmn2r77	APN	7	86,452,848 (GRCm39)	nonsense	probably null
IGL02557:Vmn2r77	APN	7	86,444,342 (GRCm39)	unclassified	probably benign
IGL02659:Vmn2r77	APN	7	86,449,979 (GRCm39)	missense	probably benign	0.32
IGL02978:Vmn2r77	APN	7	86,460,555 (GRCm39)	missense	probably benign
IGL03180:Vmn2r77	APN	7	86,450,843 (GRCm39)	missense	possibly damaging	0.85
IGL03255:Vmn2r77	APN	7	86,461,131 (GRCm39)	missense	probably benign	0.04
IGL03273:Vmn2r77	APN	7	86,460,494 (GRCm39)	missense	probably damaging	0.99
R0046:Vmn2r77	UTSW	7	86,451,146 (GRCm39)	missense	possibly damaging	0.73
R0047:Vmn2r77	UTSW	7	86,460,858 (GRCm39)	missense	probably benign	0.01
R0066:Vmn2r77	UTSW	7	86,449,964 (GRCm39)	missense	probably benign	0.17
R0066:Vmn2r77	UTSW	7	86,449,964 (GRCm39)	missense	probably benign	0.17
R0389:Vmn2r77	UTSW	7	86,450,702 (GRCm39)	missense	probably benign	0.29
R0635:Vmn2r77	UTSW	7	86,460,383 (GRCm39)	missense	probably benign
R0689:Vmn2r77	UTSW	7	86,460,872 (GRCm39)	missense	probably damaging	0.99
R0827:Vmn2r77	UTSW	7	86,451,224 (GRCm39)	missense	probably damaging	1.00
R1167:Vmn2r77	UTSW	7	86,450,954 (GRCm39)	missense	probably benign	0.02
R1228:Vmn2r77	UTSW	7	86,450,242 (GRCm39)	critical splice donor site	probably null
R1353:Vmn2r77	UTSW	7	86,451,394 (GRCm39)	missense	probably benign	0.29
R1392:Vmn2r77	UTSW	7	86,450,830 (GRCm39)	missense	probably benign	0.00
R1392:Vmn2r77	UTSW	7	86,450,830 (GRCm39)	missense	probably benign	0.00
R1613:Vmn2r77	UTSW	7	86,460,356 (GRCm39)	missense	probably damaging	1.00
R1654:Vmn2r77	UTSW	7	86,461,123 (GRCm39)	missense	probably damaging	1.00
R1742:Vmn2r77	UTSW	7	86,444,543 (GRCm39)	missense	probably benign	0.35
R1827:Vmn2r77	UTSW	7	86,450,821 (GRCm39)	missense	probably damaging	0.99
R1911:Vmn2r77	UTSW	7	86,461,001 (GRCm39)	missense	probably damaging	1.00
R1974:Vmn2r77	UTSW	7	86,449,964 (GRCm39)	missense	probably benign	0.17
R2008:Vmn2r77	UTSW	7	86,450,921 (GRCm39)	missense	probably benign	0.31
R2093:Vmn2r77	UTSW	7	86,450,702 (GRCm39)	missense	probably benign	0.29
R2143:Vmn2r77	UTSW	7	86,461,152 (GRCm39)	missense	probably damaging	1.00
R2269:Vmn2r77	UTSW	7	86,460,897 (GRCm39)	missense	probably benign	0.03
R2972:Vmn2r77	UTSW	7	86,452,893 (GRCm39)	missense	probably benign	0.01
R2974:Vmn2r77	UTSW	7	86,452,893 (GRCm39)	missense	probably benign	0.01
R3037:Vmn2r77	UTSW	7	86,450,191 (GRCm39)	missense	probably benign
R3694:Vmn2r77	UTSW	7	86,450,044 (GRCm39)	missense	probably damaging	1.00
R3695:Vmn2r77	UTSW	7	86,450,044 (GRCm39)	missense	probably damaging	1.00
R3805:Vmn2r77	UTSW	7	86,444,368 (GRCm39)	nonsense	probably null
R3870:Vmn2r77	UTSW	7	86,461,050 (GRCm39)	missense	probably damaging	1.00
R4732:Vmn2r77	UTSW	7	86,450,195 (GRCm39)	missense	probably benign	0.00
R4733:Vmn2r77	UTSW	7	86,450,195 (GRCm39)	missense	probably benign	0.00
R5009:Vmn2r77	UTSW	7	86,451,015 (GRCm39)	missense	possibly damaging	0.82
R5201:Vmn2r77	UTSW	7	86,460,846 (GRCm39)	missense	probably damaging	0.98
R5469:Vmn2r77	UTSW	7	86,451,271 (GRCm39)	missense	probably benign	0.01
R5673:Vmn2r77	UTSW	7	86,461,214 (GRCm39)	missense	probably benign	0.05
R5771:Vmn2r77	UTSW	7	86,461,235 (GRCm39)	missense	probably benign	0.06
R5832:Vmn2r77	UTSW	7	86,460,670 (GRCm39)	nonsense	probably null
R5899:Vmn2r77	UTSW	7	86,460,924 (GRCm39)	missense	probably damaging	1.00
R6151:Vmn2r77	UTSW	7	86,450,878 (GRCm39)	missense	probably benign	0.00
R6182:Vmn2r77	UTSW	7	86,460,957 (GRCm39)	missense	probably damaging	1.00
R6326:Vmn2r77	UTSW	7	86,451,031 (GRCm39)	missense	probably benign
R6419:Vmn2r77	UTSW	7	86,460,767 (GRCm39)	missense	probably damaging	0.99
R6549:Vmn2r77	UTSW	7	86,450,065 (GRCm39)	missense	probably benign	0.06
R6874:Vmn2r77	UTSW	7	86,451,286 (GRCm39)	missense	probably benign	0.00
R6972:Vmn2r77	UTSW	7	86,452,202 (GRCm39)	missense	probably damaging	1.00
R7056:Vmn2r77	UTSW	7	86,451,023 (GRCm39)	missense	probably benign	0.06
R7185:Vmn2r77	UTSW	7	86,451,035 (GRCm39)	missense	probably benign	0.00
R7261:Vmn2r77	UTSW	7	86,460,518 (GRCm39)	nonsense	probably null
R7298:Vmn2r77	UTSW	7	86,449,979 (GRCm39)	missense	probably benign	0.00
R7662:Vmn2r77	UTSW	7	86,460,492 (GRCm39)	nonsense	probably null
R8182:Vmn2r77	UTSW	7	86,460,801 (GRCm39)	missense	probably damaging	1.00
R8327:Vmn2r77	UTSW	7	86,450,680 (GRCm39)	missense	probably benign	0.08
R8387:Vmn2r77	UTSW	7	86,450,947 (GRCm39)	missense	probably benign	0.00
R8825:Vmn2r77	UTSW	7	86,452,855 (GRCm39)	missense	probably benign
R8898:Vmn2r77	UTSW	7	86,444,430 (GRCm39)	missense	probably damaging	1.00
R8973:Vmn2r77	UTSW	7	86,452,150 (GRCm39)	missense	possibly damaging	0.93
R9258:Vmn2r77	UTSW	7	86,452,302 (GRCm39)	missense	possibly damaging	0.88
R9338:Vmn2r77	UTSW	7	86,460,994 (GRCm39)	missense	probably damaging	1.00
R9358:Vmn2r77	UTSW	7	86,452,236 (GRCm39)	missense	probably benign	0.00
R9377:Vmn2r77	UTSW	7	86,444,442 (GRCm39)	missense	probably benign	0.05
R9404:Vmn2r77	UTSW	7	86,451,247 (GRCm39)	missense	probably benign
R9673:Vmn2r77	UTSW	7	86,450,171 (GRCm39)	missense	possibly damaging	0.75
R9679:Vmn2r77	UTSW	7	86,460,741 (GRCm39)	missense	probably benign	0.07

Predicted Primers

PCR Primer
(F):5'- TGGCTAAACTGTGGTGGACTTAC -3'
(R):5'- CATCATATGCCTCTTTTGGATTTGG -3'

Sequencing Primer
(F):5'- CTAAACTGTGGTGGACTTACTTTAAG -3'
(R):5'- TGGATTTGGAGAGTCTAGAAGTAC -3'

Posted On

2016-07-22