Mutagenetix > Incidental Mutation

Incidental Mutation 'R5279:Vmn1r65'

404106

Institutional Source

Beutler Lab

Gene Symbol

Vmn1r65

Ensembl Gene

ENSMUSG00000066850

Gene Name

vomeronasal 1 receptor 65

Synonyms

V1rd6, V3R6

MMRRC Submission

042839-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.056) question?

Stock #

R5279 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

6010749-6014009 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to G at 6011754 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Valine to Alanine at position 160 (V160A)

Ref Sequence

ENSEMBL: ENSMUSP00000083520 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000086338]

AlphaFold

Q9EPS7

Predicted Effect

probably damaging
Transcript: ENSMUST00000086338
AA Change: V160A

PolyPhen 2 Score 0.996 (Sensitivity: 0.55; Specificity: 0.98)

SMART Domains

Protein: ENSMUSP00000083520
Gene: ENSMUSG00000066850
AA Change: V160A

Domain	Start	End	E-Value	Type
Pfam:TAS2R	20	308	7.3e-14	PFAM
Pfam:7tm_1	42	301	1.6e-6	PFAM
Pfam:V1R	53	308	5.6e-15	PFAM

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000227656

Meta Mutation Damage Score

0.4840

Coding Region Coverage

1x: 99.3%
3x: 98.7%
10x: 97.3%
20x: 95.4%

Validation Efficiency

99% (73/74)

Allele List at MGI

Other mutations in this stock

Total: 69 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Abca17	C	A	17: 24,508,388 (GRCm39)	G1049V	probably damaging	Het
Afdn	G	A	17: 14,109,214 (GRCm39)	R1579H	probably damaging	Het
Ankrd28	T	A	14: 31,456,963 (GRCm39)	N386Y	probably damaging	Het
Atf7ip	T	A	6: 136,580,377 (GRCm39)	Y1100*	probably null	Het
Atp9b	T	A	18: 80,956,073 (GRCm39)	E3V	probably damaging	Het
AW554918	T	G	18: 25,308,488 (GRCm39)	D60E	possibly damaging	Het
Baz2b	G	T	2: 59,762,496 (GRCm39)	Q927K	probably damaging	Het
Birc6	A	G	17: 74,957,042 (GRCm39)	R3659G	probably damaging	Het
C8a	T	G	4: 104,703,185 (GRCm39)	N291H	probably damaging	Het
Calcoco1	G	A	15: 102,619,420 (GRCm39)	L390F	probably damaging	Het
Carmil3	A	G	14: 55,739,028 (GRCm39)	D894G	probably damaging	Het
Cdc20	A	G	4: 118,290,711 (GRCm39)	Y430H	probably damaging	Het
Cdc6	A	T	11: 98,803,088 (GRCm39)	I316F	probably damaging	Het
Ceacam23	T	A	7: 17,607,214 (GRCm39)		noncoding transcript	Het
Cenpu	T	A	8: 47,031,945 (GRCm39)		probably null	Het
Csmd2	T	A	4: 128,350,707 (GRCm39)	V1592D	probably benign	Het
Csmd3	G	T	15: 48,655,340 (GRCm39)		probably null	Het
Dnah7c	T	C	1: 46,558,429 (GRCm39)	F512L	probably benign	Het
Fam136a	T	A	6: 86,343,686 (GRCm39)	L61Q	probably damaging	Het
Fpgs	A	C	2: 32,582,779 (GRCm39)		probably benign	Het
Fzd4	G	A	7: 89,056,881 (GRCm39)	M309I	probably benign	Het
Gad2	A	G	2: 22,563,969 (GRCm39)	T391A	probably benign	Het
Gm11563	A	T	11: 99,549,539 (GRCm39)	S72T	unknown	Het
Gon4l	A	G	3: 88,794,944 (GRCm39)	I716V	probably benign	Het
Itga9	T	C	9: 118,457,273 (GRCm39)	V128A	probably damaging	Het
Kat6a	CGCAGCAGCAGCAGCAGCA	CGCAGCAGCAGCA	8: 23,429,664 (GRCm39)		probably benign	Het
Lrrc9	T	A	12: 72,542,368 (GRCm39)	D1063E	possibly damaging	Het
Lyst	T	A	13: 13,823,387 (GRCm39)	L1453*	probably null	Het
Mcm3ap	T	C	10: 76,343,373 (GRCm39)	V1755A	probably damaging	Het
Mrc1	T	C	2: 14,314,869 (GRCm39)	S985P	probably damaging	Het
Mst1	A	C	9: 107,959,414 (GRCm39)	K233N	probably damaging	Het
Nr2c2ap	G	A	8: 70,584,653 (GRCm39)	D42N	probably damaging	Het
Ntn1	A	G	11: 68,276,538 (GRCm39)	S137P	probably benign	Het
Pard3	T	C	8: 128,186,867 (GRCm39)		probably null	Het
Pcdh15	T	A	10: 74,430,015 (GRCm39)	D1210E	probably damaging	Het
Pcdhga5	T	C	18: 37,827,774 (GRCm39)	L74P	probably benign	Het
Pcsk5	T	C	19: 17,573,022 (GRCm39)		probably null	Het
Pdia3	T	C	2: 121,244,484 (GRCm39)		probably benign	Het
Pikfyve	A	T	1: 65,235,858 (GRCm39)	R177*	probably null	Het
Pklr	A	G	3: 89,050,566 (GRCm39)	E409G	probably damaging	Het
Plod2	T	C	9: 92,463,376 (GRCm39)	Y154H	probably damaging	Het
Psmc3	T	A	2: 90,884,667 (GRCm39)	D6E	probably benign	Het
Ptpru	T	A	4: 131,547,334 (GRCm39)	N205I	possibly damaging	Het
Rbm6	T	C	9: 107,655,213 (GRCm39)	E1006G	probably benign	Het
Rgl2	T	C	17: 34,154,922 (GRCm39)	V642A	probably benign	Het
Rnf8	T	A	17: 29,845,680 (GRCm39)	H104Q	possibly damaging	Het
Robo4	CGG	CG	9: 37,322,786 (GRCm39)		probably null	Het
Rpa1	T	C	11: 75,204,170 (GRCm39)	N269S	probably damaging	Het
Sdk2	A	G	11: 113,757,857 (GRCm39)	M519T	probably benign	Het
Snx30	A	G	4: 59,885,070 (GRCm39)	S237G	probably benign	Het
Spx	A	G	6: 142,359,766 (GRCm39)	N36S	probably damaging	Het
Stard7	T	C	2: 127,137,416 (GRCm39)	Y289H	probably damaging	Het
Sugp2	T	C	8: 70,709,757 (GRCm39)		probably benign	Het
Susd4	C	T	1: 182,715,043 (GRCm39)	T288I	probably damaging	Het
Tceanc2	T	A	4: 107,034,826 (GRCm39)		probably null	Het
Tm4sf4	T	G	3: 57,341,159 (GRCm39)	V97G	probably benign	Het
Tmtc1	C	T	6: 148,256,629 (GRCm39)		probably benign	Het
Trappc11	C	T	8: 47,958,339 (GRCm39)		probably benign	Het
Triobp	G	T	15: 78,878,591 (GRCm39)	V398F	possibly damaging	Het
Ttll9	T	A	2: 152,804,464 (GRCm39)	S2T	possibly damaging	Het
Ttn	T	C	2: 76,731,320 (GRCm39)		probably benign	Het
Tyw3	A	G	3: 154,300,108 (GRCm39)	C80R	probably damaging	Het
Usp24	T	C	4: 106,242,621 (GRCm39)	V1177A	possibly damaging	Het
Vmn2r104	T	A	17: 20,262,146 (GRCm39)	H328L	probably benign	Het
Vmn2r65	A	C	7: 84,589,849 (GRCm39)	I689S	probably damaging	Het
Wrn	A	G	8: 33,731,129 (GRCm39)	Y1068H	probably damaging	Het
Xpo4	C	A	14: 57,850,866 (GRCm39)	S346I	probably benign	Het
Zc3h3	T	A	15: 75,711,439 (GRCm39)	T341S	probably benign	Het
Zxdc	T	C	6: 90,347,419 (GRCm39)	M260T	possibly damaging	Het

Other mutations in Vmn1r65

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01636:Vmn1r65	APN	7	6,011,720 (GRCm39)	missense	probably benign	0.12
IGL02821:Vmn1r65	APN	7	6,011,893 (GRCm39)	missense	possibly damaging	0.62
R0991:Vmn1r65	UTSW	7	6,012,076 (GRCm39)	missense	probably damaging	0.99
R1457:Vmn1r65	UTSW	7	6,012,156 (GRCm39)	missense	probably benign	0.10
R1507:Vmn1r65	UTSW	7	6,012,108 (GRCm39)	missense	probably benign	0.13
R1856:Vmn1r65	UTSW	7	6,011,265 (GRCm39)	missense	possibly damaging	0.93
R2914:Vmn1r65	UTSW	7	6,012,040 (GRCm39)	missense	possibly damaging	0.69
R5147:Vmn1r65	UTSW	7	6,011,818 (GRCm39)	missense	probably benign	0.01
R5399:Vmn1r65	UTSW	7	6,011,809 (GRCm39)	nonsense	probably null
R5409:Vmn1r65	UTSW	7	6,012,012 (GRCm39)	missense	possibly damaging	0.77
R5646:Vmn1r65	UTSW	7	6,012,223 (GRCm39)	missense	probably benign	0.01
R5963:Vmn1r65	UTSW	7	6,011,608 (GRCm39)	missense	probably damaging	1.00
R6034:Vmn1r65	UTSW	7	6,011,868 (GRCm39)	missense	probably damaging	1.00
R6034:Vmn1r65	UTSW	7	6,011,868 (GRCm39)	missense	probably damaging	1.00
R6327:Vmn1r65	UTSW	7	6,011,651 (GRCm39)	missense	possibly damaging	0.48
R7958:Vmn1r65	UTSW	7	6,011,254 (GRCm39)	missense	probably benign	0.01
R8121:Vmn1r65	UTSW	7	6,011,464 (GRCm39)	missense	possibly damaging	0.87
R8345:Vmn1r65	UTSW	7	6,011,256 (GRCm39)	missense	probably benign
R8725:Vmn1r65	UTSW	7	6,011,503 (GRCm39)	missense	probably damaging	1.00
R8727:Vmn1r65	UTSW	7	6,011,503 (GRCm39)	missense	probably damaging	1.00
R9246:Vmn1r65	UTSW	7	6,011,769 (GRCm39)	missense	possibly damaging	0.95
R9279:Vmn1r65	UTSW	7	6,011,988 (GRCm39)	missense	probably benign	0.06
R9778:Vmn1r65	UTSW	7	6,011,387 (GRCm39)	nonsense	probably null

Predicted Primers

PCR Primer
(F):5'- GTTCAGGTGAAGCACTATGGAGAC -3'
(R):5'- TCCAAGGAAGCCTCCAACTG -3'

Sequencing Primer
(F):5'- TGGAGACACTGGTCCAGATC -3'
(R):5'- ACTGACCTCAAATGTAAACTTGC -3'

Posted On

2016-07-22