Incidental Mutation 'R5292:Zfp936'
| ID |
405200 |
| Institutional Source |
Beutler Lab
|
| Gene Symbol |
Zfp936
|
| Ensembl Gene |
ENSMUSG00000064194 |
| Gene Name |
zinc finger protein 936 |
| Synonyms |
EG435970, Gm9272, I1C0022H11Rik |
| MMRRC Submission |
042875-MU
|
| Accession Numbers |
|
| Essential gene? |
Possibly essential
(E-score: 0.686)
|
| Stock # |
R5292 (G1)
|
| Quality Score |
97 |
|
Status
|
Not validated
|
| Chromosome |
7 |
| Chromosomal Location |
42763653-42841533 bp(+) (GRCm39) |
| Type of Mutation |
nonsense |
| DNA Base Change (assembly) |
T to A
at 42838759 bp (GRCm39)
|
| Zygosity |
Heterozygous |
| Amino Acid Change |
Tyrosine to Stop codon
at position 75
(Y75*)
|
| Ref Sequence |
ENSEMBL: ENSMUSP00000145999
(fasta)
|
| Gene Model |
predicted gene model for transcript(s):
[ENSMUST00000072829]
[ENSMUST00000200973]
[ENSMUST00000202535]
[ENSMUST00000205912]
|
| AlphaFold |
Q3ULA8 |
| Predicted Effect |
probably null
Transcript: ENSMUST00000072829
AA Change: Y74*
|
| SMART Domains |
Protein: ENSMUSP00000072608
Gene: ENSMUSG00000064194
AA Change: Y74*
| Domain | Start | End | E-Value | Type |
|---|
|
KRAB
|
3 |
65 |
5.32e-19 |
SMART |
|
ZnF_C2H2
|
148 |
170 |
5.9e-3 |
SMART |
|
ZnF_C2H2
|
176 |
198 |
5.9e-3 |
SMART |
|
ZnF_C2H2
|
204 |
226 |
1.4e-4 |
SMART |
|
ZnF_C2H2
|
232 |
254 |
1.2e-3 |
SMART |
|
ZnF_C2H2
|
260 |
282 |
7.37e-4 |
SMART |
|
ZnF_C2H2
|
288 |
310 |
2.27e-4 |
SMART |
|
ZnF_C2H2
|
316 |
338 |
2.09e-3 |
SMART |
|
ZnF_C2H2
|
344 |
366 |
1.45e-2 |
SMART |
|
ZnF_C2H2
|
372 |
394 |
1.82e-3 |
SMART |
|
ZnF_C2H2
|
400 |
422 |
2.53e-2 |
SMART |
|
ZnF_C2H2
|
428 |
450 |
4.54e-4 |
SMART |
|
| Predicted Effect |
probably benign
Transcript: ENSMUST00000200973
|
| SMART Domains |
Protein: ENSMUSP00000144191
Gene: ENSMUSG00000064194
| Domain | Start | End | E-Value | Type |
|---|
|
KRAB
|
4 |
66 |
1.4e-21 |
SMART |
|
| Predicted Effect |
probably null
Transcript: ENSMUST00000202535
AA Change: Y75*
|
| SMART Domains |
Protein: ENSMUSP00000143800
Gene: ENSMUSG00000064194
AA Change: Y75*
| Domain | Start | End | E-Value | Type |
|---|
|
KRAB
|
4 |
66 |
2.3e-21 |
SMART |
|
ZnF_C2H2
|
149 |
171 |
2.6e-5 |
SMART |
|
ZnF_C2H2
|
177 |
199 |
2.5e-5 |
SMART |
|
ZnF_C2H2
|
205 |
227 |
5.9e-7 |
SMART |
|
ZnF_C2H2
|
233 |
255 |
5.1e-6 |
SMART |
|
ZnF_C2H2
|
261 |
283 |
3.1e-6 |
SMART |
|
ZnF_C2H2
|
289 |
311 |
9.4e-7 |
SMART |
|
ZnF_C2H2
|
317 |
339 |
8.7e-6 |
SMART |
|
ZnF_C2H2
|
345 |
367 |
6.3e-5 |
SMART |
|
ZnF_C2H2
|
373 |
395 |
7.7e-6 |
SMART |
|
ZnF_C2H2
|
401 |
423 |
1.1e-4 |
SMART |
|
ZnF_C2H2
|
429 |
451 |
2e-6 |
SMART |
|
| Predicted Effect |
probably null
Transcript: ENSMUST00000205912
AA Change: Y75*
|
| Coding Region Coverage |
- 1x: 99.3%
- 3x: 98.7%
- 10x: 97.4%
- 20x: 95.6%
|
| Validation Efficiency |
|
| Allele List at MGI |
|
| Other mutations in this stock |
Total: 47 list
| Gene | Ref | Var | Chr/Loc | Mutation | Predicted Effect | Zygosity |
|---|
| Adam32 |
A |
G |
8: 25,354,467 (GRCm39) |
V641A |
possibly damaging |
Het |
| Apob |
A |
T |
12: 8,055,912 (GRCm39) |
M1465L |
probably benign |
Het |
| Astn1 |
T |
C |
1: 158,407,933 (GRCm39) |
|
probably null |
Het |
| Bend7 |
G |
A |
2: 4,768,052 (GRCm39) |
R336Q |
probably damaging |
Het |
| Col22a1 |
T |
C |
15: 71,842,185 (GRCm39) |
Y433C |
probably damaging |
Het |
| Crtc3 |
T |
C |
7: 80,268,358 (GRCm39) |
T154A |
possibly damaging |
Het |
| Dcc |
T |
C |
18: 71,439,159 (GRCm39) |
Y1241C |
probably damaging |
Het |
| Depdc1b |
T |
C |
13: 108,510,376 (GRCm39) |
V296A |
probably damaging |
Het |
| Gata3 |
A |
G |
2: 9,873,685 (GRCm39) |
S270P |
probably damaging |
Het |
| Gcm1 |
T |
C |
9: 77,968,708 (GRCm39) |
F136S |
probably damaging |
Het |
| Gfpt1 |
A |
G |
6: 87,053,237 (GRCm39) |
|
probably null |
Het |
| Gm5174 |
G |
T |
10: 86,492,562 (GRCm39) |
|
noncoding transcript |
Het |
| Hhipl1 |
A |
G |
12: 108,294,037 (GRCm39) |
T648A |
probably benign |
Het |
| Hr |
C |
A |
14: 70,809,432 (GRCm39) |
Q1177K |
probably damaging |
Het |
| Hrnr |
T |
C |
3: 93,239,199 (GRCm39) |
S3146P |
unknown |
Het |
| Igkv10-94 |
C |
T |
6: 68,681,582 (GRCm39) |
G86E |
probably damaging |
Het |
| Krt80 |
G |
A |
15: 101,250,066 (GRCm39) |
R222W |
probably damaging |
Het |
| Lrch3 |
T |
C |
16: 32,796,177 (GRCm39) |
Y354H |
probably damaging |
Het |
| Or10g1 |
A |
T |
14: 52,647,902 (GRCm39) |
N142K |
possibly damaging |
Het |
| Or3a1b |
T |
A |
11: 74,012,877 (GRCm39) |
F254Y |
probably damaging |
Het |
| Or5w1b |
T |
A |
2: 87,476,339 (GRCm39) |
N43Y |
probably damaging |
Het |
| Osbpl7 |
A |
G |
11: 96,958,779 (GRCm39) |
D932G |
probably benign |
Het |
| Pakap |
T |
G |
4: 57,855,356 (GRCm39) |
S471R |
probably damaging |
Het |
| Palmd |
T |
C |
3: 116,717,393 (GRCm39) |
E368G |
probably benign |
Het |
| Peg3 |
T |
A |
7: 6,711,259 (GRCm39) |
D1321V |
probably damaging |
Het |
| Pkhd1l1 |
A |
T |
15: 44,392,962 (GRCm39) |
I1766F |
probably damaging |
Het |
| Polq |
A |
G |
16: 36,881,745 (GRCm39) |
E1303G |
probably damaging |
Het |
| Ppie |
A |
G |
4: 123,033,701 (GRCm39) |
Y9H |
probably damaging |
Het |
| Ptprz1 |
A |
G |
6: 23,002,581 (GRCm39) |
N1557S |
probably benign |
Het |
| Rnf40 |
T |
C |
7: 127,195,120 (GRCm39) |
V411A |
possibly damaging |
Het |
| Rtn4 |
T |
C |
11: 29,657,924 (GRCm39) |
F577L |
probably benign |
Het |
| Sacs |
A |
G |
14: 61,449,432 (GRCm39) |
Y3826C |
probably damaging |
Het |
| Slc32a1 |
A |
G |
2: 158,453,307 (GRCm39) |
D49G |
probably damaging |
Het |
| Slc37a2 |
A |
T |
9: 37,150,453 (GRCm39) |
C167* |
probably null |
Het |
| Slc5a1 |
G |
T |
5: 33,315,585 (GRCm39) |
V535F |
probably benign |
Het |
| Smoc2 |
A |
G |
17: 14,556,835 (GRCm39) |
K95E |
probably damaging |
Het |
| Smok3c |
A |
C |
5: 138,063,446 (GRCm39) |
Q311P |
probably damaging |
Het |
| Spink5 |
C |
A |
18: 44,139,521 (GRCm39) |
P628Q |
probably benign |
Het |
| Spint4 |
T |
C |
2: 164,542,779 (GRCm39) |
L124S |
probably benign |
Het |
| Stard9 |
A |
G |
2: 120,529,626 (GRCm39) |
K1961R |
probably benign |
Het |
| Synpo2 |
C |
A |
3: 122,907,709 (GRCm39) |
V536L |
possibly damaging |
Het |
| Trim24 |
A |
G |
6: 37,880,539 (GRCm39) |
K146E |
probably benign |
Het |
| Usp24 |
A |
G |
4: 106,275,460 (GRCm39) |
D2245G |
probably benign |
Het |
| Vars2 |
A |
G |
17: 35,971,678 (GRCm39) |
S81P |
probably damaging |
Het |
| Zfp39 |
T |
C |
11: 58,791,415 (GRCm39) |
T91A |
probably damaging |
Het |
| Zfp764l1 |
T |
C |
7: 126,991,659 (GRCm39) |
D97G |
possibly damaging |
Het |
| Zkscan5 |
G |
T |
5: 145,155,451 (GRCm39) |
C374F |
probably damaging |
Het |
|
| Other mutations in Zfp936 |
| Allele | Source | Chr | Coord | Type | Predicted Effect | PPH Score |
|---|
|
IGL02231:Zfp936
|
APN |
7 |
42,836,909 (GRCm39) |
splice site |
probably null |
|
|
IGL02245:Zfp936
|
APN |
7 |
42,836,722 (GRCm39) |
critical splice donor site |
probably null |
|
|
IGL02335:Zfp936
|
APN |
7 |
42,836,691 (GRCm39) |
missense |
probably damaging |
1.00 |
|
FR4340:Zfp936
|
UTSW |
7 |
42,838,913 (GRCm39) |
missense |
possibly damaging |
0.63 |
|
R0437:Zfp936
|
UTSW |
7 |
42,838,734 (GRCm39) |
missense |
probably benign |
0.00 |
|
R3899:Zfp936
|
UTSW |
7 |
42,839,158 (GRCm39) |
missense |
possibly damaging |
0.93 |
|
R4120:Zfp936
|
UTSW |
7 |
42,839,630 (GRCm39) |
missense |
probably benign |
0.10 |
|
R4406:Zfp936
|
UTSW |
7 |
42,839,748 (GRCm39) |
missense |
possibly damaging |
0.82 |
|
R4959:Zfp936
|
UTSW |
7 |
42,839,034 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R5023:Zfp936
|
UTSW |
7 |
42,836,681 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R5163:Zfp936
|
UTSW |
7 |
42,839,664 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R5182:Zfp936
|
UTSW |
7 |
42,839,331 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R5668:Zfp936
|
UTSW |
7 |
42,839,858 (GRCm39) |
missense |
possibly damaging |
0.93 |
|
R6057:Zfp936
|
UTSW |
7 |
42,839,787 (GRCm39) |
missense |
probably benign |
0.00 |
|
R6901:Zfp936
|
UTSW |
7 |
42,839,467 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R7139:Zfp936
|
UTSW |
7 |
42,839,715 (GRCm39) |
missense |
possibly damaging |
0.54 |
|
R7258:Zfp936
|
UTSW |
7 |
42,839,803 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R7440:Zfp936
|
UTSW |
7 |
42,836,685 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R7537:Zfp936
|
UTSW |
7 |
42,839,239 (GRCm39) |
nonsense |
probably null |
|
|
R7561:Zfp936
|
UTSW |
7 |
42,839,339 (GRCm39) |
missense |
probably damaging |
0.98 |
|
R7662:Zfp936
|
UTSW |
7 |
42,839,336 (GRCm39) |
nonsense |
probably null |
|
|
R7775:Zfp936
|
UTSW |
7 |
42,839,720 (GRCm39) |
missense |
possibly damaging |
0.70 |
|
R7778:Zfp936
|
UTSW |
7 |
42,839,720 (GRCm39) |
missense |
possibly damaging |
0.70 |
|
R8016:Zfp936
|
UTSW |
7 |
42,838,848 (GRCm39) |
missense |
possibly damaging |
0.61 |
|
R8121:Zfp936
|
UTSW |
7 |
42,839,547 (GRCm39) |
missense |
possibly damaging |
0.55 |
|
R9012:Zfp936
|
UTSW |
7 |
42,839,416 (GRCm39) |
nonsense |
probably null |
|
|
R9058:Zfp936
|
UTSW |
7 |
42,839,196 (GRCm39) |
missense |
probably benign |
0.32 |
|
R9188:Zfp936
|
UTSW |
7 |
42,839,768 (GRCm39) |
missense |
probably benign |
0.00 |
|
R9236:Zfp936
|
UTSW |
7 |
42,836,922 (GRCm39) |
missense |
probably benign |
0.00 |
|
R9596:Zfp936
|
UTSW |
7 |
42,839,834 (GRCm39) |
missense |
probably damaging |
1.00 |
|
| Predicted Primers |
PCR Primer
(F):5'- TGGTGTAAATGTGAGCCAAGGA -3'
(R):5'- GTTTGTAGGGTTTCTCTCCAGTA -3'
Sequencing Primer
(F):5'- TGGTTGAGAGCCACCATATG -3'
(R):5'- AGGGTTTCTCTCCAGTATTTGTTC -3'
|
| Posted On |
2016-07-22 |
Incidental Mutation