Mutagenetix > Incidental Mutation

Incidental Mutation 'R5300:Zfp772'

405600

Institutional Source

Beutler Lab

Gene Symbol

Zfp772

Ensembl Gene

ENSMUSG00000066838

Gene Name

zinc finger protein 772

Synonyms

MMRRC Submission

042883-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.068) question?

Stock #

R5300 (G1)

Quality Score

117

Status

Not validated

Chromosome

Chromosomal Location

7205121-7212997 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to T at 7207157 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Methionine to Lysine at position 178 (M178K)

Ref Sequence

ENSEMBL: ENSMUSP00000074055 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000074455]

AlphaFold

Q3UQL6

Predicted Effect

probably benign
Transcript: ENSMUST00000074455
AA Change: M178K

PolyPhen 2 Score 0.000 (Sensitivity: 1.00; Specificity: 0.00)

SMART Domains

Protein: ENSMUSP00000074055
Gene: ENSMUSG00000066838
AA Change: M178K

Domain	Start	End	E-Value	Type
KRAB	42	102	1.87e-14	SMART
ZnF_C2H2	193	215	1.36e-2	SMART
ZnF_C2H2	221	243	5.21e-4	SMART
ZnF_C2H2	249	271	1.38e-3	SMART
ZnF_C2H2	277	299	4.24e-4	SMART
ZnF_C2H2	305	327	5.21e-4	SMART
ZnF_C2H2	333	355	2.27e-4	SMART
ZnF_C2H2	361	383	1.18e-2	SMART
ZnF_C2H2	389	411	4.87e-4	SMART

Coding Region Coverage

1x: 99.2%
3x: 98.5%
10x: 96.6%
20x: 93.1%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 28 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Ank1	A	G	8: 23,622,517 (GRCm39)	K1644E	probably benign	Het
C7	C	A	15: 5,061,432 (GRCm39)	G306W	probably damaging	Het
Ccdc185	A	G	1: 182,575,645 (GRCm39)	V348A	probably benign	Het
Cfap58	A	T	19: 47,929,595 (GRCm39)	I19F	probably benign	Het
Dnaaf2	C	G	12: 69,245,002 (GRCm39)	V20L	probably damaging	Het
Dpy19l3	A	G	7: 35,426,735 (GRCm39)	Y58H	probably damaging	Het
Dsc1	T	C	18: 20,227,917 (GRCm39)	S480G	probably damaging	Het
Eno4	G	A	19: 58,943,982 (GRCm39)		probably null	Het
Gm136	T	G	4: 34,750,930 (GRCm39)	K114N	probably damaging	Het
Hjurp	GT	GTT	1: 88,194,246 (GRCm39)		probably null	Het
Lgr6	C	T	1: 134,921,748 (GRCm39)	A199T	probably damaging	Het
Lrrc37a	T	A	11: 103,347,784 (GRCm39)	L2970F	unknown	Het
Ly86	C	T	13: 37,602,588 (GRCm39)	T160I	probably benign	Het
Mfsd4b1	A	C	10: 39,879,027 (GRCm39)	F290C	probably benign	Het
Minar1	A	T	9: 89,485,252 (GRCm39)	S48R	probably damaging	Het
Nbeal1	T	G	1: 60,274,718 (GRCm39)	Y376*	probably null	Het
Nipbl	A	T	15: 8,380,981 (GRCm39)	S604T	probably benign	Het
Pml	A	C	9: 58,154,302 (GRCm39)	N190K	probably damaging	Het
Rin3	C	A	12: 102,335,929 (GRCm39)	F533L	probably benign	Het
Scamp1	A	G	13: 94,340,670 (GRCm39)	V233A	probably damaging	Het
Sppl2c	A	T	11: 104,077,901 (GRCm39)	N234Y	possibly damaging	Het
Tfap2b	A	G	1: 19,298,677 (GRCm39)	K278R	probably damaging	Het
Tg	A	G	15: 66,550,704 (GRCm39)	Y278C	probably damaging	Het
Trdn	A	G	10: 33,071,978 (GRCm39)	E215G	probably damaging	Het
Tssk6	T	C	8: 70,355,285 (GRCm39)	S110P	probably benign	Het
Vmn2r15	A	T	5: 109,441,974 (GRCm39)	L153Q	probably damaging	Het
Wdr17	C	T	8: 55,134,434 (GRCm39)	G349R	probably damaging	Het
Zfp667	A	G	7: 6,307,635 (GRCm39)	H101R	probably benign	Het

Other mutations in Zfp772

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00480:Zfp772	APN	7	7,207,115 (GRCm39)	missense	probably benign
IGL01589:Zfp772	APN	7	7,208,523 (GRCm39)	missense	possibly damaging	0.53
PIT4449001:Zfp772	UTSW	7	7,207,350 (GRCm39)	missense	probably benign	0.03
R1945:Zfp772	UTSW	7	7,206,629 (GRCm39)	missense	probably benign	0.01
R3085:Zfp772	UTSW	7	7,206,699 (GRCm39)	missense	possibly damaging	0.53
R5793:Zfp772	UTSW	7	7,207,283 (GRCm39)	missense	probably benign	0.00
R6252:Zfp772	UTSW	7	7,207,018 (GRCm39)	missense	possibly damaging	0.86
R6605:Zfp772	UTSW	7	7,208,547 (GRCm39)	missense	possibly damaging	0.72
R6751:Zfp772	UTSW	7	7,206,716 (GRCm39)	missense	possibly damaging	0.90
R6812:Zfp772	UTSW	7	7,209,307 (GRCm39)	missense	possibly damaging	0.92
R8171:Zfp772	UTSW	7	7,207,096 (GRCm39)	nonsense	probably null
R8696:Zfp772	UTSW	7	7,208,518 (GRCm39)	missense	possibly damaging	0.53
R9104:Zfp772	UTSW	7	7,207,190 (GRCm39)	missense	possibly damaging	0.53
R9665:Zfp772	UTSW	7	7,212,793 (GRCm39)	start codon destroyed	probably null

Predicted Primers

PCR Primer
(F):5'- TCTCCGGTGTGAATTCTGAAATGC -3'
(R):5'- GTTGTTGGATCAAAGTGGACAG -3'

Sequencing Primer
(F):5'- GCTTAATAAGAAAGGCTTTTTGGC -3'
(R):5'- TTGGATCAAAGTGGACAGTGAAATG -3'

Posted On

2016-07-22