Mutagenetix > Incidental Mutation

Incidental Mutation 'R5314:Or8g32'

405750

Institutional Source

Beutler Lab

Gene Symbol

Or8g32

Ensembl Gene

ENSMUSG00000094269

Gene Name

olfactory receptor family 8 subfamily G member 32

Synonyms

GA_x6K02T2PVTD-33090395-33091330, MOR171-33P, MOR171-49, Olfr951

MMRRC Submission

042897-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.081) question?

Stock #

R5314 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

39305089-39306033 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 39305785 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Serine to Proline at position 233 (S233P)

Ref Sequence

ENSEMBL: ENSMUSP00000077615 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000078531] [ENSMUST00000216107]

AlphaFold

Q9EQ94

Predicted Effect

probably damaging
Transcript: ENSMUST00000078531
AA Change: S233P

PolyPhen 2 Score 0.997 (Sensitivity: 0.41; Specificity: 0.98)

SMART Domains

Protein: ENSMUSP00000077615
Gene: ENSMUSG00000094269
AA Change: S233P

Domain	Start	End	E-Value	Type
Pfam:7tm_4	34	311	2.5e-52	PFAM
Pfam:7tm_1	44	293	5e-19	PFAM

Predicted Effect

probably damaging
Transcript: ENSMUST00000216107
AA Change: S230P

PolyPhen 2 Score 0.996 (Sensitivity: 0.55; Specificity: 0.98)

Coding Region Coverage

1x: 99.2%
3x: 98.6%
10x: 97.1%
20x: 94.7%

Validation Efficiency

MGI Phenotype

FUNCTION: Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]

Allele List at MGI

Other mutations in this stock

Total: 54 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
4930432M17Rik	T	C	3: 121,473,172 (GRCm39)	F109S	unknown	Het
Ankhd1	A	T	18: 36,694,111 (GRCm39)		probably null	Het
Atp8a1	C	T	5: 67,863,248 (GRCm39)		probably null	Het
Ccdc136	G	T	6: 29,417,497 (GRCm39)	V707F	probably benign	Het
Ccdc157	A	T	11: 4,100,078 (GRCm39)	C91*	probably null	Het
Ceacam3	T	A	7: 16,892,296 (GRCm39)	N346K	possibly damaging	Het
Cfap251	A	G	5: 123,460,626 (GRCm39)	D1196G	probably benign	Het
Chd4	A	G	6: 125,077,551 (GRCm39)	E74G	probably damaging	Het
Cntn1	A	G	15: 92,192,892 (GRCm39)	M665V	probably benign	Het
Crtc2	C	T	3: 90,168,348 (GRCm39)	Q364*	probably null	Het
Csf1r	T	A	18: 61,262,796 (GRCm39)	I857N	probably damaging	Het
Dner	C	T	1: 84,558,460 (GRCm39)	G168D	probably damaging	Het
Edar	T	C	10: 58,443,182 (GRCm39)	T315A	probably benign	Het
Egflam	A	G	15: 7,333,493 (GRCm39)	V153A	probably damaging	Het
Enoph1	T	C	5: 100,211,682 (GRCm39)	I193T	possibly damaging	Het
Epc1	A	G	18: 6,462,969 (GRCm39)	I9T	probably damaging	Het
Fbxw18	T	A	9: 109,522,246 (GRCm39)	I208F	possibly damaging	Het
Gm4841	A	G	18: 60,403,364 (GRCm39)	V243A	probably benign	Het
Herc2	T	C	7: 55,869,534 (GRCm39)	V4297A	probably damaging	Het
Itsn2	C	T	12: 4,677,960 (GRCm39)	P106S	probably benign	Het
Kcnu1	A	G	8: 26,352,486 (GRCm39)	T218A	probably damaging	Het
Kif1a	A	G	1: 92,946,220 (GRCm39)	S1677P	probably damaging	Het
Krt14	A	T	11: 100,095,526 (GRCm39)	M293K	probably damaging	Het
Meis3	T	A	7: 15,917,989 (GRCm39)	V307E	probably damaging	Het
Nadk2	A	C	15: 9,108,401 (GRCm39)	I417L	probably benign	Het
Nav2	AAGCAGCAGCAGCAGCAGCAGCAGCA	AAGCAGCAGCAGCAGCAGCAGCA	7: 49,058,440 (GRCm39)		probably benign	Het
Neb	A	T	2: 52,171,515 (GRCm39)	N1659K	probably benign	Het
Nhsl3	T	C	4: 129,118,005 (GRCm39)	T208A	possibly damaging	Het
Or10w1	T	C	19: 13,632,630 (GRCm39)	V274A	probably benign	Het
Or8s5	G	A	15: 98,238,246 (GRCm39)	A208V	probably benign	Het
Pde3b	A	T	7: 114,093,772 (GRCm39)	N339Y	probably damaging	Het
Pde8b	A	G	13: 95,223,361 (GRCm39)	F298L	possibly damaging	Het
Phtf1	G	A	3: 103,906,603 (GRCm39)	R606H	probably damaging	Het
Psd4	A	G	2: 24,290,528 (GRCm39)	D535G	possibly damaging	Het
Rad51ap1	A	G	6: 126,905,121 (GRCm39)	V130A	probably damaging	Het
Rbm11	T	C	16: 75,393,474 (GRCm39)	F57L	probably damaging	Het
Rprd2	A	G	3: 95,671,401 (GRCm39)	V1334A	possibly damaging	Het
Satb2	T	C	1: 56,870,686 (GRCm39)	E433G	probably damaging	Het
Sema6b	G	A	17: 56,435,413 (GRCm39)	R277*	probably null	Het
Sepsecs	A	T	5: 52,805,015 (GRCm39)	S349T	probably benign	Het
Slc35b2	T	C	17: 45,877,424 (GRCm39)	Y184H	probably damaging	Het
Slc7a2	T	C	8: 41,368,067 (GRCm39)		probably null	Het
Smc1b	T	A	15: 84,955,066 (GRCm39)	Y1062F	probably benign	Het
Snrnp70	G	A	7: 45,026,476 (GRCm39)	R298*	probably null	Het
Sntb1	C	G	15: 55,506,191 (GRCm39)	G461R	probably damaging	Het
Taar8c	A	T	10: 23,977,246 (GRCm39)	C189S	probably damaging	Het
Tas1r2	T	A	4: 139,382,672 (GRCm39)	D103E	probably damaging	Het
Timd2	T	C	11: 46,568,087 (GRCm39)	I236V	probably benign	Het
Tmem87a	A	G	2: 120,208,407 (GRCm39)	V316A	probably damaging	Het
Treml2	T	A	17: 48,607,601 (GRCm39)	L16Q	probably damaging	Het
Zcchc14	G	A	8: 122,335,337 (GRCm39)		probably benign	Het
Zfp462	T	C	4: 55,013,178 (GRCm39)	Y567H	probably damaging	Het
Zfp551	G	A	7: 12,150,087 (GRCm39)	R441*	probably null	Het
Zfp930	A	G	8: 69,679,373 (GRCm39)	I59M	probably benign	Het

Other mutations in Or8g32

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01527:Or8g32	APN	9	39,305,114 (GRCm39)	missense	probably benign	0.01
IGL01650:Or8g32	APN	9	39,305,252 (GRCm39)	missense	probably damaging	0.99
IGL02134:Or8g32	APN	9	39,305,830 (GRCm39)	missense	probably damaging	0.99
IGL03113:Or8g32	APN	9	39,305,981 (GRCm39)	missense	probably damaging	1.00
R0127:Or8g32	UTSW	9	39,305,238 (GRCm39)	missense	probably benign	0.16
R1730:Or8g32	UTSW	9	39,305,518 (GRCm39)	missense	probably benign	0.01
R1783:Or8g32	UTSW	9	39,305,518 (GRCm39)	missense	probably benign	0.01
R1924:Or8g32	UTSW	9	39,305,163 (GRCm39)	missense	possibly damaging	0.93
R3785:Or8g32	UTSW	9	39,305,678 (GRCm39)	missense	probably benign	0.07
R3787:Or8g32	UTSW	9	39,305,678 (GRCm39)	missense	probably benign	0.07
R4607:Or8g32	UTSW	9	39,306,031 (GRCm39)	makesense	probably null
R4803:Or8g32	UTSW	9	39,305,932 (GRCm39)	missense	probably benign	0.26
R5338:Or8g32	UTSW	9	39,305,371 (GRCm39)	missense	probably damaging	1.00
R5360:Or8g32	UTSW	9	39,305,698 (GRCm39)	missense	probably benign	0.00
R5468:Or8g32	UTSW	9	39,305,257 (GRCm39)	missense	probably benign	0.33
R6590:Or8g32	UTSW	9	39,305,845 (GRCm39)	missense	probably benign	0.00
R6690:Or8g32	UTSW	9	39,305,845 (GRCm39)	missense	probably benign	0.00
R6925:Or8g32	UTSW	9	39,305,157 (GRCm39)	missense	probably benign	0.01
R6925:Or8g32	UTSW	9	39,305,156 (GRCm39)	missense	probably benign	0.32
R6982:Or8g32	UTSW	9	39,305,618 (GRCm39)	missense	probably damaging	1.00
R7662:Or8g32	UTSW	9	39,305,389 (GRCm39)	missense	probably benign	0.01
R8074:Or8g32	UTSW	9	39,305,242 (GRCm39)	missense	probably damaging	1.00
R8389:Or8g32	UTSW	9	39,305,912 (GRCm39)	missense	probably damaging	1.00
R9444:Or8g32	UTSW	9	39,305,365 (GRCm39)	missense	probably benign	0.19
R9642:Or8g32	UTSW	9	39,305,857 (GRCm39)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- GGCTTCATGTTTAGGATCCAGTTC -3'
(R):5'- TCCTCAGGCTGTAGATCAAGGG -3'

Sequencing Primer
(F):5'- AGGATCCAGTTCTGCAAATCAG -3'
(R):5'- CTGTAGATCAAGGGGTTCAGC -3'

Posted On

2016-07-22