Mutagenetix > Incidental Mutation

Incidental Mutation 'R5319:Or7g25'

405990

Institutional Source

Beutler Lab

Gene Symbol

Or7g25

Ensembl Gene

ENSMUSG00000048391

Gene Name

olfactory receptor family 7 subfamily G member 25

Synonyms

GA_x6K02T2PVTD-12986331-12985390, Olfr843, MOR155-2

MMRRC Submission

042902-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.130) question?

Stock #

R5319 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

19159653-19160759 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

C to T at 19160329 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Arginine to Histidine at position 122 (R122H)

Ref Sequence

ENSEMBL: ENSMUSP00000151203 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000061365] [ENSMUST00000214019] [ENSMUST00000214267]

AlphaFold

Q7TRG4

Predicted Effect

possibly damaging
Transcript: ENSMUST00000061365
AA Change: R122H

PolyPhen 2 Score 0.602 (Sensitivity: 0.87; Specificity: 0.91)

SMART Domains

Protein: ENSMUSP00000051027
Gene: ENSMUSG00000048391
AA Change: R122H

Domain	Start	End	E-Value	Type
Pfam:7tm_4	31	307	2.4e-51	PFAM
Pfam:7tm_1	41	290	3.3e-24	PFAM

Predicted Effect

possibly damaging
Transcript: ENSMUST00000214019
AA Change: R122H

PolyPhen 2 Score 0.602 (Sensitivity: 0.87; Specificity: 0.91)

Predicted Effect

possibly damaging
Transcript: ENSMUST00000214267
AA Change: R122H

PolyPhen 2 Score 0.602 (Sensitivity: 0.87; Specificity: 0.91)

Meta Mutation Damage Score

0.1024

Coding Region Coverage

1x: 99.3%
3x: 98.7%
10x: 97.5%
20x: 95.8%

Validation Efficiency

95% (69/73)

MGI Phenotype

FUNCTION: Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]

Allele List at MGI

Other mutations in this stock

Total: 62 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
4930449I24Rik	T	C	5: 146,441,506 (GRCm39)	S218P	probably benign	Het
Ahctf1	A	C	1: 179,596,615 (GRCm39)	S121A	probably damaging	Het
Anpep	C	T	7: 79,491,479 (GRCm39)	R174H	probably benign	Het
Arhgef18	T	C	8: 3,435,010 (GRCm39)		probably null	Het
Becn1	A	G	11: 101,179,629 (GRCm39)		probably benign	Het
Cabin1	T	C	10: 75,561,549 (GRCm39)	Y984C	probably damaging	Het
Cacna2d4	G	T	6: 119,324,213 (GRCm39)		probably null	Het
Ccdc14	T	G	16: 34,543,542 (GRCm39)	N633K	probably damaging	Het
Cdk5rap1	T	A	2: 154,177,489 (GRCm39)	T577S	possibly damaging	Het
Clca4b	T	C	3: 144,630,940 (GRCm39)	R307G	possibly damaging	Het
Colgalt2	A	G	1: 152,360,620 (GRCm39)	Q219R	possibly damaging	Het
Cops3	A	T	11: 59,718,762 (GRCm39)	D177E	possibly damaging	Het
Cyp2d9	T	C	15: 82,338,256 (GRCm39)	Y127H	probably damaging	Het
Dnajc11	A	G	4: 152,052,983 (GRCm39)	T98A	probably damaging	Het
Dst	T	C	1: 34,265,058 (GRCm39)	S4757P	possibly damaging	Het
Dvl2	C	T	11: 69,898,957 (GRCm39)	T448I	possibly damaging	Het
E230016K23Rik	T	G	11: 83,512,496 (GRCm39)		noncoding transcript	Het
Efl1	G	T	7: 82,323,714 (GRCm39)	D219Y	probably damaging	Het
Epha10	T	A	4: 124,807,793 (GRCm39)		probably benign	Het
Fbxw22	T	A	9: 109,213,015 (GRCm39)	T311S	possibly damaging	Het
Folr1	T	A	7: 101,513,184 (GRCm39)	D37V	probably damaging	Het
Fshb	T	A	2: 106,889,224 (GRCm39)	I27F	probably damaging	Het
Gm8521	A	T	Y: 3,859,335 (GRCm39)		noncoding transcript	Het
Gpr45	T	C	1: 43,071,998 (GRCm39)	S214P	probably damaging	Het
Hgf	G	A	5: 16,771,860 (GRCm39)		probably null	Het
Hs6st1	T	C	1: 36,143,259 (GRCm39)	V398A	probably benign	Het
Ighmbp2	A	G	19: 3,321,646 (GRCm39)	V371A	probably damaging	Het
Kif18a	T	A	2: 109,148,370 (GRCm39)	N621K	probably benign	Het
Lama5	A	G	2: 179,822,911 (GRCm39)	F2785S	probably damaging	Het
Lcor	G	A	19: 41,574,791 (GRCm39)	G1182D	probably damaging	Het
Lrriq3	T	C	3: 154,835,108 (GRCm39)	I281T	possibly damaging	Het
Macf1	C	T	4: 123,367,229 (GRCm39)	A2511T	probably damaging	Het
Mark4	T	C	7: 19,170,886 (GRCm39)	D328G	possibly damaging	Het
Myo1c	G	A	11: 75,552,852 (GRCm39)	E434K	possibly damaging	Het
Nfe2l1	A	G	11: 96,710,205 (GRCm39)	S387P	probably damaging	Het
Nr1d2	A	G	14: 18,215,197 (GRCm38)	S272P	probably benign	Het
Nsrp1	T	C	11: 76,940,293 (GRCm39)	H104R	probably damaging	Het
Odf1	T	A	15: 38,219,863 (GRCm39)	S64T	probably benign	Het
Or2aa1	A	T	11: 59,480,477 (GRCm39)	V146E	probably damaging	Het
Or4c106	T	A	2: 88,683,122 (GRCm39)	I276K	probably damaging	Het
Pde4b	C	A	4: 102,278,985 (GRCm39)		probably benign	Het
Phox2a	C	T	7: 101,470,057 (GRCm39)	T96M	probably damaging	Het
Plg	A	G	17: 12,622,114 (GRCm39)	E478G	possibly damaging	Het
Polr3a	T	C	14: 24,505,009 (GRCm39)	I1084V	possibly damaging	Het
Ppp1r12c	T	A	7: 4,486,983 (GRCm39)	T517S	probably benign	Het
Ptk7	C	T	17: 46,883,603 (GRCm39)	V821M	probably damaging	Het
Ptpn6	C	T	6: 124,709,913 (GRCm39)	V2M	probably benign	Het
Rgl2	T	C	17: 34,152,529 (GRCm39)	V380A	probably benign	Het
Rpl13a-ps1	A	G	19: 50,018,591 (GRCm39)	V195A	possibly damaging	Het
Rsph1	C	A	17: 31,492,351 (GRCm39)	V72F	probably benign	Het
Sh3bp4	T	C	1: 89,073,072 (GRCm39)	V640A	probably benign	Het
Simc1	T	A	13: 54,672,795 (GRCm39)	V381E	probably benign	Het
Slc15a3	A	T	19: 10,833,296 (GRCm39)	T438S	probably damaging	Het
Slc5a4b	C	T	10: 75,898,233 (GRCm39)	V494M	probably benign	Het
Sos2	C	A	12: 69,674,058 (GRCm39)	R335L	probably benign	Het
Trp53i13	A	T	11: 77,399,566 (GRCm39)	N254K	probably damaging	Het
Trpc6	A	G	9: 8,609,922 (GRCm39)	Y130C	probably damaging	Het
Trpv1	A	G	11: 73,130,415 (GRCm39)	I174V	probably damaging	Het
Tsnax	T	A	8: 125,742,458 (GRCm39)	D62E	probably damaging	Het
Vezt	T	A	10: 93,806,193 (GRCm39)	E739D	probably benign	Het
Vmn1r59	T	A	7: 5,457,209 (GRCm39)	I184F	probably damaging	Het
Yeats2	T	C	16: 20,005,175 (GRCm39)	V385A	probably benign	Het

Other mutations in Or7g25

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL02104:Or7g25	APN	9	19,160,166 (GRCm39)	missense	probably benign	0.21
IGL02338:Or7g25	APN	9	19,159,960 (GRCm39)	missense	probably damaging	0.98
IGL03197:Or7g25	APN	9	19,160,098 (GRCm39)	missense	probably benign	0.37
IGL03227:Or7g25	APN	9	19,160,518 (GRCm39)	missense	probably damaging	1.00
IGL03046:Or7g25	UTSW	9	19,160,441 (GRCm39)	missense	probably damaging	0.99
IGL03052:Or7g25	UTSW	9	19,159,938 (GRCm39)	nonsense	probably null
R0423:Or7g25	UTSW	9	19,160,248 (GRCm39)	nonsense	probably null
R1316:Or7g25	UTSW	9	19,160,035 (GRCm39)	missense	probably benign	0.06
R1759:Or7g25	UTSW	9	19,160,384 (GRCm39)	missense	probably benign	0.36
R1782:Or7g25	UTSW	9	19,159,877 (GRCm39)	missense	probably benign	0.04
R2517:Or7g25	UTSW	9	19,160,357 (GRCm39)	missense	probably benign	0.21
R3196:Or7g25	UTSW	9	19,160,495 (GRCm39)	missense	probably damaging	1.00
R3742:Or7g25	UTSW	9	19,159,979 (GRCm39)	missense	possibly damaging	0.91
R4191:Or7g25	UTSW	9	19,160,383 (GRCm39)	missense	probably benign	0.05
R4320:Or7g25	UTSW	9	19,160,052 (GRCm39)	missense	probably damaging	1.00
R4523:Or7g25	UTSW	9	19,160,525 (GRCm39)	missense	probably damaging	0.98
R4756:Or7g25	UTSW	9	19,160,153 (GRCm39)	missense	possibly damaging	0.56
R4908:Or7g25	UTSW	9	19,160,149 (GRCm39)	missense	probably benign	0.00
R5554:Or7g25	UTSW	9	19,160,039 (GRCm39)	missense	probably benign	0.02
R6011:Or7g25	UTSW	9	19,159,807 (GRCm39)	missense	probably damaging	0.98
R7330:Or7g25	UTSW	9	19,160,567 (GRCm39)	missense	probably benign	0.01
R7862:Or7g25	UTSW	9	19,160,736 (GRCm39)	start gained	probably benign
R9544:Or7g25	UTSW	9	19,160,561 (GRCm39)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- CAGAGCAGGCATGTTTGATG -3'
(R):5'- TGGATTATCACCTCCATACACC -3'

Sequencing Primer
(F):5'- GGCATGTTTGATGACTTGAACAAG -3'
(R):5'- TTATCACCTCCATACACCCATGTAC -3'

Posted On

2016-07-22