Mutagenetix > Incidental Mutation

Incidental Mutation 'R0049:Or11g27'

40923

Institutional Source

Beutler Lab

Gene Symbol

Or11g27

Ensembl Gene

ENSMUSG00000094285

Gene Name

olfactory receptor family 11 subfamily G member 27

Synonyms

GA_x6K02T2PMLR-6243196-6244132, MOR106-8P, Olfr264, Olfr743, Olfr743-ps1, Olfr265, GA_x6K02T2N6FY-2320-2039, GA_x6K02T2N6FY-3870-3385, MOR106-14

MMRRC Submission

038343-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.086) question?

Stock #

R0049 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

50770871-50771806 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to T at 50771151 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Lysine to Methionine at position 94 (K94M)

Ref Sequence

ENSEMBL: ENSMUSP00000150946 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000071294] [ENSMUST00000215793]

AlphaFold

L7N1Y2

Predicted Effect

probably damaging
Transcript: ENSMUST00000071294
AA Change: K94M

PolyPhen 2 Score 0.999 (Sensitivity: 0.14; Specificity: 0.99)

SMART Domains

Protein: ENSMUSP00000071263
Gene: ENSMUSG00000094285
AA Change: K94M

Domain	Start	End	E-Value	Type
Pfam:7tm_4	35	311	1.6e-56	PFAM
Pfam:7tm_1	45	294	1e-21	PFAM

Predicted Effect

probably damaging
Transcript: ENSMUST00000215793
AA Change: K94M

PolyPhen 2 Score 0.999 (Sensitivity: 0.14; Specificity: 0.99)

Meta Mutation Damage Score

0.4051

Coding Region Coverage

1x: 99.7%
3x: 98.9%
10x: 97.0%
20x: 94.3%

Validation Efficiency

99% (114/115)

MGI Phenotype

FUNCTION: Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]

Allele List at MGI

Other mutations in this stock

Total: 110 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
A2m	A	T	6: 121,615,267 (GRCm39)	H9L	possibly damaging	Het
Aars1	T	A	8: 111,779,083 (GRCm39)	I739K	possibly damaging	Het
Abcb1b	T	A	5: 8,875,661 (GRCm39)	H611Q	probably damaging	Het
Acod1	T	A	14: 103,292,643 (GRCm39)	I389K	possibly damaging	Het
Adgre1	T	A	17: 57,709,841 (GRCm39)	L166*	probably null	Het
Akap1	C	A	11: 88,730,450 (GRCm39)		probably null	Het
Akna	T	A	4: 63,312,872 (GRCm39)	Q417L	probably damaging	Het
Anxa7	T	C	14: 20,512,678 (GRCm39)	D285G	probably damaging	Het
Arhgap1	T	C	2: 91,500,514 (GRCm39)	Y308H	probably damaging	Het
Arhgef10	A	C	8: 15,004,446 (GRCm39)	R360S	probably damaging	Het
Arhgef11	T	A	3: 87,636,500 (GRCm39)		probably null	Het
Arid3a	A	G	10: 79,766,899 (GRCm39)	T58A	possibly damaging	Het
Atosb	A	T	4: 43,036,441 (GRCm39)	S97T	probably benign	Het
Atp6v0a4	G	A	6: 38,059,016 (GRCm39)	R256C	probably damaging	Het
Camsap3	C	A	8: 3,648,772 (GRCm39)	S163R	probably benign	Het
Ccdc110	A	T	8: 46,395,663 (GRCm39)	E518V	probably damaging	Het
Ccdc180	G	A	4: 45,930,119 (GRCm39)		probably null	Het
Ccnt1	T	C	15: 98,462,960 (GRCm39)	M71V	probably benign	Het
Celsr2	T	A	3: 108,304,570 (GRCm39)	Y2263F	probably benign	Het
Cfap36	A	T	11: 29,196,514 (GRCm39)		probably null	Het
Cfap69	T	C	5: 5,663,734 (GRCm39)	T498A	probably benign	Het
Chadl	T	C	15: 81,578,213 (GRCm39)	D6G	probably benign	Het
Clstn3	T	A	6: 124,436,812 (GRCm39)	I132F	possibly damaging	Het
Cnot4	A	G	6: 35,028,212 (GRCm39)	V468A	probably benign	Het
Crmp1	T	G	5: 37,422,617 (GRCm39)	D141E	possibly damaging	Het
Crtc1	A	G	8: 70,844,509 (GRCm39)		probably null	Het
Cryz	C	A	3: 154,317,189 (GRCm39)	A136D	probably damaging	Het
Dph6	A	G	2: 114,353,525 (GRCm39)	V221A	probably benign	Het
Dst	A	T	1: 34,314,862 (GRCm39)	N4267Y	probably damaging	Het
Duox2	T	A	2: 122,127,167 (GRCm39)	D170V	possibly damaging	Het
Ecm2	A	T	13: 49,677,922 (GRCm39)	K403*	probably null	Het
Eif3d	T	C	15: 77,843,924 (GRCm39)	N474S	probably benign	Het
Elf1	T	C	14: 79,802,965 (GRCm39)	L106P	probably damaging	Het
Exoc4	G	C	6: 33,273,857 (GRCm39)		probably null	Het
F12	T	C	13: 55,574,130 (GRCm39)	D34G	probably benign	Het
Fam228b	A	T	12: 4,798,117 (GRCm39)	F200Y	probably damaging	Het
Fgl2	T	A	5: 21,580,661 (GRCm39)	D334E	possibly damaging	Het
Fras1	T	A	5: 96,924,481 (GRCm39)	F3641I	probably benign	Het
Gabrb2	T	G	11: 42,484,674 (GRCm39)	Y244D	probably damaging	Het
Gcc1	A	T	6: 28,421,268 (GRCm39)	D16E	probably benign	Het
Gga3	C	A	11: 115,477,915 (GRCm39)	G558*	probably null	Het
Glt1d1	T	C	5: 127,740,391 (GRCm39)		probably benign	Het
Gorasp2	T	C	2: 70,521,067 (GRCm39)	S346P	possibly damaging	Het
Hcn4	T	C	9: 58,767,582 (GRCm39)	S1048P	probably damaging	Het
Henmt1	T	A	3: 108,861,105 (GRCm39)		probably benign	Het
Htt	A	C	5: 35,066,006 (GRCm39)	K3060N	probably damaging	Het
Ibsp	C	T	5: 104,450,024 (GRCm39)	L8F	probably damaging	Het
Kif27	A	T	13: 58,451,378 (GRCm39)	D983E	probably damaging	Het
Kif3a	T	A	11: 53,481,560 (GRCm39)		probably benign	Het
Kif3c	A	C	12: 3,417,090 (GRCm39)	K370N	possibly damaging	Het
Loxhd1	T	C	18: 77,468,256 (GRCm39)		probably benign	Het
Maz	A	T	7: 126,623,758 (GRCm39)	D74E	probably damaging	Het
Med21	T	C	6: 146,551,732 (GRCm39)	S128P	probably damaging	Het
Mms19	A	C	19: 41,943,607 (GRCm39)	M374R	probably damaging	Het
Mprip	T	C	11: 59,657,571 (GRCm39)	V801A	probably damaging	Het
Mrpl3	T	C	9: 104,932,872 (GRCm39)	V111A	probably benign	Het
Mtfr2	T	A	10: 20,224,158 (GRCm39)	Y31N	probably damaging	Het
Myh3	C	T	11: 66,990,498 (GRCm39)	R1677C	probably damaging	Het
Mynn	T	A	3: 30,661,230 (GRCm39)	*61K	probably null	Het
Neb	A	C	2: 52,060,479 (GRCm39)	M2286R	possibly damaging	Het
Ngf	A	T	3: 102,427,661 (GRCm39)	R137*	probably null	Het
Nr1i3	T	A	1: 171,041,982 (GRCm39)	V22E	probably damaging	Het
Nxpe5	T	C	5: 138,249,566 (GRCm39)	V452A	probably damaging	Het
Oas1e	C	A	5: 120,933,395 (GRCm39)	A57S	probably benign	Het
Or14j10	T	A	17: 37,935,024 (GRCm39)	R167S	probably benign	Het
Or5b105	G	A	19: 13,080,642 (GRCm39)	R3C	possibly damaging	Het
Pax3	A	G	1: 78,080,141 (GRCm39)	L415P	probably damaging	Het
Pcnt	G	T	10: 76,205,655 (GRCm39)		probably benign	Het
Peg3	G	T	7: 6,714,672 (GRCm39)	D183E	possibly damaging	Het
Pglyrp1	G	T	7: 18,623,313 (GRCm39)	G120V	probably damaging	Het
Pnp2	T	A	14: 51,196,990 (GRCm39)	Y25*	probably null	Het
Pomt1	T	A	2: 32,142,023 (GRCm39)	H584Q	possibly damaging	Het
Ppp1r12a	A	G	10: 108,089,193 (GRCm39)	N611D	possibly damaging	Het
Prkcq	G	A	2: 11,288,643 (GRCm39)	G532E	probably benign	Het
Prl6a1	A	T	13: 27,501,980 (GRCm39)	I116F	probably damaging	Het
Ptprh	T	A	7: 4,576,361 (GRCm39)	T300S	possibly damaging	Het
Pwp1	A	G	10: 85,721,480 (GRCm39)	T361A	possibly damaging	Het
Rab4a	A	T	8: 124,554,081 (GRCm39)	H5L	probably damaging	Het
Raet1e	C	A	10: 22,056,761 (GRCm39)	H112Q	possibly damaging	Het
Ramp1	T	C	1: 91,124,592 (GRCm39)	I51T	possibly damaging	Het
Raph1	G	T	1: 60,565,058 (GRCm39)	T143K	probably benign	Het
Rhpn1	A	G	15: 75,581,088 (GRCm39)	E110G	possibly damaging	Het
Rnf168	A	T	16: 32,117,287 (GRCm39)	T283S	possibly damaging	Het
Ros1	T	A	10: 51,977,857 (GRCm39)	Y1463F	possibly damaging	Het
Rtn4ip1	A	G	10: 43,797,430 (GRCm39)	Q223R	probably null	Het
Rtp4	G	T	16: 23,431,679 (GRCm39)	M70I	probably benign	Het
Sag	C	A	1: 87,762,340 (GRCm39)	T335K	probably damaging	Het
Satb1	T	A	17: 52,047,374 (GRCm39)	Q647L	probably benign	Het
Sec31b	T	C	19: 44,508,847 (GRCm39)		probably benign	Het
Sgo1	C	T	17: 53,986,691 (GRCm39)	D167N	probably damaging	Het
Slco1a8	T	C	6: 141,936,147 (GRCm39)	T313A	probably benign	Het
Smchd1	T	C	17: 71,738,231 (GRCm39)	I545V	probably benign	Het
St6gal1	G	T	16: 23,139,891 (GRCm39)	A21S	probably damaging	Het
Stard9	C	A	2: 120,530,300 (GRCm39)	L2186I	probably damaging	Het
Sun2	T	A	15: 79,611,810 (GRCm39)		probably benign	Het
Taf4	G	A	2: 179,565,884 (GRCm39)	T849M	probably damaging	Het
Tdrd5	A	T	1: 156,129,473 (GRCm39)	I79N	probably damaging	Het
Tdrd7	A	G	4: 45,987,582 (GRCm39)	I72V	probably damaging	Het
Tnxb	T	A	17: 34,928,542 (GRCm39)	V2652E	possibly damaging	Het
Trim30a	C	T	7: 104,078,559 (GRCm39)		probably null	Het
Tshz3	A	G	7: 36,469,534 (GRCm39)	T508A	probably damaging	Het
Ttc21b	A	G	2: 66,053,908 (GRCm39)	L757P	probably damaging	Het
Ubtd2	A	C	11: 32,449,223 (GRCm39)		probably null	Het
Ubtd2	G	T	11: 32,449,224 (GRCm39)		probably null	Het
Vmn1r218	C	T	13: 23,321,225 (GRCm39)	Q111*	probably null	Het
Vmn2r75	G	A	7: 85,797,309 (GRCm39)	Q835*	probably null	Het
Vwa8	T	A	14: 79,331,179 (GRCm39)	M1229K	probably benign	Het
Wdr76	C	T	2: 121,349,932 (GRCm39)	R111C	probably damaging	Het
Xcr1	T	A	9: 123,684,940 (GRCm39)	D274V	possibly damaging	Het
Ypel5	C	T	17: 73,153,332 (GRCm39)	T12I	probably benign	Het

Other mutations in Or11g27

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01128:Or11g27	APN	14	50,771,406 (GRCm39)	missense	probably damaging	1.00
IGL01551:Or11g27	APN	14	50,771,618 (GRCm39)	missense	probably benign
IGL02024:Or11g27	APN	14	50,771,307 (GRCm39)	missense	probably benign	0.00
IGL02867:Or11g27	APN	14	50,770,970 (GRCm39)	missense	probably benign
IGL02889:Or11g27	APN	14	50,770,970 (GRCm39)	missense	probably benign
IGL03195:Or11g27	APN	14	50,770,877 (GRCm39)	missense	probably benign
IGL03296:Or11g27	APN	14	50,771,402 (GRCm39)	missense	possibly damaging	0.90
R0049:Or11g27	UTSW	14	50,771,151 (GRCm39)	missense	probably damaging	1.00
R0102:Or11g27	UTSW	14	50,771,088 (GRCm39)	missense	probably damaging	1.00
R0556:Or11g27	UTSW	14	50,771,381 (GRCm39)	missense	probably benign	0.01
R0626:Or11g27	UTSW	14	50,771,159 (GRCm39)	missense	possibly damaging	0.78
R0661:Or11g27	UTSW	14	50,771,552 (GRCm39)	missense	probably benign
R0759:Or11g27	UTSW	14	50,771,159 (GRCm39)	missense	possibly damaging	0.78
R0761:Or11g27	UTSW	14	50,771,159 (GRCm39)	missense	possibly damaging	0.78
R0894:Or11g27	UTSW	14	50,771,159 (GRCm39)	missense	possibly damaging	0.78
R1109:Or11g27	UTSW	14	50,771,159 (GRCm39)	missense	possibly damaging	0.78
R1110:Or11g27	UTSW	14	50,771,159 (GRCm39)	missense	possibly damaging	0.78
R1312:Or11g27	UTSW	14	50,771,652 (GRCm39)	missense	probably benign
R1446:Or11g27	UTSW	14	50,771,159 (GRCm39)	missense	possibly damaging	0.78
R1470:Or11g27	UTSW	14	50,771,159 (GRCm39)	missense	possibly damaging	0.78
R1470:Or11g27	UTSW	14	50,771,159 (GRCm39)	missense	possibly damaging	0.78
R1502:Or11g27	UTSW	14	50,771,234 (GRCm39)	missense	possibly damaging	0.47
R1518:Or11g27	UTSW	14	50,771,622 (GRCm39)	missense	probably damaging	1.00
R1529:Or11g27	UTSW	14	50,771,159 (GRCm39)	missense	possibly damaging	0.78
R1624:Or11g27	UTSW	14	50,771,100 (GRCm39)	missense	probably damaging	1.00
R1646:Or11g27	UTSW	14	50,771,040 (GRCm39)	missense	probably benign	0.01
R1687:Or11g27	UTSW	14	50,771,159 (GRCm39)	missense	possibly damaging	0.78
R1795:Or11g27	UTSW	14	50,771,159 (GRCm39)	missense	possibly damaging	0.78
R2011:Or11g27	UTSW	14	50,771,141 (GRCm39)	missense	probably damaging	1.00
R2120:Or11g27	UTSW	14	50,771,403 (GRCm39)	missense	probably damaging	1.00
R2697:Or11g27	UTSW	14	50,771,238 (GRCm39)	missense	probably damaging	1.00
R2857:Or11g27	UTSW	14	50,770,897 (GRCm39)	missense	probably benign	0.19
R2858:Or11g27	UTSW	14	50,770,897 (GRCm39)	missense	probably benign	0.19
R3906:Or11g27	UTSW	14	50,771,211 (GRCm39)	missense	probably benign	0.03
R4327:Or11g27	UTSW	14	50,770,971 (GRCm39)	missense	probably benign	0.05
R4355:Or11g27	UTSW	14	50,771,216 (GRCm39)	missense	possibly damaging	0.94
R4663:Or11g27	UTSW	14	50,771,061 (GRCm39)	missense	probably damaging	1.00
R5214:Or11g27	UTSW	14	50,771,804 (GRCm39)	makesense	probably null
R5964:Or11g27	UTSW	14	50,771,655 (GRCm39)	missense	probably damaging	0.99
R6148:Or11g27	UTSW	14	50,771,778 (GRCm39)	missense	probably benign	0.00
R6167:Or11g27	UTSW	14	50,771,612 (GRCm39)	missense	probably damaging	1.00
R6301:Or11g27	UTSW	14	50,771,711 (GRCm39)	missense	probably benign	0.02
R6616:Or11g27	UTSW	14	50,771,364 (GRCm39)	missense	probably benign	0.43
R6910:Or11g27	UTSW	14	50,771,330 (GRCm39)	missense	probably benign	0.31
R7076:Or11g27	UTSW	14	50,771,278 (GRCm39)	nonsense	probably null
R7483:Or11g27	UTSW	14	50,771,472 (GRCm39)	missense	probably benign	0.06
R7574:Or11g27	UTSW	14	50,771,770 (GRCm39)	missense	probably benign	0.01
R7731:Or11g27	UTSW	14	50,771,141 (GRCm39)	missense	probably damaging	0.99
R8691:Or11g27	UTSW	14	50,770,910 (GRCm39)	missense	probably benign	0.01
R9072:Or11g27	UTSW	14	50,771,211 (GRCm39)	missense	probably benign	0.03
R9073:Or11g27	UTSW	14	50,771,211 (GRCm39)	missense	probably benign	0.03
R9321:Or11g27	UTSW	14	50,771,471 (GRCm39)	missense	probably benign	0.01
R9478:Or11g27	UTSW	14	50,771,051 (GRCm39)	missense	probably benign	0.01
R9557:Or11g27	UTSW	14	50,771,552 (GRCm39)	missense	probably benign

Predicted Primers

PCR Primer
(F):5'- TGGTGCTTTCACAGAACACAGACAG -3'
(R):5'- ACTGATCACAAGGATGTTGCAGAGG -3'

Sequencing Primer
(F):5'- ACAGGCTTCATCCTTTTGGG -3'
(R):5'- GCGCCTAGTCATGAGAGAAG -3'

Posted On

2013-05-23