Incidental Mutation 'IGL03174:Map3k8'
Institutional Source Australian Phenomics Network (link to record)
Gene Symbol Map3k8
Ensembl Gene ENSMUSG00000024235
Gene Namemitogen-activated protein kinase kinase kinase 8
SynonymsTpl2, Tpl-2, c-COT, Cot, Cot/Tpl2
Accession Numbers
Is this an essential gene? Non essential (E-score: 0.000) question?
Stock #IGL03174
Quality Score
Chromosomal Location4331327-4353015 bp(-) (GRCm38)
Type of Mutationmissense
DNA Base Change (assembly) A to G at 4349247 bp
Amino Acid Change Serine to Proline at position 24 (S24P)
Ref Sequence ENSEMBL: ENSMUSP00000133469 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000025078] [ENSMUST00000173930]
Predicted Effect probably damaging
Transcript: ENSMUST00000025078
AA Change: S24P

PolyPhen 2 Score 0.965 (Sensitivity: 0.78; Specificity: 0.95)
SMART Domains Protein: ENSMUSP00000025078
Gene: ENSMUSG00000024235
AA Change: S24P

Pfam:Pkinase 137 388 1.1e-47 PFAM
Pfam:Pkinase_Tyr 139 386 4.6e-26 PFAM
Predicted Effect noncoding transcript
Transcript: ENSMUST00000172805
Predicted Effect probably damaging
Transcript: ENSMUST00000173930
AA Change: S24P

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)
SMART Domains Protein: ENSMUSP00000133469
Gene: ENSMUSG00000024235
AA Change: S24P

SCOP:d1phk__ 146 169 2e-4 SMART
Coding Region Coverage
Validation Efficiency
MGI Phenotype FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene is an oncogene that encodes a member of the serine/threonine protein kinase family. The encoded protein localizes to the cytoplasm and can activate both the MAP kinase and JNK kinase pathways. This protein was shown to activate IkappaB kinases, and thus induce the nuclear production of NF-kappaB. This protein was also found to promote the production of TNF-alpha and IL-2 during T lymphocyte activation. This gene may also utilize a downstream in-frame translation start codon, and thus produce an isoform containing a shorter N-terminus. The shorter isoform has been shown to display weaker transforming activity. Alternate splicing results in multiple transcript variants that encode the same protein. [provided by RefSeq, Sep 2011]
PHENOTYPE: Mutant mice resist endotoxic shock. Their MHC II expression is enhanced. Macrophages' TNF-alpha response to viruses and to all TLR ligands is impaired. Macrophage and T-cell secretion of other cytokines in response to various TLR ligands or OVA is aberrant. Anti-OVA Ig classes are abnormally skewed. [provided by MGI curators]
Allele List at MGI
Other mutations in this stock
Total: 30 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
1700022I11Rik T C 4: 42,970,975 Y67H probably benign Het
Abcb1b T C 5: 8,827,752 L703P probably benign Het
Abi3bp A G 16: 56,614,747 K602R possibly damaging Het
Ank2 T C 3: 126,940,095 D622G probably damaging Het
Bpifb1 T A 2: 154,213,049 L287Q probably damaging Het
Brd8 T C 18: 34,604,909 E774G probably damaging Het
C87977 C T 4: 144,208,430 S247N probably benign Het
Cnot1 A G 8: 95,761,355 S558P probably damaging Het
Col15a1 T C 4: 47,282,666 I781T probably damaging Het
Cyp2j7 T A 4: 96,195,370 R448* probably null Het
Dgkb A T 12: 38,216,054 I511F possibly damaging Het
Fam221b T C 4: 43,666,542 E23G probably benign Het
Gm17067 C A 7: 42,710,676 probably null Het
Grin3a T A 4: 49,771,107 D555V probably damaging Het
Hid1 T C 11: 115,360,389 Y107C probably damaging Het
Kcnd2 A G 6: 21,216,516 D73G possibly damaging Het
Kit A G 5: 75,607,113 D52G probably benign Het
Llgl1 G T 11: 60,706,210 R189L probably benign Het
Lrp2 T A 2: 69,466,265 D3315V probably damaging Het
Nckap5 A G 1: 125,981,646 L1616P probably damaging Het
Nrcam A G 12: 44,576,006 probably benign Het
Olfr1475 A G 19: 13,480,069 M43T probably benign Het
Olfr715b A T 7: 107,106,270 M197K probably benign Het
Polr1a G A 6: 71,977,347 R1595H possibly damaging Het
Rusc1 T C 3: 89,091,770 D235G probably damaging Het
Serpinb6e A T 13: 33,836,480 I215N probably damaging Het
Sfrp4 A T 13: 19,632,207 Q137L probably benign Het
Vmn2r2 C T 3: 64,117,123 W679* probably null Het
Vps8 T A 16: 21,466,463 L398H probably damaging Het
Zfp709 A T 8: 71,889,026 T99S probably benign Het
Other mutations in Map3k8
AlleleSourceChrCoordTypePredicted EffectPPH Score
IGL02458:Map3k8 APN 18 4334660 missense probably damaging 1.00
IGL02483:Map3k8 APN 18 4349318 utr 5 prime probably benign
Flojo UTSW 18 4339548 missense possibly damaging 0.95
gnostic_gospel UTSW 18 4333965 missense probably damaging 1.00
juicy UTSW 18 4339552 missense probably damaging 0.99
Sluggish UTSW 18 4339608 splice site probably benign
R0304:Map3k8 UTSW 18 4339552 missense probably damaging 0.99
R0569:Map3k8 UTSW 18 4349162 missense probably benign 0.00
R1748:Map3k8 UTSW 18 4334766 missense probably damaging 1.00
R1793:Map3k8 UTSW 18 4332389 nonsense probably null
R2310:Map3k8 UTSW 18 4349001 missense probably benign
R3625:Map3k8 UTSW 18 4333965 missense probably damaging 1.00
R4786:Map3k8 UTSW 18 4340647 nonsense probably null
R4921:Map3k8 UTSW 18 4349124 missense possibly damaging 0.92
R4930:Map3k8 UTSW 18 4349215 nonsense probably null
R4934:Map3k8 UTSW 18 4339548 missense possibly damaging 0.95
R4956:Map3k8 UTSW 18 4339530 missense probably benign 0.00
R5241:Map3k8 UTSW 18 4340750 missense probably damaging 0.98
R5549:Map3k8 UTSW 18 4340762 missense probably damaging 0.98
R6317:Map3k8 UTSW 18 4348979 critical splice donor site probably null
R6326:Map3k8 UTSW 18 4340651 missense probably damaging 1.00
R6910:Map3k8 UTSW 18 4340801 missense probably benign 0.03
R7010:Map3k8 UTSW 18 4334060 missense probably damaging 1.00
R7247:Map3k8 UTSW 18 4334036 missense probably damaging 1.00
R7300:Map3k8 UTSW 18 4349076 missense probably damaging 0.98
R7348:Map3k8 UTSW 18 4340561 missense probably damaging 1.00
Posted On2016-08-02