Incidental Mutation 'R0458:Aurka'
ID 41285
Institutional Source Beutler Lab
Gene Symbol Aurka
Ensembl Gene ENSMUSG00000027496
Gene Name aurora kinase A
Synonyms Stk6, IAK1, IAK, AIRK1, Aurora-A, Ark1, Ayk1, aurora A
MMRRC Submission 038658-MU
Accession Numbers
Essential gene? Essential (E-score: 1.000) question?
Stock # R0458 (G1)
Quality Score 225
Status Validated
Chromosome 2
Chromosomal Location 172198110-172212455 bp(-) (GRCm39)
Type of Mutation nonsense
DNA Base Change (assembly) C to A at 172212366 bp (GRCm39)
Zygosity Heterozygous
Amino Acid Change Glutamic Acid to Stop codon at position 4 (E4*)
Ref Sequence ENSEMBL: ENSMUSP00000028997 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000028997] [ENSMUST00000109139] [ENSMUST00000109140] [ENSMUST00000116375] [ENSMUST00000151511]
AlphaFold P97477
PDB Structure Crystal structure of mouse Aurora A (Asn186->Gly, Lys240->Arg, Met302->Leu) in complex with 1-{5-[2-(thieno[3,2-d]pyrimidin-4-ylamino)-ethyl]- thiazol-2-yl}-3-(3-trifluoromethyl-phenyl)-urea [X-RAY DIFFRACTION]
Crystal structure of mouse Aurora A (Asn186->Gly, Lys240->Arg, Met302->Leu) in complex with 1-(3-chloro-phenyl)-3-{5-[2-(thieno[3,2-d]pyrimidin-4-ylamino)- ethyl]-thiazol-2-yl}-urea [SNS-314] [X-RAY DIFFRACTION]
Crystal structure of mouse Aurora A (Asn186->Gly, Lys240->Arg, Met302->Leu) in complex with 1-{5-[2-(1-methyl-1H-pyrazolo[4,3-d]pyrimidin-7-ylamino)-ethyl]-thiazol-2-yl}-3-(3-trifluoromethyl-phenyl)-urea [X-RAY DIFFRACTION]
Crystal structure of mouse Aurora A (Asn186->Gly, Lys240->Arg, Met302->Leu) in complex with [7-(2-{2-[3-(3-chloro-phenyl)-ureido]-thiazol-5-yl}-ethylamino)-pyrazolo[4,3-d]pyrimidin-1-yl]-acetic acid [X-RAY DIFFRACTION]
Crystal structure of Aurora A complexed with an inhibitor discovered through site-directed dynamic tethering [X-RAY DIFFRACTION]
Crystal structure of the mouse Aurora-A catalytic domain (Asn186->Gly, Lys240->Arg, Met302->Leu) in complex with Compound 290. [X-RAY DIFFRACTION]
Crystal structure of the mouse Aurora-A catalytic domain (Asn186->Gly, Lys240->Arg, Met302->Leu) in complex with Compound 823. [X-RAY DIFFRACTION]
Crystal structure of the mouse Aurora-A catalytic domain (Asn186->Gly, Lys240->Arg, Met302->Leu) in complex with Compound 130. [X-RAY DIFFRACTION]
Predicted Effect probably null
Transcript: ENSMUST00000028997
AA Change: E4*
SMART Domains Protein: ENSMUSP00000028997
Gene: ENSMUSG00000027496
AA Change: E4*

DomainStartEndE-ValueType
S_TKc 146 396 2.25e-99 SMART
Predicted Effect probably benign
Transcript: ENSMUST00000109139
SMART Domains Protein: ENSMUSP00000104767
Gene: ENSMUSG00000027496

DomainStartEndE-ValueType
S_TKc 124 374 2.25e-99 SMART
Predicted Effect probably benign
Transcript: ENSMUST00000109140
SMART Domains Protein: ENSMUSP00000104768
Gene: ENSMUSG00000027496

DomainStartEndE-ValueType
S_TKc 124 374 2.25e-99 SMART
Predicted Effect probably benign
Transcript: ENSMUST00000116375
SMART Domains Protein: ENSMUSP00000112076
Gene: ENSMUSG00000027498

DomainStartEndE-ValueType
Pfam:CSTF1_dimer 6 62 5.4e-28 PFAM
WD40 97 136 2.61e-3 SMART
WD40 162 201 3.29e-9 SMART
WD40 206 245 6.88e0 SMART
WD40 248 290 9.02e-7 SMART
WD40 293 334 1.44e-5 SMART
Blast:WD40 337 382 8e-9 BLAST
WD40 385 425 2.49e-1 SMART
Predicted Effect noncoding transcript
Transcript: ENSMUST00000126107
Predicted Effect probably benign
Transcript: ENSMUST00000151511
SMART Domains Protein: ENSMUSP00000121178
Gene: ENSMUSG00000027498

DomainStartEndE-ValueType
PDB:2XZ2|A 8 59 2e-7 PDB
WD40 97 136 2.61e-3 SMART
Meta Mutation Damage Score 0.9755 question?
Coding Region Coverage
  • 1x: 99.7%
  • 3x: 98.9%
  • 10x: 97.0%
  • 20x: 94.3%
Validation Efficiency 100% (79/79)
MGI Phenotype FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] The protein encoded by this gene is a cell cycle-regulated kinase that appears to be involved in microtubule formation and/or stabilization at the spindle pole during chromosome segregation. The encoded protein is found at the centrosome in interphase cells and at the spindle poles in mitosis. This gene may play a role in tumor development and progression. A processed pseudogene of this gene has been found on chromosome 1, and an unprocessed pseudogene has been found on chromosome 10. Multiple transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]
PHENOTYPE: Mice homozygous for a null allele display embryonic lethality before implantation, early embryonic growth arrest, and impaired mitosis. Heterozygous null mice display increased incidence of tumors primarily lymphomas and chromosomal instability. [provided by MGI curators]
Allele List at MGI

All alleles(25) : Targeted(4) Gene trapped(21)
Other mutations in this stock
Total: 76 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
4933407L21Rik A G 1: 85,856,747 (GRCm39) E8G unknown Het
9130008F23Rik T C 17: 41,191,127 (GRCm39) T101A probably benign Het
Abcb8 C T 5: 24,611,231 (GRCm39) T455I probably benign Het
Abcb9 C A 5: 124,220,209 (GRCm39) probably null Het
Akp3 T G 1: 87,054,259 (GRCm39) Y265* probably null Het
Atp6v1b1 A T 6: 83,729,390 (GRCm39) D109V probably damaging Het
Cacna1g T A 11: 94,300,266 (GRCm39) Q2168L probably damaging Het
Cdc45 T A 16: 18,600,722 (GRCm39) probably benign Het
Cfap61 T C 2: 145,850,837 (GRCm39) V325A probably benign Het
Clasp2 T A 9: 113,735,292 (GRCm39) probably null Het
Crim1 T A 17: 78,620,655 (GRCm39) I365N probably damaging Het
Dcaf8 A G 1: 172,001,610 (GRCm39) N269S probably benign Het
Dnaaf5 T C 5: 139,147,633 (GRCm39) V399A possibly damaging Het
Ear2 A G 14: 44,340,705 (GRCm39) Y121C probably damaging Het
Eef2k T C 7: 120,502,513 (GRCm39) Y692H probably damaging Het
Elavl2 A T 4: 91,197,104 (GRCm39) probably benign Het
Epn2 C A 11: 61,437,281 (GRCm39) R97L possibly damaging Het
Fam243 T C 16: 92,117,995 (GRCm39) I98V probably benign Het
Fzd6 G A 15: 38,894,676 (GRCm39) A281T probably damaging Het
Garem2 T A 5: 30,319,180 (GRCm39) I214N probably damaging Het
Glg1 A G 8: 111,887,238 (GRCm39) probably benign Het
Golm1 T C 13: 59,812,178 (GRCm39) E48G probably damaging Het
Gpaa1 G T 15: 76,216,233 (GRCm39) R12L probably benign Het
Gstm1 T A 3: 107,924,679 (GRCm39) T34S probably benign Het
Gtf3c1 G A 7: 125,243,306 (GRCm39) P1766L possibly damaging Het
Herc1 A T 9: 66,383,663 (GRCm39) Q3709L probably benign Het
Hoxa13 CCG CCGCG 6: 52,237,618 (GRCm39) probably null Het
Icam1 A G 9: 20,939,157 (GRCm39) probably null Het
Itga9 T C 9: 118,510,096 (GRCm39) probably null Het
Kif15 T C 9: 122,838,424 (GRCm39) F1121L probably benign Het
Klhl30 T A 1: 91,288,718 (GRCm39) probably benign Het
Ldlrad1 T C 4: 107,073,387 (GRCm39) C141R probably damaging Het
Lemd2 T C 17: 27,409,627 (GRCm39) D508G probably damaging Het
Lilra5 A C 7: 4,241,218 (GRCm39) T52P probably benign Het
Lrtm2 G A 6: 119,294,229 (GRCm39) P301S probably damaging Het
Mcoln2 A G 3: 145,855,768 (GRCm39) probably benign Het
Mkrn2os A G 6: 115,563,631 (GRCm39) S135P probably damaging Het
Mlxipl T C 5: 135,162,224 (GRCm39) V607A probably benign Het
Mmadhc T C 2: 50,171,173 (GRCm39) Y213C probably benign Het
Mpo C A 11: 87,687,123 (GRCm39) A223E probably benign Het
Mthfd2l C G 5: 91,168,036 (GRCm39) I310M probably damaging Het
Muc5b C A 7: 141,418,709 (GRCm39) A3885D probably benign Het
Mvp A G 7: 126,597,663 (GRCm39) W152R probably damaging Het
Nmur2 A T 11: 55,931,394 (GRCm39) F106I possibly damaging Het
Nr3c2 T A 8: 77,636,167 (GRCm39) F423I probably damaging Het
Or1l8 A G 2: 36,817,349 (GRCm39) V259A probably damaging Het
Or5m5 A G 2: 85,814,600 (GRCm39) S139G probably benign Het
Or8c16 G A 9: 38,130,344 (GRCm39) C75Y probably damaging Het
Or9q1 G T 19: 13,805,593 (GRCm39) H56N probably benign Het
Pappa A G 4: 65,074,119 (GRCm39) I224M probably damaging Het
Prex1 A C 2: 166,427,743 (GRCm39) S800A probably damaging Het
Prkaca T C 8: 84,721,911 (GRCm39) probably benign Het
Ptpru A T 4: 131,526,986 (GRCm39) V662E possibly damaging Het
Rabep1 T A 11: 70,777,824 (GRCm39) probably null Het
Rbms2 C T 10: 127,987,058 (GRCm39) C50Y probably damaging Het
Rd3 C T 1: 191,709,414 (GRCm39) P25S probably damaging Het
Rnf148 T G 6: 23,654,256 (GRCm39) I247L probably benign Het
Sf3b3 A G 8: 111,538,768 (GRCm39) probably benign Het
Slc35c1 A T 2: 92,284,858 (GRCm39) F252Y probably damaging Het
Slc38a11 T C 2: 65,193,813 (GRCm39) probably null Het
Snx6 G T 12: 54,814,921 (GRCm39) Y17* probably null Het
Sox6 C A 7: 115,089,029 (GRCm39) R611L probably damaging Het
Spata13 G A 14: 60,929,492 (GRCm39) R350H probably damaging Het
Sppl2a G T 2: 126,746,879 (GRCm39) A483D probably damaging Het
Stat1 C T 1: 52,188,211 (GRCm39) probably benign Het
Tab2 A T 10: 7,795,319 (GRCm39) Y314N probably damaging Het
Tor1aip1 T C 1: 155,906,153 (GRCm39) N213S probably damaging Het
Trim39 T C 17: 36,572,404 (GRCm39) K300E probably damaging Het
Tubal3 T C 13: 3,983,137 (GRCm39) S306P probably damaging Het
Ufm1 A G 3: 53,768,655 (GRCm39) L33P probably damaging Het
Washc4 G A 10: 83,382,663 (GRCm39) V26I possibly damaging Het
Wfs1 A G 5: 37,126,013 (GRCm39) Y293H probably damaging Het
Zbtb41 T C 1: 139,351,214 (GRCm39) V109A probably damaging Het
Zfp667 T C 7: 6,307,844 (GRCm39) S171P probably benign Het
Zkscan5 T A 5: 145,142,281 (GRCm39) H59Q probably damaging Het
Zswim8 C T 14: 20,768,965 (GRCm39) R1128W probably damaging Het
Other mutations in Aurka
AlleleSourceChrCoordTypePredicted EffectPPH Score
IGL01458:Aurka APN 2 172,210,899 (GRCm39) unclassified probably benign
IGL02338:Aurka APN 2 172,201,778 (GRCm39) missense probably benign 0.00
IGL02894:Aurka APN 2 172,208,868 (GRCm39) splice site probably null
IGL03188:Aurka APN 2 172,205,688 (GRCm39) missense possibly damaging 0.60
G1Funyon:Aurka UTSW 2 172,198,850 (GRCm39) missense probably damaging 0.99
PIT4585001:Aurka UTSW 2 172,199,117 (GRCm39) missense probably benign 0.01
R0006:Aurka UTSW 2 172,201,673 (GRCm39) critical splice donor site probably null
R0006:Aurka UTSW 2 172,201,673 (GRCm39) critical splice donor site probably null
R0555:Aurka UTSW 2 172,209,067 (GRCm39) missense probably benign 0.07
R1130:Aurka UTSW 2 172,199,178 (GRCm39) splice site probably null
R1140:Aurka UTSW 2 172,199,149 (GRCm39) missense probably damaging 1.00
R2507:Aurka UTSW 2 172,212,365 (GRCm39) missense probably benign 0.00
R2887:Aurka UTSW 2 172,209,040 (GRCm39) missense probably benign 0.01
R2889:Aurka UTSW 2 172,209,040 (GRCm39) missense probably benign 0.01
R3772:Aurka UTSW 2 172,208,880 (GRCm39) missense probably benign
R4929:Aurka UTSW 2 172,212,326 (GRCm39) missense probably benign 0.05
R5409:Aurka UTSW 2 172,209,036 (GRCm39) missense possibly damaging 0.78
R6158:Aurka UTSW 2 172,205,516 (GRCm39) critical splice donor site probably null
R6689:Aurka UTSW 2 172,212,313 (GRCm39) critical splice donor site probably null
R6828:Aurka UTSW 2 172,199,172 (GRCm39) missense probably damaging 1.00
R7912:Aurka UTSW 2 172,210,949 (GRCm39) missense probably benign 0.00
R8066:Aurka UTSW 2 172,212,338 (GRCm39) missense probably benign 0.00
R8301:Aurka UTSW 2 172,198,850 (GRCm39) missense probably damaging 0.99
R9764:Aurka UTSW 2 172,201,760 (GRCm39) missense probably damaging 1.00
Predicted Primers PCR Primer
(F):5'- AATAAGCCGCAGTTCCCGCATC -3'
(R):5'- CGAGCTTCCCATAGTCGCGTTTAC -3'

Sequencing Primer
(F):5'- CGCATCCACCATCGGTTTG -3'
(R):5'- TTTACGGTGAGCCAATCACAG -3'
Posted On 2013-05-23