Incidental Mutation 'IGL03207:Capn2'
| ID |
413171 |
| Institutional Source |
Australian Phenomics Network
(link to record)
|
| Gene Symbol |
Capn2
|
| Ensembl Gene |
ENSMUSG00000026509 |
| Gene Name |
calpain 2 |
| Synonyms |
Capa2, Capa-2, m-calpain |
| Accession Numbers |
|
| Essential gene? |
Essential
(E-score: 1.000)
|
| Stock # |
IGL03207
|
| Quality Score |
|
|
Status
|
|
| Chromosome |
1 |
| Chromosomal Location |
182294825-182345173 bp(-) (GRCm39) |
| Type of Mutation |
missense |
| DNA Base Change (assembly) |
T to G
at 182316578 bp (GRCm39)
|
| Zygosity |
Heterozygous |
| Amino Acid Change |
Threonine to Proline
at position 294
(T294P)
|
| Ref Sequence |
ENSEMBL: ENSMUSP00000068895
(fasta)
|
| Gene Model |
predicted gene model for transcript(s):
[ENSMUST00000068505]
|
| AlphaFold |
O08529 |
| Predicted Effect |
possibly damaging
Transcript: ENSMUST00000068505
AA Change: T294P
PolyPhen 2
Score 0.918 (Sensitivity: 0.81; Specificity: 0.94)
|
| SMART Domains |
Protein: ENSMUSP00000068895
Gene: ENSMUSG00000026509
AA Change: T294P
| Domain | Start | End | E-Value | Type |
|---|
|
CysPc
|
27 |
352 |
1.62e-186 |
SMART |
|
calpain_III
|
355 |
510 |
3.47e-90 |
SMART |
|
low complexity region
|
513 |
532 |
N/A |
INTRINSIC |
|
EFh
|
576 |
604 |
5.86e0 |
SMART |
|
EFh
|
606 |
634 |
3.21e0 |
SMART |
|
| Predicted Effect |
noncoding transcript
Transcript: ENSMUST00000192230
|
| Predicted Effect |
noncoding transcript
Transcript: ENSMUST00000192483
|
| Predicted Effect |
noncoding transcript
Transcript: ENSMUST00000192568
|
| Predicted Effect |
noncoding transcript
Transcript: ENSMUST00000195868
|
| Coding Region Coverage |
|
| Validation Efficiency |
|
| MGI Phenotype |
FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] The calpains, calcium-activated neutral proteases, are nonlysosomal, intracellular cysteine proteases. The mammalian calpains include ubiquitous, stomach-specific, and muscle-specific proteins. The ubiquitous enzymes consist of heterodimers with distinct large, catalytic subunits associated with a common small, regulatory subunit. This gene encodes the large subunit of the ubiquitous enzyme, calpain 2. Multiple heterogeneous transcriptional start sites in the 5' UTR have been reported. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2009]
PHENOTYPE: Homozygous inactivation of this gene leads to complete prenatal lethality. Mice homozygous for one null allele display placental dysfunction, thin ventricular walls, and peripheral vessel failure. [provided by MGI curators]
|
| Allele List at MGI |
All alleles(1) : Targeted, knock-out(1) |
| Other mutations in this stock |
Total: 19 list
| Gene | Ref | Var | Chr/Loc | Mutation | Predicted Effect | Zygosity |
|---|
| Adgrv1 |
C |
T |
13: 81,255,017 (GRCm39) |
|
probably null |
Het |
| Agt |
A |
G |
8: 125,286,107 (GRCm39) |
F325S |
probably damaging |
Het |
| Arnt2 |
T |
C |
7: 83,993,042 (GRCm39) |
E166G |
possibly damaging |
Het |
| Bltp1 |
T |
C |
3: 37,004,145 (GRCm39) |
I1619T |
possibly damaging |
Het |
| Cacna1h |
T |
C |
17: 25,610,307 (GRCm39) |
D733G |
probably damaging |
Het |
| Cd74 |
A |
G |
18: 60,944,996 (GRCm39) |
|
probably benign |
Het |
| Eno4 |
T |
C |
19: 58,941,637 (GRCm39) |
L185P |
probably benign |
Het |
| Exoc5 |
G |
A |
14: 49,270,832 (GRCm39) |
P398S |
probably benign |
Het |
| Fsd2 |
T |
C |
7: 81,208,918 (GRCm39) |
M187V |
probably benign |
Het |
| Lrfn5 |
C |
T |
12: 61,890,112 (GRCm39) |
T467M |
probably damaging |
Het |
| Mrgpra9 |
A |
G |
7: 46,885,385 (GRCm39) |
I94T |
possibly damaging |
Het |
| Nuak1 |
A |
C |
10: 84,275,993 (GRCm39) |
F77V |
possibly damaging |
Het |
| Nudt9 |
T |
C |
5: 104,206,092 (GRCm39) |
|
probably benign |
Het |
| Or5ar1 |
A |
G |
2: 85,671,317 (GRCm39) |
W273R |
probably benign |
Het |
| Ppp3cc |
T |
A |
14: 70,485,031 (GRCm39) |
I208F |
probably damaging |
Het |
| Prrc1 |
G |
A |
18: 57,522,389 (GRCm39) |
R407Q |
probably benign |
Het |
| Ror1 |
G |
A |
4: 100,265,142 (GRCm39) |
|
probably null |
Het |
| Tmem161b |
T |
A |
13: 84,442,714 (GRCm39) |
|
probably benign |
Het |
| Wdr35 |
C |
A |
12: 9,039,936 (GRCm39) |
A347E |
probably damaging |
Het |
|
| Other mutations in Capn2 |
| Allele | Source | Chr | Coord | Type | Predicted Effect | PPH Score |
|---|
|
IGL02049:Capn2
|
APN |
1 |
182,301,519 (GRCm39) |
splice site |
probably benign |
|
|
IGL02589:Capn2
|
APN |
1 |
182,311,913 (GRCm39) |
missense |
probably damaging |
1.00 |
|
IGL02679:Capn2
|
APN |
1 |
182,300,149 (GRCm39) |
missense |
probably benign |
|
|
E7848:Capn2
|
UTSW |
1 |
182,314,159 (GRCm39) |
missense |
possibly damaging |
0.67 |
|
R0070:Capn2
|
UTSW |
1 |
182,301,434 (GRCm39) |
splice site |
probably benign |
|
|
R0070:Capn2
|
UTSW |
1 |
182,301,434 (GRCm39) |
splice site |
probably benign |
|
|
R0540:Capn2
|
UTSW |
1 |
182,319,749 (GRCm39) |
nonsense |
probably null |
|
|
R0571:Capn2
|
UTSW |
1 |
182,298,325 (GRCm39) |
missense |
probably benign |
0.01 |
|
R1620:Capn2
|
UTSW |
1 |
182,344,702 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R1818:Capn2
|
UTSW |
1 |
182,300,162 (GRCm39) |
missense |
probably benign |
0.00 |
|
R1819:Capn2
|
UTSW |
1 |
182,300,162 (GRCm39) |
missense |
probably benign |
0.00 |
|
R1822:Capn2
|
UTSW |
1 |
182,300,525 (GRCm39) |
missense |
possibly damaging |
0.95 |
|
R1880:Capn2
|
UTSW |
1 |
182,316,581 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R2174:Capn2
|
UTSW |
1 |
182,307,290 (GRCm39) |
missense |
probably benign |
0.22 |
|
R2391:Capn2
|
UTSW |
1 |
182,306,174 (GRCm39) |
missense |
probably benign |
0.01 |
|
R2860:Capn2
|
UTSW |
1 |
182,300,485 (GRCm39) |
splice site |
probably benign |
|
|
R2861:Capn2
|
UTSW |
1 |
182,300,485 (GRCm39) |
splice site |
probably benign |
|
|
R2878:Capn2
|
UTSW |
1 |
182,344,798 (GRCm39) |
missense |
probably benign |
0.00 |
|
R3052:Capn2
|
UTSW |
1 |
182,315,337 (GRCm39) |
missense |
probably benign |
0.06 |
|
R4463:Capn2
|
UTSW |
1 |
182,307,329 (GRCm39) |
intron |
probably benign |
|
|
R4669:Capn2
|
UTSW |
1 |
182,298,345 (GRCm39) |
missense |
probably benign |
0.00 |
|
R5077:Capn2
|
UTSW |
1 |
182,300,138 (GRCm39) |
missense |
possibly damaging |
0.71 |
|
R5397:Capn2
|
UTSW |
1 |
182,298,271 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R5696:Capn2
|
UTSW |
1 |
182,306,165 (GRCm39) |
missense |
possibly damaging |
0.79 |
|
R6777:Capn2
|
UTSW |
1 |
182,297,742 (GRCm39) |
critical splice donor site |
probably null |
|
|
R6800:Capn2
|
UTSW |
1 |
182,309,045 (GRCm39) |
missense |
probably damaging |
0.99 |
|
R7741:Capn2
|
UTSW |
1 |
182,307,288 (GRCm39) |
nonsense |
probably null |
|
|
R7814:Capn2
|
UTSW |
1 |
182,319,711 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R7995:Capn2
|
UTSW |
1 |
182,306,111 (GRCm39) |
critical splice donor site |
probably null |
|
|
R8223:Capn2
|
UTSW |
1 |
182,310,099 (GRCm39) |
critical splice donor site |
probably null |
|
|
R8446:Capn2
|
UTSW |
1 |
182,311,796 (GRCm39) |
missense |
possibly damaging |
0.90 |
|
R8496:Capn2
|
UTSW |
1 |
182,304,840 (GRCm39) |
missense |
probably benign |
0.04 |
|
R9623:Capn2
|
UTSW |
1 |
182,344,795 (GRCm39) |
missense |
probably benign |
0.05 |
|
| Posted On |
2016-08-02 |
Incidental Mutation