Incidental Mutation 'R0466:Gcg'
ID 41619
Institutional Source Beutler Lab
Gene Symbol Gcg
Ensembl Gene ENSMUSG00000000394
Gene Name glucagon
Synonyms Glu, GLP-1, glucagon-like peptide I, PPG
MMRRC Submission 038666-MU
Accession Numbers
Essential gene? Probably non essential (E-score: 0.149) question?
Stock # R0466 (G1)
Quality Score 225
Status Validated
Chromosome 2
Chromosomal Location 62304874-62313994 bp(-) (GRCm39)
Type of Mutation missense
DNA Base Change (assembly) T to C at 62307282 bp (GRCm39)
Zygosity Heterozygous
Amino Acid Change Aspartic acid to Glycine at position 93 (D93G)
Ref Sequence ENSEMBL: ENSMUSP00000099794 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000102733] [ENSMUST00000136686]
AlphaFold P55095
Predicted Effect probably damaging
Transcript: ENSMUST00000102733
AA Change: D93G

PolyPhen 2 Score 0.991 (Sensitivity: 0.71; Specificity: 0.97)
SMART Domains Protein: ENSMUSP00000099794
Gene: ENSMUSG00000000394
AA Change: D93G

DomainStartEndE-ValueType
signal peptide 1 17 N/A INTRINSIC
GLUCA 53 79 7.5e-15 SMART
GLUCA 98 124 4.06e-11 SMART
GLUCA 146 172 1.97e-9 SMART
Predicted Effect possibly damaging
Transcript: ENSMUST00000136686
AA Change: D93G

PolyPhen 2 Score 0.514 (Sensitivity: 0.88; Specificity: 0.90)
SMART Domains Protein: ENSMUSP00000115892
Gene: ENSMUSG00000000394
AA Change: D93G

DomainStartEndE-ValueType
signal peptide 1 17 N/A INTRINSIC
GLUCA 53 79 7.5e-15 SMART
GLUCA 98 124 4.06e-11 SMART
GLUCA 146 172 1.97e-9 SMART
Predicted Effect noncoding transcript
Transcript: ENSMUST00000155624
Meta Mutation Damage Score 0.0641 question?
Coding Region Coverage
  • 1x: 99.2%
  • 3x: 98.5%
  • 10x: 96.8%
  • 20x: 94.1%
Validation Efficiency 97% (63/65)
MGI Phenotype FUNCTION: This gene encodes glucagon, a pancreatic hormone that counteracts the action of insulin in the bloodstream. The encoded protein is processed to generate glucagon and two other glucagon-like peptides, GLP1 and GLP2. Glucagon stimulates gluconeogenesis, glycogenolysis and lipolysis. GLP1 induces secretion of insulin, suppresses glucagon secretion and inhibits feeding. GLP2 induces intestinal absorption of glucose by stimulating the growth of intestinal cells and preventing apoptosis. [provided by RefSeq, Apr 2015]
PHENOTYPE: Mice homozygous for a knock-out allele exhibit islet alpha cell hyperplasia and increased body weight. [provided by MGI curators]
Allele List at MGI
Other mutations in this stock
Total: 60 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
4933412E24Rik T C 15: 59,887,321 (GRCm39) Y373C probably benign Het
Abca12 T G 1: 71,341,822 (GRCm39) Q1046H probably damaging Het
Adgrv1 A G 13: 81,714,415 (GRCm39) F956S probably benign Het
Alk A G 17: 72,212,152 (GRCm39) V797A possibly damaging Het
Ascl2 A G 7: 142,522,217 (GRCm39) L77P probably benign Het
Aspm A T 1: 139,405,639 (GRCm39) I1509F probably damaging Het
AY358078 A T 14: 52,043,089 (GRCm39) Y259F unknown Het
Cbs G A 17: 31,835,126 (GRCm39) A450V probably benign Het
Cdh11 T A 8: 103,396,690 (GRCm39) Q213L possibly damaging Het
Cdh26 C T 2: 178,123,425 (GRCm39) R675C possibly damaging Het
Cfap126 T C 1: 170,953,769 (GRCm39) I113T probably damaging Het
Clk4 A G 11: 51,158,155 (GRCm39) D53G possibly damaging Het
Dab1 T C 4: 104,577,747 (GRCm39) L272P probably benign Het
Dmtf1 A T 5: 9,182,454 (GRCm39) probably null Het
Dph5 A C 3: 115,722,359 (GRCm39) D279A probably benign Het
Fbxw19 T A 9: 109,307,717 (GRCm39) T461S probably benign Het
G3bp1 T C 11: 55,389,452 (GRCm39) F383L probably damaging Het
Gmps A G 3: 63,901,365 (GRCm39) T395A probably damaging Het
H2-Ob A G 17: 34,461,633 (GRCm39) D124G probably damaging Het
Itga8 G T 2: 12,237,697 (GRCm39) A341E probably damaging Het
Itih3 A G 14: 30,634,831 (GRCm39) probably null Het
Kcnh4 C T 11: 100,637,758 (GRCm39) G633E probably benign Het
Kif2c C T 4: 117,029,489 (GRCm39) R215Q possibly damaging Het
Letm1 A C 5: 33,919,074 (GRCm39) probably benign Het
Lypd8l T C 11: 58,503,331 (GRCm39) probably benign Het
Mmp3 A G 9: 7,450,165 (GRCm39) D299G probably damaging Het
Myh8 G T 11: 67,189,405 (GRCm39) A1194S probably benign Het
Naip2 A C 13: 100,298,290 (GRCm39) I582S probably benign Het
Nfib A C 4: 82,416,775 (GRCm39) Y87D probably damaging Het
Nlrp4a T C 7: 26,162,045 (GRCm39) probably benign Het
Nsmce1 A T 7: 125,071,408 (GRCm39) probably benign Het
Odad2 T A 18: 7,286,758 (GRCm39) I158F probably benign Het
Or7g12 T G 9: 18,899,551 (GRCm39) V89G probably benign Het
Or7g27 A T 9: 19,250,475 (GRCm39) T240S probably damaging Het
Patj C A 4: 98,576,393 (GRCm39) Q1193K probably damaging Het
Pcdhb5 G A 18: 37,455,596 (GRCm39) V659M probably damaging Het
Pkd1l3 C G 8: 110,350,281 (GRCm39) D375E possibly damaging Het
Pmis2 T C 7: 30,370,817 (GRCm39) I46V probably benign Het
Ppp2r5e A G 12: 75,509,216 (GRCm39) probably benign Het
Prom2 A G 2: 127,370,709 (GRCm39) F825S probably damaging Het
Rab11fip2 G A 19: 59,894,675 (GRCm39) A524V possibly damaging Het
Rb1cc1 A C 1: 6,333,491 (GRCm39) probably null Het
Rwdd3 G C 3: 120,952,668 (GRCm39) Q180E possibly damaging Het
Sema6a G A 18: 47,423,112 (GRCm39) probably null Het
Sgcg A T 14: 61,459,135 (GRCm39) C265S probably damaging Het
Slc16a3 T C 11: 120,848,878 (GRCm39) S445P possibly damaging Het
Slc22a3 G A 17: 12,677,380 (GRCm39) Q263* probably null Het
Sorcs3 A G 19: 48,736,758 (GRCm39) T694A probably benign Het
Tbc1d15 T C 10: 115,055,077 (GRCm39) K322E probably damaging Het
Tecta G T 9: 42,284,369 (GRCm39) F905L probably benign Het
Tmeff1 A G 4: 48,636,853 (GRCm39) I184V possibly damaging Het
Ttf1 A G 2: 28,955,419 (GRCm39) H261R possibly damaging Het
Ttll6 T A 11: 96,036,417 (GRCm39) L349M probably damaging Het
Ubac2 G A 14: 122,211,031 (GRCm39) V134M probably damaging Het
Ubxn4 G A 1: 128,190,641 (GRCm39) E256K probably benign Het
Vmn2r25 T G 6: 123,829,008 (GRCm39) I89L probably benign Het
Vmn2r6 A C 3: 64,463,723 (GRCm39) F370L probably damaging Het
Vps13b T A 15: 35,445,748 (GRCm39) Y412* probably null Het
Zfp142 A G 1: 74,624,570 (GRCm39) S85P possibly damaging Het
Zfp516 G A 18: 82,975,579 (GRCm39) probably null Het
Other mutations in Gcg
AlleleSourceChrCoordTypePredicted EffectPPH Score
IGL01483:Gcg APN 2 62,310,827 (GRCm39) missense possibly damaging 0.86
IGL02582:Gcg APN 2 62,308,922 (GRCm39) nonsense probably null
R3620:Gcg UTSW 2 62,307,279 (GRCm39) missense probably damaging 0.96
R3621:Gcg UTSW 2 62,307,279 (GRCm39) missense probably damaging 0.96
R4744:Gcg UTSW 2 62,308,975 (GRCm39) missense probably damaging 1.00
R4859:Gcg UTSW 2 62,307,189 (GRCm39) missense probably damaging 0.96
R5807:Gcg UTSW 2 62,306,069 (GRCm39) missense possibly damaging 0.54
R5971:Gcg UTSW 2 62,306,148 (GRCm39) missense probably damaging 1.00
R6138:Gcg UTSW 2 62,306,148 (GRCm39) missense probably damaging 1.00
R7191:Gcg UTSW 2 62,307,183 (GRCm39) missense probably damaging 1.00
R7522:Gcg UTSW 2 62,306,103 (GRCm39) missense probably benign 0.32
R8188:Gcg UTSW 2 62,309,004 (GRCm39) missense probably damaging 0.96
R8746:Gcg UTSW 2 62,305,325 (GRCm39) missense probably damaging 1.00
R9261:Gcg UTSW 2 62,306,408 (GRCm39) intron probably benign
R9273:Gcg UTSW 2 62,306,133 (GRCm39) missense probably null 0.44
U15987:Gcg UTSW 2 62,306,148 (GRCm39) missense probably damaging 1.00
Predicted Primers PCR Primer
(F):5'- GGCAAGCAACTAAGAAACTTGCTCTC -3'
(R):5'- GCCCCTGGAAAAGGAAGGTTTAGTG -3'

Sequencing Primer
(F):5'- GTACAGTTTTTCCCATCGGATAG -3'
(R):5'- TTTCCCCACAAAGTTTCAATCCAG -3'
Posted On 2013-05-23