Mutagenetix > Incidental Mutation

Incidental Mutation 'R0477:Zkscan7'

41858

Institutional Source

Beutler Lab

Gene Symbol

Zkscan7

Ensembl Gene

ENSMUSG00000063488

Gene Name

zinc finger with KRAB and SCAN domains 7

Synonyms

Zfp167

MMRRC Submission

038677-MU

Accession Numbers

Essential gene?

Non essential (E-score: 0.000) question?

Stock #

R0477 (G1)

Quality Score

225

Status

Validated (trace)

Chromosome

Chromosomal Location

122715883-122727684 bp(+) (GRCm39)

Type of Mutation

splice site (3 bp from exon)

DNA Base Change (assembly)

A to G at 122719874 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Ref Sequence

ENSEMBL: ENSMUSP00000150648 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000063980] [ENSMUST00000215872]

AlphaFold

E9PVW1

Predicted Effect

probably null
Transcript: ENSMUST00000063980

SMART Domains

Protein: ENSMUSP00000071695
Gene: ENSMUSG00000063488

Domain	Start	End	E-Value	Type
SCAN	45	156	1.18e-65	SMART
ZnF_C2H2	350	372	5.59e-4	SMART
ZnF_C2H2	378	400	3.69e-4	SMART
ZnF_C2H2	434	456	8.4e1	SMART
ZnF_C2H2	487	509	4.24e-4	SMART
ZnF_C2H2	515	537	8.34e-3	SMART
ZnF_C2H2	543	565	7.37e-4	SMART
ZnF_C2H2	571	593	1.92e-2	SMART
ZnF_C2H2	599	621	1.13e-4	SMART
ZnF_C2H2	627	649	2.24e-3	SMART
ZnF_C2H2	655	677	4.17e-3	SMART
ZnF_C2H2	683	705	1.04e-3	SMART

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000180486

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000180877

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000214426

Predicted Effect

probably null
Transcript: ENSMUST00000215872

Meta Mutation Damage Score

0.9755

Coding Region Coverage

1x: 99.2%
3x: 98.4%
10x: 96.4%
20x: 93.1%

Validation Efficiency

98% (57/58)

Allele List at MGI

Other mutations in this stock

Total: 55 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
6030468B19Rik	A	T	11: 117,693,787 (GRCm39)	I85F	probably benign	Het
Abca8a	T	A	11: 109,956,051 (GRCm39)	I778L	probably benign	Het
Abcc5	T	C	16: 20,187,319 (GRCm39)	N889S	possibly damaging	Het
Abcc5	T	C	16: 20,217,635 (GRCm39)	N359D	probably damaging	Het
Adam23	A	G	1: 63,596,559 (GRCm39)		probably benign	Het
Adamts3	A	T	5: 89,832,366 (GRCm39)	D913E	probably benign	Het
Ap1b1	G	T	11: 4,981,787 (GRCm39)	C538F	probably benign	Het
Ash1l	T	A	3: 88,890,766 (GRCm39)	S882T	probably benign	Het
C9	A	T	15: 6,487,664 (GRCm39)	E43D	probably benign	Het
Cacna2d1	T	C	5: 16,399,796 (GRCm39)		probably null	Het
Ces2a	A	G	8: 105,464,169 (GRCm39)	E267G	probably damaging	Het
Cfap61	A	G	2: 145,781,836 (GRCm39)	D23G	probably damaging	Het
Col9a3	T	G	2: 180,251,263 (GRCm39)		probably benign	Het
Cstl1	T	C	2: 148,592,908 (GRCm39)	V21A	probably benign	Het
Cth	A	T	3: 157,610,812 (GRCm39)	L340Q	probably damaging	Het
Dnah8	T	A	17: 30,974,054 (GRCm39)	M2813K	probably damaging	Het
Fam107a	A	T	14: 8,301,168 (GRCm38)	Y21N	probably benign	Het
Fam184a	G	A	10: 53,531,175 (GRCm39)	T733M	probably damaging	Het
Fer1l4	A	G	2: 155,894,806 (GRCm39)	V21A	probably benign	Het
Foxc2	A	T	8: 121,844,774 (GRCm39)	Y474F	probably damaging	Het
Hnf4g	G	T	3: 3,716,851 (GRCm39)		probably benign	Het
Hnrnpll	T	C	17: 80,369,261 (GRCm39)	D54G	unknown	Het
Hydin	A	G	8: 111,145,130 (GRCm39)	Y827C	probably damaging	Het
Il23r	A	G	6: 67,429,361 (GRCm39)	V327A	probably benign	Het
Itih4	T	A	14: 30,611,631 (GRCm39)	V118D	probably damaging	Het
Kmt2d	G	A	15: 98,751,462 (GRCm39)		probably benign	Het
Lamb1	A	G	12: 31,376,268 (GRCm39)	D1546G	possibly damaging	Het
Large1	A	T	8: 73,544,710 (GRCm39)	D689E	probably damaging	Het
Map1a	T	C	2: 121,132,582 (GRCm39)	S895P	probably damaging	Het
Mdn1	A	C	4: 32,750,928 (GRCm39)	E4487A	probably benign	Het
Myo15a	T	C	11: 60,411,740 (GRCm39)		probably null	Het
Nlrp4f	C	A	13: 65,338,720 (GRCm39)	R639L	probably benign	Het
Or4f52	A	G	2: 111,062,009 (GRCm39)	F43S	probably benign	Het
Or8h10	T	A	2: 86,808,567 (GRCm39)	D191V	probably damaging	Het
Pcdh9	T	C	14: 94,125,114 (GRCm39)	N229S	probably damaging	Het
Pcnx2	A	G	8: 126,488,306 (GRCm39)	V1746A	probably damaging	Het
Phf12	A	T	11: 77,913,896 (GRCm39)	H446L	possibly damaging	Het
Phlpp2	A	G	8: 110,622,138 (GRCm39)		probably null	Het
Psmb9	A	C	17: 34,401,238 (GRCm39)	V207G	probably damaging	Het
Ptprh	C	A	7: 4,600,997 (GRCm39)	D127Y	possibly damaging	Het
Rabep1	T	G	11: 70,811,733 (GRCm39)	M535R	probably damaging	Het
Rif1	GCCACCA	GCCA	2: 52,000,336 (GRCm39)		probably benign	Het
Scin	T	C	12: 40,110,515 (GRCm39)	D711G	probably damaging	Het
Slfn4	T	C	11: 83,079,507 (GRCm39)	I6T	probably benign	Het
Sos1	T	A	17: 80,742,363 (GRCm39)	E388V	possibly damaging	Het
Spag5	A	C	11: 78,205,024 (GRCm39)	Q603P	probably damaging	Het
Supv3l1	G	T	10: 62,266,364 (GRCm39)	T604N	probably damaging	Het
Tbx5	A	G	5: 120,021,184 (GRCm39)	S397G	possibly damaging	Het
Tmprss5	A	G	9: 49,026,465 (GRCm39)	D383G	possibly damaging	Het
Trim43b	A	G	9: 88,972,654 (GRCm39)	W167R	probably damaging	Het
Unc80	A	T	1: 66,609,160 (GRCm39)	D1283V	probably damaging	Het
Upf1	A	T	8: 70,786,730 (GRCm39)	V918D	probably benign	Het
Vmn2r100	A	G	17: 19,742,776 (GRCm39)	I383M	probably benign	Het
Zc3h3	G	T	15: 75,648,932 (GRCm39)	S733R	possibly damaging	Het
Zcchc2	C	T	1: 105,958,000 (GRCm39)	P426S	possibly damaging	Het

Other mutations in Zkscan7

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00594:Zkscan7	APN	9	122,724,659 (GRCm39)	missense	possibly damaging	0.95
IGL01650:Zkscan7	APN	9	122,723,892 (GRCm39)	missense	probably benign
IGL01905:Zkscan7	APN	9	122,719,826 (GRCm39)	missense	possibly damaging	0.93
IGL02466:Zkscan7	APN	9	122,717,950 (GRCm39)	missense	probably damaging	0.98
R0310:Zkscan7	UTSW	9	122,717,958 (GRCm39)	nonsense	probably null
R0355:Zkscan7	UTSW	9	122,717,872 (GRCm39)	missense	probably damaging	1.00
R1276:Zkscan7	UTSW	9	122,719,788 (GRCm39)	missense	probably damaging	0.98
R1426:Zkscan7	UTSW	9	122,724,228 (GRCm39)	missense	probably benign
R2055:Zkscan7	UTSW	9	122,718,002 (GRCm39)	missense	probably damaging	1.00
R2195:Zkscan7	UTSW	9	122,724,686 (GRCm39)	missense	possibly damaging	0.73
R2354:Zkscan7	UTSW	9	122,723,892 (GRCm39)	missense	probably benign
R4878:Zkscan7	UTSW	9	122,719,865 (GRCm39)	nonsense	probably null
R5106:Zkscan7	UTSW	9	122,725,198 (GRCm39)	unclassified	probably benign
R6266:Zkscan7	UTSW	9	122,724,299 (GRCm39)	nonsense	probably null
R6299:Zkscan7	UTSW	9	122,717,782 (GRCm39)	missense	probably damaging	1.00
R6513:Zkscan7	UTSW	9	122,725,170 (GRCm39)	missense	probably benign	0.00
R6881:Zkscan7	UTSW	9	122,717,766 (GRCm39)	missense	possibly damaging	0.96
R7640:Zkscan7	UTSW	9	122,725,121 (GRCm39)	missense	possibly damaging	0.71
R7920:Zkscan7	UTSW	9	122,724,974 (GRCm39)	missense	probably benign	0.19

Predicted Primers

PCR Primer
(F):5'- TACACACTTGTGCCGATGCACC -3'
(R):5'- AGACTGCTCTGGCTTGAGGGAAAC -3'

Sequencing Primer
(F):5'- TCTAGCTCCTACAAGGAGAGTCTG -3'
(R):5'- GGGAAACTTGGTAAGTACATGC -3'

Posted On

2013-05-23