Incidental Mutation 'R5340:Zfp667'
| ID |
422279 |
| Institutional Source |
Beutler Lab
|
| Gene Symbol |
Zfp667
|
| Ensembl Gene |
ENSMUSG00000054893 |
| Gene Name |
zinc finger protein 667 |
| Synonyms |
A830025F02Rik |
| MMRRC Submission |
042919-MU
|
| Accession Numbers |
|
| Essential gene? |
Probably non essential
(E-score: 0.073)
|
| Stock # |
R5340 (G1)
|
| Quality Score |
225 |
|
Status
|
Not validated
|
| Chromosome |
7 |
| Chromosomal Location |
6289578-6310882 bp(+) (GRCm39) |
| Type of Mutation |
missense |
| DNA Base Change (assembly) |
A to G
at 6308252 bp (GRCm39)
|
| Zygosity |
Heterozygous |
| Amino Acid Change |
Threonine to Alanine
at position 307
(T307A)
|
| Ref Sequence |
ENSEMBL: ENSMUSP00000128658
(fasta)
|
| Gene Model |
predicted gene model for transcript(s):
[ENSMUST00000086327]
[ENSMUST00000108562]
[ENSMUST00000153840]
[ENSMUST00000170776]
|
| AlphaFold |
Q2TL60 |
| Predicted Effect |
possibly damaging
Transcript: ENSMUST00000086327
AA Change: T307A
PolyPhen 2
Score 0.528 (Sensitivity: 0.88; Specificity: 0.90)
|
| SMART Domains |
Protein: ENSMUSP00000083507
Gene: ENSMUSG00000054893
AA Change: T307A
| Domain | Start | End | E-Value | Type |
|---|
|
KRAB
|
14 |
74 |
4.77e-30 |
SMART |
|
ZnF_C2H2
|
144 |
166 |
5.42e-2 |
SMART |
|
ZnF_C2H2
|
172 |
194 |
3.11e-2 |
SMART |
|
ZnF_C2H2
|
200 |
222 |
1.67e-2 |
SMART |
|
ZnF_C2H2
|
253 |
275 |
2.57e-3 |
SMART |
|
ZnF_C2H2
|
329 |
351 |
2.4e-3 |
SMART |
|
ZnF_C2H2
|
357 |
379 |
3.16e-3 |
SMART |
|
ZnF_C2H2
|
385 |
407 |
8.94e-3 |
SMART |
|
ZnF_C2H2
|
414 |
436 |
5.06e-2 |
SMART |
|
ZnF_C2H2
|
442 |
464 |
2.4e-3 |
SMART |
|
ZnF_C2H2
|
470 |
492 |
5.29e-5 |
SMART |
|
ZnF_C2H2
|
498 |
520 |
7.37e-4 |
SMART |
|
ZnF_C2H2
|
526 |
548 |
1.38e-3 |
SMART |
|
ZnF_C2H2
|
554 |
576 |
1.13e-4 |
SMART |
|
ZnF_C2H2
|
582 |
604 |
1.38e-3 |
SMART |
|
| Predicted Effect |
possibly damaging
Transcript: ENSMUST00000108562
AA Change: T307A
PolyPhen 2
Score 0.528 (Sensitivity: 0.88; Specificity: 0.90)
|
| SMART Domains |
Protein: ENSMUSP00000104202
Gene: ENSMUSG00000054893
AA Change: T307A
| Domain | Start | End | E-Value | Type |
|---|
|
KRAB
|
14 |
74 |
4.77e-30 |
SMART |
|
ZnF_C2H2
|
144 |
166 |
5.42e-2 |
SMART |
|
ZnF_C2H2
|
172 |
194 |
3.11e-2 |
SMART |
|
ZnF_C2H2
|
200 |
222 |
1.67e-2 |
SMART |
|
ZnF_C2H2
|
253 |
275 |
2.57e-3 |
SMART |
|
ZnF_C2H2
|
329 |
351 |
2.4e-3 |
SMART |
|
ZnF_C2H2
|
357 |
379 |
3.16e-3 |
SMART |
|
ZnF_C2H2
|
385 |
407 |
8.94e-3 |
SMART |
|
ZnF_C2H2
|
414 |
436 |
5.06e-2 |
SMART |
|
ZnF_C2H2
|
442 |
464 |
2.4e-3 |
SMART |
|
ZnF_C2H2
|
470 |
492 |
5.29e-5 |
SMART |
|
ZnF_C2H2
|
498 |
520 |
7.37e-4 |
SMART |
|
ZnF_C2H2
|
526 |
548 |
1.38e-3 |
SMART |
|
ZnF_C2H2
|
554 |
576 |
1.13e-4 |
SMART |
|
ZnF_C2H2
|
582 |
604 |
1.38e-3 |
SMART |
|
| Predicted Effect |
probably benign
Transcript: ENSMUST00000153840
|
| Predicted Effect |
possibly damaging
Transcript: ENSMUST00000170776
AA Change: T307A
PolyPhen 2
Score 0.528 (Sensitivity: 0.88; Specificity: 0.90)
|
| SMART Domains |
Protein: ENSMUSP00000128658
Gene: ENSMUSG00000054893
AA Change: T307A
| Domain | Start | End | E-Value | Type |
|---|
|
KRAB
|
14 |
74 |
4.77e-30 |
SMART |
|
ZnF_C2H2
|
144 |
166 |
5.42e-2 |
SMART |
|
ZnF_C2H2
|
172 |
194 |
3.11e-2 |
SMART |
|
ZnF_C2H2
|
200 |
222 |
1.67e-2 |
SMART |
|
ZnF_C2H2
|
253 |
275 |
2.57e-3 |
SMART |
|
ZnF_C2H2
|
329 |
351 |
2.4e-3 |
SMART |
|
ZnF_C2H2
|
357 |
379 |
3.16e-3 |
SMART |
|
ZnF_C2H2
|
385 |
407 |
8.94e-3 |
SMART |
|
ZnF_C2H2
|
414 |
436 |
5.06e-2 |
SMART |
|
ZnF_C2H2
|
442 |
464 |
2.4e-3 |
SMART |
|
ZnF_C2H2
|
470 |
492 |
5.29e-5 |
SMART |
|
ZnF_C2H2
|
498 |
520 |
7.37e-4 |
SMART |
|
ZnF_C2H2
|
526 |
548 |
1.38e-3 |
SMART |
|
ZnF_C2H2
|
554 |
576 |
1.13e-4 |
SMART |
|
ZnF_C2H2
|
582 |
604 |
1.38e-3 |
SMART |
|
| Coding Region Coverage |
- 1x: 99.4%
- 3x: 98.8%
- 10x: 97.7%
- 20x: 96.3%
|
| Validation Efficiency |
|
| Allele List at MGI |
All alleles(4) : Targeted, other(2) Gene trapped(2)
|
| Other mutations in this stock |
Total: 44 list
| Gene | Ref | Var | Chr/Loc | Mutation | Predicted Effect | Zygosity |
|---|
| Aqp7 |
T |
C |
4: 41,034,347 (GRCm39) |
R271G |
probably benign |
Het |
| Atp4a |
A |
G |
7: 30,420,231 (GRCm39) |
I793V |
probably benign |
Het |
| Baz2a |
C |
T |
10: 127,950,911 (GRCm39) |
R535C |
probably damaging |
Het |
| BC034090 |
T |
C |
1: 155,102,160 (GRCm39) |
T35A |
possibly damaging |
Het |
| Bnip1 |
T |
C |
17: 27,005,764 (GRCm39) |
|
probably null |
Het |
| Ccdc136 |
A |
G |
6: 29,411,859 (GRCm39) |
S369G |
possibly damaging |
Het |
| Csrnp3 |
A |
G |
2: 65,852,781 (GRCm39) |
D391G |
probably benign |
Het |
| Ctbp2 |
G |
T |
7: 132,615,692 (GRCm39) |
H414Q |
probably benign |
Het |
| Dnah6 |
A |
G |
6: 73,189,603 (GRCm39) |
I15T |
probably benign |
Het |
| Dync2li1 |
A |
G |
17: 84,957,130 (GRCm39) |
|
probably null |
Het |
| Eri3 |
A |
T |
4: 117,530,991 (GRCm39) |
I329F |
probably damaging |
Het |
| Flii |
T |
A |
11: 60,608,094 (GRCm39) |
I786F |
probably damaging |
Het |
| Fmo1 |
A |
T |
1: 162,657,551 (GRCm39) |
I530N |
probably benign |
Het |
| Gm28042 |
T |
C |
2: 119,871,929 (GRCm39) |
S960P |
probably benign |
Het |
| Gzmm |
C |
A |
10: 79,530,907 (GRCm39) |
F236L |
probably benign |
Het |
| H2bl1 |
C |
T |
13: 99,120,951 (GRCm39) |
R25H |
possibly damaging |
Het |
| Igkv4-55 |
A |
G |
6: 69,584,489 (GRCm39) |
V41A |
probably damaging |
Het |
| Ipo9 |
A |
G |
1: 135,313,170 (GRCm39) |
Y1020H |
probably benign |
Het |
| Masp1 |
T |
C |
16: 23,276,858 (GRCm39) |
Y549C |
probably damaging |
Het |
| Mblac1 |
A |
T |
5: 138,192,840 (GRCm39) |
S61C |
probably damaging |
Het |
| Mical1 |
T |
A |
10: 41,359,427 (GRCm39) |
|
probably null |
Het |
| Mroh9 |
C |
T |
1: 162,908,156 (GRCm39) |
|
probably benign |
Het |
| Mta2 |
T |
C |
19: 8,919,720 (GRCm39) |
M1T |
probably null |
Het |
| Neb |
A |
G |
2: 52,113,060 (GRCm39) |
Y4245H |
probably damaging |
Het |
| Or11j4 |
T |
C |
14: 50,630,677 (GRCm39) |
F155L |
probably damaging |
Het |
| Or4c52 |
T |
C |
2: 89,845,706 (GRCm39) |
V144A |
probably benign |
Het |
| Otulinl |
T |
C |
15: 27,658,175 (GRCm39) |
M282V |
possibly damaging |
Het |
| Pak2 |
G |
T |
16: 31,853,764 (GRCm39) |
|
probably null |
Het |
| Phc3 |
A |
T |
3: 30,961,616 (GRCm39) |
F939I |
probably damaging |
Het |
| Prss3l |
T |
C |
6: 41,422,307 (GRCm39) |
N33D |
probably benign |
Het |
| Rogdi |
C |
A |
16: 4,831,225 (GRCm39) |
R14L |
probably benign |
Het |
| Ryr3 |
T |
C |
2: 112,664,470 (GRCm39) |
Y1627C |
probably damaging |
Het |
| Scrn3 |
A |
T |
2: 73,166,154 (GRCm39) |
K396* |
probably null |
Het |
| Sh3pxd2a |
T |
C |
19: 47,256,670 (GRCm39) |
N683D |
probably benign |
Het |
| Smtnl1 |
T |
A |
2: 84,645,785 (GRCm39) |
H362L |
probably damaging |
Het |
| Sned1 |
A |
G |
1: 93,210,479 (GRCm39) |
S927G |
probably benign |
Het |
| Tcaf1 |
A |
G |
6: 42,655,923 (GRCm39) |
V351A |
probably damaging |
Het |
| Tmem232 |
C |
T |
17: 65,709,993 (GRCm39) |
V432M |
possibly damaging |
Het |
| Ttll11 |
G |
T |
2: 35,792,801 (GRCm39) |
H347Q |
probably damaging |
Het |
| Vmn2r56 |
A |
C |
7: 12,449,799 (GRCm39) |
D146E |
probably damaging |
Het |
| Wwp1 |
A |
G |
4: 19,638,773 (GRCm39) |
|
probably null |
Het |
| Zcchc7 |
T |
A |
4: 44,762,245 (GRCm39) |
N124K |
probably benign |
Het |
| Zfp709 |
T |
A |
8: 72,643,596 (GRCm39) |
C342S |
probably damaging |
Het |
| Zfp940 |
A |
G |
7: 29,544,266 (GRCm39) |
V547A |
probably benign |
Het |
|
| Other mutations in Zfp667 |
| Allele | Source | Chr | Coord | Type | Predicted Effect | PPH Score |
|---|
|
IGL00821:Zfp667
|
APN |
7 |
6,308,396 (GRCm39) |
missense |
possibly damaging |
0.53 |
|
IGL01325:Zfp667
|
APN |
7 |
6,293,545 (GRCm39) |
missense |
probably damaging |
1.00 |
|
IGL01386:Zfp667
|
APN |
7 |
6,307,869 (GRCm39) |
missense |
probably benign |
0.00 |
|
IGL01960:Zfp667
|
APN |
7 |
6,308,336 (GRCm39) |
missense |
probably benign |
0.00 |
|
IGL03394:Zfp667
|
APN |
7 |
6,292,438 (GRCm39) |
critical splice donor site |
probably null |
|
|
B5639:Zfp667
|
UTSW |
7 |
6,293,544 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R0458:Zfp667
|
UTSW |
7 |
6,307,844 (GRCm39) |
missense |
probably benign |
0.40 |
|
R0845:Zfp667
|
UTSW |
7 |
6,309,091 (GRCm39) |
missense |
possibly damaging |
0.85 |
|
R1768:Zfp667
|
UTSW |
7 |
6,308,066 (GRCm39) |
missense |
possibly damaging |
0.53 |
|
R1953:Zfp667
|
UTSW |
7 |
6,308,087 (GRCm39) |
missense |
probably benign |
0.04 |
|
R2023:Zfp667
|
UTSW |
7 |
6,308,416 (GRCm39) |
missense |
possibly damaging |
0.85 |
|
R3159:Zfp667
|
UTSW |
7 |
6,308,999 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R4080:Zfp667
|
UTSW |
7 |
6,308,105 (GRCm39) |
missense |
possibly damaging |
0.71 |
|
R4476:Zfp667
|
UTSW |
7 |
6,307,598 (GRCm39) |
missense |
possibly damaging |
0.53 |
|
R4584:Zfp667
|
UTSW |
7 |
6,293,624 (GRCm39) |
missense |
possibly damaging |
0.84 |
|
R4783:Zfp667
|
UTSW |
7 |
6,308,684 (GRCm39) |
missense |
possibly damaging |
0.83 |
|
R5037:Zfp667
|
UTSW |
7 |
6,308,949 (GRCm39) |
missense |
possibly damaging |
0.71 |
|
R5300:Zfp667
|
UTSW |
7 |
6,307,635 (GRCm39) |
missense |
probably benign |
|
|
R5311:Zfp667
|
UTSW |
7 |
6,308,715 (GRCm39) |
missense |
probably benign |
0.10 |
|
R5312:Zfp667
|
UTSW |
7 |
6,308,466 (GRCm39) |
missense |
probably benign |
|
|
R6262:Zfp667
|
UTSW |
7 |
6,307,973 (GRCm39) |
missense |
probably benign |
0.03 |
|
R7386:Zfp667
|
UTSW |
7 |
6,308,949 (GRCm39) |
missense |
possibly damaging |
0.86 |
|
R8383:Zfp667
|
UTSW |
7 |
6,308,370 (GRCm39) |
missense |
probably damaging |
0.98 |
|
R8919:Zfp667
|
UTSW |
7 |
6,308,256 (GRCm39) |
missense |
possibly damaging |
0.53 |
|
R9099:Zfp667
|
UTSW |
7 |
6,308,322 (GRCm39) |
missense |
probably benign |
0.00 |
|
R9422:Zfp667
|
UTSW |
7 |
6,308,321 (GRCm39) |
missense |
probably benign |
0.00 |
|
Z1177:Zfp667
|
UTSW |
7 |
6,307,856 (GRCm39) |
missense |
possibly damaging |
0.91 |
|
| Predicted Primers |
PCR Primer
(F):5'- CTACTGACAGCGTTCACCAG -3'
(R):5'- GGATGAAAGGCGACTACACACC -3'
Sequencing Primer
(F):5'- TTCACCAGTGCAGGAAGTGTG -3'
(R):5'- GAGAGCCGTCCAAAGTCTTTCTG -3'
|
| Posted On |
2016-08-04 |
Incidental Mutation