Mutagenetix > Incidental Mutation

Incidental Mutation 'R5361:Or6c66'

422816

Institutional Source

Beutler Lab

Gene Symbol

Or6c66

Ensembl Gene

ENSMUSG00000095002

Gene Name

olfactory receptor family 6 subfamily C member 66

Synonyms

GA_x6K02T2PULF-11304679-11303744, MOR108-1, Olfr798

MMRRC Submission

042940-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.191) question?

Stock #

R5361 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

129460993-129461928 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to T at 129461601 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Phenylalanine to Isoleucine at position 110 (F110I)

Ref Sequence

ENSEMBL: ENSMUSP00000144716 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000079810] [ENSMUST00000204979]

AlphaFold

Q7TRH8

Predicted Effect

probably damaging
Transcript: ENSMUST00000079810
AA Change: F110I

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000078739
Gene: ENSMUSG00000095002
AA Change: F110I

Domain	Start	End	E-Value	Type
Pfam:7tm_4	28	309	5e-52	PFAM
Pfam:7tm_1	39	288	2.1e-25	PFAM

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000203614

Predicted Effect

probably damaging
Transcript: ENSMUST00000204979
AA Change: F110I

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000144716
Gene: ENSMUSG00000095002
AA Change: F110I

Domain	Start	End	E-Value	Type
Pfam:7tm_4	28	309	5e-52	PFAM
Pfam:7tm_1	39	288	2.1e-25	PFAM

Coding Region Coverage

1x: 99.3%
3x: 98.7%
10x: 97.6%
20x: 96.1%

Validation Efficiency

MGI Phenotype

FUNCTION: Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]

Allele List at MGI

Other mutations in this stock

Total: 58 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
1700017B05Rik	T	A	9: 57,164,468 (GRCm39)	K635N	probably damaging	Het
Afg3l2	G	T	18: 67,554,329 (GRCm39)	L458M	probably damaging	Het
Ahnak	T	A	19: 8,992,705 (GRCm39)	M4663K	possibly damaging	Het
C4b	T	A	17: 34,960,212 (GRCm39)	T280S	probably benign	Het
Ccdc166	A	G	15: 75,852,869 (GRCm39)	V366A	probably benign	Het
Cdh23	A	G	10: 60,493,044 (GRCm39)		probably null	Het
Col7a1	A	G	9: 108,792,292 (GRCm39)	T1281A	unknown	Het
Cul9	TTCCTCCTCCTCCTCCTCCTCCTC	TTCCTCCTCCTCCTCCTCCTC	17: 46,811,775 (GRCm39)		probably benign	Het
Dbndd1	T	A	8: 124,233,484 (GRCm39)	D127V	probably damaging	Het
Ddx20	T	A	3: 105,590,825 (GRCm39)	E197V	probably damaging	Het
Dennd10	A	G	19: 60,814,324 (GRCm39)	M96V	probably benign	Het
Dnm3	A	G	1: 161,838,471 (GRCm39)	S826P	probably damaging	Het
Dnmt3a	T	C	12: 3,945,643 (GRCm39)	V24A	probably benign	Het
Dop1b	C	A	16: 93,567,392 (GRCm39)	A1273E	probably damaging	Het
Dsg1c	T	C	18: 20,416,703 (GRCm39)	V868A	possibly damaging	Het
Dtx4	A	G	19: 12,462,626 (GRCm39)		probably null	Het
Elovl4	A	G	9: 83,672,154 (GRCm39)	L55P	possibly damaging	Het
Fbxo40	T	A	16: 36,789,914 (GRCm39)	T399S	possibly damaging	Het
Fkbpl	G	A	17: 34,864,303 (GRCm39)	A24T	probably benign	Het
Gm14399	T	C	2: 174,973,371 (GRCm39)	E96G	probably damaging	Het
Gm14496	T	G	2: 181,642,147 (GRCm39)	V606G	probably benign	Het
Gpr156	A	G	16: 37,826,087 (GRCm39)	E768G	probably damaging	Het
Grm5	A	T	7: 87,723,704 (GRCm39)	T665S	probably damaging	Het
Hsdl2	A	G	4: 59,592,301 (GRCm39)		probably benign	Het
Htt	T	C	5: 35,064,928 (GRCm39)	V3047A	possibly damaging	Het
Igkv3-2	A	T	6: 70,676,011 (GRCm39)	T107S	probably benign	Het
Insyn2b	G	A	11: 34,352,788 (GRCm39)	E277K	probably damaging	Het
Itih2	T	A	2: 10,101,272 (GRCm39)	T899S	probably benign	Het
Lhcgr	T	A	17: 89,050,281 (GRCm39)	Y415F	probably damaging	Het
Ltbr	G	A	6: 125,289,757 (GRCm39)	R146W	probably damaging	Het
Med4	C	A	14: 73,747,553 (GRCm39)	S18*	probably null	Het
Nefl	T	C	14: 68,322,088 (GRCm39)	V226A	probably damaging	Het
Nploc4	T	A	11: 120,275,389 (GRCm39)	N516Y	probably damaging	Het
Or1o4	A	G	17: 37,590,501 (GRCm39)	V270A	probably benign	Het
Otx1	C	A	11: 21,947,037 (GRCm39)	A91S	probably damaging	Het
Pcdha3	T	G	18: 37,079,752 (GRCm39)	L165V	possibly damaging	Het
Pcdhb12	T	A	18: 37,570,099 (GRCm39)	V415D	probably damaging	Het
Pcdhga10	T	C	18: 37,880,503 (GRCm39)	I88T	probably damaging	Het
Pigyl	T	A	9: 22,069,292 (GRCm39)	M1K	probably null	Het
Prr27	T	C	5: 87,991,203 (GRCm39)	S272P	probably damaging	Het
Prss3	C	T	6: 41,350,780 (GRCm39)	D237N	probably benign	Het
Pstpip2	A	G	18: 77,958,078 (GRCm39)	D150G	probably damaging	Het
Robo4	T	A	9: 37,324,674 (GRCm39)	D909E	probably benign	Het
Serpinb3c	A	T	1: 107,204,661 (GRCm39)	Y28*	probably null	Het
Slc26a3	T	C	12: 31,500,980 (GRCm39)		probably null	Het
Slc6a1	A	T	6: 114,279,493 (GRCm39)	I91F	probably benign	Het
Smcr8	T	G	11: 60,669,118 (GRCm39)	Y89D	probably damaging	Het
Sspo	T	A	6: 48,443,247 (GRCm39)	M1898K	probably benign	Het
Tbl1xr1	T	A	3: 22,246,233 (GRCm39)	I251K	probably damaging	Het
Thbs4	C	T	13: 92,913,501 (GRCm39)	D140N	probably benign	Het
Tmbim6	G	A	15: 99,303,633 (GRCm39)	A108T	probably benign	Het
Trim10	T	G	17: 37,186,328 (GRCm39)	L301R	probably benign	Het
Trpm7	A	T	2: 126,671,161 (GRCm39)	I607N	possibly damaging	Het
Vmn2r9	T	A	5: 108,995,929 (GRCm39)	I240F	probably damaging	Het
Xpot	T	A	10: 121,436,765 (GRCm39)	I873F	possibly damaging	Het
Zfhx4	G	A	3: 5,464,267 (GRCm39)	S1475N	probably damaging	Het
Zfp712	A	G	13: 67,189,079 (GRCm39)	S483P	possibly damaging	Het
Zswim7	A	T	11: 62,158,373 (GRCm39)	H122Q	probably benign	Het

Other mutations in Or6c66

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01093:Or6c66	APN	10	129,461,432 (GRCm39)	missense	probably damaging	1.00
IGL02866:Or6c66	APN	10	129,461,575 (GRCm39)	nonsense	probably null
BB009:Or6c66	UTSW	10	129,461,094 (GRCm39)	missense	probably damaging	0.97
BB019:Or6c66	UTSW	10	129,461,094 (GRCm39)	missense	probably damaging	0.97
R0743:Or6c66	UTSW	10	129,461,712 (GRCm39)	missense	probably benign	0.03
R1163:Or6c66	UTSW	10	129,461,516 (GRCm39)	missense	possibly damaging	0.79
R1192:Or6c66	UTSW	10	129,461,906 (GRCm39)	missense	probably benign
R1863:Or6c66	UTSW	10	129,461,217 (GRCm39)	missense	probably damaging	0.96
R1867:Or6c66	UTSW	10	129,461,621 (GRCm39)	missense	probably damaging	0.99
R2508:Or6c66	UTSW	10	129,461,784 (GRCm39)	missense	probably benign
R4898:Or6c66	UTSW	10	129,461,468 (GRCm39)	missense	probably benign	0.12
R4910:Or6c66	UTSW	10	129,461,676 (GRCm39)	missense	probably damaging	0.98
R4988:Or6c66	UTSW	10	129,461,930 (GRCm39)	splice site	probably null
R5382:Or6c66	UTSW	10	129,461,876 (GRCm39)	missense	probably damaging	0.98
R5589:Or6c66	UTSW	10	129,461,319 (GRCm39)	missense	probably damaging	1.00
R5693:Or6c66	UTSW	10	129,461,396 (GRCm39)	missense	probably damaging	0.99
R5790:Or6c66	UTSW	10	129,461,757 (GRCm39)	missense	probably damaging	1.00
R6966:Or6c66	UTSW	10	129,461,633 (GRCm39)	missense	probably benign	0.17
R7041:Or6c66	UTSW	10	129,461,603 (GRCm39)	missense	probably damaging	1.00
R7082:Or6c66	UTSW	10	129,461,634 (GRCm39)	missense	probably benign	0.07
R7932:Or6c66	UTSW	10	129,461,094 (GRCm39)	missense	probably damaging	0.97
R7977:Or6c66	UTSW	10	129,461,838 (GRCm39)	missense	probably benign
R7987:Or6c66	UTSW	10	129,461,838 (GRCm39)	missense	probably benign
R8299:Or6c66	UTSW	10	129,461,829 (GRCm39)	missense	probably benign	0.00
R8487:Or6c66	UTSW	10	129,461,114 (GRCm39)	missense	possibly damaging	0.50
R9110:Or6c66	UTSW	10	129,461,820 (GRCm39)	missense	possibly damaging	0.88

Predicted Primers

PCR Primer
(F):5'- TCACAGAAATCCAGTTGAAGGCC -3'
(R):5'- TGAGCATTACTGGAAATCTGACC -3'

Sequencing Primer
(F):5'- TCCAGTTGAAGGCCCATGATC -3'
(R):5'- CTGGAAATCTGACCATTATTACCC -3'

Posted On

2016-08-04