Incidental Mutation 'R5361:Nefl'
ID 422825
Institutional Source Beutler Lab
Gene Symbol Nefl
Ensembl Gene ENSMUSG00000022055
Gene Name neurofilament, light polypeptide
Synonyms NF68, NF-L, Nfl, CMT2E
MMRRC Submission 042940-MU
Accession Numbers
Essential gene? Non essential (E-score: 0.000) question?
Stock # R5361 (G1)
Quality Score 225
Status Not validated
Chromosome 14
Chromosomal Location 68321312-68326544 bp(+) (GRCm39)
Type of Mutation missense
DNA Base Change (assembly) T to C at 68322088 bp (GRCm39)
Zygosity Heterozygous
Amino Acid Change Valine to Alanine at position 226 (V226A)
Ref Sequence ENSEMBL: ENSMUSP00000022639 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000022639] [ENSMUST00000111089]
AlphaFold P08551
Predicted Effect probably damaging
Transcript: ENSMUST00000022639
AA Change: V226A

PolyPhen 2 Score 0.989 (Sensitivity: 0.72; Specificity: 0.97)
SMART Domains Protein: ENSMUSP00000022639
Gene: ENSMUSG00000022055
AA Change: V226A

DomainStartEndE-ValueType
Pfam:Filament_head 9 88 7e-14 PFAM
Filament 89 400 6.93e-139 SMART
low complexity region 448 470 N/A INTRINSIC
coiled coil region 473 512 N/A INTRINSIC
Predicted Effect probably benign
Transcript: ENSMUST00000111089
SMART Domains Protein: ENSMUSP00000106718
Gene: ENSMUSG00000022054

DomainStartEndE-ValueType
Pfam:Filament_head 9 97 1.6e-16 PFAM
Pfam:Filament 98 403 1.1e-104 PFAM
Coding Region Coverage
  • 1x: 99.3%
  • 3x: 98.7%
  • 10x: 97.6%
  • 20x: 96.1%
Validation Efficiency
MGI Phenotype FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] Neurofilaments are type IV intermediate filament heteropolymers composed of light, medium, and heavy chains. Neurofilaments comprise the axoskeleton and they functionally maintain the neuronal caliber. They may also play a role in intracellular transport to axons and dendrites. This gene encodes the light chain neurofilament protein. Mutations in this gene cause Charcot-Marie-Tooth disease types 1F (CMT1F) and 2E (CMT2E), disorders of the peripheral nervous system that are characterized by distinct neuropathies. A pseudogene has been identified on chromosome Y. [provided by RefSeq, Oct 2008]
PHENOTYPE: Mice homozygous for disruptions of this gene lack neurofilaments in their axons and have motor axons that are reduced in both size and number. [provided by MGI curators]
Allele List at MGI
Other mutations in this stock
Total: 58 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
1700017B05Rik T A 9: 57,164,468 (GRCm39) K635N probably damaging Het
Afg3l2 G T 18: 67,554,329 (GRCm39) L458M probably damaging Het
Ahnak T A 19: 8,992,705 (GRCm39) M4663K possibly damaging Het
C4b T A 17: 34,960,212 (GRCm39) T280S probably benign Het
Ccdc166 A G 15: 75,852,869 (GRCm39) V366A probably benign Het
Cdh23 A G 10: 60,493,044 (GRCm39) probably null Het
Col7a1 A G 9: 108,792,292 (GRCm39) T1281A unknown Het
Cul9 TTCCTCCTCCTCCTCCTCCTCCTC TTCCTCCTCCTCCTCCTCCTC 17: 46,811,775 (GRCm39) probably benign Het
Dbndd1 T A 8: 124,233,484 (GRCm39) D127V probably damaging Het
Ddx20 T A 3: 105,590,825 (GRCm39) E197V probably damaging Het
Dennd10 A G 19: 60,814,324 (GRCm39) M96V probably benign Het
Dnm3 A G 1: 161,838,471 (GRCm39) S826P probably damaging Het
Dnmt3a T C 12: 3,945,643 (GRCm39) V24A probably benign Het
Dop1b C A 16: 93,567,392 (GRCm39) A1273E probably damaging Het
Dsg1c T C 18: 20,416,703 (GRCm39) V868A possibly damaging Het
Dtx4 A G 19: 12,462,626 (GRCm39) probably null Het
Elovl4 A G 9: 83,672,154 (GRCm39) L55P possibly damaging Het
Fbxo40 T A 16: 36,789,914 (GRCm39) T399S possibly damaging Het
Fkbpl G A 17: 34,864,303 (GRCm39) A24T probably benign Het
Gm14399 T C 2: 174,973,371 (GRCm39) E96G probably damaging Het
Gm14496 T G 2: 181,642,147 (GRCm39) V606G probably benign Het
Gpr156 A G 16: 37,826,087 (GRCm39) E768G probably damaging Het
Grm5 A T 7: 87,723,704 (GRCm39) T665S probably damaging Het
Hsdl2 A G 4: 59,592,301 (GRCm39) probably benign Het
Htt T C 5: 35,064,928 (GRCm39) V3047A possibly damaging Het
Igkv3-2 A T 6: 70,676,011 (GRCm39) T107S probably benign Het
Insyn2b G A 11: 34,352,788 (GRCm39) E277K probably damaging Het
Itih2 T A 2: 10,101,272 (GRCm39) T899S probably benign Het
Lhcgr T A 17: 89,050,281 (GRCm39) Y415F probably damaging Het
Ltbr G A 6: 125,289,757 (GRCm39) R146W probably damaging Het
Med4 C A 14: 73,747,553 (GRCm39) S18* probably null Het
Nploc4 T A 11: 120,275,389 (GRCm39) N516Y probably damaging Het
Or1o4 A G 17: 37,590,501 (GRCm39) V270A probably benign Het
Or6c66 A T 10: 129,461,601 (GRCm39) F110I probably damaging Het
Otx1 C A 11: 21,947,037 (GRCm39) A91S probably damaging Het
Pcdha3 T G 18: 37,079,752 (GRCm39) L165V possibly damaging Het
Pcdhb12 T A 18: 37,570,099 (GRCm39) V415D probably damaging Het
Pcdhga10 T C 18: 37,880,503 (GRCm39) I88T probably damaging Het
Pigyl T A 9: 22,069,292 (GRCm39) M1K probably null Het
Prr27 T C 5: 87,991,203 (GRCm39) S272P probably damaging Het
Prss3 C T 6: 41,350,780 (GRCm39) D237N probably benign Het
Pstpip2 A G 18: 77,958,078 (GRCm39) D150G probably damaging Het
Robo4 T A 9: 37,324,674 (GRCm39) D909E probably benign Het
Serpinb3c A T 1: 107,204,661 (GRCm39) Y28* probably null Het
Slc26a3 T C 12: 31,500,980 (GRCm39) probably null Het
Slc6a1 A T 6: 114,279,493 (GRCm39) I91F probably benign Het
Smcr8 T G 11: 60,669,118 (GRCm39) Y89D probably damaging Het
Sspo T A 6: 48,443,247 (GRCm39) M1898K probably benign Het
Tbl1xr1 T A 3: 22,246,233 (GRCm39) I251K probably damaging Het
Thbs4 C T 13: 92,913,501 (GRCm39) D140N probably benign Het
Tmbim6 G A 15: 99,303,633 (GRCm39) A108T probably benign Het
Trim10 T G 17: 37,186,328 (GRCm39) L301R probably benign Het
Trpm7 A T 2: 126,671,161 (GRCm39) I607N possibly damaging Het
Vmn2r9 T A 5: 108,995,929 (GRCm39) I240F probably damaging Het
Xpot T A 10: 121,436,765 (GRCm39) I873F possibly damaging Het
Zfhx4 G A 3: 5,464,267 (GRCm39) S1475N probably damaging Het
Zfp712 A G 13: 67,189,079 (GRCm39) S483P possibly damaging Het
Zswim7 A T 11: 62,158,373 (GRCm39) H122Q probably benign Het
Other mutations in Nefl
AlleleSourceChrCoordTypePredicted EffectPPH Score
IGL01339:Nefl APN 14 68,323,931 (GRCm39) intron probably benign
IGL01755:Nefl APN 14 68,323,526 (GRCm39) missense probably damaging 1.00
IGL02825:Nefl APN 14 68,321,795 (GRCm39) missense possibly damaging 0.96
IGL03297:Nefl APN 14 68,321,673 (GRCm39) missense possibly damaging 0.55
PIT4418001:Nefl UTSW 14 68,323,979 (GRCm39) missense probably damaging 0.99
R0503:Nefl UTSW 14 68,321,432 (GRCm39) missense probably benign 0.08
R1837:Nefl UTSW 14 68,324,075 (GRCm39) missense probably damaging 1.00
R1970:Nefl UTSW 14 68,324,121 (GRCm39) missense probably benign 0.20
R4812:Nefl UTSW 14 68,321,734 (GRCm39) missense probably damaging 1.00
R4972:Nefl UTSW 14 68,324,212 (GRCm39) intron probably benign
R6357:Nefl UTSW 14 68,321,767 (GRCm39) missense probably damaging 1.00
R6499:Nefl UTSW 14 68,322,034 (GRCm39) missense probably damaging 1.00
R7571:Nefl UTSW 14 68,322,123 (GRCm39) missense probably benign 0.00
R8086:Nefl UTSW 14 68,323,480 (GRCm39) missense probably damaging 0.98
R9325:Nefl UTSW 14 68,322,460 (GRCm39) critical splice donor site probably null
R9582:Nefl UTSW 14 68,324,849 (GRCm39) missense unknown
Predicted Primers PCR Primer
(F):5'- AAACACTCTGAGCCTTCCCG -3'
(R):5'- GCTCTTGAACCACTCTTCGG -3'

Sequencing Primer
(F):5'- CTGTACGAGCAGGAGATCCG -3'
(R):5'- GAACCACTCTTCGGCGTTCTG -3'
Posted On 2016-08-04