Incidental Mutation 'R5361:Trim10'
ID422835
Institutional Source Beutler Lab
Gene Symbol Trim10
Ensembl Gene ENSMUSG00000073400
Gene Nametripartite motif-containing 10
SynonymsRnf9, Herf1
MMRRC Submission 042940-MU
Accession Numbers
Is this an essential gene? Probably non essential (E-score: 0.115) question?
Stock #R5361 (G1)
Quality Score225
Status Not validated
Chromosome17
Chromosomal Location36869574-36877833 bp(+) (GRCm38)
Type of Mutationmissense
DNA Base Change (assembly) T to G at 36875436 bp
ZygosityHeterozygous
Amino Acid Change Leucine to Arginine at position 301 (L301R)
Ref Sequence ENSEMBL: ENSMUSP00000057928 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000060524]
Predicted Effect probably benign
Transcript: ENSMUST00000060524
AA Change: L301R

PolyPhen 2 Score 0.026 (Sensitivity: 0.95; Specificity: 0.81)
SMART Domains Protein: ENSMUSP00000057928
Gene: ENSMUSG00000073400
AA Change: L301R

DomainStartEndE-ValueType
RING 16 60 1.2e-7 SMART
BBOX 94 135 5.38e-10 SMART
coiled coil region 152 175 N/A INTRINSIC
low complexity region 187 207 N/A INTRINSIC
PRY 309 361 1.04e-25 SMART
SPRY 362 485 1.51e-23 SMART
Coding Region Coverage
  • 1x: 99.3%
  • 3x: 98.7%
  • 10x: 97.6%
  • 20x: 96.1%
Validation Efficiency
MGI Phenotype FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. This protein localizes to cytoplasmic bodies. Studies in mice suggest that this protein plays a role in terminal differentiation of erythroid cells. Alternate splicing of this gene generates two transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]
Allele List at MGI
Other mutations in this stock
Total: 58 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
1700017B05Rik T A 9: 57,257,185 K635N probably damaging Het
Afg3l2 G T 18: 67,421,259 L458M probably damaging Het
Ahnak T A 19: 9,015,341 M4663K possibly damaging Het
C4b T A 17: 34,741,238 T280S probably benign Het
Ccdc166 A G 15: 75,981,020 V366A probably benign Het
Cdh23 A G 10: 60,657,265 probably null Het
Col7a1 A G 9: 108,963,224 T1281A unknown Het
Cul9 TTCCTCCTCCTCCTCCTCCTCCTC TTCCTCCTCCTCCTCCTCCTC 17: 46,500,849 probably benign Het
Dbndd1 T A 8: 123,506,745 D127V probably damaging Het
Ddx20 T A 3: 105,683,509 E197V probably damaging Het
Dnm3 A G 1: 162,010,902 S826P probably damaging Het
Dnmt3a T C 12: 3,895,643 V24A probably benign Het
Dopey2 C A 16: 93,770,504 A1273E probably damaging Het
Dsg1c T C 18: 20,283,646 V868A possibly damaging Het
Dtx4 A G 19: 12,485,262 probably null Het
Elovl4 A G 9: 83,790,101 L55P possibly damaging Het
Fam196b G A 11: 34,402,788 E277K probably damaging Het
Fam45a A G 19: 60,825,886 M96V probably benign Het
Fbxo40 T A 16: 36,969,552 T399S possibly damaging Het
Fkbpl G A 17: 34,645,329 A24T probably benign Het
Gm14399 T C 2: 175,131,578 E96G probably damaging Het
Gm14496 T G 2: 182,000,354 V606G probably benign Het
Gpr156 A G 16: 38,005,725 E768G probably damaging Het
Grm5 A T 7: 88,074,496 T665S probably damaging Het
Hsdl2 A G 4: 59,592,301 probably benign Het
Htt T C 5: 34,907,584 V3047A possibly damaging Het
Igkv3-2 A T 6: 70,699,027 T107S probably benign Het
Itih2 T A 2: 10,096,461 T899S probably benign Het
Lhcgr T A 17: 88,742,853 Y415F probably damaging Het
Ltbr G A 6: 125,312,794 R146W probably damaging Het
Med4 C A 14: 73,510,113 S18* probably null Het
Nefl T C 14: 68,084,639 V226A probably damaging Het
Nploc4 T A 11: 120,384,563 N516Y probably damaging Het
Olfr798 A T 10: 129,625,732 F110I probably damaging Het
Olfr99 A G 17: 37,279,610 V270A probably benign Het
Otx1 C A 11: 21,997,037 A91S probably damaging Het
Pcdha3 T G 18: 36,946,699 L165V possibly damaging Het
Pcdhb12 T A 18: 37,437,046 V415D probably damaging Het
Pcdhga10 T C 18: 37,747,450 I88T probably damaging Het
Pigyl T A 9: 22,157,996 M1K probably null Het
Prr27 T C 5: 87,843,344 S272P probably damaging Het
Prss3 C T 6: 41,373,846 D237N probably benign Het
Pstpip2 A G 18: 77,870,378 D150G probably damaging Het
Robo4 T A 9: 37,413,378 D909E probably benign Het
Serpinb3c A T 1: 107,276,931 Y28* probably null Het
Slc26a3 T C 12: 31,450,981 probably null Het
Slc6a1 A T 6: 114,302,532 I91F probably benign Het
Smcr8 T G 11: 60,778,292 Y89D probably damaging Het
Sspo T A 6: 48,466,313 M1898K probably benign Het
Tbl1xr1 T A 3: 22,192,069 I251K probably damaging Het
Thbs4 C T 13: 92,776,993 D140N probably benign Het
Tmbim6 G A 15: 99,405,752 A108T probably benign Het
Trpm7 A T 2: 126,829,241 I607N possibly damaging Het
Vmn2r9 T A 5: 108,848,063 I240F probably damaging Het
Xpot T A 10: 121,600,860 I873F possibly damaging Het
Zfhx4 G A 3: 5,399,207 S1475N probably damaging Het
Zfp712 A G 13: 67,041,015 S483P possibly damaging Het
Zswim7 A T 11: 62,267,547 H122Q probably benign Het
Other mutations in Trim10
AlleleSourceChrCoordTypePredicted EffectPPH Score
IGL00493:Trim10 APN 17 36877248 missense probably benign 0.03
IGL00501:Trim10 APN 17 36877047 missense probably benign 0.08
IGL00846:Trim10 APN 17 36871692 missense probably damaging 1.00
IGL01536:Trim10 APN 17 36877288 unclassified probably null
IGL02814:Trim10 APN 17 36877336 nonsense probably null
IGL03135:Trim10 APN 17 36874221 missense possibly damaging 0.78
IGL03144:Trim10 APN 17 36876848 missense probably damaging 1.00
IGL03298:Trim10 APN 17 36877025 missense possibly damaging 0.87
PIT4378001:Trim10 UTSW 17 36877128 missense probably damaging 0.98
R0102:Trim10 UTSW 17 36870182 missense probably damaging 1.00
R0102:Trim10 UTSW 17 36870182 missense probably damaging 1.00
R0834:Trim10 UTSW 17 36872391 missense probably benign 0.00
R1517:Trim10 UTSW 17 36872454 missense probably damaging 1.00
R1691:Trim10 UTSW 17 36876899 missense probably damaging 1.00
R1696:Trim10 UTSW 17 36877181 nonsense probably null
R2149:Trim10 UTSW 17 36877014 missense probably benign 0.18
R3153:Trim10 UTSW 17 36871688 missense probably damaging 1.00
R3154:Trim10 UTSW 17 36871688 missense probably damaging 1.00
R5156:Trim10 UTSW 17 36877056 missense probably damaging 0.99
R5327:Trim10 UTSW 17 36870189 missense probably damaging 1.00
R5758:Trim10 UTSW 17 36877152 missense possibly damaging 0.80
R5764:Trim10 UTSW 17 36870181 missense probably damaging 0.97
R6032:Trim10 UTSW 17 36871714 missense possibly damaging 0.87
R6032:Trim10 UTSW 17 36871714 missense possibly damaging 0.87
R6179:Trim10 UTSW 17 36877031 missense probably damaging 1.00
R6709:Trim10 UTSW 17 36872370 missense probably damaging 0.99
R7172:Trim10 UTSW 17 36870063 missense possibly damaging 0.78
R7197:Trim10 UTSW 17 36876954 missense probably damaging 1.00
R7390:Trim10 UTSW 17 36869881 start codon destroyed probably null 0.98
R7391:Trim10 UTSW 17 36869881 start codon destroyed probably null 0.98
Predicted Primers PCR Primer
(F):5'- AGGCCAGGAGCATCAGTAAC -3'
(R):5'- AACTGTCATACATGGCCTCCTC -3'

Sequencing Primer
(F):5'- ACCCATGTGAGATACCTGCATATCTG -3'
(R):5'- GGGCAGAATCTTACCATATCACTAC -3'
Posted On2016-08-04