Incidental Mutation 'R5336:Zfp874a'
| ID |
423536 |
| Institutional Source |
Beutler Lab
|
| Gene Symbol |
Zfp874a
|
| Ensembl Gene |
ENSMUSG00000069206 |
| Gene Name |
zinc finger protein 874a |
| Synonyms |
C330011K17Rik, Zfp874, Rslcan15 |
| MMRRC Submission |
042844-MU
|
| Accession Numbers |
|
| Essential gene? |
Probably non essential
(E-score: 0.077)
|
| Stock # |
R5336 (G1)
|
| Quality Score |
225 |
|
Status
|
Not validated
|
| Chromosome |
13 |
| Chromosomal Location |
67588550-67599743 bp(-) (GRCm39) |
| Type of Mutation |
missense |
| DNA Base Change (assembly) |
T to C
at 67590799 bp (GRCm39)
|
| Zygosity |
Heterozygous |
| Amino Acid Change |
Histidine to Arginine
at position 295
(H295R)
|
| Ref Sequence |
ENSEMBL: ENSMUSP00000074735
(fasta)
|
| Gene Model |
predicted gene model for transcript(s):
[ENSMUST00000057241]
[ENSMUST00000075255]
[ENSMUST00000180580]
[ENSMUST00000223682]
[ENSMUST00000225479]
|
| AlphaFold |
Q8BX23 |
| Predicted Effect |
probably benign
Transcript: ENSMUST00000057241
|
| SMART Domains |
Protein: ENSMUSP00000077307
Gene: ENSMUSG00000069206
| Domain | Start | End | E-Value | Type |
|---|
|
KRAB
|
5 |
65 |
1.73e-27 |
SMART |
|
| Predicted Effect |
probably damaging
Transcript: ENSMUST00000075255
AA Change: H295R
PolyPhen 2
Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)
|
| SMART Domains |
Protein: ENSMUSP00000074735
Gene: ENSMUSG00000069206
AA Change: H295R
| Domain | Start | End | E-Value | Type |
|---|
|
KRAB
|
5 |
65 |
1.73e-27 |
SMART |
|
ZnF_C2H2
|
137 |
159 |
9.58e-3 |
SMART |
|
ZnF_C2H2
|
165 |
187 |
5.99e-4 |
SMART |
|
ZnF_C2H2
|
193 |
215 |
7.26e-3 |
SMART |
|
ZnF_C2H2
|
221 |
243 |
2.4e-3 |
SMART |
|
ZnF_C2H2
|
249 |
269 |
1.88e2 |
SMART |
|
ZnF_C2H2
|
277 |
299 |
5.21e-4 |
SMART |
|
ZnF_C2H2
|
305 |
327 |
2.12e-4 |
SMART |
|
ZnF_C2H2
|
333 |
355 |
2.12e-4 |
SMART |
|
ZnF_C2H2
|
361 |
383 |
8.47e-4 |
SMART |
|
ZnF_C2H2
|
389 |
411 |
1.79e-2 |
SMART |
|
ZnF_C2H2
|
417 |
438 |
2.86e-1 |
SMART |
|
| Predicted Effect |
probably benign
Transcript: ENSMUST00000180580
|
| SMART Domains |
Protein: ENSMUSP00000137953
Gene: ENSMUSG00000069206
| Domain | Start | End | E-Value | Type |
|---|
|
KRAB
|
5 |
65 |
1.73e-27 |
SMART |
|
| Predicted Effect |
probably benign
Transcript: ENSMUST00000223682
|
| Predicted Effect |
noncoding transcript
Transcript: ENSMUST00000224239
|
| Predicted Effect |
probably benign
Transcript: ENSMUST00000225479
|
| Predicted Effect |
probably benign
Transcript: ENSMUST00000225778
|
| Coding Region Coverage |
- 1x: 99.3%
- 3x: 98.8%
- 10x: 97.5%
- 20x: 96.0%
|
| Validation Efficiency |
|
| Allele List at MGI |
|
| Other mutations in this stock |
Total: 34 list
| Gene | Ref | Var | Chr/Loc | Mutation | Predicted Effect | Zygosity |
|---|
| 9330159F19Rik |
A |
T |
10: 29,100,495 (GRCm39) |
E289D |
probably damaging |
Het |
| Acyp1 |
A |
T |
12: 85,326,785 (GRCm39) |
F80I |
probably damaging |
Het |
| Anapc5 |
T |
C |
5: 122,945,400 (GRCm39) |
N226S |
probably damaging |
Het |
| Ankhd1 |
T |
A |
18: 36,779,769 (GRCm39) |
S1704T |
probably damaging |
Het |
| Btnl5-ps |
T |
C |
17: 34,711,371 (GRCm39) |
|
noncoding transcript |
Het |
| Cacna1h |
T |
C |
17: 25,611,205 (GRCm39) |
H531R |
probably damaging |
Het |
| Chpf |
C |
A |
1: 75,452,351 (GRCm39) |
A368S |
possibly damaging |
Het |
| Cntn4 |
T |
C |
6: 106,639,595 (GRCm39) |
V575A |
possibly damaging |
Het |
| Col5a3 |
A |
T |
9: 20,710,597 (GRCm39) |
F551I |
unknown |
Het |
| Ctnnd1 |
C |
T |
2: 84,447,133 (GRCm39) |
V371M |
probably damaging |
Het |
| Dcxr |
A |
G |
11: 120,618,002 (GRCm39) |
|
probably null |
Het |
| Ddost |
T |
C |
4: 138,036,741 (GRCm39) |
Y147H |
possibly damaging |
Het |
| Eif2ak2 |
A |
G |
17: 79,181,472 (GRCm39) |
F92L |
probably benign |
Het |
| Gad1-ps |
G |
A |
10: 99,281,009 (GRCm39) |
|
noncoding transcript |
Het |
| H2-T23 |
T |
A |
17: 36,342,550 (GRCm39) |
N196I |
possibly damaging |
Het |
| Ighv9-2 |
A |
C |
12: 114,072,905 (GRCm39) |
F23V |
probably benign |
Het |
| Igsf10 |
T |
C |
3: 59,227,553 (GRCm39) |
E2040G |
probably damaging |
Het |
| Mtpn |
C |
T |
6: 35,489,225 (GRCm39) |
D100N |
probably benign |
Het |
| Myh4 |
A |
G |
11: 67,150,017 (GRCm39) |
|
probably null |
Het |
| Nlrp2 |
T |
C |
7: 5,331,118 (GRCm39) |
D426G |
probably benign |
Het |
| Or1a1 |
T |
C |
11: 74,086,859 (GRCm39) |
F177L |
probably damaging |
Het |
| Otof |
T |
C |
5: 30,534,064 (GRCm39) |
D1415G |
probably benign |
Het |
| Ppig |
T |
C |
2: 69,580,568 (GRCm39) |
S701P |
unknown |
Het |
| Ptprf |
A |
G |
4: 118,092,831 (GRCm39) |
I528T |
probably damaging |
Het |
| Scara3 |
A |
G |
14: 66,168,487 (GRCm39) |
Y377H |
probably damaging |
Het |
| Slf1 |
A |
T |
13: 77,254,129 (GRCm39) |
*176R |
probably null |
Het |
| Sntb1 |
C |
G |
15: 55,506,191 (GRCm39) |
G461R |
probably damaging |
Het |
| Tarm1 |
T |
C |
7: 3,546,084 (GRCm39) |
Y61C |
probably damaging |
Het |
| Tle4 |
A |
G |
19: 14,432,103 (GRCm39) |
|
probably null |
Het |
| Tlr1 |
A |
T |
5: 65,083,145 (GRCm39) |
N477K |
probably damaging |
Het |
| Tns2 |
C |
T |
15: 102,019,664 (GRCm39) |
T518M |
probably damaging |
Het |
| Trim50 |
C |
T |
5: 135,396,330 (GRCm39) |
T426M |
probably damaging |
Het |
| Vmn2r85 |
T |
C |
10: 130,258,574 (GRCm39) |
I494V |
possibly damaging |
Het |
| Wdr17 |
T |
A |
8: 55,085,353 (GRCm39) |
I1257F |
probably damaging |
Het |
|
| Other mutations in Zfp874a |
| Allele | Source | Chr | Coord | Type | Predicted Effect | PPH Score |
|---|
|
IGL02490:Zfp874a
|
APN |
13 |
67,590,819 (GRCm39) |
nonsense |
probably null |
|
|
R1052:Zfp874a
|
UTSW |
13 |
67,590,539 (GRCm39) |
missense |
possibly damaging |
0.89 |
|
R1991:Zfp874a
|
UTSW |
13 |
67,590,623 (GRCm39) |
missense |
probably benign |
0.03 |
|
R2085:Zfp874a
|
UTSW |
13 |
67,590,967 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R2103:Zfp874a
|
UTSW |
13 |
67,590,623 (GRCm39) |
missense |
probably benign |
0.03 |
|
R5129:Zfp874a
|
UTSW |
13 |
67,591,100 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R5423:Zfp874a
|
UTSW |
13 |
67,590,473 (GRCm39) |
missense |
possibly damaging |
0.75 |
|
R5713:Zfp874a
|
UTSW |
13 |
67,597,476 (GRCm39) |
missense |
probably benign |
0.16 |
|
R5855:Zfp874a
|
UTSW |
13 |
67,590,812 (GRCm39) |
missense |
probably benign |
0.03 |
|
R6496:Zfp874a
|
UTSW |
13 |
67,590,694 (GRCm39) |
missense |
possibly damaging |
0.62 |
|
R7046:Zfp874a
|
UTSW |
13 |
67,590,418 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R7065:Zfp874a
|
UTSW |
13 |
67,590,401 (GRCm39) |
missense |
probably damaging |
0.97 |
|
R7233:Zfp874a
|
UTSW |
13 |
67,590,776 (GRCm39) |
missense |
possibly damaging |
0.83 |
|
R7271:Zfp874a
|
UTSW |
13 |
67,591,415 (GRCm39) |
missense |
probably benign |
0.00 |
|
R7465:Zfp874a
|
UTSW |
13 |
67,590,376 (GRCm39) |
missense |
probably damaging |
0.99 |
|
R7468:Zfp874a
|
UTSW |
13 |
67,573,723 (GRCm39) |
splice site |
probably null |
|
|
R8108:Zfp874a
|
UTSW |
13 |
67,591,353 (GRCm39) |
nonsense |
probably null |
|
|
R8109:Zfp874a
|
UTSW |
13 |
67,597,563 (GRCm39) |
missense |
possibly damaging |
0.46 |
|
R8843:Zfp874a
|
UTSW |
13 |
67,590,764 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R8859:Zfp874a
|
UTSW |
13 |
67,590,647 (GRCm39) |
missense |
probably benign |
0.17 |
|
R9137:Zfp874a
|
UTSW |
13 |
67,590,841 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R9673:Zfp874a
|
UTSW |
13 |
67,591,156 (GRCm39) |
missense |
probably benign |
0.03 |
|
X0063:Zfp874a
|
UTSW |
13 |
67,591,106 (GRCm39) |
missense |
probably damaging |
1.00 |
|
Z1177:Zfp874a
|
UTSW |
13 |
67,590,781 (GRCm39) |
missense |
probably damaging |
1.00 |
|
| Predicted Primers |
PCR Primer
(F):5'- GATTTGATGTCTTTTCAGGGATGTAA -3'
(R):5'- CTCTTAGTAGTCGCTCATCACTT -3'
Sequencing Primer
(F):5'- GCATTCTACACACTCATGGGG -3'
(R):5'- TACTATCCTGCAATGCTGAAGC -3'
|
| Posted On |
2016-08-04 |
Incidental Mutation