Mutagenetix > Incidental Mutation

Incidental Mutation 'R5385:Nfkb1'

425057

Institutional Source

Beutler Lab

Gene Symbol

Nfkb1

Ensembl Gene

ENSMUSG00000028163

Gene Name

nuclear factor of kappa light polypeptide gene enhancer in B cells 1, p105

Synonyms

p50, p50/p105, NF-kappaB, nuclear factor kappaB p50, NF kappaB1, p50 subunit of NF kappaB, NF-kappaB p50

Accession Numbers

Essential gene?

Non essential (E-score: 0.000) question?

Stock #

R5385 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

135290416-135397308 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to G at 135318303 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Valine to Alanine at position 310 (V310A)

Ref Sequence

ENSEMBL: ENSMUSP00000128345 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000029812] [ENSMUST00000164430] [ENSMUST00000196469]

AlphaFold

P25799

PDB Structure

STRUCTURE OF NF-KB P50 HOMODIMER BOUND TO A KB SITE [X-RAY DIFFRACTION]
IKAPPABALPHA/NF-KAPPAB COMPLEX [X-RAY DIFFRACTION]
Crystal structure of a NF-kB heterodimer bound to an IFNb-kB [X-RAY DIFFRACTION]
Crystal structure of a NF-kB heterodimer bound to the Ig/HIV-kB siti [X-RAY DIFFRACTION]
The kB DNA sequence from the HLV-LTR functions as an allosteric regulator of HIV transcription [X-RAY DIFFRACTION]
STRUCTURE OF THE NUCLEAR FACTOR KAPPA-B (NF-KB) P50 HOMODIMER [X-RAY DIFFRACTION]
CRYSTAL STRUCTURE OF NF-kB(p50)2 COMPLEXED TO A HIGH-AFFINITY RNA APTAMER [X-RAY DIFFRACTION]
Crystal stucture of WLAC mutant of dimerisation domain of NF-kB p50 transcription factor [X-RAY DIFFRACTION]
Crystal stucture of MLAV mutant of dimerisation domain of NF-kB p50 transcription factor [X-RAY DIFFRACTION]
Crystal stucture of ILAC mutant of dimerisation domain of NF-kB p50 transcription factor [X-RAY DIFFRACTION]
>> 7 additional structures at PDB <<

Predicted Effect

possibly damaging
Transcript: ENSMUST00000029812
AA Change: V310A

PolyPhen 2 Score 0.949 (Sensitivity: 0.79; Specificity: 0.95)

SMART Domains

Protein: ENSMUSP00000029812
Gene: ENSMUSG00000028163
AA Change: V310A

Domain	Start	End	E-Value	Type
Pfam:RHD	42	240	2.9e-75	PFAM
IPT	247	348	1.14e-22	SMART
low complexity region	368	414	N/A	INTRINSIC
ANK	538	568	2.27e1	SMART
ANK	577	606	1.11e-2	SMART
ANK	610	640	2.47e0	SMART
ANK	646	675	5.53e-3	SMART
ANK	680	710	1.9e-1	SMART
ANK	714	743	2.18e-1	SMART
DEATH	801	888	1.9e-19	SMART
low complexity region	890	902	N/A	INTRINSIC

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000129428

Predicted Effect

probably benign
Transcript: ENSMUST00000132668

SMART Domains

Protein: ENSMUSP00000114798
Gene: ENSMUSG00000028163

Domain	Start	End	E-Value	Type
low complexity region	8	54	N/A	INTRINSIC
Blast:IPT	55	156	4e-22	BLAST
ANK	178	208	2.27e1	SMART
ANK	217	246	1.11e-2	SMART
ANK	250	280	2.47e0	SMART
ANK	286	315	5.53e-3	SMART
ANK	320	350	1.9e-1	SMART
ANK	354	383	2.18e-1	SMART
Blast:DEATH	441	505	1e-34	BLAST
PDB:2DBF\|A	442	505	5e-32	PDB

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000138602

Predicted Effect

possibly damaging
Transcript: ENSMUST00000164430
AA Change: V310A

PolyPhen 2 Score 0.949 (Sensitivity: 0.79; Specificity: 0.95)

SMART Domains

Protein: ENSMUSP00000128345
Gene: ENSMUSG00000028163
AA Change: V310A

Domain	Start	End	E-Value	Type
Pfam:RHD_DNA_bind	42	240	2.9e-75	PFAM
IPT	247	348	1.14e-22	SMART
low complexity region	368	414	N/A	INTRINSIC
ANK	538	568	2.27e1	SMART
ANK	577	606	1.11e-2	SMART
ANK	610	640	2.47e0	SMART
ANK	646	675	5.53e-3	SMART
ANK	680	710	1.9e-1	SMART
ANK	714	743	2.18e-1	SMART
DEATH	801	888	1.9e-19	SMART
low complexity region	890	902	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000196469

SMART Domains

Protein: ENSMUSP00000143601
Gene: ENSMUSG00000028163

Domain	Start	End	E-Value	Type
Pfam:RHD_DNA_bind	42	90	2.5e-19	PFAM

Coding Region Coverage

1x: 99.3%
3x: 98.7%
10x: 97.4%
20x: 95.7%

Validation Efficiency

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes a 105 kD protein which can undergo cotranslational processing by the 26S proteasome to produce a 50 kD protein. The 105 kD protein is a Rel protein-specific transcription inhibitor and the 50 kD protein is a DNA binding subunit of the NF-kappa-B (NFKB) protein complex. NFKB is a transcription regulator that is activated by various intra- and extra-cellular stimuli such as cytokines, oxidant-free radicals, ultraviolet irradiation, and bacterial or viral products. Activated NFKB translocates into the nucleus and stimulates the expression of genes involved in a wide variety of biological functions. Inappropriate activation of NFKB has been associated with a number of inflammatory diseases while persistent inhibition of NFKB leads to inappropriate immune cell development or delayed cell growth. Alternative splicing results in multiple transcript variants encoding different isoforms, at least one of which is proteolytically processed. [provided by RefSeq, Feb 2016]
PHENOTYPE: Homozygous null mice have a decreased survivor rate, abnormal T cell development and decreased number of peripheral T cells, abnormal humoral responses with decreased immunoglobulin class switching, exhibit mild organ inflammation, and are susceptible toboth bacterial infections and hearing loss. [provided by MGI curators]

Allele List at MGI

All alleles(79) : Targeted(5) Gene trapped(74)

Other mutations in this stock

Total: 129 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Abhd17a	T	C	10: 80,421,446 (GRCm39)	T175A	probably benign	Het
Adam23	A	G	1: 63,590,970 (GRCm39)	D479G	possibly damaging	Het
Adgrl3	T	G	5: 81,874,648 (GRCm39)	Y1050D	probably damaging	Het
Ago4	A	G	4: 126,411,349 (GRCm39)	I103T	probably benign	Het
Ajuba	T	C	14: 54,807,855 (GRCm39)	Y459C	probably damaging	Het
Aldh7a1	T	A	18: 56,667,325 (GRCm39)	N316Y	possibly damaging	Het
Alyref2	A	G	1: 171,331,271 (GRCm39)	N16S	probably benign	Het
Ankhd1	A	G	18: 36,724,548 (GRCm39)	E402G	probably damaging	Het
Ankrd36	A	G	11: 5,639,340 (GRCm39)		probably benign	Het
Ap2b1	T	C	11: 83,233,427 (GRCm39)	V480A	probably damaging	Het
Arhgap30	A	G	1: 171,235,848 (GRCm39)	R741G	probably benign	Het
Canx	A	G	11: 50,192,639 (GRCm39)	L325P	probably damaging	Het
Ccpg1	G	A	9: 72,920,326 (GRCm39)	S647N	probably benign	Het
Cdc37l1	T	G	19: 28,989,343 (GRCm39)	S267A	possibly damaging	Het
Cert1	A	G	13: 96,765,575 (GRCm39)	T447A	possibly damaging	Het
Ces1f	A	T	8: 93,992,388 (GRCm39)	C354*	probably null	Het
Cpne1	A	T	2: 155,916,284 (GRCm39)	V350D	probably damaging	Het
Ctdsp2	A	G	10: 126,832,326 (GRCm39)	T262A	probably benign	Het
Cypt4	A	G	9: 24,536,596 (GRCm39)	K29E	possibly damaging	Het
Dlg2	T	C	7: 91,737,784 (GRCm39)	V422A	probably damaging	Het
Dmxl2	A	G	9: 54,286,041 (GRCm39)	S2715P	probably benign	Het
Dnah3	T	A	7: 119,524,126 (GRCm39)	K3953N	probably damaging	Het
Dnmt1	A	G	9: 20,829,776 (GRCm39)	V647A	probably damaging	Het
Duox2	A	G	2: 122,125,617 (GRCm39)	V330A	probably benign	Het
Dusp8	T	A	7: 141,643,730 (GRCm39)	Q61L	possibly damaging	Het
Dync2h1	T	C	9: 7,016,791 (GRCm39)	D3573G	probably damaging	Het
Ears2	G	C	7: 121,643,600 (GRCm39)	T426S	probably benign	Het
Ext1	C	A	15: 52,939,213 (GRCm39)	W612L	probably damaging	Het
Fam91a1	G	A	15: 58,320,243 (GRCm39)	S645N	probably benign	Het
Fat3	A	T	9: 15,833,971 (GRCm39)	L4207Q	possibly damaging	Het
Fbxo38	A	G	18: 62,674,042 (GRCm39)	M13T	probably benign	Het
Fbxo48	G	T	11: 16,904,329 (GRCm39)	L160F	possibly damaging	Het
Fer1l4	G	A	2: 155,879,286 (GRCm39)	Q906*	probably null	Het
Fpr2	C	A	17: 18,113,309 (GRCm39)	H102N	probably benign	Het
Gm10134	T	A	2: 28,396,372 (GRCm39)		probably benign	Het
Gpr107	A	G	2: 31,104,263 (GRCm39)	T523A	probably benign	Het
Grin3a	G	A	4: 49,719,313 (GRCm39)	P811L	probably damaging	Het
Hivep1	A	T	13: 42,317,871 (GRCm39)		probably null	Het
Hmcn2	A	G	2: 31,350,333 (GRCm39)	T5077A	probably benign	Het
Hpse	C	T	5: 100,856,590 (GRCm39)	W136*	probably null	Het
Ifitm3	T	C	7: 140,590,554 (GRCm39)	N2S	probably benign	Het
Ifnar2	T	A	16: 91,201,086 (GRCm39)	D442E	possibly damaging	Het
Jade1	T	A	3: 41,546,137 (GRCm39)	I54N	probably damaging	Het
Jag1	A	T	2: 136,937,464 (GRCm39)	H303Q	possibly damaging	Het
Kcnh4	T	C	11: 100,643,076 (GRCm39)	D397G	probably damaging	Het
Kcnj1	A	G	9: 32,308,019 (GRCm39)	R148G	probably damaging	Het
Lct	T	G	1: 128,239,354 (GRCm39)	K277N	possibly damaging	Het
Loxl3	A	G	6: 83,027,593 (GRCm39)	M712V	probably damaging	Het
Ly75	A	G	2: 60,133,985 (GRCm39)	C1547R	probably damaging	Het
Marcks	A	G	10: 37,014,453 (GRCm39)	S27P	probably damaging	Het
Mef2c	A	G	13: 83,810,532 (GRCm39)	T347A	probably benign	Het
Mmp3	C	T	9: 7,451,759 (GRCm39)	R366*	probably null	Het
Mrgprx2	T	C	7: 48,132,753 (GRCm39)	T22A	probably benign	Het
Msc	C	A	1: 14,825,644 (GRCm39)	R110L	probably damaging	Het
Mtcl3	A	G	10: 29,072,766 (GRCm39)	D686G	probably benign	Het
Myh11	A	T	16: 14,025,872 (GRCm39)	V1366D	possibly damaging	Het
Ncf1	A	G	5: 134,250,659 (GRCm39)	L373P	probably damaging	Het
Neb	A	T	2: 52,079,873 (GRCm39)	I85N	probably damaging	Het
Nop2	T	C	6: 125,121,324 (GRCm39)	V702A	probably benign	Het
Nudt12	A	G	17: 59,310,434 (GRCm39)	W390R	probably damaging	Het
Or10ag53	C	T	2: 87,082,827 (GRCm39)	P182L	probably benign	Het
Or10v9	T	A	19: 11,832,541 (GRCm39)	I259F	probably damaging	Het
Or11g24	T	A	14: 50,662,846 (GRCm39)	V290E	possibly damaging	Het
Or1af1	G	T	2: 37,109,599 (GRCm39)	A33S	possibly damaging	Het
Or51e1	T	C	7: 102,358,553 (GRCm39)	L29P	probably damaging	Het
Or6c208	A	G	10: 129,223,633 (GRCm39)	I44V	probably benign	Het
Or6n2	A	T	1: 173,897,036 (GRCm39)	K57N	probably benign	Het
Or8a1b	A	T	9: 37,623,317 (GRCm39)	V86E	probably damaging	Het
Or8c11	A	T	9: 38,289,281 (GRCm39)	I29F	probably benign	Het
Or8g2b	A	G	9: 39,751,126 (GRCm39)	Y132C	possibly damaging	Het
Otub2	G	A	12: 103,359,055 (GRCm39)		probably benign	Het
Pck2	A	G	14: 55,782,688 (GRCm39)	E339G	probably damaging	Het
Pdcd7	G	A	9: 65,265,974 (GRCm39)	W477*	probably null	Het
Pdpk1	G	A	17: 24,317,114 (GRCm39)	Q250*	probably null	Het
Pigb	T	A	9: 72,946,827 (GRCm39)	H16L	probably benign	Het
Pitpnm1	T	C	19: 4,153,435 (GRCm39)	F197S	probably damaging	Het
Plekhh2	T	C	17: 84,864,894 (GRCm39)	V94A	probably benign	Het
Pnpla7	C	A	2: 24,931,031 (GRCm39)	P882Q	probably damaging	Het
Pold2	A	G	11: 5,826,760 (GRCm39)	L58P	probably damaging	Het
Pomt1	C	G	2: 32,134,311 (GRCm39)	Y277*	probably null	Het
Prex2	A	G	1: 11,210,204 (GRCm39)	D548G	probably damaging	Het
Prkra	T	G	2: 76,469,622 (GRCm39)	T146P	probably damaging	Het
Prss51	T	A	14: 64,334,543 (GRCm39)	V108E	probably damaging	Het
Rai2	A	G	X: 160,561,636 (GRCm39)	N363S	probably benign	Het
Ranbp17	A	T	11: 33,169,241 (GRCm39)	V991D	possibly damaging	Het
Riox2	T	A	16: 59,306,979 (GRCm39)	M290K	probably benign	Het
Rnpepl1	T	A	1: 92,844,914 (GRCm39)	L402Q	probably damaging	Het
S1pr2	T	C	9: 20,878,890 (GRCm39)	T313A	probably benign	Het
Sesn2	A	G	4: 132,226,575 (GRCm39)	I173T	probably damaging	Het
Setx	T	G	2: 29,024,045 (GRCm39)		probably null	Het
Sfi1	ACA	ACATCTTCCCAAAGCCAGTCA	11: 3,103,382 (GRCm39)		probably benign	Het
Sgsm3	T	C	15: 80,892,200 (GRCm39)	V256A	probably benign	Het
Shroom4	T	A	X: 6,497,523 (GRCm39)	C894*	probably null	Het
Slc13a5	T	A	11: 72,149,903 (GRCm39)	E159D	probably benign	Het
Slc16a7	A	G	10: 125,130,473 (GRCm39)	Y71H	possibly damaging	Het
Slc28a2b	C	A	2: 122,353,259 (GRCm39)	L480I	probably benign	Het
Slc4a7	T	C	14: 14,773,345 (GRCm38)	F772L	possibly damaging	Het
Snapc4	A	G	2: 26,264,515 (GRCm39)	S275P	probably benign	Het
Sorl1	T	C	9: 41,968,580 (GRCm39)	T558A	possibly damaging	Het
Sspo	C	T	6: 48,439,187 (GRCm39)	P1612S	probably benign	Het
Stard9	A	G	2: 120,531,111 (GRCm39)	E2456G	probably damaging	Het
Stx1b	A	T	7: 127,414,575 (GRCm39)	D16E	probably benign	Het
Supv3l1	A	T	10: 62,266,375 (GRCm39)	N600K	possibly damaging	Het
Syne1	C	T	10: 4,991,494 (GRCm39)	V557I	probably benign	Het
Tanc2	T	A	11: 105,667,672 (GRCm39)	D84E	probably damaging	Het
Tep1	A	T	14: 51,105,774 (GRCm39)	L82Q	probably damaging	Het
Tfip11	T	C	5: 112,479,086 (GRCm39)		probably null	Het
Thtpa	G	T	14: 55,333,290 (GRCm39)	R125L	probably damaging	Het
Tm9sf1	C	T	14: 55,880,301 (GRCm39)	G32D	possibly damaging	Het
Tmcc3	A	T	10: 94,415,015 (GRCm39)	N239I	probably damaging	Het
Tpd52	A	T	3: 8,996,255 (GRCm39)		probably null	Het
Traf6	T	A	2: 101,515,100 (GRCm39)	C85*	probably null	Het
Trim68	T	G	7: 102,327,990 (GRCm39)	D321A	probably damaging	Het
Trmt6	C	A	2: 132,650,703 (GRCm39)	A302S	probably benign	Het
Ttbk1	T	C	17: 46,758,558 (GRCm39)	D692G	probably benign	Het
Ttc17	A	T	2: 94,133,985 (GRCm39)	W1067R	probably damaging	Het
Ttc6	T	A	12: 57,689,821 (GRCm39)		probably null	Het
Tut7	A	T	13: 59,937,660 (GRCm39)		probably null	Het
Txnrd2	T	G	16: 18,296,442 (GRCm39)	I468S	probably damaging	Het
Ube2b	A	T	11: 51,879,471 (GRCm39)	Y100N	probably damaging	Het
Ucn3	A	T	13: 3,991,474 (GRCm39)	F59L	probably benign	Het
Uggt1	A	G	1: 36,223,493 (GRCm39)	Y599H	probably damaging	Het
Ulk3	T	A	9: 57,498,023 (GRCm39)	I108N	possibly damaging	Het
Vav3	A	G	3: 109,434,791 (GRCm39)	M441V	possibly damaging	Het
Vmn2r1	T	A	3: 64,008,819 (GRCm39)	D499E	possibly damaging	Het
Vmn2r118	C	T	17: 55,918,565 (GRCm39)	G109D	probably benign	Het
Vmn2r5	T	A	3: 64,416,931 (GRCm39)	M76L	probably benign	Het
Vwa3a	A	G	7: 120,389,365 (GRCm39)	K68E	possibly damaging	Het
Zmiz1	A	T	14: 25,650,237 (GRCm39)	Y459F	probably damaging	Het

Other mutations in Nfkb1

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01293:Nfkb1	APN	3	135,296,600 (GRCm39)	missense	probably damaging	1.00
IGL01345:Nfkb1	APN	3	135,300,742 (GRCm39)	missense	probably damaging	1.00
IGL01629:Nfkb1	APN	3	135,307,228 (GRCm39)	missense	probably benign
IGL02216:Nfkb1	APN	3	135,300,724 (GRCm39)	missense	probably damaging	0.98
IGL02273:Nfkb1	APN	3	135,310,968 (GRCm39)	missense	probably benign	0.01
IGL02508:Nfkb1	APN	3	135,296,579 (GRCm39)	missense	probably damaging	0.99
IGL03095:Nfkb1	APN	3	135,324,591 (GRCm39)	missense	possibly damaging	0.48
Conversely	UTSW	3	135,332,420 (GRCm39)	missense	probably damaging	1.00
Finlay	UTSW	3	135,300,814 (GRCm39)	nonsense	probably null
Frisbee	UTSW	3	135,319,704 (GRCm39)	missense	possibly damaging	0.93
Honeyeater	UTSW	3	135,297,312 (GRCm39)	splice site	probably benign
kookaburra	UTSW	3	135,332,372 (GRCm39)	nonsense	probably null
Murgatroyd	UTSW	3	135,332,471 (GRCm39)	missense	possibly damaging	0.72
Poderoso	UTSW	3	135,319,751 (GRCm39)	missense	probably damaging	1.00
Puff	UTSW	3	135,300,814 (GRCm39)	nonsense	probably null
Roomba	UTSW	3	135,318,173 (GRCm39)	critical splice donor site	probably null
Wheelo	UTSW	3	135,321,110 (GRCm39)	missense	possibly damaging	0.81
R0026:Nfkb1	UTSW	3	135,297,334 (GRCm39)	missense	probably damaging	1.00
R0047:Nfkb1	UTSW	3	135,300,814 (GRCm39)	nonsense	probably null
R0989:Nfkb1	UTSW	3	135,295,157 (GRCm39)	missense	probably benign	0.00
R1210:Nfkb1	UTSW	3	135,300,688 (GRCm39)	missense	probably benign	0.03
R1661:Nfkb1	UTSW	3	135,300,718 (GRCm39)	missense	probably damaging	1.00
R1665:Nfkb1	UTSW	3	135,300,718 (GRCm39)	missense	probably damaging	1.00
R1725:Nfkb1	UTSW	3	135,373,519 (GRCm39)	missense	probably damaging	1.00
R1984:Nfkb1	UTSW	3	135,321,110 (GRCm39)	missense	possibly damaging	0.81
R1985:Nfkb1	UTSW	3	135,321,110 (GRCm39)	missense	possibly damaging	0.81
R2154:Nfkb1	UTSW	3	135,307,240 (GRCm39)	missense	probably benign	0.44
R2281:Nfkb1	UTSW	3	135,307,282 (GRCm39)	missense	probably damaging	1.00
R2409:Nfkb1	UTSW	3	135,319,704 (GRCm39)	missense	possibly damaging	0.93
R2504:Nfkb1	UTSW	3	135,295,090 (GRCm39)	missense	possibly damaging	0.51
R4032:Nfkb1	UTSW	3	135,300,110 (GRCm39)	missense	possibly damaging	0.63
R4232:Nfkb1	UTSW	3	135,309,531 (GRCm39)	missense	probably damaging	1.00
R4936:Nfkb1	UTSW	3	135,319,743 (GRCm39)	missense	probably damaging	0.97
R5085:Nfkb1	UTSW	3	135,309,568 (GRCm39)	missense	probably benign	0.36
R5262:Nfkb1	UTSW	3	135,318,173 (GRCm39)	critical splice donor site	probably null
R5384:Nfkb1	UTSW	3	135,318,303 (GRCm39)	missense	possibly damaging	0.95
R5434:Nfkb1	UTSW	3	135,332,372 (GRCm39)	nonsense	probably null
R5663:Nfkb1	UTSW	3	135,309,612 (GRCm39)	missense	possibly damaging	0.88
R5865:Nfkb1	UTSW	3	135,309,541 (GRCm39)	missense	probably damaging	1.00
R6006:Nfkb1	UTSW	3	135,309,522 (GRCm39)	nonsense	probably null
R6013:Nfkb1	UTSW	3	135,332,445 (GRCm39)	missense	possibly damaging	0.86
R6234:Nfkb1	UTSW	3	135,332,471 (GRCm39)	missense	possibly damaging	0.72
R6785:Nfkb1	UTSW	3	135,321,064 (GRCm39)	missense	probably benign
R7175:Nfkb1	UTSW	3	135,319,751 (GRCm39)	missense	probably damaging	1.00
R7227:Nfkb1	UTSW	3	135,332,420 (GRCm39)	missense	probably damaging	1.00
R7394:Nfkb1	UTSW	3	135,319,458 (GRCm39)	missense	possibly damaging	0.54
R7727:Nfkb1	UTSW	3	135,291,162 (GRCm39)	missense	possibly damaging	0.48
R7815:Nfkb1	UTSW	3	135,309,552 (GRCm39)	missense	probably damaging	1.00
R7849:Nfkb1	UTSW	3	135,291,173 (GRCm39)	missense
R8004:Nfkb1	UTSW	3	135,297,312 (GRCm39)	splice site	probably benign
R8059:Nfkb1	UTSW	3	135,299,613 (GRCm39)	missense	possibly damaging	0.54
R8806:Nfkb1	UTSW	3	135,295,213 (GRCm39)	missense	probably damaging	1.00
R9169:Nfkb1	UTSW	3	135,310,874 (GRCm39)	missense	probably benign	0.00
X0050:Nfkb1	UTSW	3	135,312,384 (GRCm39)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- CTTTCAGAGCAAGCACAAGAG -3'
(R):5'- AGAAATGGCTGAGTCGCGTG -3'

Sequencing Primer
(F):5'- TTCAGAGCAAGCACAAGAGTCTAAAC -3'
(R):5'- TACTTTCACGGGGGCAGTATAAG -3'

Posted On

2016-08-04