Mutagenetix > Incidental Mutation

Incidental Mutation 'R5391:Or6d15'

425847

Institutional Source

Beutler Lab

Gene Symbol

Or6d15

Ensembl Gene

ENSMUSG00000050654

Gene Name

olfactory receptor family 6 subfamily D member 15

Synonyms

GA_x54KRFPKN04-58217732-58216800, MOR119-2, Olfr215

MMRRC Submission

042963-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.078) question?

Stock #

R5391 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

116558973-116559905 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 116559808 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Tyrosine to Cysteine at position 33 (Y33C)

Ref Sequence

ENSEMBL: ENSMUSP00000052425 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000061723]

AlphaFold

Q8VF82

Predicted Effect

probably damaging
Transcript: ENSMUST00000061723
AA Change: Y33C

PolyPhen 2 Score 0.999 (Sensitivity: 0.14; Specificity: 0.99)

SMART Domains

Protein: ENSMUSP00000052425
Gene: ENSMUSG00000050654
AA Change: Y33C

Domain	Start	End	E-Value	Type
Pfam:7tm_4	29	306	5e-47	PFAM
Pfam:7TM_GPCR_Srsx	33	258	1.8e-5	PFAM
Pfam:7tm_1	39	288	2.9e-15	PFAM

Coding Region Coverage

1x: 99.3%
3x: 98.7%
10x: 97.4%
20x: 95.6%

Validation Efficiency

MGI Phenotype

FUNCTION: Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]

Allele List at MGI

Other mutations in this stock

Total: 51 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Abcb1b	G	A	5: 8,855,481 (GRCm39)	M38I	probably null	Het
Actl7a	A	G	4: 56,743,661 (GRCm39)	T63A	probably benign	Het
Amfr	G	A	8: 94,702,676 (GRCm39)	P497S	probably damaging	Het
Ankrd33b	T	C	15: 31,325,352 (GRCm39)	I122V	probably damaging	Het
Asap1	G	T	15: 63,965,901 (GRCm39)	T1011K	possibly damaging	Het
Cbfa2t3	G	A	8: 123,360,134 (GRCm39)	R506*	probably null	Het
Ccs	C	G	19: 4,883,510 (GRCm39)	C96S	probably benign	Het
Cpt1a	A	G	19: 3,399,260 (GRCm39)	D20G	probably damaging	Het
Ctdspl2	G	A	2: 121,834,629 (GRCm39)		probably null	Het
Dhx57	T	C	17: 80,582,510 (GRCm39)	Y365C	probably damaging	Het
Dnah3	C	T	7: 119,689,299 (GRCm39)	M38I	probably benign	Het
Dnajc6	T	C	4: 101,485,355 (GRCm39)		probably null	Het
Elac2	A	G	11: 64,885,120 (GRCm39)	S450G	probably benign	Het
Gdf9	T	C	11: 53,324,624 (GRCm39)	V131A	probably benign	Het
Il12rb2	T	C	6: 67,269,404 (GRCm39)	N803S	probably benign	Het
Itgb4	T	A	11: 115,875,894 (GRCm39)	M477K	probably benign	Het
Itgb8	A	C	12: 119,134,476 (GRCm39)	C530W	probably damaging	Het
Krt78	C	A	15: 101,860,263 (GRCm39)	E218*	probably null	Het
Lpar1	A	G	4: 58,486,902 (GRCm39)	L105P	probably damaging	Het
Megf8	G	A	7: 25,039,714 (GRCm39)	G936D	possibly damaging	Het
Mov10	G	A	3: 104,709,849 (GRCm39)	H346Y	probably benign	Het
Nfia	A	G	4: 97,671,538 (GRCm39)	I83V	probably damaging	Het
Or5b101	G	T	19: 13,005,150 (GRCm39)	A181E	probably damaging	Het
Pcdhgb6	T	G	18: 37,875,640 (GRCm39)	I116S	probably damaging	Het
Pdcd6ip	G	T	9: 113,520,586 (GRCm39)	Q133K	probably damaging	Het
Phkb	A	G	8: 86,744,097 (GRCm39)	D582G	probably damaging	Het
Pik3cd	A	T	4: 149,743,588 (GRCm39)	V207E	probably damaging	Het
Ptov1	T	C	7: 44,513,008 (GRCm39)	Q397R	probably damaging	Het
Rangap1	A	G	15: 81,590,647 (GRCm39)	F482L	probably benign	Het
Rapgef1	T	A	2: 29,627,977 (GRCm39)	N1052K	probably damaging	Het
Rasl12	G	T	9: 65,305,949 (GRCm39)	A35S	probably damaging	Het
Rnf169	A	T	7: 99,584,367 (GRCm39)		probably null	Het
Sec16a	A	G	2: 26,330,044 (GRCm39)	V657A	possibly damaging	Het
Sin3a	G	A	9: 57,012,957 (GRCm39)	R612H	probably damaging	Het
Six6	T	A	12: 72,988,475 (GRCm39)	L216*	probably null	Het
Tbce	T	C	13: 14,180,550 (GRCm39)	I293M	probably damaging	Het
Tektl1	G	A	10: 78,588,688 (GRCm39)	Q41*	probably null	Het
Tmem176a	T	C	6: 48,821,630 (GRCm39)	L204P	probably damaging	Het
Tmem87a	A	G	2: 120,193,358 (GRCm39)		probably null	Het
Tns1	A	T	1: 74,029,568 (GRCm39)		probably null	Het
Usf3	T	A	16: 44,037,826 (GRCm39)	S769T	probably benign	Het
Vmn2r82	A	G	10: 79,192,491 (GRCm39)	T23A	probably null	Het
Vps26a	A	G	10: 62,292,526 (GRCm39)	*328Q	probably null	Het
Vps51	T	G	19: 6,121,063 (GRCm39)	E283D	probably benign	Het
Wwc2	A	T	8: 48,316,906 (GRCm39)	I729K	unknown	Het
Zbtb44	A	G	9: 30,964,601 (GRCm39)		probably null	Het
Zfp800	A	T	6: 28,242,992 (GRCm39)	S658T	probably damaging	Het
Zfp825	T	C	13: 74,628,665 (GRCm39)	T284A	possibly damaging	Het
Zfp935	G	T	13: 62,602,632 (GRCm39)	Y189*	probably null	Het
Zkscan1	T	A	5: 138,095,363 (GRCm39)	H203Q	probably benign	Het
Zkscan14	T	C	5: 145,132,604 (GRCm39)	D309G	probably benign	Het

Other mutations in Or6d15

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01666:Or6d15	APN	6	116,559,296 (GRCm39)	missense	possibly damaging	0.93
IGL02959:Or6d15	APN	6	116,559,505 (GRCm39)	missense	probably damaging	1.00
IGL03053:Or6d15	APN	6	116,559,206 (GRCm39)	missense	possibly damaging	0.50
R0078:Or6d15	UTSW	6	116,559,701 (GRCm39)	missense	probably damaging	0.96
R0277:Or6d15	UTSW	6	116,559,562 (GRCm39)	missense	probably damaging	0.99
R0323:Or6d15	UTSW	6	116,559,562 (GRCm39)	missense	probably damaging	0.99
R0399:Or6d15	UTSW	6	116,559,742 (GRCm39)	missense	probably benign	0.00
R0545:Or6d15	UTSW	6	116,559,617 (GRCm39)	missense	probably benign	0.01
R1213:Or6d15	UTSW	6	116,559,827 (GRCm39)	missense	probably benign	0.00
R1775:Or6d15	UTSW	6	116,559,925 (GRCm39)	start gained	probably benign
R1789:Or6d15	UTSW	6	116,559,658 (GRCm39)	missense	probably damaging	1.00
R4724:Or6d15	UTSW	6	116,559,898 (GRCm39)	missense	probably damaging	1.00
R5392:Or6d15	UTSW	6	116,559,379 (GRCm39)	missense	probably damaging	1.00
R5686:Or6d15	UTSW	6	116,559,890 (GRCm39)	missense	probably benign	0.00
R6124:Or6d15	UTSW	6	116,559,446 (GRCm39)	missense	probably benign	0.05
R7080:Or6d15	UTSW	6	116,559,314 (GRCm39)	missense	probably damaging	1.00
R7355:Or6d15	UTSW	6	116,559,916 (GRCm39)	start gained	probably benign
R9084:Or6d15	UTSW	6	116,559,232 (GRCm39)	missense	probably benign	0.03
Z1177:Or6d15	UTSW	6	116,559,514 (GRCm39)	missense	probably benign	0.01

Predicted Primers

PCR Primer
(F):5'- CCGAATGCCCCGATAAATAGAG -3'
(R):5'- GAATGACCTCATTGTGTGCAC -3'

Sequencing Primer
(F):5'- AGGTTTGTATGAAACAGGCAAC -3'
(R):5'- GTGCACTGTGAGAATACCTCTGC -3'

Posted On

2016-08-04