Mutagenetix > Incidental Mutation

Incidental Mutation 'R5409:Vmn1r65'

426447

Institutional Source

Beutler Lab

Gene Symbol

Vmn1r65

Ensembl Gene

ENSMUSG00000066850

Gene Name

vomeronasal 1 receptor 65

Synonyms

V1rd6, V3R6

MMRRC Submission

042978-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.054) question?

Stock #

R5409 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

6010749-6014009 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 6012012 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Asparagine to Serine at position 74 (N74S)

Ref Sequence

ENSEMBL: ENSMUSP00000083520 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000086338]

AlphaFold

Q9EPS7

Predicted Effect

possibly damaging
Transcript: ENSMUST00000086338
AA Change: N74S

PolyPhen 2 Score 0.775 (Sensitivity: 0.85; Specificity: 0.92)

SMART Domains

Protein: ENSMUSP00000083520
Gene: ENSMUSG00000066850
AA Change: N74S

Domain	Start	End	E-Value	Type
Pfam:TAS2R	20	308	7.3e-14	PFAM
Pfam:7tm_1	42	301	1.6e-6	PFAM
Pfam:V1R	53	308	5.6e-15	PFAM

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000227656

Coding Region Coverage

1x: 99.2%
3x: 98.6%
10x: 97.1%
20x: 94.8%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 38 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Abca7	T	C	10: 79,850,154 (GRCm39)	L2002P	probably damaging	Het
Acot11	C	T	4: 106,617,327 (GRCm39)	G240R	probably damaging	Het
Adgrl1	A	G	8: 84,656,371 (GRCm39)	T230A	probably damaging	Het
Anapc4	A	G	5: 53,005,941 (GRCm39)	E316G	probably damaging	Het
Asic1	GCACC	GCACCACC	15: 99,596,684 (GRCm39)		probably benign	Het
Aurka	T	G	2: 172,209,036 (GRCm39)	Q33P	possibly damaging	Het
Ccn5	G	A	2: 163,667,158 (GRCm39)	C53Y	probably damaging	Het
Cenpm	T	C	15: 82,118,564 (GRCm39)	T153A	probably benign	Het
Clca4b	T	A	3: 144,622,452 (GRCm39)	K538*	probably null	Het
Clip2	G	A	5: 134,551,645 (GRCm39)	T159M	possibly damaging	Het
Col5a1	C	T	2: 27,850,457 (GRCm39)	T518I	unknown	Het
Dis3	A	T	14: 99,323,368 (GRCm39)	M566K	possibly damaging	Het
Dnah1	C	T	14: 30,985,212 (GRCm39)	R3869H	probably damaging	Het
Gm4775	A	T	14: 106,338,386 (GRCm39)		noncoding transcript	Het
Hipk2	C	T	6: 38,706,977 (GRCm39)	G637D	probably damaging	Het
Igkv4-61	T	C	6: 69,394,111 (GRCm39)	K18E	possibly damaging	Het
Kcnk4	A	G	19: 6,903,578 (GRCm39)	S324P	probably benign	Het
Larp4	T	C	15: 99,883,945 (GRCm39)	C61R	probably damaging	Het
Nid2	T	C	14: 19,856,030 (GRCm39)	F986L	probably damaging	Het
Or5ac16	A	G	16: 59,021,920 (GRCm39)	Y290H	probably damaging	Het
Or5aq1b	T	C	2: 86,902,214 (GRCm39)	E88G	possibly damaging	Het
Or5h22	C	T	16: 58,894,559 (GRCm39)	V295I	possibly damaging	Het
Pgbd5	T	A	8: 125,098,619 (GRCm39)	I359F	probably damaging	Het
Plekhh2	T	C	17: 84,893,906 (GRCm39)		probably null	Het
Pomgnt2	A	T	9: 121,811,303 (GRCm39)	S493T	possibly damaging	Het
Rnf7l	A	T	10: 63,257,403 (GRCm39)	M39K	possibly damaging	Het
Rp1l1	T	C	14: 64,268,070 (GRCm39)	S1219P	probably benign	Het
Rprd1b	T	A	2: 157,916,987 (GRCm39)	F322L	probably damaging	Het
Sh3rf1	G	A	8: 61,827,279 (GRCm39)	V678M	probably benign	Het
Smpd5	C	A	15: 76,179,914 (GRCm39)	T321K	probably damaging	Het
Spag8	T	A	4: 43,653,134 (GRCm39)		probably benign	Het
Tanc2	T	C	11: 105,758,311 (GRCm39)	C691R	possibly damaging	Het
Tnc	A	C	4: 63,884,773 (GRCm39)	M1834R	probably damaging	Het
Tnc	A	T	4: 63,925,654 (GRCm39)	Y961N	probably damaging	Het
Ttn	T	C	2: 76,700,893 (GRCm39)		probably benign	Het
Ufl1	A	G	4: 25,280,706 (GRCm39)	V47A	probably damaging	Het
Unc13c	T	A	9: 73,485,672 (GRCm39)	D1676V	possibly damaging	Het
Vmn2r30	A	G	7: 7,315,547 (GRCm39)	F762S	probably damaging	Het

Other mutations in Vmn1r65

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01636:Vmn1r65	APN	7	6,011,720 (GRCm39)	missense	probably benign	0.12
IGL02821:Vmn1r65	APN	7	6,011,893 (GRCm39)	missense	possibly damaging	0.62
R0991:Vmn1r65	UTSW	7	6,012,076 (GRCm39)	missense	probably damaging	0.99
R1457:Vmn1r65	UTSW	7	6,012,156 (GRCm39)	missense	probably benign	0.10
R1507:Vmn1r65	UTSW	7	6,012,108 (GRCm39)	missense	probably benign	0.13
R1856:Vmn1r65	UTSW	7	6,011,265 (GRCm39)	missense	possibly damaging	0.93
R2914:Vmn1r65	UTSW	7	6,012,040 (GRCm39)	missense	possibly damaging	0.69
R5147:Vmn1r65	UTSW	7	6,011,818 (GRCm39)	missense	probably benign	0.01
R5279:Vmn1r65	UTSW	7	6,011,754 (GRCm39)	missense	probably damaging	1.00
R5399:Vmn1r65	UTSW	7	6,011,809 (GRCm39)	nonsense	probably null
R5646:Vmn1r65	UTSW	7	6,012,223 (GRCm39)	missense	probably benign	0.01
R5963:Vmn1r65	UTSW	7	6,011,608 (GRCm39)	missense	probably damaging	1.00
R6034:Vmn1r65	UTSW	7	6,011,868 (GRCm39)	missense	probably damaging	1.00
R6034:Vmn1r65	UTSW	7	6,011,868 (GRCm39)	missense	probably damaging	1.00
R6327:Vmn1r65	UTSW	7	6,011,651 (GRCm39)	missense	possibly damaging	0.48
R7958:Vmn1r65	UTSW	7	6,011,254 (GRCm39)	missense	probably benign	0.01
R8121:Vmn1r65	UTSW	7	6,011,464 (GRCm39)	missense	possibly damaging	0.87
R8345:Vmn1r65	UTSW	7	6,011,256 (GRCm39)	missense	probably benign
R8725:Vmn1r65	UTSW	7	6,011,503 (GRCm39)	missense	probably damaging	1.00
R8727:Vmn1r65	UTSW	7	6,011,503 (GRCm39)	missense	probably damaging	1.00
R9246:Vmn1r65	UTSW	7	6,011,769 (GRCm39)	missense	possibly damaging	0.95
R9279:Vmn1r65	UTSW	7	6,011,988 (GRCm39)	missense	probably benign	0.06
R9778:Vmn1r65	UTSW	7	6,011,387 (GRCm39)	nonsense	probably null

Predicted Primers

PCR Primer
(F):5'- TGAAGATGACCCTAGCCCAG -3'
(R):5'- AATGAAGCCATCTCCACGTTGTC -3'

Sequencing Primer
(F):5'- GATGACCCTAGCCCAGGTACC -3'
(R):5'- GAATTCTGGGCAACATCC -3'

Posted On

2016-09-01