Mutagenetix > Incidental Mutation

Incidental Mutation 'R5441:Or5m13'

427161

Institutional Source

Beutler Lab

Gene Symbol

Or5m13

Ensembl Gene

ENSMUSG00000058884

Gene Name

olfactory receptor family 5 subfamily M member 13

Synonyms

MOR196-5P, GA_x6K02T2Q125-47397266-47398205, Olfr1025, MOR196-6_p

MMRRC Submission

043006-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.099) question?

Stock #

R5441 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

85748271-85749097 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to A at 85748934 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Phenylalanine to Isoleucine at position 222 (F222I)

Ref Sequence

ENSEMBL: ENSMUSP00000149785 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000056718] [ENSMUST00000188749] [ENSMUST00000213474] [ENSMUST00000217615] [ENSMUST00000219615]

AlphaFold

A0A140T8K3

Predicted Effect

probably benign
Transcript: ENSMUST00000056718

SMART Domains

Protein: ENSMUSP00000049887
Gene: ENSMUSG00000042863

Domain	Start	End	E-Value	Type
Pfam:7tm_4	31	307	6.1e-58	PFAM
Pfam:7tm_1	41	290	9e-25	PFAM

Predicted Effect

SMART Domains

Protein: ENSMUSP00000076311
Gene: ENSMUSG00000058884
AA Change: F222I

Domain	Start	End	E-Value	Type
Pfam:7tm_4	31	308	1.5e-57	PFAM
Pfam:7tm_1	41	290	2.6e-24	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000188749
AA Change: F222I

PolyPhen 2 Score 0.007 (Sensitivity: 0.96; Specificity: 0.75)

SMART Domains

Protein: ENSMUSP00000139860
Gene: ENSMUSG00000058884
AA Change: F222I

Domain	Start	End	E-Value	Type
low complexity region	26	40	N/A	INTRINSIC
Pfam:7tm_1	41	290	5.7e-30	PFAM
Pfam:7tm_4	139	283	7.8e-48	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000213474
AA Change: F222I

PolyPhen 2 Score 0.007 (Sensitivity: 0.96; Specificity: 0.75)

Predicted Effect

probably benign
Transcript: ENSMUST00000217615

Predicted Effect

probably benign
Transcript: ENSMUST00000219615

Meta Mutation Damage Score

0.0898

Coding Region Coverage

1x: 99.2%
3x: 98.6%
10x: 97.1%
20x: 95.1%

Validation Efficiency

98% (56/57)

Allele List at MGI

Other mutations in this stock

Total: 55 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Abca12	A	G	1: 71,334,215 (GRCm39)	Y1096H	probably damaging	Het
Aspa	A	T	11: 73,196,420 (GRCm39)	F261I	probably damaging	Het
Atm	C	A	9: 53,427,767 (GRCm39)	G448*	probably null	Het
Atmin	T	A	8: 117,684,696 (GRCm39)	D785E	probably damaging	Het
Car1	G	C	3: 14,841,364 (GRCm39)	R90G	probably damaging	Het
Cdh11	T	C	8: 103,374,178 (GRCm39)	D520G	probably benign	Het
Cers5	T	A	15: 99,649,119 (GRCm39)	K50*	probably null	Het
Chad	A	T	11: 94,459,118 (GRCm39)	D340V	probably benign	Het
Cspg5	T	C	9: 110,075,711 (GRCm39)	I68T	probably benign	Het
Fcgbpl1	C	T	7: 27,856,339 (GRCm39)	T2042M	probably damaging	Het
Fcrla	T	A	1: 170,752,991 (GRCm39)		probably benign	Het
Fer1l4	T	A	2: 155,865,177 (GRCm39)	D1608V	probably benign	Het
Fzd5	T	A	1: 64,774,576 (GRCm39)	Q395L	probably benign	Het
Gm7664	T	A	13: 62,676,464 (GRCm39)		probably benign	Het
Hk3	T	C	13: 55,162,869 (GRCm39)	E2G	probably damaging	Het
Hmcn2	G	A	2: 31,296,428 (GRCm39)	E2677K	possibly damaging	Het
Hydin	T	C	8: 111,291,741 (GRCm39)	L3411P	possibly damaging	Het
Ltbr	G	A	6: 125,289,757 (GRCm39)	R146W	probably damaging	Het
Lysmd2	C	T	9: 75,533,254 (GRCm39)	H70Y	possibly damaging	Het
Msi2	A	G	11: 88,370,818 (GRCm39)		probably benign	Het
Msi2	G	A	11: 88,608,921 (GRCm39)		probably benign	Het
Napsa	T	A	7: 44,230,817 (GRCm39)		probably benign	Het
Nlrp4a	T	A	7: 26,153,578 (GRCm39)	L710M	probably damaging	Het
Or10g1	T	A	14: 52,647,414 (GRCm39)	K305M	probably benign	Het
Or12d13	T	C	17: 37,647,159 (GRCm39)		probably null	Het
Or6ae1	T	C	7: 139,742,564 (GRCm39)	T100A	probably benign	Het
Or9a2	C	T	6: 41,748,782 (GRCm39)	M150I	probably benign	Het
Plbd2	T	A	5: 120,637,147 (GRCm39)	Y105F	probably benign	Het
Plppr5	A	T	3: 117,456,120 (GRCm39)	I214F	possibly damaging	Het
Pramel34	A	T	5: 93,784,456 (GRCm39)	M139K	possibly damaging	Het
Ptcd3	A	G	6: 71,858,505 (GRCm39)	V655A	possibly damaging	Het
Ptpn14	T	C	1: 189,530,767 (GRCm39)	L78P	probably damaging	Het
Ralgapa1	A	C	12: 55,766,408 (GRCm39)	D1295E	probably damaging	Het
Rnf213	G	A	11: 119,299,846 (GRCm39)	D192N	probably damaging	Het
Ropn1	A	G	16: 34,487,167 (GRCm39)	I34M	probably damaging	Het
Rpe65	G	T	3: 159,310,038 (GRCm39)	G104C	probably damaging	Het
Scgb2b27	T	C	7: 33,712,582 (GRCm39)		probably benign	Het
Sh3bgr	T	C	16: 96,007,117 (GRCm39)	I29T	possibly damaging	Het
Smg1	T	C	7: 117,794,304 (GRCm39)		probably benign	Het
Stim2	T	A	5: 54,232,712 (GRCm39)	C68*	probably null	Het
Syne2	G	A	12: 76,035,917 (GRCm39)	V3736I	possibly damaging	Het
Tbx19	T	C	1: 164,981,249 (GRCm39)	N82D	probably damaging	Het
Tdp1	T	A	12: 99,876,544 (GRCm39)	V353D	probably damaging	Het
Tg	T	A	15: 66,568,369 (GRCm39)	I1352K	possibly damaging	Het
Thsd4	T	C	9: 59,887,066 (GRCm39)	T919A	probably damaging	Het
Tmprss11f	A	T	5: 86,676,062 (GRCm39)	M373K	probably damaging	Het
Tmprss15	T	A	16: 78,868,335 (GRCm39)		probably null	Het
Tpd52	A	T	3: 9,068,466 (GRCm39)	Y16*	probably null	Het
Ube2o	G	A	11: 116,435,268 (GRCm39)	R507C	probably damaging	Het
Unc93b1	T	C	19: 3,993,703 (GRCm39)	F382L	probably benign	Het
Vav2	A	T	2: 27,160,122 (GRCm39)		probably benign	Het
Vmn1r207	T	C	13: 22,910,686 (GRCm39)		noncoding transcript	Het
Zan	T	A	5: 137,435,013 (GRCm39)	I2127F	unknown	Het
Zfp748	C	A	13: 67,688,737 (GRCm39)	C841F	probably damaging	Het
Zic4	C	A	9: 91,266,253 (GRCm39)	P299Q	probably damaging	Het

Other mutations in Or5m13

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01660:Or5m13	APN	2	85,748,908 (GRCm39)	missense	probably benign	0.25
IGL02881:Or5m13	APN	2	85,748,460 (GRCm39)	missense	probably benign	0.00
R0097:Or5m13	UTSW	2	85,749,184 (GRCm39)	missense	probably benign	0.00
R0097:Or5m13	UTSW	2	85,749,184 (GRCm39)	missense	probably benign	0.00
R0324:Or5m13	UTSW	2	85,748,295 (GRCm39)	missense	probably benign	0.22
R0392:Or5m13	UTSW	2	85,749,106 (GRCm39)	missense	possibly damaging	0.81
R0765:Or5m13	UTSW	2	85,749,049 (GRCm39)	missense	probably damaging	0.99
R1199:Or5m13	UTSW	2	85,748,379 (GRCm39)	missense	probably benign	0.00
R1894:Or5m13	UTSW	2	85,748,599 (GRCm39)	missense	probably benign	0.28
R2027:Or5m13	UTSW	2	85,749,114 (GRCm39)	missense	probably damaging	0.99
R2141:Or5m13	UTSW	2	85,749,171 (GRCm39)	missense	probably null
R3871:Or5m13	UTSW	2	85,748,926 (GRCm39)	splice site	probably null
R4837:Or5m13	UTSW	2	85,748,748 (GRCm39)	missense	probably benign	0.00
R4945:Or5m13	UTSW	2	85,748,917 (GRCm39)	missense	possibly damaging	0.81
R5056:Or5m13	UTSW	2	85,748,480 (GRCm39)	missense	probably damaging	1.00
R5960:Or5m13	UTSW	2	85,749,069 (GRCm39)	missense	probably benign	0.07
R6807:Or5m13	UTSW	2	85,748,382 (GRCm39)	missense	possibly damaging	0.48
R7320:Or5m13	UTSW	2	85,748,718 (GRCm39)	missense	probably benign	0.00
R8523:Or5m13	UTSW	2	85,748,407 (GRCm39)	missense	probably damaging	0.97
R8715:Or5m13	UTSW	2	85,748,273 (GRCm39)	start codon destroyed	probably null	0.81
R8734:Or5m13	UTSW	2	85,748,993 (GRCm39)	nonsense	probably null
R9275:Or5m13	UTSW	2	85,749,046 (GRCm39)	missense	probably benign	0.17
R9427:Or5m13	UTSW	2	85,748,704 (GRCm39)	missense	probably benign	0.00
R9728:Or5m13	UTSW	2	85,748,887 (GRCm39)	missense	possibly damaging	0.72
V1662:Or5m13	UTSW	2	85,748,938 (GRCm39)	missense	probably benign	0.10
Z1176:Or5m13	UTSW	2	85,748,845 (GRCm39)	missense	probably damaging	0.99

Predicted Primers

PCR Primer
(F):5'- ATTTGCAAATGGCCTGGCAC -3'
(R):5'- GGTTCAACATCGGGCTTACAAAG -3'

Sequencing Primer
(F):5'- TGGCACAGACCATCCTGATG -3'
(R):5'- TCGGGCTTACAAAGATATAAAAGACC -3'

Posted On

2016-09-01