Mutagenetix > Incidental Mutation

Incidental Mutation 'R5435:Wee1'

428260

Institutional Source

Beutler Lab

Gene Symbol

Wee1

Ensembl Gene

ENSMUSG00000031016

Gene Name

WEE 1 homolog 1 (S. pombe)

Synonyms

Wee1A

MMRRC Submission

043000-MU

Accession Numbers

Essential gene?

Essential (E-score: 1.000) question?

Stock #

R5435 (G1)

Quality Score

175

Status

Validated

Chromosome

Chromosomal Location

109721266-109742506 bp(+) (GRCm39)

Type of Mutation

frame shift

DNA Base Change (assembly)

TCCCC to TCCC at 109723776 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Ref Sequence

ENSEMBL: ENSMUSP00000033326 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000033326]

AlphaFold

P47810

Predicted Effect

probably null
Transcript: ENSMUST00000033326

SMART Domains

Protein: ENSMUSP00000033326
Gene: ENSMUSG00000031016

Domain	Start	End	E-Value	Type
low complexity region	62	112	N/A	INTRINSIC
Pfam:Pkinase_Tyr	298	566	1.9e-26	PFAM
Pfam:Pkinase	298	568	1.7e-61	PFAM
low complexity region	620	631	N/A	INTRINSIC

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000185931

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000210940

Meta Mutation Damage Score

0.9755

Coding Region Coverage

1x: 99.2%
3x: 98.6%
10x: 97.1%
20x: 94.9%

Validation Efficiency

100% (74/74)

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes a nuclear protein, which is a tyrosine kinase belonging to the Ser/Thr family of protein kinases. This protein catalyzes the inhibitory tyrosine phosphorylation of CDC2/cyclin B kinase, and appears to coordinate the transition between DNA replication and mitosis by protecting the nucleus from cytoplasmically activated CDC2 kinase. [provided by RefSeq, Jul 2008]
PHENOTYPE: Mice homozygous for a transgenic gene disruption may exhibit embryonic lethality at E7. Mice homozygous for a knock-out allele exhibit lethality between E3.5 and E7.5 with reduced proliferation, increased apoptosis and abnormal G2/M checkpoint function. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 64 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
3425401B19Rik	A	G	14: 32,383,413 (GRCm39)	F851L	probably benign	Het
Abca17	C	T	17: 24,486,588 (GRCm39)	V1480I	possibly damaging	Het
Acsbg1	G	T	9: 54,523,153 (GRCm39)	Y491*	probably null	Het
Acsf3	T	A	8: 123,507,020 (GRCm39)	N104K	probably damaging	Het
Adam23	T	C	1: 63,585,612 (GRCm39)	Y400H	possibly damaging	Het
Adgra3	G	A	5: 50,147,468 (GRCm39)	T524M	probably damaging	Het
Aff1	G	A	5: 103,902,198 (GRCm39)		probably benign	Het
Anxa9	T	C	3: 95,204,561 (GRCm39)	Y321C	probably damaging	Het
Ap1g1	T	A	8: 110,565,552 (GRCm39)	Y329N	probably damaging	Het
Aph1c	A	T	9: 66,741,783 (GRCm39)	I33N	possibly damaging	Het
B3galnt2	A	G	13: 14,171,575 (GRCm39)	E491G	probably benign	Het
Bdh1	C	T	16: 31,275,475 (GRCm39)	R235C	probably damaging	Het
Ccar2	A	G	14: 70,376,776 (GRCm39)	L856P	probably damaging	Het
Ccdc107	A	T	4: 43,493,519 (GRCm39)	D30V	probably damaging	Het
Ccdc116	G	T	16: 16,960,626 (GRCm39)	H64N	probably benign	Het
Ccl12	T	C	11: 81,994,001 (GRCm39)	I86T	possibly damaging	Het
Col2a1	T	C	15: 97,898,391 (GRCm39)		probably benign	Het
Col4a4	T	A	1: 82,431,728 (GRCm39)	I1519F	unknown	Het
Ddx19b	T	C	8: 111,735,458 (GRCm39)	Q416R	possibly damaging	Het
Dnah6	T	A	6: 73,037,121 (GRCm39)	M3374L	probably benign	Het
Dnajc6	A	T	4: 101,463,807 (GRCm39)	I119F	probably damaging	Het
Ensa	C	A	3: 95,529,769 (GRCm39)		probably benign	Het
Fbxo4	C	T	15: 3,995,274 (GRCm39)	V357I	possibly damaging	Het
Fkbpl	G	A	17: 34,864,303 (GRCm39)	A24T	probably benign	Het
Foxf1	G	A	8: 121,811,231 (GRCm39)	G32S	probably damaging	Het
Gls2	G	A	10: 128,030,995 (GRCm39)		probably benign	Het
Gm13599	T	A	2: 67,226,496 (GRCm39)		noncoding transcript	Het
Gmnn	A	G	13: 24,936,084 (GRCm39)	S197P	probably benign	Het
Guf1	A	T	5: 69,720,512 (GRCm39)	H324L	probably benign	Het
H2-Q6	A	G	17: 35,644,661 (GRCm39)	D150G	probably damaging	Het
Herc3	T	A	6: 58,832,791 (GRCm39)	L152Q	probably damaging	Het
Hnrnpul2	T	G	19: 8,797,682 (GRCm39)	S13A	probably benign	Het
Ighv5-4	A	G	12: 113,561,283 (GRCm39)	F46L	probably benign	Het
Kank1	T	G	19: 25,388,507 (GRCm39)	S727A	probably benign	Het
Kcnma1	A	T	14: 23,578,472 (GRCm39)	Y201*	probably null	Het
Lyst	A	T	13: 13,951,649 (GRCm39)	H3750L	possibly damaging	Het
Mettl25	A	G	10: 105,615,447 (GRCm39)		probably null	Het
Mpdz	A	G	4: 81,201,724 (GRCm39)		probably benign	Het
Myh9	C	T	15: 77,653,809 (GRCm39)	V1280I	probably benign	Het
Neto1	A	T	18: 86,416,388 (GRCm39)	T32S	probably benign	Het
Nol7	C	A	13: 43,554,848 (GRCm39)	H187Q	possibly damaging	Het
Or5ak22	T	A	2: 85,230,814 (GRCm39)	N21I	probably benign	Het
Pcdha8	A	G	18: 37,126,652 (GRCm39)	D378G	probably damaging	Het
Peak1	A	G	9: 56,113,770 (GRCm39)	S694P	probably damaging	Het
Pih1d1	T	A	7: 44,805,696 (GRCm39)		probably null	Het
Pik3c2g	T	G	6: 139,661,581 (GRCm39)		probably null	Het
Prkar2a	A	G	9: 108,617,682 (GRCm39)	R247G	probably damaging	Het
Psg26	A	G	7: 18,212,398 (GRCm39)	I319T	possibly damaging	Het
R3hdm4	C	T	10: 79,748,292 (GRCm39)	E162K	possibly damaging	Het
Rasa3	T	C	8: 13,681,811 (GRCm39)	E46G	possibly damaging	Het
Rbbp5	T	A	1: 132,422,013 (GRCm39)	H304Q	probably damaging	Het
Relch	T	A	1: 105,668,975 (GRCm39)		probably benign	Het
Scn1a	T	A	2: 66,103,878 (GRCm39)	E1783V	probably damaging	Het
Stag1	A	G	9: 100,835,603 (GRCm39)	N151S	probably benign	Het
Tbc1d32	C	A	10: 55,916,246 (GRCm39)	A1191S	probably damaging	Het
Tchh	C	A	3: 93,350,979 (GRCm39)	R140S	possibly damaging	Het
Trank1	A	G	9: 111,220,958 (GRCm39)	Y2565C	probably benign	Het
Ttn	A	T	2: 76,744,702 (GRCm39)	V5449D	probably damaging	Het
Tubgcp2	G	A	7: 139,575,985 (GRCm39)	P893S	possibly damaging	Het
Wdfy4	A	G	14: 32,742,268 (GRCm39)	F2325S	probably damaging	Het
Wdr35	A	G	12: 9,039,951 (GRCm39)	D352G	probably benign	Het
Ypel3	A	G	7: 126,374,960 (GRCm39)		probably benign	Het
Zan	T	A	5: 137,402,024 (GRCm39)	T4023S	unknown	Het
Zfp735	T	A	11: 73,602,939 (GRCm39)	C628S	possibly damaging	Het

Other mutations in Wee1

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00693:Wee1	APN	7	109,734,060 (GRCm39)	splice site	probably null
IGL00981:Wee1	APN	7	109,738,876 (GRCm39)	missense	probably damaging	1.00
IGL01017:Wee1	APN	7	109,725,055 (GRCm39)	missense	possibly damaging	0.93
IGL01357:Wee1	APN	7	109,741,242 (GRCm39)	missense	probably benign	0.39
IGL01838:Wee1	APN	7	109,723,744 (GRCm39)	missense	probably benign	0.01
IGL01970:Wee1	APN	7	109,738,457 (GRCm39)	missense	probably damaging	1.00
IGL02396:Wee1	APN	7	109,741,300 (GRCm39)	missense	probably damaging	1.00
IGL02511:Wee1	APN	7	109,738,483 (GRCm39)	missense	possibly damaging	0.55
IGL02884:Wee1	APN	7	109,725,269 (GRCm39)	missense	probably benign	0.02
IGL03085:Wee1	APN	7	109,723,805 (GRCm39)	missense	probably damaging	1.00
IGL03221:Wee1	APN	7	109,726,024 (GRCm39)	missense	probably damaging	1.00
IGL03383:Wee1	APN	7	109,738,899 (GRCm39)	missense	probably damaging	1.00
R0220:Wee1	UTSW	7	109,723,733 (GRCm39)	missense	probably benign	0.10
R1934:Wee1	UTSW	7	109,721,698 (GRCm39)	missense	probably benign	0.06
R3110:Wee1	UTSW	7	109,730,043 (GRCm39)	missense	probably damaging	1.00
R3112:Wee1	UTSW	7	109,730,043 (GRCm39)	missense	probably damaging	1.00
R3978:Wee1	UTSW	7	109,723,762 (GRCm39)	missense	probably damaging	1.00
R4348:Wee1	UTSW	7	109,730,165 (GRCm39)	missense	probably damaging	1.00
R5434:Wee1	UTSW	7	109,723,776 (GRCm39)	frame shift	probably null
R5436:Wee1	UTSW	7	109,723,776 (GRCm39)	frame shift	probably null
R5449:Wee1	UTSW	7	109,723,776 (GRCm39)	frame shift	probably null
R5566:Wee1	UTSW	7	109,725,257 (GRCm39)	nonsense	probably null
R5630:Wee1	UTSW	7	109,723,776 (GRCm39)	frame shift	probably null
R5632:Wee1	UTSW	7	109,723,776 (GRCm39)	frame shift	probably null
R5685:Wee1	UTSW	7	109,723,776 (GRCm39)	frame shift	probably null
R5694:Wee1	UTSW	7	109,723,776 (GRCm39)	frame shift	probably null
R5807:Wee1	UTSW	7	109,723,776 (GRCm39)	frame shift	probably null
R5941:Wee1	UTSW	7	109,723,776 (GRCm39)	frame shift	probably null
R6044:Wee1	UTSW	7	109,738,513 (GRCm39)	missense	probably benign	0.00
R6163:Wee1	UTSW	7	109,734,858 (GRCm39)	missense	probably damaging	1.00
R6826:Wee1	UTSW	7	109,723,870 (GRCm39)	critical splice donor site	probably null
R7203:Wee1	UTSW	7	109,734,001 (GRCm39)	missense	probably benign	0.00
R7835:Wee1	UTSW	7	109,730,085 (GRCm39)	nonsense	probably null
R8273:Wee1	UTSW	7	109,723,691 (GRCm39)	missense	probably benign	0.00
R8953:Wee1	UTSW	7	109,723,691 (GRCm39)	missense	probably benign	0.00
R9077:Wee1	UTSW	7	109,725,963 (GRCm39)	missense	probably damaging	1.00
R9336:Wee1	UTSW	7	109,721,689 (GRCm39)	missense	probably damaging	1.00
R9463:Wee1	UTSW	7	109,721,917 (GRCm39)	missense	probably damaging	1.00
R9673:Wee1	UTSW	7	109,725,210 (GRCm39)	missense	probably damaging	0.98
R9748:Wee1	UTSW	7	109,721,722 (GRCm39)	nonsense	probably null

Predicted Primers

PCR Primer
(F):5'- TTGGCCACTTAGGAAGGCAAG -3'
(R):5'- GCCATTCAGTAGAAACCTAATTGTG -3'

Sequencing Primer
(F):5'- GTTGGAGGTTATATAGAGAAGACTCC -3'
(R):5'- AACCTAATTGTGTGCCAGGC -3'

Posted On

2016-09-01