Mutagenetix > Incidental Mutation

Incidental Mutation 'R5486:Taar7a'

430371

Institutional Source

Beutler Lab

Gene Symbol

Taar7a

Ensembl Gene

ENSMUSG00000095647

Gene Name

trace amine-associated receptor 7A

Synonyms

Taar7a, LOC215856

MMRRC Submission

043047-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.084) question?

Stock #

R5486 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

23868303-23869379 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to A at 23868356 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Threonine to Serine at position 342 (T342S)

Ref Sequence

ENSEMBL: ENSMUSP00000077616 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000078532]

AlphaFold

Q5QD12

Predicted Effect

probably benign
Transcript: ENSMUST00000078532
AA Change: T342S

PolyPhen 2 Score 0.001 (Sensitivity: 0.99; Specificity: 0.15)

SMART Domains

Protein: ENSMUSP00000077616
Gene: ENSMUSG00000095647
AA Change: T342S

Domain	Start	End	E-Value	Type
Pfam:7tm_4	54	344	9.2e-10	PFAM
Pfam:7TM_GPCR_Srx	56	261	5.2e-9	PFAM
Pfam:7tm_1	64	326	3.2e-58	PFAM

Coding Region Coverage

1x: 98.2%
3x: 97.2%
10x: 95.1%
20x: 90.4%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 43 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
A930009A15Rik	G	T	10: 115,415,810 (GRCm39)		probably benign	Het
Acad8	A	T	9: 26,910,791 (GRCm39)	M1K	probably null	Het
Adam12	C	A	7: 133,509,401 (GRCm39)	R786S	possibly damaging	Het
Add3	G	A	19: 53,232,818 (GRCm39)	V604I	probably benign	Het
Alpk2	A	T	18: 65,427,425 (GRCm39)		probably null	Het
Ano3	T	C	2: 110,576,215 (GRCm39)	D102G	probably damaging	Het
Bdp1	T	C	13: 100,235,018 (GRCm39)	Y192C	probably damaging	Het
Bod1l	A	T	5: 41,964,524 (GRCm39)	D2693E	possibly damaging	Het
Ccdc7a	T	C	8: 129,711,884 (GRCm39)	N284D	probably damaging	Het
Clic6	A	G	16: 92,326,740 (GRCm39)		probably null	Het
Cln5	T	C	14: 103,313,630 (GRCm39)	I294T	probably damaging	Het
Cmklr1	C	G	5: 113,752,990 (GRCm39)	D4H	possibly damaging	Het
Cyp2d9	T	A	15: 82,336,779 (GRCm39)	W43R	probably damaging	Het
Dnajb12	GC	G	10: 59,728,574 (GRCm39)		probably null	Het
Erlec1	A	T	11: 30,885,047 (GRCm39)	H413Q	probably damaging	Het
Fam168a	T	A	7: 100,483,376 (GRCm39)	M203K	probably damaging	Het
Fat2	A	T	11: 55,144,507 (GRCm39)	S4122R	probably benign	Het
Fgd4	A	T	16: 16,292,901 (GRCm39)	L272Q	probably damaging	Het
Hpcal4	A	G	4: 123,084,557 (GRCm39)	K162R	probably benign	Het
Iars1	T	A	13: 49,863,049 (GRCm39)		probably null	Het
Lbr	A	G	1: 181,646,403 (GRCm39)		probably null	Het
Lrp2	T	C	2: 69,267,809 (GRCm39)	I4259V	probably benign	Het
Mcm3	C	T	1: 20,885,118 (GRCm39)	G189S	probably damaging	Het
Nr1d2	A	G	14: 18,206,860 (GRCm38)	V137A	possibly damaging	Het
Or52d1	C	A	7: 103,755,705 (GRCm39)	T73N	probably damaging	Het
Or52n3	T	A	7: 104,530,168 (GRCm39)	C85S	probably benign	Het
Or7g33	A	G	9: 19,448,590 (GRCm39)	V212A	probably benign	Het
Pim3	T	C	15: 88,747,425 (GRCm39)	V97A	possibly damaging	Het
Piwil2	T	C	14: 70,638,880 (GRCm39)	N479S	probably benign	Het
Pld3	C	A	7: 27,233,156 (GRCm39)	W365L	probably damaging	Het
Plk3	C	A	4: 116,987,600 (GRCm39)	E412*	probably null	Het
Psmd1	A	G	1: 86,064,772 (GRCm39)	I935V	possibly damaging	Het
Sh2b2	A	G	5: 136,260,944 (GRCm39)	S91P	probably benign	Het
Skor2	A	G	18: 76,946,395 (GRCm39)	N39S	unknown	Het
Slc22a22	A	G	15: 57,126,847 (GRCm39)	V55A	probably damaging	Het
Smg7	A	G	1: 152,721,927 (GRCm39)	S595P	probably damaging	Het
Snrnp200	C	T	2: 127,074,986 (GRCm39)	P1520S	possibly damaging	Het
Tecpr2	A	T	12: 110,899,449 (GRCm39)	I606F	probably benign	Het
Tex19.2	A	T	11: 121,008,304 (GRCm39)	M48K	probably benign	Het
Thoc1	A	G	18: 9,992,204 (GRCm39)	T511A	probably benign	Het
Trpc2	GTGTCCTA	GTGTCCTATGTCCTA	7: 101,744,420 (GRCm39)		probably null	Het
Ubr5	C	A	15: 38,008,983 (GRCm39)	A1077S	probably benign	Het
Wdr95	A	T	5: 149,519,795 (GRCm39)	R571*	probably null	Het

Other mutations in Taar7a

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00476:Taar7a	APN	10	23,868,294 (GRCm39)	unclassified	probably benign
IGL02408:Taar7a	APN	10	23,868,500 (GRCm39)	missense	probably benign
PIT4434001:Taar7a	UTSW	10	23,869,319 (GRCm39)	missense	probably benign
R0422:Taar7a	UTSW	10	23,869,172 (GRCm39)	missense	probably benign	0.02
R1029:Taar7a	UTSW	10	23,868,439 (GRCm39)	missense	possibly damaging	0.65
R1742:Taar7a	UTSW	10	23,869,117 (GRCm39)	missense	probably damaging	0.98
R2104:Taar7a	UTSW	10	23,868,959 (GRCm39)	missense	probably damaging	0.99
R2421:Taar7a	UTSW	10	23,868,415 (GRCm39)	missense	probably damaging	1.00
R3907:Taar7a	UTSW	10	23,868,457 (GRCm39)	missense	probably benign	0.25
R4021:Taar7a	UTSW	10	23,869,284 (GRCm39)	missense	probably benign	0.00
R5180:Taar7a	UTSW	10	23,869,046 (GRCm39)	missense	probably damaging	1.00
R5507:Taar7a	UTSW	10	23,868,529 (GRCm39)	missense	probably damaging	0.98
R5587:Taar7a	UTSW	10	23,868,726 (GRCm39)	missense	probably benign	0.00
R5766:Taar7a	UTSW	10	23,869,260 (GRCm39)	missense	probably benign	0.01
R5861:Taar7a	UTSW	10	23,868,337 (GRCm39)	missense	probably benign
R7201:Taar7a	UTSW	10	23,868,358 (GRCm39)	missense	probably benign	0.04
R7506:Taar7a	UTSW	10	23,868,892 (GRCm39)	missense	possibly damaging	0.50
R8810:Taar7a	UTSW	10	23,869,279 (GRCm39)	missense	probably benign	0.20
R9015:Taar7a	UTSW	10	23,868,733 (GRCm39)	missense	probably damaging	0.97
R9172:Taar7a	UTSW	10	23,868,677 (GRCm39)	missense	probably benign	0.16
R9686:Taar7a	UTSW	10	23,869,309 (GRCm39)	missense	probably benign	0.01
R9717:Taar7a	UTSW	10	23,868,799 (GRCm39)	missense	probably benign	0.01
R9720:Taar7a	UTSW	10	23,868,733 (GRCm39)	missense	probably benign	0.10
X0064:Taar7a	UTSW	10	23,868,515 (GRCm39)	missense	probably damaging	1.00
Z1177:Taar7a	UTSW	10	23,868,790 (GRCm39)	missense	probably benign	0.01

Predicted Primers

PCR Primer
(F):5'- TCAGGCAGCATGAATAGCTG -3'
(R):5'- AAACCCTGGGCATTGCAGTG -3'

Sequencing Primer
(F):5'- GGATTTACATGCTTGGATCACAGCTC -3'
(R):5'- AGTGGCTGCCTTCCTCC -3'

Posted On

2016-10-05