Mutagenetix > Incidental Mutation

Incidental Mutation 'R5487:Vmn2r65'

430412

Institutional Source

Beutler Lab

Gene Symbol

Vmn2r65

Ensembl Gene

ENSMUSG00000066372

Gene Name

vomeronasal 2, receptor 65

Synonyms

ENSMUSG00000070600

MMRRC Submission

043048-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.073) question?

Stock #

R5487 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

84589377-84613217 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

G to T at 84595529 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Proline to Glutamine at position 385 (P385Q)

Ref Sequence

ENSEMBL: ENSMUSP00000036551 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000044583]

AlphaFold

G3X931

Predicted Effect

possibly damaging
Transcript: ENSMUST00000044583
AA Change: P385Q

PolyPhen 2 Score 0.758 (Sensitivity: 0.85; Specificity: 0.92)

SMART Domains

Protein: ENSMUSP00000036551
Gene: ENSMUSG00000066372
AA Change: P385Q

Domain	Start	End	E-Value	Type
signal peptide	1	20	N/A	INTRINSIC
Pfam:ANF_receptor	73	435	5.6e-41	PFAM
Pfam:NCD3G	501	553	1.3e-21	PFAM
Pfam:7tm_3	584	821	2.3e-53	PFAM

Coding Region Coverage

1x: 98.2%
3x: 97.1%
10x: 94.6%
20x: 89.0%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 41 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Abca8b	A	G	11: 109,844,340 (GRCm39)	V959A	probably damaging	Het
Alg3	A	T	16: 20,426,530 (GRCm39)	I115N	probably damaging	Het
Ash1l	A	G	3: 88,888,733 (GRCm39)	D204G	probably benign	Het
Camta1	C	A	4: 151,229,211 (GRCm39)	E540D	possibly damaging	Het
Cblc	T	C	7: 19,518,733 (GRCm39)	T413A	probably benign	Het
Ccdc122	T	A	14: 77,329,119 (GRCm39)	H57Q	probably benign	Het
Ccdc85a	A	T	11: 28,526,768 (GRCm39)	L280*	probably null	Het
Cd46	T	G	1: 194,750,478 (GRCm39)		probably null	Het
Cmklr1	C	G	5: 113,752,990 (GRCm39)	D4H	possibly damaging	Het
Dnaaf3	T	C	7: 4,526,864 (GRCm39)		probably null	Het
Dnajb12	GC	G	10: 59,728,574 (GRCm39)		probably null	Het
Ecpas	T	C	4: 58,809,421 (GRCm39)	D1651G	probably benign	Het
Eif2ak1	A	G	5: 143,833,981 (GRCm39)		probably null	Het
Erich4	A	G	7: 25,314,664 (GRCm39)	M83T	probably benign	Het
Fbxo24	C	T	5: 137,617,094 (GRCm39)	G331E	possibly damaging	Het
Fzd4	A	G	7: 89,056,615 (GRCm39)	I221V	probably benign	Het
G6pc2	T	C	2: 69,056,921 (GRCm39)	V189A	probably damaging	Het
Gm10663	A	C	8: 65,527,686 (GRCm39)	E1A	probably null	Het
Gm4871	C	A	5: 144,967,199 (GRCm39)	E178D	probably damaging	Het
Igdcc3	A	G	9: 65,088,866 (GRCm39)	E415G	probably damaging	Het
Kmt2a	A	T	9: 44,733,272 (GRCm39)		probably benign	Het
Krba1	T	C	6: 48,380,973 (GRCm39)	V103A	probably damaging	Het
Lcat	T	C	8: 106,666,296 (GRCm39)	K409E	probably benign	Het
Lrriq4	C	A	3: 30,714,144 (GRCm39)	N497K	probably benign	Het
Mical2	A	G	7: 111,919,842 (GRCm39)	T451A	probably damaging	Het
Noxo1	C	T	17: 24,917,291 (GRCm39)		probably benign	Het
Oas1a	A	G	5: 121,045,490 (GRCm39)	I17T	probably damaging	Het
Otog	A	T	7: 45,938,192 (GRCm39)	Y1967F	probably benign	Het
Pcdha9	A	T	18: 37,132,703 (GRCm39)	N591Y	probably damaging	Het
Plxna4	A	G	6: 32,494,218 (GRCm39)	Y133H	probably damaging	Het
Pou5f2	T	C	13: 78,173,118 (GRCm39)	L20P	probably benign	Het
Prkcb	T	A	7: 122,199,948 (GRCm39)	C586*	probably null	Het
Sema3b	A	T	9: 107,478,161 (GRCm39)	M408K	probably damaging	Het
Serpinf2	G	A	11: 75,324,031 (GRCm39)	T332I	probably damaging	Het
Tmbim1	A	G	1: 74,332,164 (GRCm39)	V121A	probably benign	Het
Tph2	C	T	10: 114,955,779 (GRCm39)	G338D	probably damaging	Het
Trpc2	GTGTCCTA	GTGTCCTATGTCCTA	7: 101,744,420 (GRCm39)		probably null	Het
Ttn	C	T	2: 76,642,896 (GRCm39)	V13247M	probably damaging	Het
Uty	C	T	Y: 1,174,825 (GRCm39)	G192R	probably damaging	Het
Wdfy3	G	A	5: 101,984,140 (GRCm39)	R3489W	probably damaging	Het
Zfp750	A	G	11: 121,404,558 (GRCm39)	S106P	probably benign	Het

Other mutations in Vmn2r65

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00942:Vmn2r65	APN	7	84,592,761 (GRCm39)	missense	probably damaging	0.99
IGL01104:Vmn2r65	APN	7	84,589,996 (GRCm39)	missense	possibly damaging	0.93
IGL01154:Vmn2r65	APN	7	84,592,729 (GRCm39)	missense	probably benign	0.00
IGL01453:Vmn2r65	APN	7	84,589,708 (GRCm39)	missense	probably damaging	1.00
IGL01675:Vmn2r65	APN	7	84,596,587 (GRCm39)	missense	probably benign	0.00
IGL01748:Vmn2r65	APN	7	84,589,507 (GRCm39)	missense	probably damaging	1.00
IGL01997:Vmn2r65	APN	7	84,589,978 (GRCm39)	missense	probably damaging	1.00
IGL02527:Vmn2r65	APN	7	84,595,724 (GRCm39)	missense	possibly damaging	0.82
IGL02617:Vmn2r65	APN	7	84,589,549 (GRCm39)	missense	probably damaging	1.00
IGL02676:Vmn2r65	APN	7	84,589,381 (GRCm39)	missense	possibly damaging	0.86
IGL03084:Vmn2r65	APN	7	84,592,354 (GRCm39)	missense	probably damaging	1.00
A4554:Vmn2r65	UTSW	7	84,595,791 (GRCm39)	missense	probably damaging	0.96
PIT4651001:Vmn2r65	UTSW	7	84,595,461 (GRCm39)	missense	probably benign	0.12
R0322:Vmn2r65	UTSW	7	84,595,756 (GRCm39)	missense	probably benign	0.01
R0453:Vmn2r65	UTSW	7	84,595,442 (GRCm39)	missense	probably benign	0.03
R0862:Vmn2r65	UTSW	7	84,592,853 (GRCm39)	missense	probably benign	0.00
R1392:Vmn2r65	UTSW	7	84,596,624 (GRCm39)	missense	probably benign	0.14
R1392:Vmn2r65	UTSW	7	84,596,624 (GRCm39)	missense	probably benign	0.14
R1508:Vmn2r65	UTSW	7	84,589,886 (GRCm39)	missense	probably benign	0.00
R1687:Vmn2r65	UTSW	7	84,590,026 (GRCm39)	missense	probably benign	0.02
R1876:Vmn2r65	UTSW	7	84,595,505 (GRCm39)	missense	probably damaging	1.00
R2136:Vmn2r65	UTSW	7	84,592,781 (GRCm39)	missense	probably damaging	0.96
R2259:Vmn2r65	UTSW	7	84,590,119 (GRCm39)	missense	possibly damaging	0.86
R2338:Vmn2r65	UTSW	7	84,590,051 (GRCm39)	missense	possibly damaging	0.86
R2880:Vmn2r65	UTSW	7	84,613,094 (GRCm39)	missense	probably damaging	1.00
R3410:Vmn2r65	UTSW	7	84,595,896 (GRCm39)	missense	probably benign	0.00
R3411:Vmn2r65	UTSW	7	84,595,896 (GRCm39)	missense	probably benign	0.00
R3770:Vmn2r65	UTSW	7	84,589,623 (GRCm39)	missense	probably damaging	1.00
R3800:Vmn2r65	UTSW	7	84,589,738 (GRCm39)	missense	possibly damaging	0.93
R3850:Vmn2r65	UTSW	7	84,595,859 (GRCm39)	missense	probably benign	0.00
R4105:Vmn2r65	UTSW	7	84,595,691 (GRCm39)	missense	probably benign	0.03
R4568:Vmn2r65	UTSW	7	84,596,677 (GRCm39)	missense	probably damaging	1.00
R4677:Vmn2r65	UTSW	7	84,613,082 (GRCm39)	missense	possibly damaging	0.55
R4768:Vmn2r65	UTSW	7	84,596,602 (GRCm39)	missense	probably damaging	1.00
R4778:Vmn2r65	UTSW	7	84,592,801 (GRCm39)	missense	possibly damaging	0.47
R5057:Vmn2r65	UTSW	7	84,589,819 (GRCm39)	missense	probably damaging	1.00
R5279:Vmn2r65	UTSW	7	84,589,849 (GRCm39)	missense	probably damaging	1.00
R5280:Vmn2r65	UTSW	7	84,595,542 (GRCm39)	missense	probably damaging	0.99
R5394:Vmn2r65	UTSW	7	84,595,862 (GRCm39)	missense	probably benign	0.04
R5580:Vmn2r65	UTSW	7	84,596,802 (GRCm39)	missense	probably damaging	0.99
R5638:Vmn2r65	UTSW	7	84,590,047 (GRCm39)	missense	probably damaging	1.00
R5673:Vmn2r65	UTSW	7	84,596,615 (GRCm39)	missense	probably benign	0.23
R5688:Vmn2r65	UTSW	7	84,589,900 (GRCm39)	missense	probably benign	0.00
R5935:Vmn2r65	UTSW	7	84,592,869 (GRCm39)	missense	probably benign	0.00
R6354:Vmn2r65	UTSW	7	84,589,574 (GRCm39)	missense	probably benign	0.35
R6372:Vmn2r65	UTSW	7	84,589,861 (GRCm39)	missense	probably damaging	1.00
R6924:Vmn2r65	UTSW	7	84,613,198 (GRCm39)	missense	probably benign	0.20
R7021:Vmn2r65	UTSW	7	84,596,587 (GRCm39)	missense	probably benign	0.00
R7195:Vmn2r65	UTSW	7	84,592,347 (GRCm39)	critical splice donor site	probably null
R7422:Vmn2r65	UTSW	7	84,595,569 (GRCm39)	missense	probably damaging	0.99
R7654:Vmn2r65	UTSW	7	84,590,261 (GRCm39)	missense	probably damaging	1.00
R7686:Vmn2r65	UTSW	7	84,589,744 (GRCm39)	missense	probably damaging	0.99
R7691:Vmn2r65	UTSW	7	84,592,851 (GRCm39)	missense	probably benign	0.30
R7798:Vmn2r65	UTSW	7	84,596,192 (GRCm39)	missense	probably damaging	1.00
R7798:Vmn2r65	UTSW	7	84,595,530 (GRCm39)	missense	probably benign	0.00
R8103:Vmn2r65	UTSW	7	84,595,919 (GRCm39)	missense	probably damaging	1.00
R8272:Vmn2r65	UTSW	7	84,596,817 (GRCm39)	missense	probably benign	0.02
R8303:Vmn2r65	UTSW	7	84,589,391 (GRCm39)	nonsense	probably null
R8354:Vmn2r65	UTSW	7	84,589,402 (GRCm39)	missense	possibly damaging	0.73
R8454:Vmn2r65	UTSW	7	84,589,402 (GRCm39)	missense	possibly damaging	0.73
R8489:Vmn2r65	UTSW	7	84,589,964 (GRCm39)	missense	possibly damaging	0.85
R8554:Vmn2r65	UTSW	7	84,595,960 (GRCm39)	missense	probably benign	0.00
R8680:Vmn2r65	UTSW	7	84,589,388 (GRCm39)	missense	probably benign	0.00
R8731:Vmn2r65	UTSW	7	84,589,447 (GRCm39)	nonsense	probably null
R8839:Vmn2r65	UTSW	7	84,595,489 (GRCm39)	nonsense	probably null
R8847:Vmn2r65	UTSW	7	84,590,212 (GRCm39)	missense	probably damaging	1.00
R8916:Vmn2r65	UTSW	7	84,595,665 (GRCm39)	missense	probably benign	0.21
R9254:Vmn2r65	UTSW	7	84,596,650 (GRCm39)	missense	probably damaging	0.99
R9379:Vmn2r65	UTSW	7	84,596,650 (GRCm39)	missense	probably damaging	0.99
R9392:Vmn2r65	UTSW	7	84,589,718 (GRCm39)	missense	possibly damaging	0.95
R9563:Vmn2r65	UTSW	7	84,589,880 (GRCm39)	nonsense	probably null
R9686:Vmn2r65	UTSW	7	84,590,084 (GRCm39)	missense	probably benign	0.45
X0067:Vmn2r65	UTSW	7	84,590,113 (GRCm39)	missense	probably benign	0.04
Z1088:Vmn2r65	UTSW	7	84,592,473 (GRCm39)	critical splice acceptor site	probably null
Z1177:Vmn2r65	UTSW	7	84,590,234 (GRCm39)	missense	probably damaging	0.97

Predicted Primers

PCR Primer
(F):5'- CACCAGTAACCAGGTTCATGTAATATG -3'
(R):5'- TTCATCCAGACAGTGCACC -3'

Sequencing Primer
(F):5'- GCTACAGAAATAATTCCACTTGTGAG -3'
(R):5'- GTGCACCCTTCCAACTACAGTAAG -3'

Posted On

2016-10-05