|Institutional Source||Beutler Lab|
|Gene Name||G protein-coupled receptor kinase 4|
|Is this an essential gene?||Probably non essential (E-score: 0.169)|
|Stock #||R0469 (G1)|
|Chromosomal Location||34660379-34755305 bp(+) (GRCm38)|
|Type of Mutation||missense|
|DNA Base Change (assembly)||A to G at 34716213 bp|
|Amino Acid Change||Threonine to Alanine at position 208 (T208A)|
|Ref Sequence||ENSEMBL: ENSMUSP00000001112 (fasta)|
|Gene Model||predicted gene model for transcript(s): [ENSMUST00000001112]|
|Predicted Effect||probably damaging
AA Change: T208A
PolyPhen 2 Score 0.972 (Sensitivity: 0.77; Specificity: 0.96)
AA Change: T208A
|Predicted Effect||noncoding transcript
|Meta Mutation Damage Score||0.32|
|Coding Region Coverage||
FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes a member of the guanine nucleotide-binding protein (G protein)-coupled receptor kinase subfamily of the Ser/Thr protein kinase family. The protein phosphorylates the activated forms of G protein-coupled receptors thus initiating its deactivation. This gene has been linked to both genetic and acquired hypertension. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2013]
PHENOTYPE: Mice heterozygous for a knock-out allele are viable, fertile and overtly normal. [provided by MGI curators]
|Allele List at MGI|
|Other mutations in this stock||
|Other mutations in Grk4||
(F):5'- ACATTCACTTCATTCCCAGCAGCTAC -3'
(R):5'- TCCAATAGGGGACAGCAAAACTCCA -3'
(F):5'- CGTCTGAGGAAGATCCAGC -3'
(R):5'- tgaacagcagaggtgacag -3'