Mutagenetix > Incidental Mutation

Incidental Mutation 'R5526:Or14j7'

431886

Institutional Source

Beutler Lab

Gene Symbol

Or14j7

Ensembl Gene

ENSMUSG00000059030

Gene Name

olfactory receptor family 14 subfamily J member 7

Synonyms

MOR218-13, GA_x6K02T2PSCP-2374126-2375048, Olfr128

MMRRC Submission

043084-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.069) question?

Stock #

R5526 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

38234459-38235385 bp(+) (GRCm39)

Type of Mutation

makesense

DNA Base Change (assembly)

T to A at 38235383 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Stop codon to Lysine at position 309 (*309K)

Ref Sequence

ENSEMBL: ENSMUSP00000151065 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000080231] [ENSMUST00000216440]

AlphaFold

Q7TRJ1

Predicted Effect

probably null
Transcript: ENSMUST00000080231
AA Change: *309K

SMART Domains

Protein: ENSMUSP00000079121
Gene: ENSMUSG00000059030
AA Change: *309K

Domain	Start	End	E-Value	Type
Pfam:7tm_4	31	308	1.3e-46	PFAM
Pfam:7TM_GPCR_Srsx	35	305	1.1e-5	PFAM
Pfam:7tm_1	41	290	1.6e-21	PFAM

Predicted Effect

probably null
Transcript: ENSMUST00000216440
AA Change: *309K

Coding Region Coverage

1x: 98.3%
3x: 97.3%
10x: 95.2%
20x: 90.7%

Validation Efficiency

MGI Phenotype

FUNCTION: Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]

Allele List at MGI

Other mutations in this stock

Total: 55 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Abca12	A	T	1: 71,331,605 (GRCm39)	M1315K	probably benign	Het
Abcc4	C	T	14: 118,868,449 (GRCm39)	V168I	probably benign	Het
Add3	T	A	19: 53,215,038 (GRCm39)	L71Q	probably damaging	Het
Agbl5	G	A	5: 31,051,247 (GRCm39)		probably null	Het
Angpt1	A	G	15: 42,375,737 (GRCm39)	L173P	probably damaging	Het
Crls1	T	A	2: 132,703,165 (GRCm39)	M205K	possibly damaging	Het
Cspg4b	T	C	13: 113,504,427 (GRCm39)	V1852A	probably benign	Het
Dhtkd1	A	T	2: 5,916,662 (GRCm39)	N671K	probably damaging	Het
Dmbt1	A	T	7: 130,642,920 (GRCm39)	D246V	probably damaging	Het
Dolk	G	A	2: 30,175,820 (GRCm39)	A75V	probably damaging	Het
Dtna	T	A	18: 23,779,287 (GRCm39)	V623E	probably damaging	Het
Elavl3	T	C	9: 21,947,622 (GRCm39)	T106A	probably benign	Het
Ergic1	T	C	17: 26,843,652 (GRCm39)	C41R	probably damaging	Het
Fat2	T	A	11: 55,160,187 (GRCm39)	I3309F	possibly damaging	Het
Fbn1	C	A	2: 125,207,559 (GRCm39)	R978L	possibly damaging	Het
Fzd6	A	T	15: 38,894,559 (GRCm39)	S242C	possibly damaging	Het
Galntl6	T	A	8: 58,926,004 (GRCm39)	H87L	probably benign	Het
Gatad2a	T	C	8: 70,388,591 (GRCm39)	E32G	probably damaging	Het
Gemin6	T	G	17: 80,535,178 (GRCm39)	V46G	probably damaging	Het
Gm11541	G	T	11: 94,594,944 (GRCm39)	H41Q	unknown	Het
Has3	A	G	8: 107,600,579 (GRCm39)	T14A	probably damaging	Het
Kmt2b	A	G	7: 30,279,869 (GRCm39)	L1377P	probably damaging	Het
Lcorl	T	C	5: 45,891,069 (GRCm39)	N428S	probably benign	Het
Lipo5	A	T	19: 33,445,284 (GRCm39)	V95D	unknown	Het
Lrp1	A	T	10: 127,391,593 (GRCm39)	V2942D	probably benign	Het
Map1a	T	C	2: 121,136,143 (GRCm39)	S2082P	probably damaging	Het
Mast4	T	C	13: 102,890,723 (GRCm39)	S852G	possibly damaging	Het
Mlh3	A	T	12: 85,316,147 (GRCm39)	L13*	probably null	Het
Ncam2	C	T	16: 81,231,766 (GRCm39)	R77*	probably null	Het
Nlrp9c	T	A	7: 26,081,791 (GRCm39)	N645I	possibly damaging	Het
Or12d17	T	A	17: 37,778,003 (GRCm39)	L302Q	unknown	Het
Or12e7	T	C	2: 87,288,109 (GRCm39)	I200T	probably benign	Het
Or7e166	C	A	9: 19,624,994 (GRCm39)	Y290*	probably null	Het
Or8g52	A	T	9: 39,630,892 (GRCm39)	Y123F	possibly damaging	Het
Pex12	A	G	11: 83,187,090 (GRCm39)	V286A	possibly damaging	Het
Phax	C	T	18: 56,717,382 (GRCm39)	T275I	probably damaging	Het
Phf21b	A	G	15: 84,676,006 (GRCm39)	V335A	probably benign	Het
Psg18	A	T	7: 18,083,273 (GRCm39)	L173H	probably damaging	Het
Rab3ip	T	C	10: 116,754,834 (GRCm39)	T209A	possibly damaging	Het
Ralgapb	T	C	2: 158,274,705 (GRCm39)	V202A	probably damaging	Het
Rpn2	T	C	2: 157,165,187 (GRCm39)	L611P	probably damaging	Het
Runx2	T	G	17: 45,035,749 (GRCm39)	T148P	probably damaging	Het
Sbpl	C	A	17: 24,173,623 (GRCm39)	D50Y	probably damaging	Het
Scn5a	G	A	9: 119,350,237 (GRCm39)	P879L	probably damaging	Het
Sfn	T	C	4: 133,328,915 (GRCm39)	R56G	probably damaging	Het
Spata31g1	T	C	4: 42,972,125 (GRCm39)	V486A	possibly damaging	Het
Tas2r138	C	T	6: 40,589,914 (GRCm39)	A111T	probably benign	Het
Tasp1	A	T	2: 139,850,709 (GRCm39)	S105T	probably damaging	Het
Tmem225	T	G	9: 40,062,002 (GRCm39)	H205Q	possibly damaging	Het
Tmprss7	A	G	16: 45,481,267 (GRCm39)	S640P	probably damaging	Het
Utp4	G	A	8: 107,644,265 (GRCm39)	A535T	possibly damaging	Het
Vmn1r203	A	T	13: 22,708,273 (GRCm39)	D18V	probably benign	Het
Vmn2r58	A	G	7: 41,522,069 (GRCm39)	L9P	probably benign	Het
Zcchc2	T	A	1: 105,957,984 (GRCm39)	C420*	probably null	Het
Zfp804a	A	G	2: 82,088,934 (GRCm39)	D921G	probably benign	Het

Other mutations in Or14j7

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01922:Or14j7	APN	17	38,234,850 (GRCm39)	missense	possibly damaging	0.73
IGL01940:Or14j7	APN	17	38,235,177 (GRCm39)	missense	probably damaging	1.00
R0427:Or14j7	UTSW	17	38,234,520 (GRCm39)	missense	probably benign	0.00
R0462:Or14j7	UTSW	17	38,234,667 (GRCm39)	missense	probably damaging	1.00
R0546:Or14j7	UTSW	17	38,235,229 (GRCm39)	nonsense	probably null
R0760:Or14j7	UTSW	17	38,235,005 (GRCm39)	nonsense	probably null
R0940:Or14j7	UTSW	17	38,234,591 (GRCm39)	missense	probably damaging	0.98
R1898:Or14j7	UTSW	17	38,234,516 (GRCm39)	missense	possibly damaging	0.91
R1935:Or14j7	UTSW	17	38,234,993 (GRCm39)	missense	probably damaging	1.00
R2060:Or14j7	UTSW	17	38,234,771 (GRCm39)	missense	probably benign	0.00
R4680:Or14j7	UTSW	17	38,234,813 (GRCm39)	missense	probably damaging	1.00
R5165:Or14j7	UTSW	17	38,235,252 (GRCm39)	missense	probably benign	0.14
R5594:Or14j7	UTSW	17	38,234,502 (GRCm39)	missense	probably benign	0.00
R5751:Or14j7	UTSW	17	38,234,861 (GRCm39)	missense	probably benign	0.44
R6330:Or14j7	UTSW	17	38,234,685 (GRCm39)	missense	possibly damaging	0.77
R7123:Or14j7	UTSW	17	38,234,567 (GRCm39)	missense	probably benign	0.33
R7354:Or14j7	UTSW	17	38,235,284 (GRCm39)	missense	probably benign	0.00
R7827:Or14j7	UTSW	17	38,234,568 (GRCm39)	nonsense	probably null
R8258:Or14j7	UTSW	17	38,234,847 (GRCm39)	missense	probably damaging	1.00
R8259:Or14j7	UTSW	17	38,234,847 (GRCm39)	missense	probably damaging	1.00
R8686:Or14j7	UTSW	17	38,235,168 (GRCm39)	missense	probably benign	0.00
R8787:Or14j7	UTSW	17	38,235,075 (GRCm39)	missense	probably benign	0.06
R9036:Or14j7	UTSW	17	38,235,168 (GRCm39)	missense	probably benign	0.03
R9278:Or14j7	UTSW	17	38,235,275 (GRCm39)	missense	probably benign
R9761:Or14j7	UTSW	17	38,234,934 (GRCm39)	missense	probably benign	0.03
Z1177:Or14j7	UTSW	17	38,234,618 (GRCm39)	missense	probably benign	0.02

Predicted Primers

PCR Primer
(F):5'- CTGAAGGCCGATCTAAGGTC -3'
(R):5'- GGTTCAAAGAATCACATCACAACTTGC -3'

Sequencing Primer
(F):5'- AGGCCGATCTAAGGTCTTCTC -3'
(R):5'- GAATCACATCACAACTTGCTATGC -3'

Posted On

2016-10-05