Mutagenetix > Incidental Mutation

Incidental Mutation 'R0478:Zfp760'

43221

Institutional Source

Beutler Lab

Gene Symbol

Zfp760

Ensembl Gene

ENSMUSG00000067928

Gene Name

zinc finger protein 760

Synonyms

MMRRC Submission

038678-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.130) question?

Stock #

R0478 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

21926723-21944617 bp(+) (GRCm39)

Type of Mutation

nonsense

DNA Base Change (assembly)

G to T at 21940995 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Glutamic Acid to Stop codon at position 57 (E57*)

Ref Sequence

ENSEMBL: ENSMUSP00000073038 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000073312]

AlphaFold

E9QAF5

Predicted Effect

probably null
Transcript: ENSMUST00000073312
AA Change: E57*

SMART Domains

Protein: ENSMUSP00000073038
Gene: ENSMUSG00000067928
AA Change: E57*

Domain	Start	End	E-Value	Type
KRAB	8	69	3.62e-21	SMART
ZnF_C2H2	183	202	1.78e2	SMART
ZnF_C2H2	208	230	1.69e-3	SMART
ZnF_C2H2	236	258	4.54e-4	SMART
ZnF_C2H2	264	286	2.79e-4	SMART
ZnF_C2H2	292	314	1.1e-2	SMART
ZnF_C2H2	320	342	2.71e-2	SMART
ZnF_C2H2	348	370	7.9e-4	SMART
ZnF_C2H2	376	398	1.72e-4	SMART
ZnF_C2H2	404	426	1.14e0	SMART
ZnF_C2H2	432	454	4.05e-1	SMART
ZnF_C2H2	460	482	5.14e-3	SMART
ZnF_C2H2	488	510	1.18e-2	SMART
ZnF_C2H2	516	538	1.95e-3	SMART
ZnF_C2H2	544	566	1.82e-3	SMART
ZnF_C2H2	572	594	4.17e-3	SMART
ZnF_C2H2	600	622	1.04e-3	SMART
ZnF_C2H2	628	650	1.28e-3	SMART

Meta Mutation Damage Score

0.9185

Coding Region Coverage

1x: 99.7%
3x: 99.0%
10x: 97.2%
20x: 94.9%

Validation Efficiency

100% (49/49)

Allele List at MGI

Other mutations in this stock

Total: 46 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
4930432E11Rik	A	T	7: 29,262,014 (GRCm39)		noncoding transcript	Het
4930556J24Rik	T	G	11: 3,926,259 (GRCm39)		probably benign	Het
Acnat1	T	G	4: 49,450,901 (GRCm39)	D70A	probably damaging	Het
Adnp2	A	G	18: 80,172,549 (GRCm39)	V620A	probably benign	Het
Aldoart1	T	A	4: 72,770,580 (GRCm39)	H21L	probably benign	Het
Birc2	A	G	9: 7,860,348 (GRCm39)	V290A	probably damaging	Het
Bpifb3	C	T	2: 153,773,400 (GRCm39)		probably benign	Het
Camta1	C	A	4: 151,159,597 (GRCm39)	R1614L	probably damaging	Het
Clmn	A	G	12: 104,751,750 (GRCm39)	M235T	probably damaging	Het
Dmbt1	A	G	7: 130,642,917 (GRCm39)	E245G	possibly damaging	Het
Epgn	G	T	5: 91,178,987 (GRCm39)	V36L	probably benign	Het
Ets2	C	A	16: 95,517,306 (GRCm39)	P346Q	probably damaging	Het
Fam222b	T	C	11: 78,044,682 (GRCm39)	L81P	probably damaging	Het
Fancf	A	C	7: 51,511,440 (GRCm39)	L188R	probably damaging	Het
Fibin	T	C	2: 110,193,079 (GRCm39)	D21G	possibly damaging	Het
Fzd6	A	G	15: 38,897,429 (GRCm39)		probably null	Het
Gbp4	T	A	5: 105,267,299 (GRCm39)	Q540L	probably benign	Het
Greb1l	T	A	18: 10,509,281 (GRCm39)	L531Q	probably damaging	Het
Il5ra	A	G	6: 106,715,423 (GRCm39)	V137A	probably benign	Het
Kif26a	G	A	12: 112,142,223 (GRCm39)	A826T	probably damaging	Het
Kiz	T	C	2: 146,784,078 (GRCm39)	V537A	possibly damaging	Het
Klhl32	C	T	4: 24,792,777 (GRCm39)	G15D	probably damaging	Het
Kmt2d	G	A	15: 98,751,462 (GRCm39)		probably benign	Het
Lbp	T	C	2: 158,159,448 (GRCm39)		probably benign	Het
Mmp25	T	C	17: 23,851,756 (GRCm39)	T318A	probably benign	Het
Mrpl50	A	G	4: 49,514,513 (GRCm39)	C53R	probably damaging	Het
Msl3l2	G	C	10: 55,991,411 (GRCm39)	E45D	probably damaging	Het
Nfxl1	A	G	5: 72,681,988 (GRCm39)		probably null	Het
Noc3l	A	G	19: 38,798,450 (GRCm39)		probably null	Het
Or12e7	T	A	2: 87,288,370 (GRCm39)	V287E	probably damaging	Het
Or1x6	T	C	11: 50,939,539 (GRCm39)	S202P	probably benign	Het
Pgm5	A	T	19: 24,812,233 (GRCm39)	S100T	possibly damaging	Het
Pi4ka	C	T	16: 17,127,175 (GRCm39)	G1093S	possibly damaging	Het
Pitrm1	T	A	13: 6,609,431 (GRCm39)	S350T	probably damaging	Het
Ptk2b	G	T	14: 66,450,821 (GRCm39)	N48K	probably damaging	Het
Septin3	T	C	15: 82,175,007 (GRCm39)	L172P	probably damaging	Het
Sirt3	A	T	7: 140,458,027 (GRCm39)	C41S		Het
Sphkap	C	T	1: 83,256,432 (GRCm39)	R152H	probably damaging	Het
St3gal1	T	C	15: 66,985,579 (GRCm39)	Y25C	probably damaging	Het
Tbc1d31	T	C	15: 57,795,932 (GRCm39)	F175S	probably damaging	Het
Tfdp2	T	A	9: 96,172,636 (GRCm39)	D43E	probably benign	Het
Tgm1	G	A	14: 55,937,791 (GRCm39)	Q773*	probably null	Het
Tmc3	A	T	7: 83,271,360 (GRCm39)	R837S	possibly damaging	Het
Unc13a	A	G	8: 72,103,792 (GRCm39)	V880A	possibly damaging	Het
Vmn1r237	T	A	17: 21,535,081 (GRCm39)	V268E	probably damaging	Het
Zan	C	T	5: 137,398,788 (GRCm39)		probably benign	Het

Other mutations in Zfp760

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00472:Zfp760	APN	17	21,942,457 (GRCm39)	missense	possibly damaging	0.75
IGL00862:Zfp760	APN	17	21,941,265 (GRCm39)	missense	probably benign	0.00
IGL02198:Zfp760	APN	17	21,941,193 (GRCm39)	missense	probably benign	0.00
R0835:Zfp760	UTSW	17	21,942,559 (GRCm39)	missense	possibly damaging	0.63
R1191:Zfp760	UTSW	17	21,942,286 (GRCm39)	missense	probably damaging	1.00
R1760:Zfp760	UTSW	17	21,941,311 (GRCm39)	missense	probably damaging	1.00
R2698:Zfp760	UTSW	17	21,939,935 (GRCm39)	missense	probably damaging	0.99
R3722:Zfp760	UTSW	17	21,941,143 (GRCm39)	missense	probably damaging	1.00
R4561:Zfp760	UTSW	17	21,942,648 (GRCm39)	missense	probably benign	0.00
R4700:Zfp760	UTSW	17	21,941,388 (GRCm39)	missense	probably benign	0.01
R4859:Zfp760	UTSW	17	21,942,516 (GRCm39)	nonsense	probably null
R4859:Zfp760	UTSW	17	21,942,511 (GRCm39)	missense	probably damaging	0.97
R4897:Zfp760	UTSW	17	21,942,229 (GRCm39)	missense	probably benign	0.02
R6675:Zfp760	UTSW	17	21,941,991 (GRCm39)	missense	possibly damaging	0.92
R7286:Zfp760	UTSW	17	21,941,760 (GRCm39)	missense	probably benign	0.04
R7336:Zfp760	UTSW	17	21,942,814 (GRCm39)	missense	unknown
R7356:Zfp760	UTSW	17	21,941,601 (GRCm39)	missense	probably benign
R7369:Zfp760	UTSW	17	21,942,214 (GRCm39)	missense	probably benign	0.00
R7504:Zfp760	UTSW	17	21,941,655 (GRCm39)	missense	probably damaging	0.97
R7553:Zfp760	UTSW	17	21,941,872 (GRCm39)	missense	possibly damaging	0.82
R7577:Zfp760	UTSW	17	21,941,242 (GRCm39)	nonsense	probably null
R7579:Zfp760	UTSW	17	21,941,907 (GRCm39)	missense	possibly damaging	0.93
R7608:Zfp760	UTSW	17	21,941,797 (GRCm39)	missense	probably benign	0.00
R7973:Zfp760	UTSW	17	21,941,084 (GRCm39)	missense	probably benign	0.00
R8078:Zfp760	UTSW	17	21,942,436 (GRCm39)	missense	probably benign	0.27
R8332:Zfp760	UTSW	17	21,942,301 (GRCm39)	missense	probably damaging	0.99
R8750:Zfp760	UTSW	17	21,941,356 (GRCm39)	missense	possibly damaging	0.56
R9094:Zfp760	UTSW	17	21,941,932 (GRCm39)	missense	possibly damaging	0.86
R9264:Zfp760	UTSW	17	21,942,663 (GRCm39)	missense	possibly damaging	0.80
R9372:Zfp760	UTSW	17	21,941,035 (GRCm39)	missense	probably benign	0.00
R9520:Zfp760	UTSW	17	21,941,036 (GRCm39)	missense	probably benign	0.00
R9564:Zfp760	UTSW	17	21,942,272 (GRCm39)	missense	possibly damaging	0.94
R9743:Zfp760	UTSW	17	21,942,338 (GRCm39)	missense	probably benign
X0057:Zfp760	UTSW	17	21,942,663 (GRCm39)	missense	possibly damaging	0.80

Predicted Primers

PCR Primer
(F):5'- CATCAATCCCCACAGTGTGATGGAG -3'
(R):5'- TGATTCAATGGAAGCATCCTGGTAGC -3'

Sequencing Primer
(F):5'- TGGAGGTTCTGAACATCCATAC -3'
(R):5'- AAGCATCCTGGTAGCTGTACTTG -3'

Posted On

2013-05-23