|Institutional Source||Beutler Lab|
|Gene Name||beta-carotene oxygenase 2|
|Synonyms||CMO2, Bcdo2, beta-diox-II, B-diox-II, Bcmo2|
|Is this an essential gene?||Probably non essential (E-score: 0.066)|
|Stock #||R5458 (G1)|
|Chromosomal Location||50533087-50555181 bp(-) (GRCm38)|
|Type of Mutation||splice site (3 bp from exon)|
|DNA Base Change (assembly)||T to C at 50545344 bp|
|Amino Acid Change|
|Ref Sequence||ENSEMBL: ENSMUSP00000112727 (fasta)|
|Gene Model||predicted gene model for transcript(s): [ENSMUST00000119103]|
|Predicted Effect||probably null
|Meta Mutation Damage Score||0.6328|
|Coding Region Coverage||
|Validation Efficiency||98% (54/55)|
FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes an enzyme which oxidizes carotenoids such as beta-carotene during the biosynthesis of vitamin A. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2012]
PHENOTYPE: Mice homozygous for a knock-out allele exhibit increased spleen weight, decreased testis and seminal vesicle weight, and increased tissue lycopene and beta-carotene levels when fed diets containing tomato powder or lycopene. [provided by MGI curators]
|Allele List at MGI|
|Other mutations in this stock||
|Other mutations in Bco2||
(F):5'- CATCCTTGAGATGCTAAAACTAAGG -3'
(R):5'- GGGGTAGACTGGAATTTTCTCCC -3'
(F):5'- TGCTAAAACTAAGGTAGGAATTTGG -3'
(R):5'- CTCCCTTGGAGTATGTGCTCAATG -3'