Mutagenetix > Incidental Mutation

Incidental Mutation 'R5470:Etl4'

433443

Institutional Source

Beutler Lab

Gene Symbol

Etl4

Ensembl Gene

ENSMUSG00000036617

Gene Name

enhancer trap locus 4

Synonyms

6620402G01Rik, Sickle tail, E330027G05Rik, Etl-4, Skt, 9430077C05Rik

MMRRC Submission

043031-MU

Accession Numbers

Essential gene?

Probably essential (E-score: 0.809) question?

Stock #

R5470 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

19915018-20815346 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to T at 20534791 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Histidine to Leucine at position 79 (H79L)

Ref Sequence

ENSEMBL: ENSMUSP00000116637 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000045555] [ENSMUST00000066509] [ENSMUST00000114604] [ENSMUST00000114610] [ENSMUST00000114614] [ENSMUST00000114627] [ENSMUST00000131714] [ENSMUST00000146881]

AlphaFold

A2AQ25

Predicted Effect

possibly damaging
Transcript: ENSMUST00000045555
AA Change: H31L

PolyPhen 2 Score 0.919 (Sensitivity: 0.81; Specificity: 0.94)

SMART Domains

Protein: ENSMUSP00000041431
Gene: ENSMUSG00000036617
AA Change: H31L

Domain	Start	End	E-Value	Type
Pfam:AIP3	188	291	1.7e-11	PFAM
low complexity region	313	328	N/A	INTRINSIC
low complexity region	350	368	N/A	INTRINSIC
coiled coil region	620	652	N/A	INTRINSIC
low complexity region	1067	1096	N/A	INTRINSIC
low complexity region	1212	1231	N/A	INTRINSIC
low complexity region	1296	1314	N/A	INTRINSIC

Predicted Effect

possibly damaging
Transcript: ENSMUST00000066509
AA Change: H31L

PolyPhen 2 Score 0.919 (Sensitivity: 0.81; Specificity: 0.94)

SMART Domains

Protein: ENSMUSP00000066170
Gene: ENSMUSG00000036617
AA Change: H31L

Domain	Start	End	E-Value	Type
low complexity region	313	328	N/A	INTRINSIC
low complexity region	350	368	N/A	INTRINSIC
coiled coil region	655	687	N/A	INTRINSIC
low complexity region	1102	1131	N/A	INTRINSIC
low complexity region	1372	1381	N/A	INTRINSIC
low complexity region	1470	1495	N/A	INTRINSIC
low complexity region	1571	1582	N/A	INTRINSIC
coiled coil region	1658	1686	N/A	INTRINSIC
low complexity region	1724	1737	N/A	INTRINSIC
low complexity region	1806	1825	N/A	INTRINSIC
low complexity region	1890	1908	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000114604
AA Change: H31L

PolyPhen 2 Score 0.280 (Sensitivity: 0.91; Specificity: 0.88)

SMART Domains

Protein: ENSMUSP00000110251
Gene: ENSMUSG00000036617
AA Change: H31L

Domain	Start	End	E-Value	Type
Pfam:AIP3	188	291	1.7e-11	PFAM
low complexity region	313	328	N/A	INTRINSIC
low complexity region	350	368	N/A	INTRINSIC
coiled coil region	655	687	N/A	INTRINSIC
low complexity region	1102	1131	N/A	INTRINSIC
low complexity region	1207	1226	N/A	INTRINSIC
low complexity region	1291	1309	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000114610

SMART Domains

Protein: ENSMUSP00000110257
Gene: ENSMUSG00000036617

Domain	Start	End	E-Value	Type
Pfam:AIP3	108	211	5e-12	PFAM
low complexity region	233	248	N/A	INTRINSIC
low complexity region	270	288	N/A	INTRINSIC

Predicted Effect

possibly damaging
Transcript: ENSMUST00000114614
AA Change: H31L

PolyPhen 2 Score 0.919 (Sensitivity: 0.81; Specificity: 0.94)

SMART Domains

Protein: ENSMUSP00000110261
Gene: ENSMUSG00000036617
AA Change: H31L

Domain	Start	End	E-Value	Type
Pfam:AIP3	188	291	1.7e-11	PFAM
low complexity region	313	328	N/A	INTRINSIC
low complexity region	350	368	N/A	INTRINSIC
coiled coil region	620	652	N/A	INTRINSIC
low complexity region	1056	1085	N/A	INTRINSIC
low complexity region	1201	1220	N/A	INTRINSIC
low complexity region	1285	1303	N/A	INTRINSIC

Predicted Effect

possibly damaging
Transcript: ENSMUST00000114627
AA Change: H82L

PolyPhen 2 Score 0.919 (Sensitivity: 0.81; Specificity: 0.94)

SMART Domains

Protein: ENSMUSP00000110274
Gene: ENSMUSG00000036617
AA Change: H82L

Domain	Start	End	E-Value	Type
low complexity region	21	38	N/A	INTRINSIC
low complexity region	60	72	N/A	INTRINSIC
Pfam:AIP3	239	341	2.4e-14	PFAM
low complexity region	364	379	N/A	INTRINSIC
low complexity region	401	419	N/A	INTRINSIC
Pfam:AIP3	600	841	1.1e-12	PFAM
low complexity region	1153	1182	N/A	INTRINSIC
low complexity region	1423	1432	N/A	INTRINSIC
low complexity region	1521	1546	N/A	INTRINSIC
low complexity region	1622	1633	N/A	INTRINSIC
coiled coil region	1709	1737	N/A	INTRINSIC
low complexity region	1775	1788	N/A	INTRINSIC
low complexity region	1857	1876	N/A	INTRINSIC
low complexity region	1941	1959	N/A	INTRINSIC

Predicted Effect

probably damaging
Transcript: ENSMUST00000131714
AA Change: H79L

PolyPhen 2 Score 0.987 (Sensitivity: 0.73; Specificity: 0.96)

SMART Domains

Protein: ENSMUSP00000116637
Gene: ENSMUSG00000036617
AA Change: H79L

Domain	Start	End	E-Value	Type
low complexity region	18	35	N/A	INTRINSIC
low complexity region	57	69	N/A	INTRINSIC
Blast:THAP	137	167	3e-6	BLAST

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000146613

Predicted Effect

possibly damaging
Transcript: ENSMUST00000146881
AA Change: H82L

PolyPhen 2 Score 0.862 (Sensitivity: 0.83; Specificity: 0.93)

SMART Domains

Protein: ENSMUSP00000119778
Gene: ENSMUSG00000036617
AA Change: H82L

Domain	Start	End	E-Value	Type
low complexity region	21	38	N/A	INTRINSIC
low complexity region	60	72	N/A	INTRINSIC
Blast:THAP	140	170	6e-6	BLAST

Meta Mutation Damage Score

0.0642

Coding Region Coverage

1x: 98.3%
3x: 97.3%
10x: 95.1%
20x: 90.4%

Validation Efficiency

99% (79/80)

MGI Phenotype

PHENOTYPE: Mice homozygous for a gene-trapped allele display malformations of the notochord and caudal vertebrae and may exhibit caudal tail kinks. Mice homozygous for another gene-trapped allele have malformed caudal vertebrae and intervertebral disk abnormalities; about half display kinked tails. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 69 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Actn1	T	A	12: 80,215,715 (GRCm39)	I813F	probably damaging	Het
Aqr	G	A	2: 113,988,056 (GRCm39)	L169F	probably damaging	Het
Atp6v1d	G	A	12: 78,892,058 (GRCm39)	R182C	probably benign	Het
Bcl2l13	G	T	6: 120,839,833 (GRCm39)	A44S	probably benign	Het
Brip1	T	C	11: 86,039,368 (GRCm39)	K389E	possibly damaging	Het
C530025M09Rik	G	A	2: 149,673,045 (GRCm39)		probably benign	Het
Ccdc7b	T	A	8: 129,799,081 (GRCm39)	S53T	possibly damaging	Het
Chd8	A	G	14: 52,450,066 (GRCm39)	F154L	probably damaging	Het
Cop1	A	T	1: 159,094,430 (GRCm39)		probably benign	Het
Cyp2c39	A	G	19: 39,501,974 (GRCm39)	K121R	possibly damaging	Het
Cyp3a57	A	G	5: 145,309,429 (GRCm39)	M256V	probably benign	Het
Deup1	T	C	9: 15,493,916 (GRCm39)		probably null	Het
Dnah10	A	G	5: 124,830,232 (GRCm39)	N709D	probably benign	Het
Dthd1	A	G	5: 62,976,109 (GRCm39)	Y261C	probably damaging	Het
Ear-ps2	G	A	14: 44,284,517 (GRCm39)		noncoding transcript	Het
En2	C	T	5: 28,371,922 (GRCm39)	T133M	probably benign	Het
Endou	A	G	15: 97,616,836 (GRCm39)	F229S	probably damaging	Het
Fah	C	T	7: 84,242,393 (GRCm39)		probably null	Het
Fetub	C	T	16: 22,751,081 (GRCm39)	R143C	probably damaging	Het
Fignl1	T	A	11: 11,752,640 (GRCm39)	E138D	probably benign	Het
Fndc3a	A	G	14: 72,812,008 (GRCm39)	L311P	possibly damaging	Het
Gdf9	T	A	11: 53,327,581 (GRCm39)	V179E	probably benign	Het
Gm14129	G	T	2: 148,769,737 (GRCm39)		noncoding transcript	Het
Gorab	A	G	1: 163,220,078 (GRCm39)	I188T	probably damaging	Het
Gpr152	G	A	19: 4,193,128 (GRCm39)	C223Y	probably damaging	Het
Heatr5b	A	T	17: 79,129,008 (GRCm39)		probably null	Het
Hsd17b3	G	T	13: 64,221,713 (GRCm39)	T104N	probably damaging	Het
Il6ra	C	T	3: 89,793,302 (GRCm39)	V283M	probably benign	Het
Klk1b16	A	T	7: 43,786,755 (GRCm39)	I5F	probably damaging	Het
Krt74	G	A	15: 101,662,900 (GRCm39)		noncoding transcript	Het
Lrch3	T	A	16: 32,818,960 (GRCm39)	N650K	probably damaging	Het
Ly96	A	T	1: 16,779,710 (GRCm39)	E126D	probably benign	Het
Mgarp	C	A	3: 51,298,706 (GRCm39)	R66L	possibly damaging	Het
Naalad2	T	A	9: 18,242,147 (GRCm39)	T586S	probably damaging	Het
Ndufaf3	T	C	9: 108,443,643 (GRCm39)		probably benign	Het
Neb	A	G	2: 52,139,450 (GRCm39)	M3055T	possibly damaging	Het
Neo1	G	T	9: 58,838,350 (GRCm39)	A478D	probably damaging	Het
Nmrk1	A	T	19: 18,617,248 (GRCm39)		probably null	Het
Nsl1	T	C	1: 190,812,737 (GRCm39)	M184T	probably benign	Het
Nup133	T	C	8: 124,657,705 (GRCm39)	N410S	probably benign	Het
Opalin	A	G	19: 41,054,970 (GRCm39)	S75P	probably benign	Het
Or1e17	T	C	11: 73,831,696 (GRCm39)	I208T	probably benign	Het
Or3a4	A	G	11: 73,944,733 (GRCm39)	I284T	possibly damaging	Het
Or51ag1	T	G	7: 103,155,716 (GRCm39)	I146L	probably benign	Het
Palb2	C	A	7: 121,713,574 (GRCm39)	C903F	probably damaging	Het
Pcm1	T	A	8: 41,740,720 (GRCm39)	W989R	probably damaging	Het
Pfkfb4	T	A	9: 108,856,661 (GRCm39)	I389N	probably damaging	Het
Pitrm1	T	C	13: 6,603,306 (GRCm39)	C119R	probably benign	Het
Plekha1	T	C	7: 130,510,106 (GRCm39)	V45A	probably damaging	Het
Pold2	G	A	11: 5,823,048 (GRCm39)	P376S	probably damaging	Het
Rcan2	A	G	17: 44,147,174 (GRCm39)	D4G	probably benign	Het
Slc12a1	A	T	2: 125,012,634 (GRCm39)	T299S	probably damaging	Het
Slc22a29	A	T	19: 8,138,880 (GRCm39)	H527Q	probably benign	Het
Slc22a8	G	A	19: 8,585,234 (GRCm39)	R261H	probably damaging	Het
Slc35e2	C	T	4: 155,694,483 (GRCm39)	P10L	probably benign	Het
Slco4a1	T	C	2: 180,115,907 (GRCm39)	F681S	probably benign	Het
Sorcs2	A	T	5: 36,188,527 (GRCm39)	H860Q	probably benign	Het
Strn	A	T	17: 78,964,374 (GRCm39)	H530Q	probably benign	Het
Tex14	A	G	11: 87,442,430 (GRCm39)	R179G	probably damaging	Het
Tjp3	T	C	10: 81,115,381 (GRCm39)	D350G	probably benign	Het
Tnrc6b	G	T	15: 80,800,912 (GRCm39)	R1406L	possibly damaging	Het
Tnxb	A	G	17: 34,935,947 (GRCm39)	D2666G	probably null	Het
Ttn	A	T	2: 76,560,208 (GRCm39)	Y27652N	probably benign	Het
Utp20	T	A	10: 88,653,758 (GRCm39)	E287D	probably benign	Het
Vmn1r45	T	C	6: 89,910,698 (GRCm39)	I91V	probably benign	Het
Wdr72	A	T	9: 74,046,981 (GRCm39)	K76*	probably null	Het
Zfp689	C	T	7: 127,043,425 (GRCm39)	A402T	probably damaging	Het
Zfpm1	A	G	8: 123,060,532 (GRCm39)	E207G	probably damaging	Het
Zhx2	G	T	15: 57,686,470 (GRCm39)	R613L	possibly damaging	Het

Other mutations in Etl4

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00907:Etl4	APN	2	20,771,289 (GRCm39)	missense	possibly damaging	0.81
IGL00944:Etl4	APN	2	20,534,865 (GRCm39)	missense	possibly damaging	0.52
IGL01078:Etl4	APN	2	20,811,342 (GRCm39)	nonsense	probably null
IGL01099:Etl4	APN	2	20,811,922 (GRCm39)	missense	probably benign	0.06
IGL01337:Etl4	APN	2	20,790,198 (GRCm39)	missense	probably benign	0.01
IGL01348:Etl4	APN	2	20,811,784 (GRCm39)	missense	probably damaging	1.00
IGL01349:Etl4	APN	2	20,718,207 (GRCm39)	missense	probably damaging	1.00
IGL01407:Etl4	APN	2	20,748,667 (GRCm39)	missense	probably damaging	0.99
IGL01552:Etl4	APN	2	20,783,000 (GRCm39)	missense	probably damaging	0.99
IGL01662:Etl4	APN	2	20,811,460 (GRCm39)	missense	probably benign	0.04
IGL01687:Etl4	APN	2	20,534,898 (GRCm39)	missense	probably damaging	1.00
IGL01793:Etl4	APN	2	20,748,709 (GRCm39)	missense	possibly damaging	0.87
IGL01844:Etl4	APN	2	20,811,493 (GRCm39)	missense	probably benign	0.06
IGL02025:Etl4	APN	2	20,811,337 (GRCm39)	missense	probably damaging	1.00
IGL02088:Etl4	APN	2	20,811,359 (GRCm39)	missense	probably damaging	1.00
IGL02134:Etl4	APN	2	20,811,240 (GRCm39)	missense	possibly damaging	0.79
IGL02369:Etl4	APN	2	20,535,000 (GRCm39)	missense	probably damaging	1.00
IGL02480:Etl4	APN	2	20,793,335 (GRCm39)	missense	probably damaging	0.99
IGL02560:Etl4	APN	2	20,748,529 (GRCm39)	missense	probably damaging	1.00
IGL02851:Etl4	APN	2	20,812,840 (GRCm39)	missense	possibly damaging	0.46
IGL02893:Etl4	APN	2	20,765,021 (GRCm39)	splice site	probably benign
IGL02951:Etl4	APN	2	20,806,348 (GRCm39)	splice site	probably benign
IGL03119:Etl4	APN	2	20,718,198 (GRCm39)	missense	probably damaging	1.00
IGL03267:Etl4	APN	2	20,789,993 (GRCm39)	nonsense	probably null
IGL03379:Etl4	APN	2	20,666,827 (GRCm39)	missense	possibly damaging	0.87
R0038:Etl4	UTSW	2	20,748,385 (GRCm39)	missense	probably damaging	1.00
R0038:Etl4	UTSW	2	20,748,385 (GRCm39)	missense	probably damaging	1.00
R0095:Etl4	UTSW	2	20,748,679 (GRCm39)	missense	probably damaging	1.00
R0100:Etl4	UTSW	2	20,344,716 (GRCm39)	missense	probably benign
R0311:Etl4	UTSW	2	20,811,940 (GRCm39)	missense	probably damaging	1.00
R0346:Etl4	UTSW	2	20,764,463 (GRCm39)	critical splice donor site	probably null
R0348:Etl4	UTSW	2	20,782,940 (GRCm39)	missense	probably damaging	1.00
R0379:Etl4	UTSW	2	20,812,165 (GRCm39)	missense	probably damaging	0.98
R0571:Etl4	UTSW	2	20,748,580 (GRCm39)	missense	probably damaging	0.99
R0697:Etl4	UTSW	2	20,748,672 (GRCm39)	missense	probably damaging	1.00
R0707:Etl4	UTSW	2	20,810,382 (GRCm39)	splice site	probably benign
R0980:Etl4	UTSW	2	20,806,378 (GRCm39)	missense	probably damaging	1.00
R1120:Etl4	UTSW	2	20,811,514 (GRCm39)	missense	probably benign	0.00
R1254:Etl4	UTSW	2	20,812,734 (GRCm39)	missense	probably damaging	1.00
R1346:Etl4	UTSW	2	20,810,955 (GRCm39)	missense	possibly damaging	0.94
R1460:Etl4	UTSW	2	20,793,288 (GRCm39)	missense	probably damaging	1.00
R1503:Etl4	UTSW	2	20,748,685 (GRCm39)	missense	possibly damaging	0.94
R1547:Etl4	UTSW	2	20,790,039 (GRCm39)	missense	probably damaging	1.00
R1627:Etl4	UTSW	2	20,806,390 (GRCm39)	missense	possibly damaging	0.91
R1635:Etl4	UTSW	2	20,811,219 (GRCm39)	missense	probably damaging	1.00
R1716:Etl4	UTSW	2	20,748,492 (GRCm39)	missense	probably damaging	1.00
R1795:Etl4	UTSW	2	20,812,837 (GRCm39)	critical splice donor site	probably null
R1885:Etl4	UTSW	2	20,748,795 (GRCm39)	missense	probably damaging	1.00
R2039:Etl4	UTSW	2	20,790,039 (GRCm39)	missense	probably damaging	1.00
R2083:Etl4	UTSW	2	20,748,360 (GRCm39)	missense	probably damaging	1.00
R2109:Etl4	UTSW	2	20,790,153 (GRCm39)	missense	probably benign	0.27
R2153:Etl4	UTSW	2	20,803,545 (GRCm39)	missense	probably benign	0.00
R2403:Etl4	UTSW	2	20,812,117 (GRCm39)	nonsense	probably null
R2883:Etl4	UTSW	2	20,810,985 (GRCm39)	missense	possibly damaging	0.83
R2985:Etl4	UTSW	2	20,786,660 (GRCm39)	missense	probably damaging	1.00
R3402:Etl4	UTSW	2	20,786,693 (GRCm39)	missense	probably damaging	1.00
R3696:Etl4	UTSW	2	20,806,473 (GRCm39)	critical splice donor site	probably null
R3755:Etl4	UTSW	2	20,748,348 (GRCm39)	missense	probably benign	0.10
R3813:Etl4	UTSW	2	20,793,246 (GRCm39)	missense	probably damaging	1.00
R3829:Etl4	UTSW	2	20,790,232 (GRCm39)	missense	probably benign	0.07
R3887:Etl4	UTSW	2	20,534,772 (GRCm39)	nonsense	probably null
R3888:Etl4	UTSW	2	20,534,772 (GRCm39)	nonsense	probably null
R3889:Etl4	UTSW	2	20,534,772 (GRCm39)	nonsense	probably null
R3958:Etl4	UTSW	2	20,344,854 (GRCm39)	missense	probably benign
R3959:Etl4	UTSW	2	20,344,854 (GRCm39)	missense	probably benign
R3960:Etl4	UTSW	2	20,344,854 (GRCm39)	missense	probably benign
R4058:Etl4	UTSW	2	20,810,830 (GRCm39)	missense	possibly damaging	0.59
R4074:Etl4	UTSW	2	20,814,030 (GRCm39)	utr 3 prime	probably benign
R4077:Etl4	UTSW	2	20,812,772 (GRCm39)	missense	probably damaging	1.00
R4078:Etl4	UTSW	2	20,812,772 (GRCm39)	missense	probably damaging	1.00
R4127:Etl4	UTSW	2	20,748,886 (GRCm39)	missense	possibly damaging	0.93
R4200:Etl4	UTSW	2	20,786,694 (GRCm39)	missense	probably damaging	1.00
R4492:Etl4	UTSW	2	20,811,676 (GRCm39)	missense	possibly damaging	0.67
R4514:Etl4	UTSW	2	20,666,709 (GRCm39)	missense	probably damaging	1.00
R4820:Etl4	UTSW	2	20,811,496 (GRCm39)	missense	possibly damaging	0.85
R4825:Etl4	UTSW	2	20,811,738 (GRCm39)	missense	probably damaging	1.00
R4888:Etl4	UTSW	2	20,344,922 (GRCm39)	critical splice donor site	probably null
R4938:Etl4	UTSW	2	20,803,460 (GRCm39)	missense	probably benign	0.00
R4943:Etl4	UTSW	2	20,812,092 (GRCm39)	missense	probably benign	0.05
R5121:Etl4	UTSW	2	20,344,922 (GRCm39)	critical splice donor site	probably null
R5191:Etl4	UTSW	2	20,344,810 (GRCm39)	missense	probably damaging	0.99
R5198:Etl4	UTSW	2	20,718,198 (GRCm39)	missense	probably damaging	1.00
R5199:Etl4	UTSW	2	20,748,853 (GRCm39)	missense	probably damaging	1.00
R5513:Etl4	UTSW	2	20,748,638 (GRCm39)	missense	probably damaging	1.00
R5620:Etl4	UTSW	2	20,535,037 (GRCm39)	missense	probably damaging	1.00
R5635:Etl4	UTSW	2	20,811,846 (GRCm39)	missense	probably damaging	1.00
R5641:Etl4	UTSW	2	20,811,273 (GRCm39)	frame shift	probably null
R5690:Etl4	UTSW	2	20,810,647 (GRCm39)	missense	probably benign	0.01
R5784:Etl4	UTSW	2	20,811,016 (GRCm39)	missense	possibly damaging	0.79
R5794:Etl4	UTSW	2	20,811,323 (GRCm39)	missense	probably damaging	1.00
R5908:Etl4	UTSW	2	20,748,718 (GRCm39)	missense	probably damaging	0.96
R5982:Etl4	UTSW	2	20,785,826 (GRCm39)	missense	probably damaging	1.00
R6151:Etl4	UTSW	2	20,718,171 (GRCm39)	missense	probably damaging	1.00
R6192:Etl4	UTSW	2	20,806,362 (GRCm39)	missense	probably damaging	0.98
R6238:Etl4	UTSW	2	20,806,379 (GRCm39)	missense	probably damaging	1.00
R6248:Etl4	UTSW	2	20,813,900 (GRCm39)	missense	possibly damaging	0.90
R6292:Etl4	UTSW	2	20,748,384 (GRCm39)	missense	probably damaging	1.00
R6610:Etl4	UTSW	2	20,718,180 (GRCm39)	missense	probably damaging	1.00
R6739:Etl4	UTSW	2	20,718,246 (GRCm39)	missense	probably damaging	1.00
R6846:Etl4	UTSW	2	20,748,919 (GRCm39)	missense	possibly damaging	0.94
R6863:Etl4	UTSW	2	20,811,120 (GRCm39)	missense	probably benign	0.01
R6873:Etl4	UTSW	2	20,802,803 (GRCm39)	splice site	probably null
R7003:Etl4	UTSW	2	20,810,695 (GRCm39)	missense	probably benign	0.03
R7155:Etl4	UTSW	2	20,811,742 (GRCm39)	missense	probably damaging	0.96
R7207:Etl4	UTSW	2	20,714,387 (GRCm39)	missense	probably damaging	0.99
R7230:Etl4	UTSW	2	20,802,799 (GRCm39)	missense	probably damaging	1.00
R7305:Etl4	UTSW	2	20,714,368 (GRCm39)	missense	probably damaging	1.00
R7389:Etl4	UTSW	2	20,789,904 (GRCm39)	nonsense	probably null
R7396:Etl4	UTSW	2	20,803,449 (GRCm39)	missense	possibly damaging	0.62
R7441:Etl4	UTSW	2	20,749,000 (GRCm39)	missense	possibly damaging	0.87
R7626:Etl4	UTSW	2	20,718,189 (GRCm39)	missense	probably damaging	1.00
R7776:Etl4	UTSW	2	20,811,957 (GRCm39)	missense	probably damaging	0.99
R7779:Etl4	UTSW	2	20,714,288 (GRCm39)	missense	probably damaging	1.00
R7798:Etl4	UTSW	2	20,786,757 (GRCm39)	critical splice donor site	probably null
R7851:Etl4	UTSW	2	20,748,951 (GRCm39)	missense	probably damaging	1.00
R7861:Etl4	UTSW	2	20,810,721 (GRCm39)	missense	probably benign
R7901:Etl4	UTSW	2	20,294,821 (GRCm39)	missense	possibly damaging	0.83
R8053:Etl4	UTSW	2	20,666,774 (GRCm39)	missense	probably damaging	1.00
R8124:Etl4	UTSW	2	20,811,451 (GRCm39)	missense	probably benign	0.06
R8133:Etl4	UTSW	2	20,811,082 (GRCm39)	missense	possibly damaging	0.86
R8203:Etl4	UTSW	2	20,789,916 (GRCm39)	missense	possibly damaging	0.61
R8238:Etl4	UTSW	2	20,811,342 (GRCm39)	nonsense	probably null
R8263:Etl4	UTSW	2	20,748,965 (GRCm39)	missense	probably benign	0.00
R8299:Etl4	UTSW	2	20,748,874 (GRCm39)	missense	possibly damaging	0.81
R8318:Etl4	UTSW	2	20,793,341 (GRCm39)	missense	probably damaging	1.00
R8334:Etl4	UTSW	2	20,785,857 (GRCm39)	missense	probably damaging	0.96
R8443:Etl4	UTSW	2	20,810,977 (GRCm39)	missense	probably benign	0.04
R8525:Etl4	UTSW	2	20,534,892 (GRCm39)	missense	probably damaging	1.00
R8679:Etl4	UTSW	2	20,714,288 (GRCm39)	missense	probably damaging	1.00
R8918:Etl4	UTSW	2	20,811,246 (GRCm39)	missense	probably benign	0.00
R8918:Etl4	UTSW	2	20,748,733 (GRCm39)	missense	probably benign
R9062:Etl4	UTSW	2	20,748,616 (GRCm39)	missense	probably damaging	0.99
R9095:Etl4	UTSW	2	20,782,964 (GRCm39)	missense	probably damaging	1.00
R9200:Etl4	UTSW	2	20,786,702 (GRCm39)	missense	probably damaging	1.00
R9416:Etl4	UTSW	2	20,748,784 (GRCm39)	missense	probably benign	0.17
R9437:Etl4	UTSW	2	20,813,872 (GRCm39)	missense	probably benign	0.20
R9451:Etl4	UTSW	2	20,813,926 (GRCm39)	missense	probably benign	0.03
R9489:Etl4	UTSW	2	20,771,345 (GRCm39)	missense	possibly damaging	0.75
R9531:Etl4	UTSW	2	20,294,818 (GRCm39)	start codon destroyed	probably null	0.01
R9605:Etl4	UTSW	2	20,771,345 (GRCm39)	missense	possibly damaging	0.75
R9623:Etl4	UTSW	2	20,811,052 (GRCm39)	missense
R9631:Etl4	UTSW	2	20,666,749 (GRCm39)	missense	probably benign	0.28
R9632:Etl4	UTSW	2	20,666,749 (GRCm39)	missense	probably benign	0.28
R9646:Etl4	UTSW	2	20,802,724 (GRCm39)	missense	probably benign	0.00
R9732:Etl4	UTSW	2	20,748,373 (GRCm39)	missense	probably damaging	0.98
R9755:Etl4	UTSW	2	20,790,048 (GRCm39)	missense	probably benign	0.17
R9771:Etl4	UTSW	2	20,811,537 (GRCm39)	missense	probably benign
RF003:Etl4	UTSW	2	20,524,729 (GRCm39)	nonsense	probably null
X0018:Etl4	UTSW	2	20,814,001 (GRCm39)	missense	probably damaging	0.98
X0022:Etl4	UTSW	2	20,714,375 (GRCm39)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- AAACAACCGGTTTCTCTGACC -3'
(R):5'- GTTCTAGGAACGCCTCTCTC -3'

Sequencing Primer
(F):5'- CTGGAACTCACTTTGTAGACCAGG -3'
(R):5'- AGGAACGCCTCTCTCTTCCTG -3'

Posted On

2016-10-06