Mutagenetix > Incidental Mutation

Incidental Mutation 'R5537:Psma5-ps'

434884

Institutional Source

Beutler Lab

Gene Symbol

Psma5-ps

Ensembl Gene

ENSMUSG00000050490

Gene Name

proteasome subunit alpha 5, pseudogene

Synonyms

Gm8394

MMRRC Submission

043095-MU

Accession Numbers

Essential gene?

Probably essential (E-score: 0.905) question?

Stock #

R5537 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

85149352-85150303 bp(+) (GRCm39)

Type of Mutation

exon

DNA Base Change (assembly)

A to G at 85149913 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Gene Model

predicted gene model for transcript(s):

AlphaFold

no structure available at present

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000079684

SMART Domains

Protein: ENSMUSP00000078625
Gene: ENSMUSG00000050490

Domain	Start	End	E-Value	Type
Proteasome_A_N	8	30	6.32e-8	SMART
Pfam:Proteasome	31	220	5.6e-57	PFAM

Coding Region Coverage

1x: 99.9%
3x: 99.7%
10x: 98.3%
20x: 95.1%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 43 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Abcb4	A	T	5: 9,005,485 (GRCm39)	I1123F	probably damaging	Het
Acsl3	C	T	1: 78,684,073 (GRCm39)	R702C	probably damaging	Het
Ank1	A	G	8: 23,604,892 (GRCm39)	E1031G	probably damaging	Het
Ankle2	T	C	5: 110,397,361 (GRCm39)	S536P	probably damaging	Het
Arfgef2	G	T	2: 166,698,513 (GRCm39)		probably null	Het
Bahd1	G	T	2: 118,746,461 (GRCm39)	D27Y	probably damaging	Het
Brinp2	C	T	1: 158,082,583 (GRCm39)	V246I	probably damaging	Het
Ccdc187	C	A	2: 26,166,237 (GRCm39)	A731S	probably benign	Het
Cdc37l1	T	A	19: 28,972,518 (GRCm39)	N70K	probably damaging	Het
Cdk5r1	A	G	11: 80,368,825 (GRCm39)	Y164C	probably damaging	Het
Clrn3	A	T	7: 135,115,791 (GRCm39)	H186Q	probably benign	Het
Col12a1	G	A	9: 79,606,872 (GRCm39)	T517I	probably damaging	Het
Crhr1	T	A	11: 104,054,682 (GRCm39)	N98K	possibly damaging	Het
Dst	C	T	1: 34,228,959 (GRCm39)	S2362L	probably benign	Het
Eapp	G	A	12: 54,738,844 (GRCm39)	T98I	probably benign	Het
Iqsec3	T	C	6: 121,389,603 (GRCm39)		probably benign	Het
Kctd12	A	G	14: 103,219,713 (GRCm39)	V55A	probably benign	Het
Kmt2d	G	A	15: 98,749,990 (GRCm39)		probably benign	Het
Lgr5	T	C	10: 115,292,594 (GRCm39)	D432G	probably benign	Het
Mrgprx1	T	A	7: 47,670,898 (GRCm39)	H283L	probably benign	Het
Nlrp4e	T	C	7: 23,019,914 (GRCm39)	F134L	probably benign	Het
Or1e34	T	C	11: 73,778,523 (GRCm39)	K225R	probably benign	Het
Or4f14b	A	T	2: 111,775,393 (GRCm39)	M136K	probably damaging	Het
Or5m5	T	A	2: 85,814,570 (GRCm39)	Y129N	possibly damaging	Het
Or8b43	T	A	9: 38,360,538 (GRCm39)	Y123*	probably null	Het
Pfkp	C	A	13: 6,669,278 (GRCm39)	W235L	probably damaging	Het
Prom1	C	T	5: 44,158,118 (GRCm39)		probably null	Het
Rab14	A	T	2: 35,082,649 (GRCm39)	F12L	possibly damaging	Het
Rnf40	T	A	7: 127,195,261 (GRCm39)	I429N	probably benign	Het
S100pbp	G	A	4: 129,075,981 (GRCm39)	P115S	probably benign	Het
Scarf1	G	A	11: 75,416,357 (GRCm39)	A600T	probably damaging	Het
Slc6a5	T	C	7: 49,609,059 (GRCm39)	F755L	probably benign	Het
Slc7a2	A	T	8: 41,367,023 (GRCm39)	I510F	probably benign	Het
Spag16	T	C	1: 69,866,175 (GRCm39)	S5P	probably benign	Het
Tex15	A	G	8: 34,061,641 (GRCm39)	Y631C	probably damaging	Het
Tmem182	A	T	1: 40,894,069 (GRCm39)	I195F	probably benign	Het
Tox	A	G	4: 6,697,510 (GRCm39)	L431P	probably damaging	Het
Tprn	G	A	2: 25,153,369 (GRCm39)	A224T	probably benign	Het
Tsg101	T	C	7: 46,540,876 (GRCm39)	K294E	probably benign	Het
Tubb2a	C	A	13: 34,259,434 (GRCm39)	V119L	probably benign	Het
Vps37d	C	T	5: 135,103,256 (GRCm39)	E129K	possibly damaging	Het
Zfp974	TAATCTGTCTCCAAATCTG	TAATCTG	7: 27,611,671 (GRCm39)		probably benign	Het
Zpld1	A	G	16: 55,054,003 (GRCm39)	V263A	possibly damaging	Het

Other mutations in Psma5-ps

Allele	Source	Chr	Coord	Type	Predicted Effect
IGL01336:Psma5-ps	APN	10	85,150,028 (GRCm39)	exon	noncoding transcript
IGL01467:Psma5-ps	APN	10	85,149,986 (GRCm39)	exon	noncoding transcript
IGL02638:Psma5-ps	APN	10	85,149,698 (GRCm39)	exon	noncoding transcript
IGL03253:Psma5-ps	APN	10	85,149,556 (GRCm39)	exon	noncoding transcript
R1929:Psma5-ps	UTSW	10	85,149,595 (GRCm39)	exon	noncoding transcript
R2271:Psma5-ps	UTSW	10	85,149,595 (GRCm39)	exon	noncoding transcript
R2893:Psma5-ps	UTSW	10	85,149,848 (GRCm39)	exon	noncoding transcript
R4689:Psma5-ps	UTSW	10	85,150,065 (GRCm39)	exon	noncoding transcript
R4711:Psma5-ps	UTSW	10	85,149,667 (GRCm39)	exon	noncoding transcript
R5934:Psma5-ps	UTSW	10	85,150,145 (GRCm39)	exon	noncoding transcript
T0722:Psma5-ps	UTSW	10	85,149,457 (GRCm39)	exon	noncoding transcript

Predicted Primers

PCR Primer
(F):5'- TGGGCTAATTGCTGATGCTAAAAC -3'
(R):5'- TGGAAGTTCTGACCAGGCTG -3'

Sequencing Primer
(F):5'- ACCACTGGTTCACCTATAATGAG -3'
(R):5'- TTCTGACCAGGCTGCACCG -3'

Posted On

2016-10-24