Mutagenetix > Incidental Mutation

Incidental Mutation 'R0190:Or13f5'

43506

Institutional Source

Beutler Lab

Gene Symbol

Or13f5

Ensembl Gene

ENSMUSG00000089717

Gene Name

olfactory receptor family 13 subfamily F member 5

Synonyms

GA_x6K02T2N78B-7168533-7167574, Olfr275, MOR262-2

MMRRC Submission

038451-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.085) question?

Stock #

R0190 (G1)

Quality Score

Status

Not validated

Chromosome

Chromosomal Location

52825399-52826358 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

G to C at 52825613 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Tryptophan to Serine at position 72 (W72S)

Ref Sequence

ENSEMBL: ENSMUSP00000092700 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000095085]

AlphaFold

Q7TS18

Predicted Effect

probably damaging
Transcript: ENSMUST00000095085
AA Change: W72S

PolyPhen 2 Score 0.972 (Sensitivity: 0.77; Specificity: 0.96)

SMART Domains

Protein: ENSMUSP00000092700
Gene: ENSMUSG00000089717
AA Change: W72S

Domain	Start	End	E-Value	Type
Pfam:7tm_4	30	308	9.8e-54	PFAM
Pfam:7tm_1	41	290	6.2e-20	PFAM

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000120435

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000135738

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000219705

Coding Region Coverage

1x: 98.8%
3x: 97.6%
10x: 91.6%
20x: 72.5%

Validation Efficiency

75% (45/60)

MGI Phenotype

FUNCTION: Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]

Allele List at MGI

Other mutations in this stock

Total: 61 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Acsl1	T	C	8: 46,966,429 (GRCm39)		probably null	Het
Aff2	CA	CAAA	X: 68,892,711 (GRCm39)		probably null	Het
Ankrd34a	A	G	3: 96,505,105 (GRCm39)	D103G	probably damaging	Het
Atp1b2	T	C	11: 69,492,388 (GRCm39)	D224G	probably damaging	Het
Atxn10	A	G	15: 85,220,730 (GRCm39)	D22G	possibly damaging	Het
Btbd9	C	T	17: 30,493,916 (GRCm39)	D492N	possibly damaging	Het
Caskin1	C	T	17: 24,723,596 (GRCm39)	L795F	possibly damaging	Het
Cdk12	T	C	11: 98,132,657 (GRCm39)		probably null	Het
Crtc2	A	G	3: 90,166,716 (GRCm39)	H91R	probably damaging	Het
Dbt	A	G	3: 116,332,736 (GRCm39)		probably null	Het
Dda1	C	A	8: 71,924,877 (GRCm39)	Y41*	probably null	Het
Dnah2	T	A	11: 69,326,075 (GRCm39)	D3692V	probably damaging	Het
Dpep1	A	G	8: 123,927,447 (GRCm39)	T334A	probably benign	Het
Enthd1	C	T	15: 80,418,695 (GRCm39)		probably null	Het
Fpr-rs6	T	A	17: 20,402,741 (GRCm39)	I207F	probably benign	Het
Fsip2	T	A	2: 82,815,521 (GRCm39)	S3751R	possibly damaging	Het
Gigyf2	A	T	1: 87,356,410 (GRCm39)		probably benign	Het
Gtf3c4	C	A	2: 28,730,140 (GRCm39)	D34Y	probably benign	Het
Iftap	G	A	2: 101,416,775 (GRCm39)	S58L	probably benign	Het
Igfn1	A	T	1: 135,889,790 (GRCm39)	V2419E	probably damaging	Het
Kank1	A	T	19: 25,386,647 (GRCm39)	I79L	probably benign	Het
Kif21b	A	G	1: 136,098,957 (GRCm39)	H1415R	probably benign	Het
Mad2l1	T	C	6: 66,516,862 (GRCm39)	S185P	possibly damaging	Het
Mettl18	A	G	1: 163,823,991 (GRCm39)	E104G	probably damaging	Het
Mrgprb2	G	A	7: 48,202,525 (GRCm39)	H67Y	possibly damaging	Het
Mrgprd	G	A	7: 144,875,439 (GRCm39)	M103I	probably benign	Het
Nanos3	C	T	8: 84,902,763 (GRCm39)	R133Q	probably damaging	Het
Npc1	G	A	18: 12,324,887 (GRCm39)	T1202I	probably damaging	Het
Nucks1	A	G	1: 131,852,329 (GRCm39)	D60G	probably damaging	Het
Or10g7	A	G	9: 39,905,840 (GRCm39)	I245V	probably benign	Het
Or4a72	T	A	2: 89,405,302 (GRCm39)	Y256F	probably damaging	Het
Paqr8	A	G	1: 21,005,271 (GRCm39)	T142A	probably benign	Het
Pdss1	T	C	2: 22,796,843 (GRCm39)	S119P	probably damaging	Het
Plcl2	A	G	17: 50,914,671 (GRCm39)	D560G	probably benign	Het
Ppm1b	T	A	17: 85,301,531 (GRCm39)	V137E	probably damaging	Het
Ppp1r16b	A	C	2: 158,537,983 (GRCm39)	K35Q	probably damaging	Het
Prkd2	A	T	7: 16,603,815 (GRCm39)	E832V	probably damaging	Het
Rab34	G	T	11: 78,082,232 (GRCm39)	K191N	possibly damaging	Het
Rad51ap2	A	C	12: 11,508,540 (GRCm39)	T821P	probably benign	Het
Rbm19	A	G	5: 120,282,111 (GRCm39)	T823A	probably benign	Het
Rpf2	T	G	10: 40,103,597 (GRCm39)	H106P	probably damaging	Het
Schip1	A	G	3: 68,533,177 (GRCm39)	M453V	probably benign	Het
Sema5a	T	A	15: 32,562,920 (GRCm39)	N310K	possibly damaging	Het
Sf3b1	T	C	1: 55,029,465 (GRCm39)	D1179G	probably damaging	Het
Skint2	A	T	4: 112,473,729 (GRCm39)	T4S	possibly damaging	Het
Slc22a5	A	T	11: 53,760,241 (GRCm39)	Y358*	probably null	Het
Slc34a1	T	C	13: 55,556,914 (GRCm39)	M251T	probably benign	Het
Slc44a5	A	G	3: 153,944,755 (GRCm39)	D124G	probably null	Het
Slc9b1	G	A	3: 135,063,434 (GRCm39)	E73K	unknown	Het
Ssbp2	T	C	13: 91,817,829 (GRCm39)	L156P	probably damaging	Het
Taar2	G	A	10: 23,817,393 (GRCm39)	R311H	probably benign	Het
Trim47	A	G	11: 115,997,053 (GRCm39)	V568A	probably damaging	Het
Ttn	A	T	2: 76,718,324 (GRCm39)		probably benign	Het
Ttpa	A	T	4: 20,021,260 (GRCm39)	I74F	probably damaging	Het
Vmn2r52	T	C	7: 9,905,315 (GRCm39)	I175V	probably benign	Het
Wrn	C	T	8: 33,731,011 (GRCm39)	C1350Y	probably benign	Het
Zfp11	C	T	5: 129,735,302 (GRCm39)	G53E	possibly damaging	Het
Zfp422	A	T	6: 116,603,572 (GRCm39)	D142E	probably damaging	Het
Zfp473	A	T	7: 44,382,612 (GRCm39)	C574S	probably damaging	Het
Zfp638	T	A	6: 83,905,946 (GRCm39)	M37K	probably damaging	Het
Zfp976	C	A	7: 42,291,948 (GRCm39)		probably benign	Het

Other mutations in Or13f5

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01108:Or13f5	APN	4	52,825,727 (GRCm39)	missense	probably damaging	1.00
IGL01758:Or13f5	APN	4	52,825,468 (GRCm39)	nonsense	probably null
IGL01925:Or13f5	APN	4	52,825,910 (GRCm39)	missense	probably benign	0.00
IGL02525:Or13f5	APN	4	52,825,616 (GRCm39)	missense	probably damaging	1.00
IGL02536:Or13f5	APN	4	52,825,817 (GRCm39)	missense	possibly damaging	0.95
IGL02829:Or13f5	APN	4	52,826,027 (GRCm39)	missense	probably damaging	0.98
R0068:Or13f5	UTSW	4	52,825,503 (GRCm39)	nonsense	probably null
R0068:Or13f5	UTSW	4	52,825,503 (GRCm39)	nonsense	probably null
R4376:Or13f5	UTSW	4	52,826,195 (GRCm39)	missense	possibly damaging	0.81
R4617:Or13f5	UTSW	4	52,825,399 (GRCm39)	start codon destroyed	probably benign	0.35
R4658:Or13f5	UTSW	4	52,826,240 (GRCm39)	missense	probably damaging	0.99
R4828:Or13f5	UTSW	4	52,826,138 (GRCm39)	missense	probably damaging	1.00
R4850:Or13f5	UTSW	4	52,825,450 (GRCm39)	missense	possibly damaging	0.94
R6194:Or13f5	UTSW	4	52,825,779 (GRCm39)	nonsense	probably null
R6401:Or13f5	UTSW	4	52,826,242 (GRCm39)	missense	probably damaging	1.00
R6842:Or13f5	UTSW	4	52,825,576 (GRCm39)	missense	probably damaging	1.00
R7033:Or13f5	UTSW	4	52,826,089 (GRCm39)	missense	probably benign
R7998:Or13f5	UTSW	4	52,825,970 (GRCm39)	missense	possibly damaging	0.89
R8101:Or13f5	UTSW	4	52,825,849 (GRCm39)	missense	probably benign	0.03
R9655:Or13f5	UTSW	4	52,825,526 (GRCm39)	missense	probably benign

Predicted Primers

PCR Primer
(F):5'- TTCGCACTACCCCAGAATTGAAGTC -3'
(R):5'- TCACAGGTCCAGTTTCCACCAAGG -3'

Sequencing Primer
(F):5'- CCCAGAATTGAAGTCACCGTATTTG -3'
(R):5'- TTTCCACCAAGGCAGTGAG -3'

Posted On

2013-05-24