Incidental Mutation 'R5554:Stk4'
ID 435262
Institutional Source Beutler Lab
Gene Symbol Stk4
Ensembl Gene ENSMUSG00000018209
Gene Name serine/threonine kinase 4
Synonyms sterile 20-like kinase 1, Kas-2, Ysk3, Mst1
MMRRC Submission 043111-MU
Accession Numbers
Essential gene? Non essential (E-score: 0.000) question?
Stock # R5554 (G1)
Quality Score 225
Status Validated
Chromosome 2
Chromosomal Location 163916033-163997444 bp(+) (GRCm39)
Type of Mutation missense
DNA Base Change (assembly) T to A at 163941645 bp (GRCm39)
Zygosity Heterozygous
Amino Acid Change Valine to Glutamic Acid at position 287 (V287E)
Ref Sequence ENSEMBL: ENSMUSP00000018353 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000018353]
AlphaFold Q9JI11
Predicted Effect probably benign
Transcript: ENSMUST00000018353
AA Change: V287E

PolyPhen 2 Score 0.000 (Sensitivity: 1.00; Specificity: 0.00)
SMART Domains Protein: ENSMUSP00000018353
Gene: ENSMUSG00000018209
AA Change: V287E

DomainStartEndE-ValueType
S_TKc 30 281 1.97e-104 SMART
low complexity region 311 326 N/A INTRINSIC
Pfam:Mst1_SARAH 433 480 2.4e-26 PFAM
Predicted Effect unknown
Transcript: ENSMUST00000137866
AA Change: V2E
SMART Domains Protein: ENSMUSP00000116745
Gene: ENSMUSG00000018209
AA Change: V2E

DomainStartEndE-ValueType
Blast:S_TKc 2 26 8e-6 BLAST
PDB:3COM|B 2 26 4e-7 PDB
low complexity region 27 42 N/A INTRINSIC
Predicted Effect noncoding transcript
Transcript: ENSMUST00000153319
Meta Mutation Damage Score 0.0690 question?
Coding Region Coverage
  • 1x: 99.9%
  • 3x: 99.7%
  • 10x: 98.6%
  • 20x: 96.1%
Validation Efficiency 97% (73/75)
MGI Phenotype FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] The protein encoded by this gene is a cytoplasmic kinase that is structurally similar to the yeast Ste20p kinase, which acts upstream of the stress-induced mitogen-activated protein kinase cascade. The encoded protein can phosphorylate myelin basic protein and undergoes autophosphorylation. A caspase-cleaved fragment of the encoded protein has been shown to be capable of phosphorylating histone H2B. The particular phosphorylation catalyzed by this protein has been correlated with apoptosis, and it's possible that this protein induces the chromatin condensation observed in this process. [provided by RefSeq, Jul 2008]
PHENOTYPE: Mice homozygous for a gene trap allele have low numbers of na�ve T cells that are hyper-responsive to stimulation. Mice homozygous for knock-out alleles exhibit decreased peripheral T cell numbers due to impaired emigration and homing. [provided by MGI curators]
Allele List at MGI
Other mutations in this stock
Total: 68 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
Aarsd1 C A 11: 101,304,807 (GRCm39) R227L probably benign Het
Adamtsl1 A G 4: 86,195,182 (GRCm39) Q533R possibly damaging Het
Adgb C T 10: 10,216,217 (GRCm39) R1524H probably damaging Het
Ank2 A T 3: 126,792,622 (GRCm39) N739K possibly damaging Het
Ankrd39 C T 1: 36,581,062 (GRCm39) G96R probably damaging Het
Anxa10 G A 8: 62,514,080 (GRCm39) P249L possibly damaging Het
Banp G A 8: 122,718,334 (GRCm39) E183K probably damaging Het
BC051665 C G 13: 60,932,435 (GRCm39) L83F probably damaging Het
Btn1a1 A T 13: 23,643,295 (GRCm39) F385I possibly damaging Het
Chchd4 A T 6: 91,441,999 (GRCm39) *140R probably null Het
Dcpp2 T C 17: 24,119,545 (GRCm39) Y120H probably damaging Het
Dlx2 G A 2: 71,375,805 (GRCm39) R173C possibly damaging Het
Dmbt1 C A 7: 130,701,030 (GRCm39) Y1069* probably null Het
Dop1a T A 9: 86,403,710 (GRCm39) F1637I probably damaging Het
Dusp18 T C 11: 3,847,202 (GRCm39) I64T probably damaging Het
Evi5l A G 8: 4,256,491 (GRCm39) probably benign Het
Fiz1 T C 7: 5,015,849 (GRCm39) H47R probably damaging Het
Fndc3b G T 3: 27,697,162 (GRCm39) P17T possibly damaging Het
Foxa1 T A 12: 57,589,077 (GRCm39) Q381L probably benign Het
Gda A G 19: 21,405,837 (GRCm39) probably null Het
Gm10044 T C 14: 7,771,181 (GRCm38) noncoding transcript Het
Gm12258 T A 11: 58,749,294 (GRCm39) S156R possibly damaging Het
Gm973 A G 1: 59,566,131 (GRCm39) R117G probably benign Het
Grwd1 C T 7: 45,480,064 (GRCm39) V48I probably damaging Het
Ifi209 T A 1: 173,468,763 (GRCm39) S198T probably benign Het
Inka2 T A 3: 105,623,930 (GRCm39) S82R possibly damaging Het
Itga1 A T 13: 115,129,010 (GRCm39) C549* probably null Het
Kmt2c C A 5: 25,499,608 (GRCm39) G511C probably damaging Het
Knstrn T C 2: 118,664,444 (GRCm39) probably benign Het
Lrp2 T C 2: 69,382,768 (GRCm39) Y39C possibly damaging Het
Maco1 T C 4: 134,555,445 (GRCm39) I343V probably benign Het
Micos10 T C 4: 138,833,218 (GRCm39) probably benign Het
N4bp2 T C 5: 65,965,457 (GRCm39) Y1169H probably benign Het
Nbr1 C T 11: 101,455,633 (GRCm39) T129I probably benign Het
Or52e8 A C 7: 104,625,189 (GRCm39) M1R probably null Het
Or5k15 A T 16: 58,710,169 (GRCm39) M138K possibly damaging Het
Or7g25 T C 9: 19,160,039 (GRCm39) I219V probably benign Het
Oxct1 T A 15: 4,120,677 (GRCm39) F254I probably benign Het
Patj T A 4: 98,342,633 (GRCm39) S576T possibly damaging Het
Pdxdc1 A T 16: 13,690,363 (GRCm39) C202S probably benign Het
Pet100 A T 8: 3,672,381 (GRCm39) I19F probably damaging Het
Pik3r5 T C 11: 68,385,059 (GRCm39) Y655H probably damaging Het
Pkhd1 A G 1: 20,151,476 (GRCm39) S3807P probably damaging Het
Ptma A G 1: 86,454,649 (GRCm39) T8A probably damaging Het
Ptpn3 G T 4: 57,240,843 (GRCm39) N257K probably damaging Het
R3hdm1 C A 1: 128,164,409 (GRCm39) Q1108K probably benign Het
Rimbp2 T C 5: 128,857,406 (GRCm39) D815G probably damaging Het
Scn10a A G 9: 119,523,196 (GRCm39) F66L probably benign Het
Selenot T C 3: 58,484,296 (GRCm39) probably null Het
Serpinb8 A G 1: 107,526,705 (GRCm39) T82A probably benign Het
Serpini2 G A 3: 75,175,295 (GRCm39) probably benign Het
Slc1a6 G T 10: 78,631,816 (GRCm39) G214V probably benign Het
Slc5a6 C A 5: 31,195,444 (GRCm39) A425S probably damaging Het
Smo C A 6: 29,736,123 (GRCm39) N38K possibly damaging Het
Smtn A T 11: 3,470,811 (GRCm39) C909* probably null Het
Sntg2 C T 12: 30,308,040 (GRCm39) R215H probably benign Het
Tdrd7 A G 4: 46,005,358 (GRCm39) D388G possibly damaging Het
Ttn A T 2: 76,642,652 (GRCm39) F13294L probably damaging Het
Ube2j1 T G 4: 33,040,745 (GRCm39) F84V probably damaging Het
Ugt3a1 A G 15: 9,370,287 (GRCm39) Q477R probably damaging Het
Utf1 T C 7: 139,523,859 (GRCm39) S25P probably benign Het
Vmn1r184 A T 7: 25,966,413 (GRCm39) H53L probably damaging Het
Vmn2r13 A T 5: 109,339,860 (GRCm39) N38K possibly damaging Het
Vps13a C T 19: 16,699,775 (GRCm39) D756N probably damaging Het
Vwa1 G T 4: 155,857,695 (GRCm39) D34E probably damaging Het
Zfhx2 A C 14: 55,301,774 (GRCm39) L2070R probably damaging Het
Zfp984 A G 4: 147,840,362 (GRCm39) V163A probably benign Het
Zp3r A T 1: 130,511,208 (GRCm39) M325K probably benign Het
Other mutations in Stk4
AlleleSourceChrCoordTypePredicted EffectPPH Score
IGL00672:Stk4 APN 2 163,959,999 (GRCm39) missense probably benign 0.05
IGL01583:Stk4 APN 2 163,916,134 (GRCm39) start codon destroyed probably null 0.21
IGL01933:Stk4 APN 2 163,940,505 (GRCm39) unclassified probably benign
IGL02084:Stk4 APN 2 163,928,527 (GRCm39) missense probably benign 0.05
IGL02423:Stk4 APN 2 163,928,419 (GRCm39) missense probably benign 0.00
IGL02601:Stk4 APN 2 163,928,462 (GRCm39) missense probably damaging 1.00
IGL02712:Stk4 APN 2 163,938,817 (GRCm39) missense probably damaging 1.00
hallon UTSW 2 163,941,747 (GRCm39) critical splice donor site probably null
iwo_jima UTSW 2 163,930,879 (GRCm39) missense possibly damaging 0.94
ribeye UTSW 2 163,921,486 (GRCm39) missense probably damaging 1.00
Sergeant UTSW 2 163,941,632 (GRCm39) missense probably benign
stryker UTSW 2 163,925,608 (GRCm39) nonsense probably null
R0377:Stk4 UTSW 2 163,938,720 (GRCm39) missense probably damaging 1.00
R0607:Stk4 UTSW 2 163,940,462 (GRCm39) missense probably damaging 1.00
R1403:Stk4 UTSW 2 163,942,448 (GRCm39) missense probably benign 0.04
R1403:Stk4 UTSW 2 163,942,448 (GRCm39) missense probably benign 0.04
R1404:Stk4 UTSW 2 163,942,448 (GRCm39) missense probably benign 0.04
R1404:Stk4 UTSW 2 163,942,448 (GRCm39) missense probably benign 0.04
R1405:Stk4 UTSW 2 163,942,448 (GRCm39) missense probably benign 0.04
R1405:Stk4 UTSW 2 163,942,448 (GRCm39) missense probably benign 0.04
R1406:Stk4 UTSW 2 163,942,448 (GRCm39) missense probably benign 0.04
R1406:Stk4 UTSW 2 163,942,448 (GRCm39) missense probably benign 0.04
R1972:Stk4 UTSW 2 163,942,448 (GRCm39) missense probably benign 0.04
R1973:Stk4 UTSW 2 163,942,448 (GRCm39) missense probably benign 0.04
R1976:Stk4 UTSW 2 163,942,448 (GRCm39) missense probably benign 0.04
R2025:Stk4 UTSW 2 163,938,751 (GRCm39) missense probably damaging 1.00
R3155:Stk4 UTSW 2 163,993,663 (GRCm39) missense probably benign 0.01
R3732:Stk4 UTSW 2 163,930,828 (GRCm39) missense probably benign 0.05
R3732:Stk4 UTSW 2 163,930,828 (GRCm39) missense probably benign 0.05
R3733:Stk4 UTSW 2 163,930,828 (GRCm39) missense probably benign 0.05
R3734:Stk4 UTSW 2 163,930,828 (GRCm39) missense probably benign 0.05
R4288:Stk4 UTSW 2 163,941,632 (GRCm39) missense probably benign
R4296:Stk4 UTSW 2 163,959,904 (GRCm39) missense possibly damaging 0.69
R4360:Stk4 UTSW 2 163,930,879 (GRCm39) missense possibly damaging 0.94
R4829:Stk4 UTSW 2 163,941,747 (GRCm39) critical splice donor site probably null
R4954:Stk4 UTSW 2 163,993,602 (GRCm39) missense probably damaging 1.00
R4954:Stk4 UTSW 2 163,993,601 (GRCm39) missense possibly damaging 0.75
R5088:Stk4 UTSW 2 163,925,608 (GRCm39) nonsense probably null
R5188:Stk4 UTSW 2 163,930,828 (GRCm39) missense possibly damaging 0.85
R5283:Stk4 UTSW 2 163,952,199 (GRCm39) nonsense probably null
R5605:Stk4 UTSW 2 163,921,486 (GRCm39) missense probably damaging 1.00
R5694:Stk4 UTSW 2 163,942,484 (GRCm39) missense possibly damaging 0.87
R5711:Stk4 UTSW 2 163,941,674 (GRCm39) missense probably benign 0.20
R7453:Stk4 UTSW 2 163,928,522 (GRCm39) missense probably benign 0.01
R7698:Stk4 UTSW 2 163,925,663 (GRCm39) missense probably damaging 1.00
R7726:Stk4 UTSW 2 163,952,146 (GRCm39) start codon destroyed probably null
R8177:Stk4 UTSW 2 163,930,777 (GRCm39) missense probably damaging 0.99
R9076:Stk4 UTSW 2 163,959,985 (GRCm39) missense probably benign
R9378:Stk4 UTSW 2 163,952,136 (GRCm39) intron probably benign
Predicted Primers PCR Primer
(F):5'- TTTGTGCCCCAGGTTTGAAGAG -3'
(R):5'- TACTCAGCTGACCTCTGTGG -3'

Sequencing Primer
(F):5'- CAGTGGTGGCCTTTAATCCCAG -3'
(R):5'- TCTGTGGCAAGCGAGACCTAAG -3'
Posted On 2016-10-24