Mutagenetix > Incidental Mutation

Incidental Mutation 'R5567:Muc4'

436964

Institutional Source

Beutler Lab

Gene Symbol

Muc4

Ensembl Gene

ENSMUSG00000079620

Gene Name

mucin 4

Synonyms

4933405I11Rik

MMRRC Submission

043124-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.079) question?

Stock #

R5567 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

32555089-32602761 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to G at 32598066 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Glutamic Acid to Glycine at position 3237 (E3237G)

Ref Sequence

ENSEMBL: ENSMUSP00000093813 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000096106] [ENSMUST00000115116]

AlphaFold

no structure available at present

Predicted Effect

possibly damaging
Transcript: ENSMUST00000096106
AA Change: E3237G

PolyPhen 2 Score 0.719 (Sensitivity: 0.86; Specificity: 0.92)

SMART Domains

Protein: ENSMUSP00000093813
Gene: ENSMUSG00000079620
AA Change: E3237G

Domain	Start	End	E-Value	Type
signal peptide	1	28	N/A	INTRINSIC
low complexity region	37	65	N/A	INTRINSIC
low complexity region	86	111	N/A	INTRINSIC
internal_repeat_2	119	903	6.07e-127	PROSPERO
internal_repeat_1	164	987	3.47e-144	PROSPERO
internal_repeat_2	979	1875	6.07e-127	PROSPERO
internal_repeat_1	1193	2087	3.47e-144	PROSPERO
low complexity region	2090	2106	N/A	INTRINSIC
low complexity region	2111	2119	N/A	INTRINSIC
low complexity region	2186	2195	N/A	INTRINSIC
low complexity region	2230	2249	N/A	INTRINSIC
low complexity region	2324	2332	N/A	INTRINSIC
low complexity region	2344	2367	N/A	INTRINSIC
NIDO	2458	2615	8.33e-67	SMART
AMOP	2614	2726	1.29e-47	SMART
VWD	2729	2910	4.23e-26	SMART
EGF_like	3134	3166	3.23e1	SMART
EGF_like	3176	3212	3.5e1	SMART
low complexity region	3237	3251	N/A	INTRINSIC
EGF	3384	3421	1.4e0	SMART
transmembrane domain	3430	3452	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000115116

SMART Domains

Protein: ENSMUSP00000110769
Gene: ENSMUSG00000035638

Domain	Start	End	E-Value	Type
signal peptide	1	23	N/A	INTRINSIC
low complexity region	97	113	N/A	INTRINSIC
internal_repeat_1	114	146	2.16e-8	PROSPERO
internal_repeat_1	138	170	2.16e-8	PROSPERO
internal_repeat_2	144	161	4e-5	PROSPERO
low complexity region	171	204	N/A	INTRINSIC
low complexity region	210	227	N/A	INTRINSIC
internal_repeat_2	228	245	4e-5	PROSPERO
low complexity region	324	351	N/A	INTRINSIC
low complexity region	376	385	N/A	INTRINSIC
low complexity region	516	550	N/A	INTRINSIC
low complexity region	574	593	N/A	INTRINSIC
low complexity region	662	679	N/A	INTRINSIC

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000115119

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000131419

Predicted Effect

unknown
Transcript: ENSMUST00000135753
AA Change: E1167G

SMART Domains

Protein: ENSMUSP00000119154
Gene: ENSMUSG00000079620
AA Change: E1167G

Domain	Start	End	E-Value	Type
low complexity region	21	37	N/A	INTRINSIC
low complexity region	42	50	N/A	INTRINSIC
low complexity region	117	126	N/A	INTRINSIC
low complexity region	161	180	N/A	INTRINSIC
low complexity region	255	263	N/A	INTRINSIC
low complexity region	275	298	N/A	INTRINSIC
NIDO	389	546	8.33e-67	SMART
AMOP	545	657	1.29e-47	SMART
VWD	660	841	4.23e-26	SMART
EGF_like	1065	1097	3.23e1	SMART
EGF_like	1107	1143	3.5e1	SMART
low complexity region	1168	1182	N/A	INTRINSIC
EGF	1315	1352	1.4e0	SMART
transmembrane domain	1361	1383	N/A	INTRINSIC

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000142355

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000143297

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000149773

Coding Region Coverage

1x: 99.9%
3x: 99.7%
10x: 98.6%
20x: 96.0%

Validation Efficiency

MGI Phenotype

FUNCTION: The major constituents of mucus, the viscous secretion that covers epithelial surfaces such as those in the trachea, colon, and cervix, are highly glycosylated proteins called mucins. These glycoproteins play important roles in the protection of the epithelial cells and have been implicated in epithelial renewal and differentiation. This gene encodes an integral membrane glycoprotein found on the cell surface. A large 5' exon encodes at least 15 tandem repeats of 124-126 amino acids. [provided by RefSeq, Jul 2008]
PHENOTYPE: Mice homozygous for a knock-out allele exhibit resistance to DSS-treated colitis and colitis-associated colorectal cancer. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 84 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Aak1	C	T	6: 86,932,150 (GRCm39)	Q374*	probably null	Het
Adam19	A	G	11: 46,027,077 (GRCm39)	D570G	probably damaging	Het
Aqr	G	A	2: 113,979,451 (GRCm39)	T328M	probably damaging	Het
Bltp3b	T	A	10: 89,644,383 (GRCm39)	M29K	probably benign	Het
Boc	G	A	16: 44,313,187 (GRCm39)	T559I	probably damaging	Het
Cadps	A	T	14: 12,473,497 (GRCm38)	I876N	possibly damaging	Het
Cdh9	A	G	15: 16,855,930 (GRCm39)	T657A	probably damaging	Het
Cep104	T	C	4: 154,086,734 (GRCm39)	S794P	possibly damaging	Het
Cep72	T	C	13: 74,188,260 (GRCm39)	Q211R	probably benign	Het
Coq6	T	A	12: 84,415,413 (GRCm39)	D145E	probably benign	Het
Crym	T	C	7: 119,801,116 (GRCm39)	E11G	probably benign	Het
Csmd3	A	T	15: 47,508,864 (GRCm39)	M2907K	possibly damaging	Het
Cspg4	A	C	9: 56,793,932 (GRCm39)	I556L	probably benign	Het
Cwf19l2	T	A	9: 3,456,831 (GRCm39)	D721E	probably damaging	Het
Defb36	T	A	2: 152,454,503 (GRCm39)	V54E	probably damaging	Het
Dlg5	C	A	14: 24,242,981 (GRCm39)	E55*	probably null	Het
Dna2	G	T	10: 62,802,452 (GRCm39)	A857S	possibly damaging	Het
E330034G19Rik	A	G	14: 24,346,892 (GRCm39)	K60E	possibly damaging	Het
Eps8l2	G	A	7: 140,934,920 (GRCm39)	R76Q	possibly damaging	Het
Fbxl18	A	G	5: 142,881,022 (GRCm39)		probably benign	Het
Flnc	T	A	6: 29,444,044 (GRCm39)	C619*	probably null	Het
Ftcd	A	G	10: 76,423,967 (GRCm39)	K503E	probably benign	Het
Gbp2b	C	T	3: 142,317,126 (GRCm39)	A494V	possibly damaging	Het
Gfral	C	T	9: 76,115,900 (GRCm39)	A25T	probably benign	Het
Gins2	T	A	8: 121,315,684 (GRCm39)	D24V	possibly damaging	Het
Gm10985	T	C	3: 53,752,683 (GRCm39)	I22T	probably damaging	Het
Golga4	T	A	9: 118,387,251 (GRCm39)	S1458T	probably damaging	Het
Gpatch1	T	C	7: 35,006,640 (GRCm39)	K176R	probably damaging	Het
Gsto1	T	C	19: 47,846,338 (GRCm39)	W62R	probably damaging	Het
Gtf2h3	C	T	5: 124,722,360 (GRCm39)	T121I	probably benign	Het
Gxylt1	A	G	15: 93,152,180 (GRCm39)		probably null	Het
Hs6st1	C	T	1: 36,142,719 (GRCm39)	P218L	probably benign	Het
Itpr3	C	T	17: 27,322,880 (GRCm39)	T1119M	possibly damaging	Het
Kif5c	A	G	2: 49,620,211 (GRCm39)	D226G	possibly damaging	Het
Klk1b1	T	A	7: 43,620,593 (GRCm39)	S228T	probably damaging	Het
Krt1	A	G	15: 101,755,340 (GRCm39)	F473S	probably benign	Het
Large1	A	G	8: 73,564,081 (GRCm39)	S562P	possibly damaging	Het
Lpxn	T	A	19: 12,810,023 (GRCm39)	M265K	possibly damaging	Het
Lrriq1	G	A	10: 103,006,457 (GRCm39)	P1223S	possibly damaging	Het
Map3k9	A	G	12: 81,778,798 (GRCm39)	L505P	possibly damaging	Het
Morn2	A	G	17: 80,604,709 (GRCm39)	D128G	probably damaging	Het
Msl2	A	G	9: 100,978,936 (GRCm39)	K437E	possibly damaging	Het
Msr1	T	C	8: 40,064,760 (GRCm39)	I305V	probably benign	Het
Mxra8	T	C	4: 155,925,465 (GRCm39)	M58T	probably damaging	Het
Myo16	A	G	8: 10,372,676 (GRCm39)	D125G	probably damaging	Het
Myom2	A	G	8: 15,152,546 (GRCm39)	Q631R	probably benign	Het
Nlrp1b	T	A	11: 71,072,229 (GRCm39)	H538L	probably benign	Het
Nme3	T	A	17: 25,115,823 (GRCm39)		probably null	Het
Nop2	T	C	6: 125,110,726 (GRCm39)	S68P	probably benign	Het
Nploc4	C	T	11: 120,275,440 (GRCm39)	V499M	probably benign	Het
Nub1	A	G	5: 24,913,814 (GRCm39)	E565G	possibly damaging	Het
Or1e19	C	T	11: 73,316,272 (GRCm39)	C179Y	probably damaging	Het
Or6e1	A	G	14: 54,519,825 (GRCm39)	F176L	probably damaging	Het
Or7e168	A	G	9: 19,719,674 (GRCm39)	D20G	probably damaging	Het
Or9g3	G	A	2: 85,589,994 (GRCm39)	S242F	probably damaging	Het
Parl	A	G	16: 20,101,762 (GRCm39)	M90T	probably damaging	Het
Pelo	T	C	13: 115,226,152 (GRCm39)	I102V	probably benign	Het
Pigt	T	A	2: 164,343,482 (GRCm39)	Y319*	probably null	Het
Plb1	A	G	5: 32,521,543 (GRCm39)	T1465A	unknown	Het
Plxna4	T	C	6: 32,134,915 (GRCm39)	N1763D	possibly damaging	Het
Pmpca	A	G	2: 26,280,553 (GRCm39)	E133G	probably damaging	Het
Pus7l	G	A	15: 94,425,746 (GRCm39)	P552S	probably benign	Het
Qsox2	A	G	2: 26,115,230 (GRCm39)	M1T	probably null	Het
Ralb	C	T	1: 119,411,265 (GRCm39)	V25I	probably damaging	Het
Rbbp6	A	G	7: 122,601,057 (GRCm39)		probably benign	Het
Rnf43	A	G	11: 87,618,271 (GRCm39)	E187G	probably damaging	Het
Rpl31	C	T	1: 39,409,108 (GRCm39)	R41C	probably benign	Het
Scap	A	G	9: 110,206,712 (GRCm39)	S386G	probably damaging	Het
Skint10	T	C	4: 112,573,067 (GRCm39)	Y243C	probably damaging	Het
Stpg2	A	G	3: 139,125,547 (GRCm39)	T447A	probably benign	Het
Tas1r1	G	A	4: 152,122,782 (GRCm39)	A21V	probably damaging	Het
Tas2r122	C	T	6: 132,688,335 (GRCm39)	G186E	probably benign	Het
Tenm4	C	A	7: 96,545,416 (GRCm39)	Y2477*	probably null	Het
Them7	A	G	2: 105,209,153 (GRCm39)	T158A	probably benign	Het
Tmcc2	A	C	1: 132,285,543 (GRCm39)	S608A	probably benign	Het
Ttc41	T	C	10: 86,596,784 (GRCm39)		probably null	Het
Ttk	T	A	9: 83,744,588 (GRCm39)	N593K	possibly damaging	Het
Tulp1	A	G	17: 28,578,172 (GRCm39)	V289A	possibly damaging	Het
Unc93a2	T	C	17: 7,631,202 (GRCm39)	T456A	probably benign	Het
Usp34	A	C	11: 23,438,336 (GRCm39)	Q278P	probably damaging	Het
Vmn2r78	T	A	7: 86,570,737 (GRCm39)	D418E	probably benign	Het
Vmn2r90	C	T	17: 17,932,336 (GRCm39)	A81V	probably damaging	Het
Xpc	T	A	6: 91,475,117 (GRCm39)	N636I	probably damaging	Het
Zfp563	A	T	17: 33,308,431 (GRCm39)		probably benign	Het

Other mutations in Muc4

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00087:Muc4	APN	16	32,754,086 (GRCm38)	missense	probably benign	0.35
IGL00088:Muc4	APN	16	32,754,086 (GRCm38)	missense	probably benign	0.35
IGL00089:Muc4	APN	16	32,754,086 (GRCm38)	missense	probably benign	0.35
IGL00090:Muc4	APN	16	32,754,086 (GRCm38)	missense	probably benign	0.35
IGL00091:Muc4	APN	16	32,754,086 (GRCm38)	missense	probably benign	0.35
IGL00092:Muc4	APN	16	32,754,086 (GRCm38)	missense	probably benign	0.35
IGL00163:Muc4	APN	16	32,754,090 (GRCm38)	missense	probably benign	0.20
IGL00324:Muc4	APN	16	32,599,186 (GRCm39)	missense	probably benign	0.03
IGL00331:Muc4	APN	16	32,574,613 (GRCm39)	missense	probably benign	0.01
IGL00539:Muc4	APN	16	32,569,728 (GRCm39)	missense	possibly damaging	0.53
IGL00590:Muc4	APN	16	32,575,465 (GRCm39)	missense	probably benign	0.10
IGL00990:Muc4	APN	16	32,753,886 (GRCm38)	missense	probably benign	0.01
IGL00990:Muc4	APN	16	32,753,863 (GRCm38)	missense	probably benign	0.01
IGL00990:Muc4	APN	16	32,752,569 (GRCm38)	missense	probably benign	0.01
IGL00990:Muc4	APN	16	32,575,140 (GRCm39)	missense	probably benign	0.01
IGL00990:Muc4	APN	16	32,575,139 (GRCm39)	missense	probably benign	0.01
IGL00990:Muc4	APN	16	32,575,114 (GRCm39)	missense	probably benign	0.01
IGL00990:Muc4	APN	16	32,576,179 (GRCm39)	unclassified	probably benign
IGL00990:Muc4	APN	16	32,575,246 (GRCm39)	missense	possibly damaging	0.86
IGL00990:Muc4	APN	16	32,575,362 (GRCm39)	missense	probably benign	0.01
IGL01091:Muc4	APN	16	32,753,927 (GRCm38)	missense	probably benign	0.04
IGL01124:Muc4	APN	16	32,589,104 (GRCm39)	missense	possibly damaging	0.65
IGL01131:Muc4	APN	16	32,753,901 (GRCm38)	missense	possibly damaging	0.72
IGL01536:Muc4	APN	16	32,584,340 (GRCm39)	missense	possibly damaging	0.93
IGL01603:Muc4	APN	16	32,569,473 (GRCm39)	missense	probably benign	0.23
IGL01618:Muc4	APN	16	32,577,001 (GRCm39)	missense	unknown
IGL01625:Muc4	APN	16	32,575,918 (GRCm39)	unclassified	probably benign
IGL01626:Muc4	APN	16	32,555,220 (GRCm39)	missense	possibly damaging	0.48
IGL01653:Muc4	APN	16	32,581,722 (GRCm39)	splice site	probably null
IGL01682:Muc4	APN	16	32,754,086 (GRCm38)	missense	probably benign	0.35
IGL01870:Muc4	APN	16	32,574,624 (GRCm39)	missense	probably benign	0.01
IGL01966:Muc4	APN	16	32,570,244 (GRCm39)	missense	possibly damaging	0.84
IGL01973:Muc4	APN	16	32,575,383 (GRCm39)	missense	probably benign	0.01
IGL02089:Muc4	APN	16	32,570,131 (GRCm39)	missense	possibly damaging	0.83
IGL02152:Muc4	APN	16	32,598,023 (GRCm39)	splice site	probably benign
IGL02210:Muc4	APN	16	32,574,054 (GRCm39)	missense	probably benign	0.00
IGL02278:Muc4	APN	16	32,754,529 (GRCm38)	missense	probably benign	0.01
IGL02280:Muc4	APN	16	32,754,529 (GRCm38)	missense	probably benign	0.01
IGL02316:Muc4	APN	16	32,569,668 (GRCm39)	missense	possibly damaging	0.73
IGL02351:Muc4	APN	16	32,569,804 (GRCm39)	missense	possibly damaging	0.86
IGL02358:Muc4	APN	16	32,569,804 (GRCm39)	missense	possibly damaging	0.86
IGL02391:Muc4	APN	16	32,570,894 (GRCm39)	missense	probably benign	0.41
IGL02449:Muc4	APN	16	32,576,503 (GRCm39)	unclassified	probably benign
IGL02607:Muc4	APN	16	32,596,193 (GRCm39)	missense	possibly damaging	0.73
IGL02888:Muc4	APN	16	32,575,656 (GRCm39)	unclassified	probably benign
IGL02893:Muc4	APN	16	32,570,466 (GRCm39)	missense	possibly damaging	0.68
IGL02902:Muc4	APN	16	32,569,212 (GRCm39)	missense	possibly damaging	0.50
IGL03007:Muc4	APN	16	32,570,866 (GRCm39)	missense	possibly damaging	0.84
IGL03161:Muc4	APN	16	32,570,766 (GRCm39)	missense	possibly damaging	0.84
IGL03304:Muc4	APN	16	32,570,257 (GRCm39)	nonsense	probably null
IGL03335:Muc4	APN	16	32,574,449 (GRCm39)	missense	probably benign	0.01
IGL03411:Muc4	APN	16	32,575,436 (GRCm39)	missense	probably benign	0.01
multisplendored	UTSW	16	32,569,869 (GRCm39)	missense	possibly damaging	0.53
protean	UTSW	16	32,754,689 (GRCm38)	missense	probably benign	0.00
R0864_muc4_269	UTSW	16	32,570,820 (GRCm39)	missense	probably benign	0.01
3-1:Muc4	UTSW	16	32,590,768 (GRCm39)	missense	possibly damaging	0.86
3-1:Muc4	UTSW	16	32,580,625 (GRCm39)	splice site	probably benign
BB004:Muc4	UTSW	16	32,592,011 (GRCm39)	missense
BB014:Muc4	UTSW	16	32,592,011 (GRCm39)	missense
IGL02835:Muc4	UTSW	16	32,584,319 (GRCm39)	missense	probably benign	0.32
P0035:Muc4	UTSW	16	32,580,622 (GRCm39)	splice site	probably benign
PIT4131001:Muc4	UTSW	16	32,755,684 (GRCm38)	unclassified	probably benign
PIT4131001:Muc4	UTSW	16	32,755,699 (GRCm38)	unclassified	probably benign
PIT4131001:Muc4	UTSW	16	32,755,676 (GRCm38)	unclassified	probably benign
PIT4142001:Muc4	UTSW	16	32,755,684 (GRCm38)	unclassified	probably benign
PIT4142001:Muc4	UTSW	16	32,754,529 (GRCm38)	missense	probably benign	0.01
PIT4142001:Muc4	UTSW	16	32,755,676 (GRCm38)	unclassified	probably benign
PIT4366001:Muc4	UTSW	16	32,575,542 (GRCm39)	missense	unknown
PIT4531001:Muc4	UTSW	16	32,576,391 (GRCm39)	missense	unknown
R0119:Muc4	UTSW	16	32,569,013 (GRCm39)	critical splice acceptor site	probably benign
R0133:Muc4	UTSW	16	32,591,978 (GRCm39)	missense	possibly damaging	0.91
R0136:Muc4	UTSW	16	32,569,013 (GRCm39)	critical splice acceptor site	probably benign
R0243:Muc4	UTSW	16	32,586,120 (GRCm39)	missense	possibly damaging	0.53
R0277:Muc4	UTSW	16	32,755,690 (GRCm38)	unclassified	probably benign
R0299:Muc4	UTSW	16	32,569,013 (GRCm39)	critical splice acceptor site	probably benign
R0380:Muc4	UTSW	16	32,574,333 (GRCm39)	missense	probably benign	0.00
R0462:Muc4	UTSW	16	32,582,910 (GRCm39)	missense	possibly damaging	0.93
R0507:Muc4	UTSW	16	32,569,887 (GRCm39)	missense	probably benign	0.01
R0508:Muc4	UTSW	16	32,570,131 (GRCm39)	missense	possibly damaging	0.83
R0543:Muc4	UTSW	16	32,577,120 (GRCm39)	missense	unknown
R0578:Muc4	UTSW	16	32,755,690 (GRCm38)	unclassified	probably benign
R0617:Muc4	UTSW	16	32,570,925 (GRCm39)	missense	possibly damaging	0.83
R0656:Muc4	UTSW	16	32,570,488 (GRCm39)	missense	possibly damaging	0.91
R0726:Muc4	UTSW	16	32,590,201 (GRCm39)	missense	probably damaging	0.99
R0727:Muc4	UTSW	16	32,590,221 (GRCm39)	missense	probably benign	0.01
R0776:Muc4	UTSW	16	32,574,020 (GRCm39)	missense	probably benign	0.04
R0854:Muc4	UTSW	16	32,599,329 (GRCm39)	missense	possibly damaging	0.53
R0862:Muc4	UTSW	16	32,570,820 (GRCm39)	missense	probably benign	0.01
R0864:Muc4	UTSW	16	32,570,820 (GRCm39)	missense	probably benign	0.01
R0926:Muc4	UTSW	16	32,576,570 (GRCm39)	unclassified	probably benign
R0990:Muc4	UTSW	16	32,752,722 (GRCm38)	missense	probably benign	0.00
R1127:Muc4	UTSW	16	32,569,343 (GRCm39)	missense	possibly damaging	0.92
R1203:Muc4	UTSW	16	32,754,529 (GRCm38)	missense	probably benign	0.01
R1433:Muc4	UTSW	16	32,574,448 (GRCm39)	missense	probably benign	0.00
R1466:Muc4	UTSW	16	32,574,886 (GRCm39)	missense	probably benign	0.04
R1466:Muc4	UTSW	16	32,574,886 (GRCm39)	missense	probably benign	0.04
R1506:Muc4	UTSW	16	32,574,033 (GRCm39)	missense	possibly damaging	0.59
R1518:Muc4	UTSW	16	32,569,167 (GRCm39)	missense	possibly damaging	0.84
R1544:Muc4	UTSW	16	32,753,919 (GRCm38)	missense	probably benign	0.10
R1584:Muc4	UTSW	16	32,574,886 (GRCm39)	missense	probably benign	0.04
R1593:Muc4	UTSW	16	32,754,686 (GRCm38)	missense	probably benign	0.00
R1601:Muc4	UTSW	16	32,575,875 (GRCm39)	unclassified	probably benign
R1611:Muc4	UTSW	16	32,569,804 (GRCm39)	missense	possibly damaging	0.86
R1673:Muc4	UTSW	16	32,577,276 (GRCm39)	missense	probably benign	0.11
R1717:Muc4	UTSW	16	32,753,405 (GRCm38)	missense	possibly damaging	0.53
R1822:Muc4	UTSW	16	32,753,919 (GRCm38)	missense	probably benign	0.10
R1824:Muc4	UTSW	16	32,576,307 (GRCm39)	unclassified	probably benign
R1839:Muc4	UTSW	16	32,753,919 (GRCm38)	missense	probably benign	0.10
R1846:Muc4	UTSW	16	32,752,369 (GRCm38)	missense	probably benign	0.01
R1864:Muc4	UTSW	16	32,576,625 (GRCm39)	unclassified	probably benign
R1868:Muc4	UTSW	16	32,576,715 (GRCm39)	missense	unknown
R1928:Muc4	UTSW	16	32,569,350 (GRCm39)	missense	probably damaging	0.99
R1942:Muc4	UTSW	16	32,569,460 (GRCm39)	missense	probably damaging	0.99
R2017:Muc4	UTSW	16	32,570,121 (GRCm39)	missense	possibly damaging	0.92
R2023:Muc4	UTSW	16	32,574,054 (GRCm39)	missense	probably benign	0.00
R2081:Muc4	UTSW	16	32,574,020 (GRCm39)	missense	probably benign	0.04
R2088:Muc4	UTSW	16	32,576,783 (GRCm39)	missense	unknown
R2121:Muc4	UTSW	16	32,580,612 (GRCm39)	missense	unknown
R2139:Muc4	UTSW	16	32,581,599 (GRCm39)	missense	unknown
R2158:Muc4	UTSW	16	32,754,563 (GRCm38)	missense	probably benign	0.10
R2165:Muc4	UTSW	16	32,569,294 (GRCm39)	missense	probably damaging	0.96
R2210:Muc4	UTSW	16	32,755,176 (GRCm38)	frame shift	probably null
R2225:Muc4	UTSW	16	32,587,316 (GRCm39)	missense	possibly damaging	0.73
R2225:Muc4	UTSW	16	32,576,265 (GRCm39)	unclassified	probably benign
R2269:Muc4	UTSW	16	32,754,529 (GRCm38)	missense	probably benign	0.01
R2679:Muc4	UTSW	16	32,577,846 (GRCm39)	missense	unknown
R3703:Muc4	UTSW	16	32,753,919 (GRCm38)	missense	probably benign	0.10
R3816:Muc4	UTSW	16	32,754,529 (GRCm38)	missense	probably benign	0.01
R3909:Muc4	UTSW	16	32,753,919 (GRCm38)	missense	probably benign	0.10
R4014:Muc4	UTSW	16	32,575,647 (GRCm39)	unclassified	probably benign
R4065:Muc4	UTSW	16	32,569,869 (GRCm39)	missense	possibly damaging	0.53
R4066:Muc4	UTSW	16	32,569,869 (GRCm39)	missense	possibly damaging	0.53
R4067:Muc4	UTSW	16	32,569,869 (GRCm39)	missense	possibly damaging	0.53
R4245:Muc4	UTSW	16	32,753,802 (GRCm38)	missense	probably benign	0.04
R4249:Muc4	UTSW	16	32,576,200 (GRCm39)	unclassified	probably benign
R4344:Muc4	UTSW	16	32,590,666 (GRCm39)	missense	possibly damaging	0.53
R4388:Muc4	UTSW	16	32,753,802 (GRCm38)	missense	probably benign	0.04
R4393:Muc4	UTSW	16	32,754,529 (GRCm38)	missense	probably benign	0.01
R4482:Muc4	UTSW	16	32,577,075 (GRCm39)	missense	unknown
R4523:Muc4	UTSW	16	32,555,154 (GRCm39)	utr 5 prime	probably benign
R4527:Muc4	UTSW	16	32,576,217 (GRCm39)	unclassified	probably benign
R4572:Muc4	UTSW	16	32,753,802 (GRCm38)	missense	probably benign	0.04
R4587:Muc4	UTSW	16	32,753,919 (GRCm38)	missense	probably benign	0.10
R4614:Muc4	UTSW	16	32,577,432 (GRCm39)	missense	probably benign	0.03
R4635:Muc4	UTSW	16	32,753,802 (GRCm38)	missense	probably benign	0.04
R4661:Muc4	UTSW	16	32,589,651 (GRCm39)	missense	possibly damaging	0.71
R4701:Muc4	UTSW	16	32,576,220 (GRCm39)	unclassified	probably benign
R4730:Muc4	UTSW	16	32,570,032 (GRCm39)	missense	possibly damaging	0.86
R4740:Muc4	UTSW	16	32,596,277 (GRCm39)	missense	possibly damaging	0.91
R4762:Muc4	UTSW	16	32,574,916 (GRCm39)	unclassified	probably benign
R4818:Muc4	UTSW	16	32,753,919 (GRCm38)	missense	probably benign	0.10
R4821:Muc4	UTSW	16	32,753,802 (GRCm38)	missense	probably benign	0.04
R4825:Muc4	UTSW	16	32,570,565 (GRCm39)	missense	probably benign	0.41
R4830:Muc4	UTSW	16	32,753,919 (GRCm38)	missense	probably benign	0.10
R4860:Muc4	UTSW	16	32,754,625 (GRCm38)	missense	probably benign	0.18
R4860:Muc4	UTSW	16	32,754,616 (GRCm38)	missense	probably benign	0.35
R4869:Muc4	UTSW	16	32,754,836 (GRCm38)	unclassified	probably benign
R4934:Muc4	UTSW	16	32,576,472 (GRCm39)	unclassified	probably benign
R4959:Muc4	UTSW	16	32,575,437 (GRCm39)	missense	possibly damaging	0.73
R4969:Muc4	UTSW	16	32,754,572 (GRCm38)	missense	probably benign	0.01
R4995:Muc4	UTSW	16	32,575,332 (GRCm39)	missense	probably benign	0.01
R4995:Muc4	UTSW	16	32,754,214 (GRCm38)	missense	probably benign	0.01
R4999:Muc4	UTSW	16	32,576,670 (GRCm39)	unclassified	probably benign
R5073:Muc4	UTSW	16	32,754,529 (GRCm38)	missense	probably benign	0.01
R5075:Muc4	UTSW	16	32,754,794 (GRCm38)	unclassified	probably benign
R5152:Muc4	UTSW	16	32,577,432 (GRCm39)	nonsense	probably null
R5161:Muc4	UTSW	16	32,582,895 (GRCm39)	missense	probably damaging	0.98
R5174:Muc4	UTSW	16	32,570,556 (GRCm39)	missense	possibly damaging	0.84
R5268:Muc4	UTSW	16	32,570,484 (GRCm39)	missense	possibly damaging	0.83
R5447:Muc4	UTSW	16	32,753,919 (GRCm38)	missense	probably benign	0.10
R5474:Muc4	UTSW	16	32,581,635 (GRCm39)	missense	unknown
R5570:Muc4	UTSW	16	32,598,066 (GRCm39)	missense	possibly damaging	0.72
R5618:Muc4	UTSW	16	32,754,253 (GRCm38)	missense	probably benign	0.01
R5665:Muc4	UTSW	16	32,569,600 (GRCm39)	missense	probably benign	0.33
R5667:Muc4	UTSW	16	32,575,011 (GRCm39)	missense	probably benign	0.01
R5671:Muc4	UTSW	16	32,575,011 (GRCm39)	missense	probably benign	0.01
R5693:Muc4	UTSW	16	32,597,181 (GRCm39)	missense	possibly damaging	0.53
R5703:Muc4	UTSW	16	32,555,059 (GRCm39)	nonsense	probably null
R5708:Muc4	UTSW	16	32,575,515 (GRCm39)	unclassified	probably benign
R5715:Muc4	UTSW	16	32,570,734 (GRCm39)	missense	possibly damaging	0.92
R5849:Muc4	UTSW	16	32,595,213 (GRCm39)	missense	possibly damaging	0.53
R5873:Muc4	UTSW	16	32,570,113 (GRCm39)	missense	possibly damaging	0.61
R5930:Muc4	UTSW	16	32,570,523 (GRCm39)	missense	probably benign	0.41
R5933:Muc4	UTSW	16	32,574,480 (GRCm39)	missense	probably benign	0.01
R5966:Muc4	UTSW	16	32,576,652 (GRCm39)	unclassified	probably benign
R6062:Muc4	UTSW	16	32,579,682 (GRCm39)	missense	unknown
R6067:Muc4	UTSW	16	32,754,529 (GRCm38)	missense	probably benign	0.01
R6067:Muc4	UTSW	16	32,575,621 (GRCm39)	unclassified	probably benign
R6078:Muc4	UTSW	16	32,575,621 (GRCm39)	unclassified	probably benign
R6079:Muc4	UTSW	16	32,575,621 (GRCm39)	unclassified	probably benign
R6112:Muc4	UTSW	16	32,596,157 (GRCm39)	missense	possibly damaging	0.86
R6120:Muc4	UTSW	16	32,577,169 (GRCm39)	missense	unknown
R6144:Muc4	UTSW	16	32,587,298 (GRCm39)	missense	possibly damaging	0.53
R6148:Muc4	UTSW	16	32,753,802 (GRCm38)	missense	probably benign	0.04
R6173:Muc4	UTSW	16	32,554,958 (GRCm39)	start gained	probably benign
R6268:Muc4	UTSW	16	32,589,141 (GRCm39)	missense	probably damaging	0.99
R6299:Muc4	UTSW	16	32,570,853 (GRCm39)	missense	possibly damaging	0.48
R6307:Muc4	UTSW	16	32,575,237 (GRCm39)	missense	possibly damaging	0.56
R6354:Muc4	UTSW	16	32,575,476 (GRCm39)	missense	probably benign	0.19
R6361:Muc4	UTSW	16	32,587,725 (GRCm39)	missense	probably benign	0.32
R6375:Muc4	UTSW	16	32,555,061 (GRCm39)	utr 5 prime	probably benign
R6378:Muc4	UTSW	16	32,599,320 (GRCm39)	missense	probably benign	0.33
R6418:Muc4	UTSW	16	32,570,607 (GRCm39)	missense	possibly damaging	0.68
R6458:Muc4	UTSW	16	32,579,694 (GRCm39)	critical splice donor site	probably null
R6527:Muc4	UTSW	16	32,753,433 (GRCm38)	missense	probably benign	0.01
R6616:Muc4	UTSW	16	32,602,378 (GRCm39)	missense	possibly damaging	0.93
R6636:Muc4	UTSW	16	32,575,255 (GRCm39)	missense	probably benign	0.01
R6637:Muc4	UTSW	16	32,575,255 (GRCm39)	missense	probably benign	0.01
R6915:Muc4	UTSW	16	32,587,312 (GRCm39)	missense	probably benign	0.18
R6947:Muc4	UTSW	16	32,596,177 (GRCm39)	missense	possibly damaging	0.91
R6976:Muc4	UTSW	16	32,582,892 (GRCm39)	missense	possibly damaging	0.92
R6985:Muc4	UTSW	16	32,570,817 (GRCm39)	missense	probably benign	0.00
R7020:Muc4	UTSW	16	32,570,628 (GRCm39)	nonsense	probably null
R7033:Muc4	UTSW	16	32,576,698 (GRCm39)	unclassified	probably benign
R7098:Muc4	UTSW	16	32,577,465 (GRCm39)	missense
R7123:Muc4	UTSW	16	32,569,509 (GRCm39)	missense	possibly damaging	0.83
R7173:Muc4	UTSW	16	32,582,862 (GRCm39)	missense	probably damaging	0.97
R7178:Muc4	UTSW	16	32,752,788 (GRCm38)	missense	unknown
R7294:Muc4	UTSW	16	32,576,835 (GRCm39)	missense	possibly damaging	0.53
R7318:Muc4	UTSW	16	32,575,710 (GRCm39)	missense	unknown
R7361:Muc4	UTSW	16	32,754,670 (GRCm38)	missense	probably benign	0.18
R7380:Muc4	UTSW	16	32,575,740 (GRCm39)	missense	unknown
R7381:Muc4	UTSW	16	32,601,285 (GRCm39)	missense
R7411:Muc4	UTSW	16	32,570,140 (GRCm39)	missense	probably benign	0.12
R7422:Muc4	UTSW	16	32,754,689 (GRCm38)	missense	probably benign	0.00
R7482:Muc4	UTSW	16	32,587,324 (GRCm39)	missense
R7539:Muc4	UTSW	16	32,576,770 (GRCm39)	missense
R7544:Muc4	UTSW	16	32,555,016 (GRCm39)	start codon destroyed	probably null
R7574:Muc4	UTSW	16	32,753,411 (GRCm38)	missense	probably benign	0.18
R7576:Muc4	UTSW	16	32,754,500 (GRCm38)	missense	probably benign	0.10
R7585:Muc4	UTSW	16	32,586,076 (GRCm39)	missense
R7596:Muc4	UTSW	16	32,575,221 (GRCm39)	missense	probably benign	0.01
R7597:Muc4	UTSW	16	32,575,221 (GRCm39)	missense	probably benign	0.01
R7602:Muc4	UTSW	16	32,575,221 (GRCm39)	missense	probably benign	0.01
R7616:Muc4	UTSW	16	32,574,161 (GRCm39)	nonsense	probably null
R7639:Muc4	UTSW	16	32,575,221 (GRCm39)	missense	probably benign	0.01
R7640:Muc4	UTSW	16	32,580,479 (GRCm39)	missense
R7651:Muc4	UTSW	16	32,576,949 (GRCm39)	missense
R7688:Muc4	UTSW	16	32,570,278 (GRCm39)	missense	possibly damaging	0.68
R7689:Muc4	UTSW	16	32,574,439 (GRCm39)	missense	probably benign	0.10
R7752:Muc4	UTSW	16	32,589,108 (GRCm39)	missense
R7763:Muc4	UTSW	16	32,574,739 (GRCm39)	missense	probably benign	0.10
R7768:Muc4	UTSW	16	32,576,568 (GRCm39)	missense	unknown
R7787:Muc4	UTSW	16	32,575,221 (GRCm39)	missense	probably benign	0.01
R7789:Muc4	UTSW	16	32,575,221 (GRCm39)	missense	probably benign	0.01
R7838:Muc4	UTSW	16	32,752,558 (GRCm38)	nonsense	probably null
R7871:Muc4	UTSW	16	32,754,935 (GRCm38)	missense	unknown
R7921:Muc4	UTSW	16	32,570,139 (GRCm39)	missense	possibly damaging	0.68
R7927:Muc4	UTSW	16	32,592,011 (GRCm39)	missense
R7930:Muc4	UTSW	16	32,575,369 (GRCm39)	unclassified	probably benign
R8000:Muc4	UTSW	16	32,575,221 (GRCm39)	missense	probably benign	0.01
R8062:Muc4	UTSW	16	32,577,123 (GRCm39)	missense
R8096:Muc4	UTSW	16	32,575,764 (GRCm39)	missense	unknown
R8115:Muc4	UTSW	16	32,575,678 (GRCm39)	missense	unknown
R8162:Muc4	UTSW	16	32,569,197 (GRCm39)	missense	unknown
R8220:Muc4	UTSW	16	32,575,701 (GRCm39)	missense	unknown
R8260:Muc4	UTSW	16	32,575,367 (GRCm39)	unclassified	probably benign
R8290:Muc4	UTSW	16	32,575,434 (GRCm39)	missense	probably benign	0.01
R8299:Muc4	UTSW	16	32,576,271 (GRCm39)	missense	unknown
R8313:Muc4	UTSW	16	32,753,423 (GRCm38)	missense	probably benign	0.04
R8356:Muc4	UTSW	16	32,575,367 (GRCm39)	unclassified	probably benign
R8463:Muc4	UTSW	16	32,574,201 (GRCm39)	missense	probably benign	0.00
R8479:Muc4	UTSW	16	32,574,799 (GRCm39)	missense	possibly damaging	0.59
R8480:Muc4	UTSW	16	32,574,421 (GRCm39)	missense	probably benign	0.20
R8510:Muc4	UTSW	16	32,575,367 (GRCm39)	unclassified	probably benign
R8515:Muc4	UTSW	16	32,575,629 (GRCm39)	missense	unknown
R8559:Muc4	UTSW	16	32,754,715 (GRCm38)	missense	unknown
R8685:Muc4	UTSW	16	32,575,221 (GRCm39)	missense	probably benign	0.01
R8687:Muc4	UTSW	16	32,575,221 (GRCm39)	missense	probably benign	0.01
R8728:Muc4	UTSW	16	32,754,952 (GRCm38)	missense	unknown
R8754:Muc4	UTSW	16	32,602,356 (GRCm39)	missense
R8845:Muc4	UTSW	16	32,576,889 (GRCm39)	missense	possibly damaging	0.86
R8852:Muc4	UTSW	16	32,569,461 (GRCm39)	missense	possibly damaging	0.66
R8863:Muc4	UTSW	16	32,570,280 (GRCm39)	missense	possibly damaging	0.84
R8896:Muc4	UTSW	16	32,754,673 (GRCm38)	missense	probably benign	0.01
R8929:Muc4	UTSW	16	32,754,017 (GRCm38)	missense	possibly damaging	0.53
R8929:Muc4	UTSW	16	32,575,285 (GRCm39)	missense	probably benign	0.00
R8990:Muc4	UTSW	16	32,574,009 (GRCm39)	missense	probably benign	0.01
R9018:Muc4	UTSW	16	32,582,910 (GRCm39)	missense
R9034:Muc4	UTSW	16	32,575,367 (GRCm39)	unclassified	probably benign
R9064:Muc4	UTSW	16	32,754,397 (GRCm38)	missense	possibly damaging	0.53
R9068:Muc4	UTSW	16	32,575,367 (GRCm39)	unclassified	probably benign
R9086:Muc4	UTSW	16	32,577,842 (GRCm39)	missense
R9151:Muc4	UTSW	16	32,752,617 (GRCm38)	missense	probably benign	0.01
R9187:Muc4	UTSW	16	32,589,102 (GRCm39)	missense
R9336:Muc4	UTSW	16	32,569,756 (GRCm39)	missense	possibly damaging	0.86
R9343:Muc4	UTSW	16	32,569,971 (GRCm39)	missense	possibly damaging	0.53
R9363:Muc4	UTSW	16	32,576,992 (GRCm39)	missense
R9429:Muc4	UTSW	16	32,576,098 (GRCm39)	missense	unknown
R9570:Muc4	UTSW	16	32,569,695 (GRCm39)	missense	possibly damaging	0.53
R9609:Muc4	UTSW	16	32,577,234 (GRCm39)	missense
R9696:Muc4	UTSW	16	32,574,712 (GRCm39)	missense	probably benign	0.00
R9709:Muc4	UTSW	16	32,590,644 (GRCm39)	missense
R9743:Muc4	UTSW	16	32,601,194 (GRCm39)	missense
R9747:Muc4	UTSW	16	32,754,698 (GRCm38)	missense	probably benign	0.01
RF014:Muc4	UTSW	16	32,570,676 (GRCm39)	missense	probably damaging	0.98
U15987:Muc4	UTSW	16	32,754,529 (GRCm38)	missense	probably benign	0.01
U15987:Muc4	UTSW	16	32,575,621 (GRCm39)	unclassified	probably benign
V5622:Muc4	UTSW	16	32,570,643 (GRCm39)	missense	probably benign	0.00
X0018:Muc4	UTSW	16	32,575,855 (GRCm39)	unclassified	probably benign
X0028:Muc4	UTSW	16	32,579,693 (GRCm39)	critical splice donor site	probably null
Z1088:Muc4	UTSW	16	32,576,450 (GRCm39)	unclassified	probably benign
Z1176:Muc4	UTSW	16	32,589,104 (GRCm39)	missense	possibly damaging	0.65
Z1177:Muc4	UTSW	16	32,587,767 (GRCm39)	nonsense	probably null
Z1177:Muc4	UTSW	16	32,595,236 (GRCm39)	missense
Z1177:Muc4	UTSW	16	32,587,768 (GRCm39)	missense

Predicted Primers

PCR Primer
(F):5'- TCATCTGCAGAGGACAGACG -3'
(R):5'- ATCCTGAAGCTTCCTGGCATC -3'

Sequencing Primer
(F):5'- TCAATAAGACGCTGTTGGCC -3'
(R):5'- TGAAGCTTCCTGGCATCCTAGG -3'

Posted On

2016-10-24