Incidental Mutation 'R5577:Prdx6'
ID 437244
Institutional Source Beutler Lab
Gene Symbol Prdx6
Ensembl Gene ENSMUSG00000026701
Gene Name peroxiredoxin 6
Synonyms Ltw4, aiPLA2, 9430088D19Rik, acidic calcium-independent phospholipase A2, Ltw-4, CP-3, Aop2, 1-cysPrx, GPx, 1-Cys Prx, CC26, Brp-12, Lvtw-4, NSGP
MMRRC Submission 043132-MU
Accession Numbers
Essential gene? Non essential (E-score: 0.000) question?
Stock # R5577 (G1)
Quality Score 225
Status Not validated
Chromosome 1
Chromosomal Location 161067682-161078780 bp(-) (GRCm39)
Type of Mutation missense
DNA Base Change (assembly) A to G at 161071255 bp (GRCm39)
Zygosity Heterozygous
Amino Acid Change Serine to Proline at position 146 (S146P)
Ref Sequence ENSEMBL: ENSMUSP00000071636 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000051925] [ENSMUST00000071718] [ENSMUST00000192639]
AlphaFold no structure available at present
Predicted Effect probably damaging
Transcript: ENSMUST00000051925
AA Change: S122P

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)
SMART Domains Protein: ENSMUSP00000050703
Gene: ENSMUSG00000026701
AA Change: S122P

DomainStartEndE-ValueType
Pfam:AhpC-TSA 3 122 1.8e-25 PFAM
Pfam:Redoxin 5 139 4.8e-9 PFAM
Pfam:1-cysPrx_C 142 181 6.1e-15 PFAM
Predicted Effect probably damaging
Transcript: ENSMUST00000071718
AA Change: S146P

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)
SMART Domains Protein: ENSMUSP00000071636
Gene: ENSMUSG00000026701
AA Change: S146P

DomainStartEndE-ValueType
Pfam:Redoxin 6 162 1.3e-11 PFAM
Pfam:AhpC-TSA 7 146 3.4e-30 PFAM
Pfam:1-cysPrx_C 166 205 6.2e-15 PFAM
Predicted Effect noncoding transcript
Transcript: ENSMUST00000139387
Predicted Effect noncoding transcript
Transcript: ENSMUST00000155135
Predicted Effect noncoding transcript
Transcript: ENSMUST00000156318
Predicted Effect noncoding transcript
Transcript: ENSMUST00000156678
Predicted Effect probably benign
Transcript: ENSMUST00000192639
SMART Domains Protein: ENSMUSP00000142093
Gene: ENSMUSG00000026701

DomainStartEndE-ValueType
PDB:1PRX|B 1 43 6e-16 PDB
SCOP:d1prxa_ 5 39 9e-6 SMART
Predicted Effect noncoding transcript
Transcript: ENSMUST00000194613
Coding Region Coverage
  • 1x: 99.9%
  • 3x: 99.7%
  • 10x: 98.6%
  • 20x: 96.2%
Validation Efficiency
MGI Phenotype FUNCTION: This gene encodes a member of the peroxiredoxin family of peroxidases. The encoded protein is a bifunctional enzyme that has glutathione peroxidase and phospholipase activities. This protein is an antioxidant that reduces peroxidized membrane phospholipids and plays an important role in phospholipid homeostasis based on its ability to generate lysophospholipid substrate for the remodeling pathway of phospholipid synthesis. Mice lacking this gene are sensitive to oxidant stress, have altered lung phospholipid metabolism and susceptible to skin tumorigenesis. Alternate splicing of this gene results in multiple transcript variants. A pseudogene of this gene is found on chromosome 4. [provided by RefSeq, Dec 2014]
PHENOTYPE: Mice homozygous for disruptions of this gene show no macroscopic or microscopic abnormalities. However, they have an increased susceptibility to oxidative stress. [provided by MGI curators]
Allele List at MGI
Other mutations in this stock
Total: 36 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
9130023H24Rik A C 7: 127,835,826 (GRCm39) Y256D probably damaging Het
Acr C A 15: 89,458,441 (GRCm39) T374K probably benign Het
Akt2 G A 7: 27,335,731 (GRCm39) G335R probably damaging Het
Ccn3 T A 15: 54,615,897 (GRCm39) I354N possibly damaging Het
Cd177 A T 7: 24,444,562 (GRCm39) F673Y probably damaging Het
Clmn G T 12: 104,743,329 (GRCm39) S879R probably damaging Het
Csrp3 T C 7: 48,489,225 (GRCm39) H19R possibly damaging Het
Dmkn T C 7: 30,463,971 (GRCm39) S137P probably damaging Het
Eno1 C A 4: 150,331,067 (GRCm39) Y236* probably null Het
Enpp7 T A 11: 118,882,953 (GRCm39) N342K probably benign Het
Fancm T A 12: 65,177,185 (GRCm39) probably benign Het
Fshr A T 17: 89,293,351 (GRCm39) D442E probably benign Het
Gm3898 T A 9: 43,741,362 (GRCm39) noncoding transcript Het
Hdac7 A T 15: 97,709,336 (GRCm39) S43T probably benign Het
Herc1 T C 9: 66,389,263 (GRCm39) C3927R probably damaging Het
Klc4 G T 17: 46,946,355 (GRCm39) A490D probably damaging Het
Lcn9 T A 2: 25,713,663 (GRCm39) I63N probably damaging Het
Lgalsl G A 11: 20,779,316 (GRCm39) Q110* probably null Het
Lrp1b A T 2: 40,765,135 (GRCm39) M2783K possibly damaging Het
Lrrk2 A T 15: 91,649,948 (GRCm39) Y1695F probably damaging Het
Myo1e G A 9: 70,277,753 (GRCm39) E817K probably benign Het
Nav3 A T 10: 109,605,264 (GRCm39) D936E probably damaging Het
Necab3 T C 2: 154,387,076 (GRCm39) probably null Het
Nlrp1a T A 11: 70,990,400 (GRCm39) I951F probably damaging Het
Or5v1 T A 17: 37,810,493 (GRCm39) I317K probably benign Het
Or8b52 A G 9: 38,576,297 (GRCm39) I281T possibly damaging Het
Ppp2r5d T C 17: 46,998,901 (GRCm39) S54G probably benign Het
Sec24a A T 11: 51,625,448 (GRCm39) H258Q probably benign Het
Sec31a T C 5: 100,550,133 (GRCm39) T194A possibly damaging Het
Sqstm1 T C 11: 50,098,266 (GRCm39) I167V probably benign Het
Tas1r3 T C 4: 155,946,522 (GRCm39) E361G probably benign Het
Tlr1 T A 5: 65,083,428 (GRCm39) Q383L possibly damaging Het
Trappc8 G C 18: 20,969,836 (GRCm39) Y1051* probably null Het
Vmn2r2 T A 3: 64,024,416 (GRCm39) M722L probably benign Het
Vmn2r43 T C 7: 8,247,811 (GRCm39) H784R probably damaging Het
Zfp534 T G 4: 147,759,173 (GRCm39) K499Q probably damaging Het
Other mutations in Prdx6
AlleleSourceChrCoordTypePredicted EffectPPH Score
R0540:Prdx6 UTSW 1 161,078,673 (GRCm39) missense probably damaging 1.00
R1479:Prdx6 UTSW 1 161,071,833 (GRCm39) missense probably damaging 0.99
R3700:Prdx6 UTSW 1 161,074,858 (GRCm39) missense probably damaging 1.00
R4930:Prdx6 UTSW 1 161,069,263 (GRCm39) utr 3 prime probably benign
R5412:Prdx6 UTSW 1 161,071,860 (GRCm39) missense probably damaging 0.99
R6842:Prdx6 UTSW 1 161,074,940 (GRCm39) missense probably damaging 1.00
R7229:Prdx6 UTSW 1 161,074,867 (GRCm39) missense probably damaging 1.00
R7450:Prdx6 UTSW 1 161,069,386 (GRCm39) missense probably benign
R7860:Prdx6 UTSW 1 161,069,428 (GRCm39) splice site probably null
R8379:Prdx6 UTSW 1 161,078,660 (GRCm39) missense probably benign 0.01
R8944:Prdx6 UTSW 1 161,069,432 (GRCm39) splice site probably benign
R9039:Prdx6 UTSW 1 161,078,619 (GRCm39) missense probably damaging 1.00
Predicted Primers PCR Primer
(F):5'- GGGACACTCTACTGGGACAATAG -3'
(R):5'- AATAAACTCCATTTTCCTCTGCCAG -3'

Sequencing Primer
(F):5'- ACATCCTGAAAGTGGCCATG -3'
(R):5'- AGTTCTGTTTTCTTCCCTGGACTAAG -3'
Posted On 2016-10-26