Mutagenetix > Incidental Mutation

Incidental Mutation 'R5577:Trappc8'

437279

Institutional Source

Beutler Lab

Gene Symbol

Trappc8

Ensembl Gene

ENSMUSG00000033382

Gene Name

trafficking protein particle complex 8

Synonyms

5033403J15Rik, D030074E01Rik, Trs85

MMRRC Submission

043132-MU

Accession Numbers

Essential gene?

Probably essential (E-score: 0.950) question?

Stock #

R5577 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

20950280-21029150 bp(-) (GRCm39)

Type of Mutation

nonsense

DNA Base Change (assembly)

G to C at 20969836 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Tyrosine to Stop codon at position 1051 (Y1051*)

Ref Sequence

ENSEMBL: ENSMUSP00000153183 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000025177] [ENSMUST00000225661]

AlphaFold

A0A286YCX6

Predicted Effect

probably null
Transcript: ENSMUST00000025177
AA Change: Y1052*

SMART Domains

Protein: ENSMUSP00000025177
Gene: ENSMUSG00000033382
AA Change: Y1052*

Domain	Start	End	E-Value	Type
Pfam:TRAPPC-Trs85	157	604	1e-167	PFAM
low complexity region	769	777	N/A	INTRINSIC

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000225502

Predicted Effect

probably null
Transcript: ENSMUST00000225661
AA Change: Y1051*

Coding Region Coverage

1x: 99.9%
3x: 99.7%
10x: 98.6%
20x: 96.2%

Validation Efficiency

Allele List at MGI

All alleles(11) : Gene trapped(11)

Other mutations in this stock

Total: 36 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
9130023H24Rik	A	C	7: 127,835,826 (GRCm39)	Y256D	probably damaging	Het
Acr	C	A	15: 89,458,441 (GRCm39)	T374K	probably benign	Het
Akt2	G	A	7: 27,335,731 (GRCm39)	G335R	probably damaging	Het
Ccn3	T	A	15: 54,615,897 (GRCm39)	I354N	possibly damaging	Het
Cd177	A	T	7: 24,444,562 (GRCm39)	F673Y	probably damaging	Het
Clmn	G	T	12: 104,743,329 (GRCm39)	S879R	probably damaging	Het
Csrp3	T	C	7: 48,489,225 (GRCm39)	H19R	possibly damaging	Het
Dmkn	T	C	7: 30,463,971 (GRCm39)	S137P	probably damaging	Het
Eno1	C	A	4: 150,331,067 (GRCm39)	Y236*	probably null	Het
Enpp7	T	A	11: 118,882,953 (GRCm39)	N342K	probably benign	Het
Fancm	T	A	12: 65,177,185 (GRCm39)		probably benign	Het
Fshr	A	T	17: 89,293,351 (GRCm39)	D442E	probably benign	Het
Gm3898	T	A	9: 43,741,362 (GRCm39)		noncoding transcript	Het
Hdac7	A	T	15: 97,709,336 (GRCm39)	S43T	probably benign	Het
Herc1	T	C	9: 66,389,263 (GRCm39)	C3927R	probably damaging	Het
Klc4	G	T	17: 46,946,355 (GRCm39)	A490D	probably damaging	Het
Lcn9	T	A	2: 25,713,663 (GRCm39)	I63N	probably damaging	Het
Lgalsl	G	A	11: 20,779,316 (GRCm39)	Q110*	probably null	Het
Lrp1b	A	T	2: 40,765,135 (GRCm39)	M2783K	possibly damaging	Het
Lrrk2	A	T	15: 91,649,948 (GRCm39)	Y1695F	probably damaging	Het
Myo1e	G	A	9: 70,277,753 (GRCm39)	E817K	probably benign	Het
Nav3	A	T	10: 109,605,264 (GRCm39)	D936E	probably damaging	Het
Necab3	T	C	2: 154,387,076 (GRCm39)		probably null	Het
Nlrp1a	T	A	11: 70,990,400 (GRCm39)	I951F	probably damaging	Het
Or5v1	T	A	17: 37,810,493 (GRCm39)	I317K	probably benign	Het
Or8b52	A	G	9: 38,576,297 (GRCm39)	I281T	possibly damaging	Het
Ppp2r5d	T	C	17: 46,998,901 (GRCm39)	S54G	probably benign	Het
Prdx6	A	G	1: 161,071,255 (GRCm39)	S146P	probably damaging	Het
Sec24a	A	T	11: 51,625,448 (GRCm39)	H258Q	probably benign	Het
Sec31a	T	C	5: 100,550,133 (GRCm39)	T194A	possibly damaging	Het
Sqstm1	T	C	11: 50,098,266 (GRCm39)	I167V	probably benign	Het
Tas1r3	T	C	4: 155,946,522 (GRCm39)	E361G	probably benign	Het
Tlr1	T	A	5: 65,083,428 (GRCm39)	Q383L	possibly damaging	Het
Vmn2r2	T	A	3: 64,024,416 (GRCm39)	M722L	probably benign	Het
Vmn2r43	T	C	7: 8,247,811 (GRCm39)	H784R	probably damaging	Het
Zfp534	T	G	4: 147,759,173 (GRCm39)	K499Q	probably damaging	Het

Other mutations in Trappc8

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01077:Trappc8	APN	18	20,970,035 (GRCm39)	missense	probably benign	0.20
IGL01367:Trappc8	APN	18	20,999,176 (GRCm39)	missense	probably benign	0.01
IGL01537:Trappc8	APN	18	20,968,061 (GRCm39)	missense	probably benign
IGL01563:Trappc8	APN	18	20,970,103 (GRCm39)	missense	probably benign	0.00
IGL01982:Trappc8	APN	18	21,007,769 (GRCm39)	splice site	probably benign
IGL02709:Trappc8	APN	18	20,970,235 (GRCm39)	missense	possibly damaging	0.94
IGL03126:Trappc8	APN	18	20,996,652 (GRCm39)	missense	probably damaging	1.00
IGL03290:Trappc8	APN	18	20,953,992 (GRCm39)	missense	probably damaging	1.00
IGL03348:Trappc8	APN	18	20,985,838 (GRCm39)	missense	probably damaging	1.00
hoppa	UTSW	18	20,969,957 (GRCm39)	missense	probably benign	0.05
Lagomorpha	UTSW	18	20,951,247 (GRCm39)	missense	probably benign	0.11
rabbit	UTSW	18	21,007,737 (GRCm39)	missense	probably damaging	1.00
E7848:Trappc8	UTSW	18	20,983,975 (GRCm39)	missense	probably damaging	0.99
R0483:Trappc8	UTSW	18	20,978,658 (GRCm39)	missense	possibly damaging	0.60
R0492:Trappc8	UTSW	18	20,999,243 (GRCm39)	missense	probably benign	0.07
R0506:Trappc8	UTSW	18	20,977,245 (GRCm39)	missense	possibly damaging	0.49
R0610:Trappc8	UTSW	18	20,970,245 (GRCm39)	missense	probably damaging	1.00
R0892:Trappc8	UTSW	18	20,964,665 (GRCm39)	critical splice donor site	probably null
R1561:Trappc8	UTSW	18	20,974,680 (GRCm39)	nonsense	probably null
R1589:Trappc8	UTSW	18	20,996,608 (GRCm39)	missense	probably damaging	1.00
R1700:Trappc8	UTSW	18	20,966,055 (GRCm39)	missense	probably damaging	1.00
R1785:Trappc8	UTSW	18	20,967,997 (GRCm39)	splice site	probably null
R1786:Trappc8	UTSW	18	20,967,997 (GRCm39)	splice site	probably null
R1989:Trappc8	UTSW	18	20,978,708 (GRCm39)	missense	probably benign	0.04
R2181:Trappc8	UTSW	18	20,952,279 (GRCm39)	critical splice donor site	probably null
R2294:Trappc8	UTSW	18	20,999,211 (GRCm39)	nonsense	probably null
R4551:Trappc8	UTSW	18	21,007,729 (GRCm39)	missense	probably benign	0.10
R4594:Trappc8	UTSW	18	20,970,005 (GRCm39)	missense	probably benign
R4631:Trappc8	UTSW	18	21,000,865 (GRCm39)	missense	probably benign	0.22
R4734:Trappc8	UTSW	18	20,974,629 (GRCm39)	nonsense	probably null
R4834:Trappc8	UTSW	18	20,958,122 (GRCm39)	missense	probably damaging	0.99
R5114:Trappc8	UTSW	18	20,977,237 (GRCm39)	missense	probably benign	0.04
R5262:Trappc8	UTSW	18	20,951,247 (GRCm39)	missense	probably benign	0.11
R5384:Trappc8	UTSW	18	20,966,119 (GRCm39)	splice site	probably null
R5476:Trappc8	UTSW	18	20,998,165 (GRCm39)	missense	probably damaging	1.00
R5503:Trappc8	UTSW	18	20,969,957 (GRCm39)	missense	probably benign	0.05
R5809:Trappc8	UTSW	18	20,951,139 (GRCm39)	missense	probably benign	0.08
R5825:Trappc8	UTSW	18	21,006,977 (GRCm39)	missense	probably damaging	1.00
R5886:Trappc8	UTSW	18	21,007,737 (GRCm39)	missense	probably damaging	1.00
R5936:Trappc8	UTSW	18	21,007,745 (GRCm39)	missense	probably damaging	1.00
R6024:Trappc8	UTSW	18	20,966,066 (GRCm39)	missense	probably damaging	0.98
R6105:Trappc8	UTSW	18	20,979,504 (GRCm39)	critical splice donor site	probably null
R6229:Trappc8	UTSW	18	21,003,802 (GRCm39)	missense	probably benign	0.00
R6376:Trappc8	UTSW	18	20,970,132 (GRCm39)	missense	probably benign	0.07
R6403:Trappc8	UTSW	18	20,999,128 (GRCm39)	missense	probably benign
R6459:Trappc8	UTSW	18	20,969,925 (GRCm39)	missense	probably benign	0.40
R6673:Trappc8	UTSW	18	21,018,314 (GRCm39)	missense	probably benign	0.01
R7041:Trappc8	UTSW	18	21,007,729 (GRCm39)	missense	probably benign	0.10
R7276:Trappc8	UTSW	18	20,951,148 (GRCm39)	missense	probably damaging	0.99
R7341:Trappc8	UTSW	18	20,985,704 (GRCm39)	missense	probably damaging	1.00
R7684:Trappc8	UTSW	18	20,996,559 (GRCm39)	missense	probably benign	0.01
R7702:Trappc8	UTSW	18	20,958,119 (GRCm39)	missense	probably damaging	0.99
R8210:Trappc8	UTSW	18	21,006,938 (GRCm39)	critical splice donor site	probably null
R8958:Trappc8	UTSW	18	21,003,667 (GRCm39)	missense	probably benign	0.02
R9037:Trappc8	UTSW	18	20,961,539 (GRCm39)	missense	probably benign	0.00
R9217:Trappc8	UTSW	18	21,000,822 (GRCm39)	missense	probably benign	0.01
R9246:Trappc8	UTSW	18	20,993,590 (GRCm39)	missense	possibly damaging	0.64
R9623:Trappc8	UTSW	18	20,983,975 (GRCm39)	missense	possibly damaging	0.91
R9766:Trappc8	UTSW	18	20,979,630 (GRCm39)	missense	possibly damaging	0.68
X0065:Trappc8	UTSW	18	20,993,579 (GRCm39)	missense	probably benign	0.03
Z1177:Trappc8	UTSW	18	20,964,720 (GRCm39)	frame shift	probably null

Predicted Primers

PCR Primer
(F):5'- ACACCTAGAAATGTTGGGCTTC -3'
(R):5'- TGTCAACAGCATCATCTATGGAC -3'

Sequencing Primer
(F):5'- CCTAGAAATGTTGGGCTTCATATTC -3'
(R):5'- CATCTATGGACTTTGGAACTGGCAC -3'

Posted On

2016-10-26