Incidental Mutation 'R5578:Fstl4'
ID 437311
Institutional Source Beutler Lab
Gene Symbol Fstl4
Ensembl Gene ENSMUSG00000036264
Gene Name follistatin-like 4
Synonyms SPIG1, B230374F23Rik
MMRRC Submission 043133-MU
Accession Numbers
Essential gene? Probably non essential (E-score: 0.151) question?
Stock # R5578 (G1)
Quality Score 225
Status Not validated
Chromosome 11
Chromosomal Location 52655461-53079365 bp(+) (GRCm39)
Type of Mutation missense
DNA Base Change (assembly) T to A at 53056608 bp (GRCm39)
Zygosity Heterozygous
Amino Acid Change Valine to Aspartic acid at position 455 (V455D)
Ref Sequence ENSEMBL: ENSMUSP00000042007 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000036796]
AlphaFold Q5STE3
Predicted Effect probably damaging
Transcript: ENSMUST00000036796
AA Change: V455D

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)
SMART Domains Protein: ENSMUSP00000042007
Gene: ENSMUSG00000036264
AA Change: V455D

DomainStartEndE-ValueType
signal peptide 1 22 N/A INTRINSIC
KAZAL 87 132 4.7e-13 SMART
Blast:IG_like 215 241 6e-7 BLAST
IGc2 260 327 1.9e-6 SMART
IGc2 352 419 1e-14 SMART
Coding Region Coverage
  • 1x: 99.9%
  • 3x: 99.6%
  • 10x: 98.2%
  • 20x: 94.9%
Validation Efficiency
MGI Phenotype PHENOTYPE: Homozygous null mice were born at expected Mendelian ratio and healthy, fertile, apparently normal with normal retinal laminar structure. [provided by MGI curators]
Allele List at MGI
Other mutations in this stock
Total: 44 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
Acap2 A G 16: 30,926,932 (GRCm39) S521P probably benign Het
Aqp11 A G 7: 97,386,665 (GRCm39) F177S probably damaging Het
Arhgap40 G T 2: 158,373,126 (GRCm39) G128V probably damaging Het
Aspm A T 1: 139,398,455 (GRCm39) K1011I probably damaging Het
Cachd1 A G 4: 100,722,203 (GRCm39) T89A probably benign Het
Cep89 ACTCCTCCTCCTCCTCCTCCTCCTC ACTCCTCCTCCTCCTCCTCCTC 7: 35,109,067 (GRCm39) probably benign Het
Cfhr2 A T 1: 139,758,806 (GRCm39) C81* probably null Het
Chd7 A G 4: 8,847,149 (GRCm39) T1631A probably benign Het
Clca4b T A 3: 144,638,196 (GRCm39) D22V probably benign Het
Csnk2a1-ps3 A G 1: 156,352,800 (GRCm39) M334V probably benign Het
Cybb C G X: 9,316,989 (GRCm39) D246H probably benign Het
Cyp39a1 T A 17: 43,991,031 (GRCm39) N113K possibly damaging Het
Dnah11 A T 12: 117,982,537 (GRCm39) V2544D probably damaging Het
Dnai3 A T 3: 145,802,983 (GRCm39) Y69* probably null Het
Esr1 A C 10: 4,919,164 (GRCm39) Q418P probably damaging Het
Fam89a T A 8: 125,467,968 (GRCm39) K115* probably null Het
Gm20730 T A 6: 43,058,474 (GRCm39) M113L probably benign Het
H2ac21 T C 3: 96,127,554 (GRCm39) V108A probably damaging Het
Hk3 C T 13: 55,159,994 (GRCm39) V327M probably damaging Het
Itm2c T A 1: 85,830,774 (GRCm39) V57E possibly damaging Het
Lrba T C 3: 86,664,814 (GRCm39) Y565H probably benign Het
Mab21l1 C T 3: 55,691,435 (GRCm39) Q341* probably null Het
Mdm2 C T 10: 117,538,192 (GRCm39) E69K possibly damaging Het
Mdn1 A T 4: 32,728,167 (GRCm39) I2709F probably benign Het
Mpp7 T C 18: 7,355,101 (GRCm39) N442D probably benign Het
Ncoa3 A G 2: 165,896,248 (GRCm39) I384V probably benign Het
Pm20d1 A G 1: 131,743,760 (GRCm39) N475S probably benign Het
Rhpn2 A G 7: 35,070,135 (GRCm39) D131G probably damaging Het
S1pr5 T A 9: 21,155,847 (GRCm39) Y193F probably damaging Het
Sdk1 A T 5: 141,598,880 (GRCm39) K182* probably null Het
Slx4 T A 16: 3,804,726 (GRCm39) E696V probably damaging Het
Smyd4 C T 11: 75,295,602 (GRCm39) P753S probably benign Het
Stambp T G 6: 83,538,782 (GRCm39) D206A probably benign Het
Sult5a1 G T 8: 123,869,860 (GRCm39) Y262* probably null Het
Taar1 A T 10: 23,796,718 (GRCm39) I139F possibly damaging Het
Tchh A T 3: 93,351,618 (GRCm39) R353* probably null Het
Thnsl2 C T 6: 71,115,749 (GRCm39) V153I probably benign Het
Trmt5 C T 12: 73,331,837 (GRCm39) probably null Het
Trpa1 T A 1: 14,957,232 (GRCm39) Y728F probably damaging Het
Usp19 T C 9: 108,370,639 (GRCm39) V126A probably benign Het
Vcan A G 13: 89,839,622 (GRCm39) V1974A probably benign Het
Vmn2r120 T A 17: 57,829,514 (GRCm39) H461L probably benign Het
Zfp445 T C 9: 122,682,402 (GRCm39) Y513C probably benign Het
Zfp84 A C 7: 29,474,856 (GRCm39) M43L possibly damaging Het
Other mutations in Fstl4
AlleleSourceChrCoordTypePredicted EffectPPH Score
IGL00093:Fstl4 APN 11 53,077,102 (GRCm39) missense probably benign 0.01
IGL00885:Fstl4 APN 11 53,039,809 (GRCm39) missense possibly damaging 0.90
IGL00915:Fstl4 APN 11 53,067,825 (GRCm39) missense probably benign
IGL00933:Fstl4 APN 11 53,077,588 (GRCm39) missense possibly damaging 0.80
IGL01022:Fstl4 APN 11 53,077,568 (GRCm39) missense probably benign 0.18
IGL01121:Fstl4 APN 11 52,705,464 (GRCm39) missense probably benign 0.00
IGL01656:Fstl4 APN 11 52,891,201 (GRCm39) missense probably damaging 1.00
IGL01805:Fstl4 APN 11 53,077,184 (GRCm39) missense probably damaging 1.00
IGL01997:Fstl4 APN 11 53,053,881 (GRCm39) nonsense probably null
IGL02386:Fstl4 APN 11 52,664,698 (GRCm39) missense probably benign 0.21
IGL02536:Fstl4 APN 11 53,024,851 (GRCm39) splice site probably benign
IGL02807:Fstl4 APN 11 53,077,501 (GRCm39) missense probably benign 0.03
IGL03037:Fstl4 APN 11 53,059,050 (GRCm39) missense possibly damaging 0.83
R0462:Fstl4 UTSW 11 53,077,229 (GRCm39) missense probably benign 0.09
R1190:Fstl4 UTSW 11 52,959,373 (GRCm39) missense probably benign
R1300:Fstl4 UTSW 11 52,959,454 (GRCm39) missense probably benign
R1626:Fstl4 UTSW 11 52,891,117 (GRCm39) nonsense probably null
R1695:Fstl4 UTSW 11 53,056,705 (GRCm39) splice site probably null
R1699:Fstl4 UTSW 11 53,059,005 (GRCm39) missense possibly damaging 0.81
R1727:Fstl4 UTSW 11 52,959,478 (GRCm39) missense probably damaging 1.00
R1752:Fstl4 UTSW 11 53,077,622 (GRCm39) missense probably benign 0.09
R1866:Fstl4 UTSW 11 53,077,225 (GRCm39) missense probably benign 0.00
R4689:Fstl4 UTSW 11 52,959,477 (GRCm39) nonsense probably null
R5126:Fstl4 UTSW 11 53,077,388 (GRCm39) missense possibly damaging 0.71
R5129:Fstl4 UTSW 11 53,077,266 (GRCm39) missense probably damaging 1.00
R5499:Fstl4 UTSW 11 52,959,374 (GRCm39) missense probably benign 0.01
R5715:Fstl4 UTSW 11 52,891,243 (GRCm39) missense possibly damaging 0.53
R6125:Fstl4 UTSW 11 53,077,130 (GRCm39) missense probably benign
R6177:Fstl4 UTSW 11 53,059,031 (GRCm39) missense probably benign 0.00
R6236:Fstl4 UTSW 11 53,077,162 (GRCm39) missense probably benign 0.00
R6311:Fstl4 UTSW 11 53,067,804 (GRCm39) missense probably damaging 1.00
R6611:Fstl4 UTSW 11 53,077,552 (GRCm39) missense probably benign 0.01
R6886:Fstl4 UTSW 11 53,077,277 (GRCm39) missense probably damaging 1.00
R7404:Fstl4 UTSW 11 53,024,898 (GRCm39) missense probably benign 0.03
R7423:Fstl4 UTSW 11 52,959,382 (GRCm39) missense possibly damaging 0.54
R7586:Fstl4 UTSW 11 52,963,256 (GRCm39) missense probably benign 0.00
R7756:Fstl4 UTSW 11 53,059,123 (GRCm39) missense possibly damaging 0.69
R7758:Fstl4 UTSW 11 53,059,123 (GRCm39) missense possibly damaging 0.69
R7775:Fstl4 UTSW 11 53,067,798 (GRCm39) nonsense probably null
R7953:Fstl4 UTSW 11 52,891,050 (GRCm39) missense probably benign 0.01
R8026:Fstl4 UTSW 11 52,959,496 (GRCm39) missense probably damaging 0.99
R8043:Fstl4 UTSW 11 52,891,050 (GRCm39) missense probably benign 0.01
R8375:Fstl4 UTSW 11 53,053,502 (GRCm39) missense possibly damaging 0.63
R8866:Fstl4 UTSW 11 52,963,233 (GRCm39) missense possibly damaging 0.54
R9103:Fstl4 UTSW 11 52,664,696 (GRCm39) missense probably benign 0.21
R9182:Fstl4 UTSW 11 53,024,905 (GRCm39) missense probably damaging 0.98
R9297:Fstl4 UTSW 11 53,024,973 (GRCm39) missense possibly damaging 0.50
R9390:Fstl4 UTSW 11 52,891,102 (GRCm39) missense probably benign
R9396:Fstl4 UTSW 11 52,664,778 (GRCm39) missense probably benign
R9447:Fstl4 UTSW 11 53,077,166 (GRCm39) missense probably damaging 1.00
R9506:Fstl4 UTSW 11 53,024,950 (GRCm39) missense probably benign 0.18
R9518:Fstl4 UTSW 11 53,056,647 (GRCm39) missense possibly damaging 0.71
R9523:Fstl4 UTSW 11 53,075,466 (GRCm39) missense probably benign 0.04
R9586:Fstl4 UTSW 11 53,077,729 (GRCm39) missense probably benign 0.02
R9594:Fstl4 UTSW 11 52,664,694 (GRCm39) missense probably benign
X0013:Fstl4 UTSW 11 53,053,446 (GRCm39) missense probably damaging 1.00
Predicted Primers PCR Primer
(F):5'- CCGTTTGCAAAGCTGGTGATAG -3'
(R):5'- GAGCTGAAGGAACCCAGACTTG -3'

Sequencing Primer
(F):5'- TGATAGGTTATGTTGGTTCCAAAC -3'
(R):5'- GAAGGAACCCAGACTTGCACAC -3'
Posted On 2016-10-26