Mutagenetix > Incidental Mutation

Incidental Mutation 'R5589:Or6c66'

437363

Institutional Source

Beutler Lab

Gene Symbol

Or6c66

Ensembl Gene

ENSMUSG00000095002

Gene Name

olfactory receptor family 6 subfamily C member 66

Synonyms

GA_x6K02T2PULF-11304679-11303744, MOR108-1, Olfr798

MMRRC Submission

043142-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.191) question?

Stock #

R5589 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

129460993-129461928 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to G at 129461319 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Threonine to Proline at position 204 (T204P)

Ref Sequence

ENSEMBL: ENSMUSP00000144716 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000079810] [ENSMUST00000204979]

AlphaFold

Q7TRH8

Predicted Effect

probably damaging
Transcript: ENSMUST00000079810
AA Change: T204P

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000078739
Gene: ENSMUSG00000095002
AA Change: T204P

Domain	Start	End	E-Value	Type
Pfam:7tm_4	28	309	5e-52	PFAM
Pfam:7tm_1	39	288	2.1e-25	PFAM

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000203614

Predicted Effect

probably damaging
Transcript: ENSMUST00000204979
AA Change: T204P

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000144716
Gene: ENSMUSG00000095002
AA Change: T204P

Domain	Start	End	E-Value	Type
Pfam:7tm_4	28	309	5e-52	PFAM
Pfam:7tm_1	39	288	2.1e-25	PFAM

Coding Region Coverage

1x: 99.9%
3x: 99.7%
10x: 98.7%
20x: 96.4%

Validation Efficiency

MGI Phenotype

FUNCTION: Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]

Allele List at MGI

Other mutations in this stock

Total: 66 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
1700093K21Rik	T	A	11: 23,468,066 (GRCm39)	M76L	probably benign	Het
Adrm1b	T	A	3: 92,336,112 (GRCm39)		probably benign	Het
Alg1	T	A	16: 5,053,086 (GRCm39)	W116R	probably benign	Het
Ano3	A	T	2: 110,715,340 (GRCm39)	S33T	probably damaging	Het
Atp2b2	T	C	6: 113,751,400 (GRCm39)	E556G	possibly damaging	Het
Brca2	T	A	5: 150,480,597 (GRCm39)	I2761K	possibly damaging	Het
Ccdc68	G	A	18: 70,079,577 (GRCm39)	G141E	probably benign	Het
Cckbr	T	C	7: 105,083,732 (GRCm39)	V220A	probably damaging	Het
Ccnd3	G	A	17: 47,909,544 (GRCm39)	R45Q	probably damaging	Het
Cdh24	G	T	14: 54,874,832 (GRCm39)	T391N	probably damaging	Het
Cldn11	A	G	3: 31,204,395 (GRCm39)	T33A	probably damaging	Het
Clec2e	T	A	6: 129,075,391 (GRCm39)	Y50F	probably benign	Het
Cntnap1	A	G	11: 101,075,944 (GRCm39)	N943D	probably benign	Het
Cspg4b	A	G	13: 113,454,484 (GRCm39)	R177G	possibly damaging	Het
Dmgdh	T	C	13: 93,813,665 (GRCm39)	V70A	probably damaging	Het
Gm14496	C	A	2: 181,637,674 (GRCm39)	Y249*	probably null	Het
Gmnc	T	A	16: 26,781,714 (GRCm39)	H105L	probably damaging	Het
Gpt2	A	G	8: 86,219,740 (GRCm39)	Y62C	probably damaging	Het
Ift80	T	C	3: 68,838,233 (GRCm39)	R413G	probably damaging	Het
Kctd16	C	A	18: 40,392,061 (GRCm39)	D216E	probably damaging	Het
Kif26b	T	C	1: 178,743,864 (GRCm39)	V873A	probably benign	Het
Klra4	G	T	6: 130,039,117 (GRCm39)	Q92K	probably benign	Het
L1td1	C	A	4: 98,626,341 (GRCm39)	N845K	possibly damaging	Het
Lama3	C	T	18: 12,605,277 (GRCm39)	T1077I	possibly damaging	Het
Loxhd1	T	C	18: 77,429,751 (GRCm39)	I230T	possibly damaging	Het
Lsg1	T	C	16: 30,399,819 (GRCm39)	N160S	probably damaging	Het
Lyzl4	G	A	9: 121,413,469 (GRCm39)	R24C	probably damaging	Het
Mib1	A	G	18: 10,794,488 (GRCm39)	N658S	probably benign	Het
Mmp8	T	C	9: 7,566,275 (GRCm39)	I377T	probably damaging	Het
Mtmr14	T	C	6: 113,238,243 (GRCm39)		probably null	Het
Myo1c	A	G	11: 75,548,414 (GRCm39)	T58A	possibly damaging	Het
Myo9a	C	T	9: 59,802,527 (GRCm39)	Q2005*	probably null	Het
Neu3	G	T	7: 99,472,636 (GRCm39)	P34T	probably benign	Het
Nlrp4b	G	A	7: 10,449,512 (GRCm39)	V205I	probably benign	Het
Or4b1b	C	T	2: 90,112,313 (GRCm39)	G202D	probably damaging	Het
Or4k38	T	C	2: 111,165,850 (GRCm39)	N191S	possibly damaging	Het
Or5h23	A	T	16: 58,906,334 (GRCm39)	S171T	probably benign	Het
Or5t15	A	G	2: 86,681,118 (GRCm39)	I308T	unknown	Het
Or9m1	T	C	2: 87,733,691 (GRCm39)	T110A	probably benign	Het
Pcsk6	T	C	7: 65,578,933 (GRCm39)		probably null	Het
Pik3c2a	A	G	7: 116,016,893 (GRCm39)	V288A	probably benign	Het
Plcd3	T	C	11: 102,968,629 (GRCm39)	D354G	probably benign	Het
Prkdc	C	A	16: 15,524,655 (GRCm39)	N1219K	probably benign	Het
Prl3d1	T	A	13: 27,278,927 (GRCm39)	Y41N	probably damaging	Het
Qrich2	C	T	11: 116,332,234 (GRCm39)	G2321R	probably damaging	Het
Rrbp1	T	C	2: 143,831,886 (GRCm39)	I94V	probably benign	Het
Serinc1	C	A	10: 57,399,262 (GRCm39)	V214L	probably benign	Het
Serpina9	G	T	12: 103,967,728 (GRCm39)	N222K	probably benign	Het
Smchd1	A	G	17: 71,747,956 (GRCm39)	Y429H	probably damaging	Het
Smyd1	C	T	6: 71,239,164 (GRCm39)	V9M	probably damaging	Het
Sostdc1	C	T	12: 36,367,246 (GRCm39)	Q141*	probably null	Het
Spam1	C	T	6: 24,796,109 (GRCm39)	T20I	probably benign	Het
Tex47	T	C	5: 7,354,834 (GRCm39)	V5A	probably benign	Het
Thbs4	T	C	13: 92,912,582 (GRCm39)		probably null	Het
Trim45	C	T	3: 100,837,257 (GRCm39)	P531L	probably damaging	Het
Tshb	A	T	3: 102,685,478 (GRCm39)	Y50*	probably null	Het
Ttn	T	C	2: 76,599,320 (GRCm39)	I19230V	probably damaging	Het
Ttn	A	T	2: 76,641,587 (GRCm39)	L5176Q	possibly damaging	Het
Uba6	G	A	5: 86,270,288 (GRCm39)	T832I	probably damaging	Het
Unc13d	A	T	11: 115,960,579 (GRCm39)	V497D	probably damaging	Het
Usp13	T	C	3: 32,892,007 (GRCm39)	V62A	probably damaging	Het
Vmn1r215	A	C	13: 23,260,189 (GRCm39)	L76F	probably damaging	Het
Vmn1r215	G	T	13: 23,260,190 (GRCm39)	G77C	probably damaging	Het
Vmn2r5	T	C	3: 64,411,497 (GRCm39)	D357G	probably damaging	Het
Zbtb40	T	C	4: 136,722,594 (GRCm39)	D828G	probably damaging	Het
Zfp74	A	T	7: 29,633,990 (GRCm39)	C573S	probably damaging	Het

Other mutations in Or6c66

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01093:Or6c66	APN	10	129,461,432 (GRCm39)	missense	probably damaging	1.00
IGL02866:Or6c66	APN	10	129,461,575 (GRCm39)	nonsense	probably null
BB009:Or6c66	UTSW	10	129,461,094 (GRCm39)	missense	probably damaging	0.97
BB019:Or6c66	UTSW	10	129,461,094 (GRCm39)	missense	probably damaging	0.97
R0743:Or6c66	UTSW	10	129,461,712 (GRCm39)	missense	probably benign	0.03
R1163:Or6c66	UTSW	10	129,461,516 (GRCm39)	missense	possibly damaging	0.79
R1192:Or6c66	UTSW	10	129,461,906 (GRCm39)	missense	probably benign
R1863:Or6c66	UTSW	10	129,461,217 (GRCm39)	missense	probably damaging	0.96
R1867:Or6c66	UTSW	10	129,461,621 (GRCm39)	missense	probably damaging	0.99
R2508:Or6c66	UTSW	10	129,461,784 (GRCm39)	missense	probably benign
R4898:Or6c66	UTSW	10	129,461,468 (GRCm39)	missense	probably benign	0.12
R4910:Or6c66	UTSW	10	129,461,676 (GRCm39)	missense	probably damaging	0.98
R4988:Or6c66	UTSW	10	129,461,930 (GRCm39)	splice site	probably null
R5361:Or6c66	UTSW	10	129,461,601 (GRCm39)	missense	probably damaging	1.00
R5382:Or6c66	UTSW	10	129,461,876 (GRCm39)	missense	probably damaging	0.98
R5693:Or6c66	UTSW	10	129,461,396 (GRCm39)	missense	probably damaging	0.99
R5790:Or6c66	UTSW	10	129,461,757 (GRCm39)	missense	probably damaging	1.00
R6966:Or6c66	UTSW	10	129,461,633 (GRCm39)	missense	probably benign	0.17
R7041:Or6c66	UTSW	10	129,461,603 (GRCm39)	missense	probably damaging	1.00
R7082:Or6c66	UTSW	10	129,461,634 (GRCm39)	missense	probably benign	0.07
R7932:Or6c66	UTSW	10	129,461,094 (GRCm39)	missense	probably damaging	0.97
R7977:Or6c66	UTSW	10	129,461,838 (GRCm39)	missense	probably benign
R7987:Or6c66	UTSW	10	129,461,838 (GRCm39)	missense	probably benign
R8299:Or6c66	UTSW	10	129,461,829 (GRCm39)	missense	probably benign	0.00
R8487:Or6c66	UTSW	10	129,461,114 (GRCm39)	missense	possibly damaging	0.50
R9110:Or6c66	UTSW	10	129,461,820 (GRCm39)	missense	possibly damaging	0.88

Predicted Primers

PCR Primer
(F):5'- TGCTATACCCTTAGTCAAAGCC -3'
(R):5'- TCTGCATGCAGCTCATTGTG -3'

Sequencing Primer
(F):5'- AGTCAAAGCCACTCCTTCCTTTG -3'
(R):5'- TGGGCCTTCAACTGGATT -3'

Posted On

2016-10-26