Incidental Mutation 'R0071:Gpr158'
| ID |
43829 |
| Institutional Source |
Beutler Lab
|
| Gene Symbol |
Gpr158
|
| Ensembl Gene |
ENSMUSG00000045967 |
| Gene Name |
G protein-coupled receptor 158 |
| Synonyms |
5330427M13Rik |
| MMRRC Submission |
038362-MU
|
| Accession Numbers |
|
| Essential gene? |
Non essential
(E-score: 0.000)
|
| Stock # |
R0071 (G1)
|
| Quality Score |
225 |
|
Status
|
Validated
|
| Chromosome |
2 |
| Chromosomal Location |
21372378-21835355 bp(+) (GRCm39) |
| Type of Mutation |
missense |
| DNA Base Change (assembly) |
C to A
at 21815479 bp (GRCm39)
|
| Zygosity |
Heterozygous |
| Amino Acid Change |
Threonine to Lysine
at position 624
(T624K)
|
| Ref Sequence |
ENSEMBL: ENSMUSP00000049708
(fasta)
|
| Gene Model |
predicted gene model for transcript(s):
[ENSMUST00000055946]
|
| AlphaFold |
Q8C419 |
| Predicted Effect |
probably benign
Transcript: ENSMUST00000055946
AA Change: T624K
PolyPhen 2
Score 0.084 (Sensitivity: 0.93; Specificity: 0.85)
|
| SMART Domains |
Protein: ENSMUSP00000049708
Gene: ENSMUSG00000045967
AA Change: T624K
| Domain | Start | End | E-Value | Type |
|---|
|
signal peptide
|
1 |
20 |
N/A |
INTRINSIC |
|
low complexity region
|
110 |
125 |
N/A |
INTRINSIC |
|
SCOP:d1edmb_
|
313 |
359 |
5e-4 |
SMART |
|
Blast:EGF
|
318 |
365 |
2e-27 |
BLAST |
|
Pfam:7tm_3
|
426 |
669 |
1.2e-35 |
PFAM |
|
low complexity region
|
840 |
863 |
N/A |
INTRINSIC |
|
| Predicted Effect |
noncoding transcript
Transcript: ENSMUST00000155760
|
| Meta Mutation Damage Score |
0.0898 |
| Coding Region Coverage |
- 1x: 99.1%
- 3x: 98.2%
- 10x: 96.0%
- 20x: 92.2%
|
| Validation Efficiency |
99% (78/79) |
| Allele List at MGI |
|
| Other mutations in this stock |
Total: 78 list
| Gene | Ref | Var | Chr/Loc | Mutation | Predicted Effect | Zygosity |
|---|
| 4930474N05Rik |
A |
G |
14: 35,812,746 (GRCm39) |
|
probably benign |
Het |
| Acot12 |
T |
C |
13: 91,929,293 (GRCm39) |
|
probably benign |
Het |
| Acrbp |
T |
C |
6: 125,027,915 (GRCm39) |
|
probably benign |
Het |
| Amotl1 |
G |
A |
9: 14,460,069 (GRCm39) |
A890V |
probably benign |
Het |
| Aox3 |
T |
A |
1: 58,211,050 (GRCm39) |
C931* |
probably null |
Het |
| Apob |
T |
A |
12: 8,052,111 (GRCm39) |
V1184E |
probably damaging |
Het |
| Arhgap44 |
A |
T |
11: 64,902,721 (GRCm39) |
L582Q |
possibly damaging |
Het |
| Bbx |
C |
T |
16: 50,100,755 (GRCm39) |
E47K |
probably benign |
Het |
| Bccip |
A |
G |
7: 133,315,960 (GRCm39) |
D72G |
probably damaging |
Het |
| Bckdha |
A |
T |
7: 25,329,868 (GRCm39) |
|
probably null |
Het |
| Bmerb1 |
A |
G |
16: 13,906,818 (GRCm39) |
D11G |
probably damaging |
Het |
| Cald1 |
C |
T |
6: 34,735,069 (GRCm39) |
|
probably benign |
Het |
| Cby2 |
A |
G |
14: 75,821,621 (GRCm39) |
S44P |
probably benign |
Het |
| Cdk11b |
T |
C |
4: 155,733,880 (GRCm39) |
|
probably benign |
Het |
| Cebpe |
G |
T |
14: 54,948,061 (GRCm39) |
R261S |
probably damaging |
Het |
| Cep95 |
C |
T |
11: 106,681,554 (GRCm39) |
|
probably benign |
Het |
| Chi3l1 |
T |
C |
1: 134,113,017 (GRCm39) |
Y150H |
probably benign |
Het |
| Chrnd |
T |
C |
1: 87,120,559 (GRCm39) |
|
probably benign |
Het |
| Clec4g |
T |
A |
8: 3,767,489 (GRCm39) |
|
probably benign |
Het |
| Cog2 |
T |
C |
8: 125,275,407 (GRCm39) |
|
probably benign |
Het |
| Coro7 |
A |
T |
16: 4,488,391 (GRCm39) |
L93Q |
probably damaging |
Het |
| Csmd3 |
T |
C |
15: 47,460,217 (GRCm39) |
T3525A |
probably benign |
Het |
| Ctsc |
G |
A |
7: 87,957,357 (GRCm39) |
|
probably benign |
Het |
| Dnajc16 |
T |
C |
4: 141,495,318 (GRCm39) |
T467A |
probably benign |
Het |
| Dnmt1 |
G |
A |
9: 20,819,916 (GRCm39) |
T1409I |
probably damaging |
Het |
| Fam227b |
T |
A |
2: 125,965,994 (GRCm39) |
N144Y |
probably benign |
Het |
| Fam83h |
A |
G |
15: 75,874,377 (GRCm39) |
S987P |
probably benign |
Het |
| Fhod1 |
A |
T |
8: 106,063,857 (GRCm39) |
|
probably null |
Het |
| Folr1 |
A |
G |
7: 101,513,130 (GRCm39) |
|
probably null |
Het |
| Glis3 |
C |
T |
19: 28,241,255 (GRCm39) |
|
probably benign |
Het |
| Gm10069 |
T |
C |
6: 128,449,688 (GRCm39) |
|
noncoding transcript |
Het |
| Golgb1 |
G |
A |
16: 36,735,865 (GRCm39) |
R1704Q |
probably benign |
Het |
| Helz2 |
T |
C |
2: 180,878,200 (GRCm39) |
Y866C |
probably damaging |
Het |
| Itpkb |
T |
A |
1: 180,160,330 (GRCm39) |
V152E |
probably damaging |
Het |
| Kcnma1 |
C |
T |
14: 23,576,835 (GRCm39) |
R236H |
probably damaging |
Het |
| Klhl32 |
A |
G |
4: 24,743,907 (GRCm39) |
V88A |
probably damaging |
Het |
| Lct |
C |
T |
1: 128,219,755 (GRCm39) |
W1631* |
probably null |
Het |
| Lipa |
T |
A |
19: 34,472,482 (GRCm39) |
K313M |
probably damaging |
Het |
| Ly75 |
T |
C |
2: 60,152,163 (GRCm39) |
K1130R |
probably benign |
Het |
| Mamdc2 |
C |
A |
19: 23,280,994 (GRCm39) |
E685* |
probably null |
Het |
| Mdm1 |
A |
G |
10: 117,982,701 (GRCm39) |
E112G |
probably damaging |
Het |
| Metrnl |
A |
T |
11: 121,606,826 (GRCm39) |
M212L |
probably benign |
Het |
| Mettl2 |
A |
G |
11: 105,022,468 (GRCm39) |
|
probably benign |
Het |
| Mxd3 |
A |
T |
13: 55,477,449 (GRCm39) |
L11Q |
probably damaging |
Het |
| Myo7a |
A |
T |
7: 97,706,037 (GRCm39) |
Y1836N |
probably damaging |
Het |
| Nsun7 |
A |
G |
5: 66,421,388 (GRCm39) |
Y118C |
probably benign |
Het |
| Obscn |
G |
A |
11: 58,955,027 (GRCm39) |
T3962M |
possibly damaging |
Het |
| Or13a20 |
A |
T |
7: 140,232,170 (GRCm39) |
I93F |
probably benign |
Het |
| Or2d36 |
A |
G |
7: 106,746,919 (GRCm39) |
Y132C |
probably damaging |
Het |
| Or5k3 |
C |
A |
16: 58,969,578 (GRCm39) |
R122S |
probably benign |
Het |
| Osbpl11 |
T |
C |
16: 33,034,708 (GRCm39) |
|
probably benign |
Het |
| Pcdhb22 |
A |
T |
18: 37,653,131 (GRCm39) |
D276V |
probably damaging |
Het |
| Pik3cb |
A |
T |
9: 98,926,918 (GRCm39) |
D886E |
probably benign |
Het |
| Pkhd1 |
T |
A |
1: 20,271,568 (GRCm39) |
Y2995F |
probably benign |
Het |
| Raver2 |
C |
T |
4: 100,977,642 (GRCm39) |
|
probably benign |
Het |
| Rhbdf1 |
A |
G |
11: 32,160,498 (GRCm39) |
L684P |
probably damaging |
Het |
| Rufy2 |
C |
A |
10: 62,824,946 (GRCm39) |
L75M |
possibly damaging |
Het |
| Sec22c |
A |
G |
9: 121,521,979 (GRCm39) |
F44L |
probably damaging |
Het |
| Sephs1 |
A |
G |
2: 4,904,371 (GRCm39) |
T250A |
probably benign |
Het |
| Serpina1a |
T |
C |
12: 103,822,002 (GRCm39) |
K310R |
probably benign |
Het |
| Shoc1 |
A |
G |
4: 59,059,643 (GRCm39) |
Y1006H |
possibly damaging |
Het |
| Sobp |
A |
G |
10: 43,033,993 (GRCm39) |
L111P |
probably damaging |
Het |
| Sparcl1 |
G |
T |
5: 104,233,707 (GRCm39) |
Y547* |
probably null |
Het |
| Spata31d1b |
G |
A |
13: 59,863,163 (GRCm39) |
A104T |
probably benign |
Het |
| Spsb3 |
A |
G |
17: 25,106,878 (GRCm39) |
D184G |
probably damaging |
Het |
| Sptan1 |
A |
T |
2: 29,893,354 (GRCm39) |
K1148* |
probably null |
Het |
| Tdrd12 |
A |
G |
7: 35,228,671 (GRCm39) |
V17A |
possibly damaging |
Het |
| Tlr9 |
A |
G |
9: 106,100,777 (GRCm39) |
T23A |
probably benign |
Het |
| Tra2b |
A |
T |
16: 22,073,151 (GRCm39) |
|
probably benign |
Het |
| Tspan15 |
A |
G |
10: 62,038,849 (GRCm39) |
|
probably benign |
Het |
| Ttc41 |
A |
G |
10: 86,572,710 (GRCm39) |
N694S |
probably benign |
Het |
| Ttn |
T |
G |
2: 76,597,813 (GRCm39) |
D19700A |
probably damaging |
Het |
| Ube3b |
G |
A |
5: 114,557,558 (GRCm39) |
G1014D |
probably damaging |
Het |
| Unc5d |
A |
G |
8: 29,209,854 (GRCm39) |
V422A |
possibly damaging |
Het |
| Vmn2r80 |
C |
T |
10: 79,007,566 (GRCm39) |
T514I |
possibly damaging |
Het |
| Zfp595 |
T |
C |
13: 67,464,917 (GRCm39) |
K452E |
possibly damaging |
Het |
| Zfp607a |
A |
G |
7: 27,577,694 (GRCm39) |
K255E |
probably damaging |
Het |
| Zxdc |
T |
G |
6: 90,347,398 (GRCm39) |
V253G |
probably damaging |
Het |
|
| Other mutations in Gpr158 |
| Allele | Source | Chr | Coord | Type | Predicted Effect | PPH Score |
|---|
|
IGL00340:Gpr158
|
APN |
2 |
21,373,494 (GRCm39) |
missense |
probably damaging |
1.00 |
|
IGL00469:Gpr158
|
APN |
2 |
21,751,606 (GRCm39) |
splice site |
probably benign |
|
|
IGL00706:Gpr158
|
APN |
2 |
21,751,584 (GRCm39) |
missense |
probably damaging |
1.00 |
|
IGL00780:Gpr158
|
APN |
2 |
21,831,629 (GRCm39) |
nonsense |
probably null |
|
|
IGL00885:Gpr158
|
APN |
2 |
21,653,832 (GRCm39) |
missense |
probably damaging |
1.00 |
|
IGL01339:Gpr158
|
APN |
2 |
21,373,842 (GRCm39) |
missense |
possibly damaging |
0.73 |
|
IGL01368:Gpr158
|
APN |
2 |
21,831,909 (GRCm39) |
missense |
probably damaging |
1.00 |
|
IGL02141:Gpr158
|
APN |
2 |
21,788,101 (GRCm39) |
missense |
probably damaging |
0.99 |
|
IGL02455:Gpr158
|
APN |
2 |
21,373,511 (GRCm39) |
missense |
probably benign |
0.00 |
|
IGL02554:Gpr158
|
APN |
2 |
21,831,407 (GRCm39) |
missense |
probably benign |
|
|
IGL02681:Gpr158
|
APN |
2 |
21,820,441 (GRCm39) |
missense |
probably damaging |
1.00 |
|
IGL02752:Gpr158
|
APN |
2 |
21,831,638 (GRCm39) |
missense |
possibly damaging |
0.95 |
|
IGL02756:Gpr158
|
APN |
2 |
21,831,890 (GRCm39) |
missense |
possibly damaging |
0.47 |
|
IGL03181:Gpr158
|
APN |
2 |
21,787,972 (GRCm39) |
missense |
probably benign |
0.02 |
|
IGL03258:Gpr158
|
APN |
2 |
21,830,085 (GRCm39) |
missense |
probably damaging |
1.00 |
|
IGL03386:Gpr158
|
APN |
2 |
21,831,057 (GRCm39) |
missense |
probably damaging |
1.00 |
|
PIT4810001:Gpr158
|
UTSW |
2 |
21,831,682 (GRCm39) |
missense |
probably benign |
0.01 |
|
R0081:Gpr158
|
UTSW |
2 |
21,831,528 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R0528:Gpr158
|
UTSW |
2 |
21,830,019 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R0560:Gpr158
|
UTSW |
2 |
21,830,085 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R0603:Gpr158
|
UTSW |
2 |
21,820,480 (GRCm39) |
missense |
possibly damaging |
0.67 |
|
R1560:Gpr158
|
UTSW |
2 |
21,831,125 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R1561:Gpr158
|
UTSW |
2 |
21,820,505 (GRCm39) |
splice site |
probably null |
|
|
R1609:Gpr158
|
UTSW |
2 |
21,788,104 (GRCm39) |
missense |
possibly damaging |
0.61 |
|
R1741:Gpr158
|
UTSW |
2 |
21,832,359 (GRCm39) |
missense |
probably benign |
0.00 |
|
R1827:Gpr158
|
UTSW |
2 |
21,832,129 (GRCm39) |
missense |
probably benign |
|
|
R1854:Gpr158
|
UTSW |
2 |
21,373,935 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R1871:Gpr158
|
UTSW |
2 |
21,820,426 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R2151:Gpr158
|
UTSW |
2 |
21,832,325 (GRCm39) |
missense |
possibly damaging |
0.82 |
|
R2273:Gpr158
|
UTSW |
2 |
21,831,674 (GRCm39) |
missense |
probably benign |
|
|
R2275:Gpr158
|
UTSW |
2 |
21,831,674 (GRCm39) |
missense |
probably benign |
|
|
R3004:Gpr158
|
UTSW |
2 |
21,831,810 (GRCm39) |
missense |
probably damaging |
0.99 |
|
R3151:Gpr158
|
UTSW |
2 |
21,581,771 (GRCm39) |
missense |
possibly damaging |
0.68 |
|
R3943:Gpr158
|
UTSW |
2 |
21,373,370 (GRCm39) |
missense |
possibly damaging |
0.65 |
|
R4238:Gpr158
|
UTSW |
2 |
21,373,362 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R4379:Gpr158
|
UTSW |
2 |
21,830,025 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R4381:Gpr158
|
UTSW |
2 |
21,832,403 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R4464:Gpr158
|
UTSW |
2 |
21,831,810 (GRCm39) |
missense |
probably damaging |
0.99 |
|
R4467:Gpr158
|
UTSW |
2 |
21,831,810 (GRCm39) |
missense |
probably damaging |
0.99 |
|
R4496:Gpr158
|
UTSW |
2 |
21,831,810 (GRCm39) |
missense |
probably damaging |
0.99 |
|
R4506:Gpr158
|
UTSW |
2 |
21,831,810 (GRCm39) |
missense |
probably damaging |
0.99 |
|
R4530:Gpr158
|
UTSW |
2 |
21,373,811 (GRCm39) |
missense |
probably benign |
0.03 |
|
R4646:Gpr158
|
UTSW |
2 |
21,831,864 (GRCm39) |
missense |
probably benign |
|
|
R4798:Gpr158
|
UTSW |
2 |
21,787,993 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R4882:Gpr158
|
UTSW |
2 |
21,830,059 (GRCm39) |
missense |
probably damaging |
0.98 |
|
R4943:Gpr158
|
UTSW |
2 |
21,831,968 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R5334:Gpr158
|
UTSW |
2 |
21,832,316 (GRCm39) |
missense |
probably benign |
0.01 |
|
R5560:Gpr158
|
UTSW |
2 |
21,831,101 (GRCm39) |
missense |
possibly damaging |
0.67 |
|
R5600:Gpr158
|
UTSW |
2 |
21,832,046 (GRCm39) |
missense |
probably benign |
|
|
R5637:Gpr158
|
UTSW |
2 |
21,788,083 (GRCm39) |
missense |
probably benign |
0.00 |
|
R5701:Gpr158
|
UTSW |
2 |
21,751,520 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R5744:Gpr158
|
UTSW |
2 |
21,373,331 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R5911:Gpr158
|
UTSW |
2 |
21,373,932 (GRCm39) |
missense |
possibly damaging |
0.95 |
|
R5991:Gpr158
|
UTSW |
2 |
21,373,319 (GRCm39) |
missense |
probably damaging |
0.99 |
|
R6200:Gpr158
|
UTSW |
2 |
21,404,227 (GRCm39) |
missense |
probably damaging |
0.97 |
|
R6306:Gpr158
|
UTSW |
2 |
21,820,422 (GRCm39) |
missense |
possibly damaging |
0.84 |
|
R6324:Gpr158
|
UTSW |
2 |
21,815,365 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R6384:Gpr158
|
UTSW |
2 |
21,831,099 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R6698:Gpr158
|
UTSW |
2 |
21,831,921 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R6997:Gpr158
|
UTSW |
2 |
21,653,802 (GRCm39) |
missense |
possibly damaging |
0.46 |
|
R7086:Gpr158
|
UTSW |
2 |
21,831,386 (GRCm39) |
missense |
probably benign |
0.01 |
|
R7175:Gpr158
|
UTSW |
2 |
21,373,113 (GRCm39) |
missense |
probably benign |
0.13 |
|
R7197:Gpr158
|
UTSW |
2 |
21,815,412 (GRCm39) |
missense |
probably damaging |
0.99 |
|
R7293:Gpr158
|
UTSW |
2 |
21,581,750 (GRCm39) |
missense |
possibly damaging |
0.47 |
|
R7427:Gpr158
|
UTSW |
2 |
21,832,129 (GRCm39) |
missense |
probably benign |
|
|
R7515:Gpr158
|
UTSW |
2 |
21,373,092 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R7730:Gpr158
|
UTSW |
2 |
21,831,158 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R8122:Gpr158
|
UTSW |
2 |
21,831,674 (GRCm39) |
missense |
probably benign |
|
|
R8311:Gpr158
|
UTSW |
2 |
21,373,701 (GRCm39) |
missense |
probably benign |
0.00 |
|
R8754:Gpr158
|
UTSW |
2 |
21,581,693 (GRCm39) |
missense |
probably benign |
0.00 |
|
R8782:Gpr158
|
UTSW |
2 |
21,404,149 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R8792:Gpr158
|
UTSW |
2 |
21,558,137 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R8842:Gpr158
|
UTSW |
2 |
21,581,751 (GRCm39) |
missense |
possibly damaging |
0.88 |
|
R9009:Gpr158
|
UTSW |
2 |
21,581,760 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R9102:Gpr158
|
UTSW |
2 |
21,830,078 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R9150:Gpr158
|
UTSW |
2 |
21,831,251 (GRCm39) |
missense |
probably benign |
0.17 |
|
R9254:Gpr158
|
UTSW |
2 |
21,373,042 (GRCm39) |
start gained |
probably benign |
|
|
R9317:Gpr158
|
UTSW |
2 |
21,832,037 (GRCm39) |
missense |
probably benign |
|
|
R9379:Gpr158
|
UTSW |
2 |
21,373,042 (GRCm39) |
start gained |
probably benign |
|
|
R9428:Gpr158
|
UTSW |
2 |
21,787,972 (GRCm39) |
missense |
probably benign |
|
|
R9497:Gpr158
|
UTSW |
2 |
21,831,825 (GRCm39) |
missense |
probably benign |
0.00 |
|
R9667:Gpr158
|
UTSW |
2 |
21,830,054 (GRCm39) |
missense |
probably damaging |
0.99 |
|
R9681:Gpr158
|
UTSW |
2 |
21,831,315 (GRCm39) |
missense |
probably damaging |
0.99 |
|
X0062:Gpr158
|
UTSW |
2 |
21,831,180 (GRCm39) |
missense |
probably damaging |
1.00 |
|
Z1176:Gpr158
|
UTSW |
2 |
21,815,501 (GRCm39) |
critical splice donor site |
probably null |
|
|
Z1177:Gpr158
|
UTSW |
2 |
21,832,083 (GRCm39) |
missense |
possibly damaging |
0.46 |
|
| Predicted Primers |
PCR Primer
(F):5'- ATTGTGTTGTAAGCAATGGCAGCAG -3'
(R):5'- TGAGCAGAACTCAGTAGAGCCCC -3'
Sequencing Primer
(F):5'- AGAGGAGACCTCTGAAAATATACAC -3'
(R):5'- CCCACACATGAGTTGTCTGAG -3'
|
| Posted On |
2013-05-29 |
Incidental Mutation