Mutagenetix > Incidental Mutation

Incidental Mutation 'R0496:Map4k4'

43896

Institutional Source

Beutler Lab

Gene Symbol

Map4k4

Ensembl Gene

ENSMUSG00000026074

Gene Name

mitogen-activated protein kinase kinase kinase kinase 4

Synonyms

9430080K19Rik, Nik

MMRRC Submission

038692-MU

Accession Numbers

Essential gene?

Essential (E-score: 1.000) question?

Stock #

R0496 (G1)

Quality Score

141

Status

Validated

Chromosome

Chromosomal Location

39940073-40065470 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to A at 40045982 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Serine to Threonine at position 754 (S754T)

Ref Sequence

ENSEMBL: ENSMUSP00000141400 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000163854] [ENSMUST00000168431] [ENSMUST00000191964] [ENSMUST00000192509] [ENSMUST00000195259] [ENSMUST00000195636] [ENSMUST00000193682] [ENSMUST00000195860]

AlphaFold

no structure available at present

Predicted Effect

probably damaging
Transcript: ENSMUST00000163854
AA Change: S754T

PolyPhen 2 Score 0.987 (Sensitivity: 0.73; Specificity: 0.96)

SMART Domains

Protein: ENSMUSP00000126961
Gene: ENSMUSG00000026074
AA Change: S754T

Domain	Start	End	E-Value	Type
S_TKc	25	289	6.87e-95	SMART
low complexity region	318	342	N/A	INTRINSIC
coiled coil region	357	494	N/A	INTRINSIC
low complexity region	503	512	N/A	INTRINSIC
low complexity region	687	698	N/A	INTRINSIC
low complexity region	721	747	N/A	INTRINSIC
low complexity region	754	763	N/A	INTRINSIC
low complexity region	811	837	N/A	INTRINSIC
low complexity region	891	904	N/A	INTRINSIC
low complexity region	919	929	N/A	INTRINSIC
CNH	970	1268	2.76e-127	SMART

Predicted Effect

unknown
Transcript: ENSMUST00000168431
AA Change: S707T

SMART Domains

Protein: ENSMUSP00000129796
Gene: ENSMUSG00000026074
AA Change: S707T

Domain	Start	End	E-Value	Type
S_TKc	25	289	6.87e-95	SMART
low complexity region	318	342	N/A	INTRINSIC
coiled coil region	357	494	N/A	INTRINSIC
low complexity region	503	512	N/A	INTRINSIC
low complexity region	633	644	N/A	INTRINSIC
low complexity region	667	693	N/A	INTRINSIC
low complexity region	700	709	N/A	INTRINSIC
low complexity region	757	783	N/A	INTRINSIC
low complexity region	837	850	N/A	INTRINSIC
low complexity region	865	875	N/A	INTRINSIC
CNH	916	1214	2.76e-127	SMART

Predicted Effect

unknown
Transcript: ENSMUST00000191865
AA Change: S111T

Predicted Effect

probably benign
Transcript: ENSMUST00000191964

SMART Domains

Protein: ENSMUSP00000141235
Gene: ENSMUSG00000026074

Domain	Start	End	E-Value	Type
low complexity region	4	28	N/A	INTRINSIC
SCOP:d1i7qa_	35	139	7e-3	SMART
low complexity region	195	206	N/A	INTRINSIC

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000192194

Predicted Effect

probably benign
Transcript: ENSMUST00000192355

Predicted Effect

unknown
Transcript: ENSMUST00000192509
AA Change: S700T

SMART Domains

Protein: ENSMUSP00000141665
Gene: ENSMUSG00000026074
AA Change: S700T

Domain	Start	End	E-Value	Type
S_TKc	25	289	6.87e-95	SMART
low complexity region	318	342	N/A	INTRINSIC
coiled coil region	357	494	N/A	INTRINSIC
low complexity region	503	512	N/A	INTRINSIC
low complexity region	633	644	N/A	INTRINSIC
low complexity region	667	693	N/A	INTRINSIC
low complexity region	700	709	N/A	INTRINSIC
low complexity region	757	783	N/A	INTRINSIC
low complexity region	837	850	N/A	INTRINSIC
low complexity region	865	875	N/A	INTRINSIC
CNH	916	1214	2.76e-127	SMART

Predicted Effect

unknown
Transcript: ENSMUST00000195259
AA Change: S677T

SMART Domains

Protein: ENSMUSP00000142056
Gene: ENSMUSG00000026074
AA Change: S677T

Domain	Start	End	E-Value	Type
S_TKc	25	289	6.87e-95	SMART
low complexity region	318	342	N/A	INTRINSIC
coiled coil region	357	494	N/A	INTRINSIC
low complexity region	503	512	N/A	INTRINSIC
low complexity region	610	621	N/A	INTRINSIC
low complexity region	644	670	N/A	INTRINSIC
low complexity region	677	686	N/A	INTRINSIC
low complexity region	731	757	N/A	INTRINSIC
low complexity region	811	824	N/A	INTRINSIC
low complexity region	839	849	N/A	INTRINSIC
CNH	890	1188	2.76e-127	SMART

Predicted Effect

unknown
Transcript: ENSMUST00000195636
AA Change: S677T

SMART Domains

Protein: ENSMUSP00000141613
Gene: ENSMUSG00000026074
AA Change: S677T

Domain	Start	End	E-Value	Type
S_TKc	25	289	3.4e-97	SMART
low complexity region	318	342	N/A	INTRINSIC
coiled coil region	357	494	N/A	INTRINSIC
low complexity region	503	512	N/A	INTRINSIC
low complexity region	610	621	N/A	INTRINSIC
low complexity region	644	670	N/A	INTRINSIC
low complexity region	677	686	N/A	INTRINSIC
low complexity region	731	757	N/A	INTRINSIC
low complexity region	836	865	N/A	INTRINSIC
low complexity region	875	888	N/A	INTRINSIC
low complexity region	903	913	N/A	INTRINSIC
CNH	954	1252	1.4e-129	SMART

Predicted Effect

probably damaging
Transcript: ENSMUST00000193682
AA Change: S623T

PolyPhen 2 Score 0.985 (Sensitivity: 0.74; Specificity: 0.96)

SMART Domains

Protein: ENSMUSP00000141862
Gene: ENSMUSG00000026074
AA Change: S623T

Domain	Start	End	E-Value	Type
S_TKc	25	289	6.87e-95	SMART
low complexity region	318	342	N/A	INTRINSIC
coiled coil region	357	494	N/A	INTRINSIC
low complexity region	503	512	N/A	INTRINSIC
low complexity region	556	567	N/A	INTRINSIC
low complexity region	590	616	N/A	INTRINSIC
low complexity region	623	632	N/A	INTRINSIC
low complexity region	680	706	N/A	INTRINSIC
low complexity region	785	814	N/A	INTRINSIC
low complexity region	824	837	N/A	INTRINSIC
low complexity region	852	862	N/A	INTRINSIC
CNH	903	1201	2.76e-127	SMART

Predicted Effect

probably damaging
Transcript: ENSMUST00000195860
AA Change: S754T

PolyPhen 2 Score 0.987 (Sensitivity: 0.73; Specificity: 0.96)

SMART Domains

Protein: ENSMUSP00000141400
Gene: ENSMUSG00000026074
AA Change: S754T

Domain	Start	End	E-Value	Type
S_TKc	25	289	6.87e-95	SMART
low complexity region	318	342	N/A	INTRINSIC
coiled coil region	357	494	N/A	INTRINSIC
low complexity region	503	512	N/A	INTRINSIC
low complexity region	687	698	N/A	INTRINSIC
low complexity region	721	747	N/A	INTRINSIC
low complexity region	754	763	N/A	INTRINSIC
low complexity region	811	837	N/A	INTRINSIC
low complexity region	891	904	N/A	INTRINSIC
low complexity region	919	929	N/A	INTRINSIC
CNH	970	1268	2.76e-127	SMART

Meta Mutation Damage Score

0.1633

Coding Region Coverage

1x: 99.1%
3x: 98.3%
10x: 96.3%
20x: 92.6%

Validation Efficiency

98% (99/101)

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] The protein encoded by this gene is a member of the serine/threonine protein kinase family. This kinase has been shown to specifically activate MAPK8/JNK. The activation of MAPK8 by this kinase is found to be inhibited by the dominant-negative mutants of MAP3K7/TAK1, MAP2K4/MKK4, and MAP2K7/MKK7, which suggests that this kinase may function through the MAP3K7-MAP2K4-MAP2K7 kinase cascade, and mediate the TNF-alpha signaling pathway. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008]
PHENOTYPE: Mice homozygous for disruptions in this gene die as embryos around day E9.5-10.5. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 97 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
1110002E22Rik	A	G	3: 137,774,005 (GRCm39)	K1065E	probably damaging	Het
4933402N03Rik	T	C	7: 130,747,860 (GRCm39)	N44S	probably benign	Het
Abca13	A	G	11: 9,241,701 (GRCm39)	D1188G	probably benign	Het
Abcb11	C	T	2: 69,108,228 (GRCm39)		probably benign	Het
Abcc8	A	T	7: 45,758,244 (GRCm39)	I1274N	probably damaging	Het
Adamtsl1	G	A	4: 86,259,435 (GRCm39)	C827Y	probably damaging	Het
Agap3	T	A	5: 24,706,241 (GRCm39)	V369E	probably damaging	Het
Ankrd13b	G	A	11: 77,363,867 (GRCm39)	R195C	probably damaging	Het
Ap3b1	A	G	13: 94,609,446 (GRCm39)		probably benign	Het
Arhgef40	A	T	14: 52,242,364 (GRCm39)		probably benign	Het
Atad5	A	G	11: 79,991,182 (GRCm39)	I692V	probably benign	Het
Atp5f1b	G	T	10: 127,922,043 (GRCm39)	R310L	possibly damaging	Het
AY358078	A	T	14: 52,040,989 (GRCm39)	M103L	unknown	Het
Bcl9l	T	G	9: 44,420,815 (GRCm39)	V1370G	probably benign	Het
Bglap3	T	A	3: 88,276,444 (GRCm39)	Q38L	probably damaging	Het
Bltp1	A	G	3: 37,041,784 (GRCm39)	T2721A	probably damaging	Het
Ccn2	G	T	10: 24,473,413 (GRCm39)	M317I	possibly damaging	Het
Cd38	T	C	5: 44,026,233 (GRCm39)	F6L	probably damaging	Het
Cela3a	A	C	4: 137,131,779 (GRCm39)	V138G	probably damaging	Het
Cemip2	A	T	19: 21,774,709 (GRCm39)	N117I	possibly damaging	Het
Clvs1	T	A	4: 9,424,241 (GRCm39)	I229N	probably damaging	Het
Cpne1	G	A	2: 155,921,339 (GRCm39)	H16Y	probably damaging	Het
Ctc1	T	C	11: 68,926,333 (GRCm39)	L1069P	probably damaging	Het
Dgkd	G	A	1: 87,864,622 (GRCm39)	S996N	probably null	Het
Dnah9	A	T	11: 65,965,961 (GRCm39)	M1685K	probably null	Het
Dnajb12	C	T	10: 59,715,623 (GRCm39)	R42*	probably null	Het
Dock5	T	C	14: 68,054,967 (GRCm39)	Q633R	probably damaging	Het
Dync2h1	A	G	9: 7,155,180 (GRCm39)	M868T	probably benign	Het
Enpp1	G	T	10: 24,547,950 (GRCm39)	H208Q	probably benign	Het
Epha7	T	A	4: 28,821,292 (GRCm39)	D152E	probably damaging	Het
Fancd2	T	C	6: 113,532,091 (GRCm39)		probably benign	Het
Gart	G	A	16: 91,419,925 (GRCm39)		probably benign	Het
Gm10964	A	T	3: 103,646,745 (GRCm39)		probably null	Het
Gpbar1	T	C	1: 74,318,140 (GRCm39)	F128L	probably benign	Het
Gsx2	T	A	5: 75,237,726 (GRCm39)	M226K	probably benign	Het
Gucd1	T	C	10: 75,347,100 (GRCm39)	D50G	possibly damaging	Het
Has1	A	G	17: 18,064,008 (GRCm39)	Y544H	probably benign	Het
Hc	A	T	2: 34,903,583 (GRCm39)	Y1024N	probably damaging	Het
Hoxa13	CCG	CCGCG	6: 52,237,618 (GRCm39)		probably null	Het
Ift122	T	A	6: 115,882,863 (GRCm39)	H659Q	probably benign	Het
Itga2	T	C	13: 114,990,435 (GRCm39)	Q902R	probably benign	Het
Itgb2l	T	C	16: 96,235,901 (GRCm39)	K181E	possibly damaging	Het
Jak3	A	T	8: 72,135,041 (GRCm39)	H558L	probably damaging	Het
Kcnh8	A	G	17: 53,032,886 (GRCm39)	T58A	probably benign	Het
Klhl6	GT	G	16: 19,775,716 (GRCm39)	279	probably null	Het
Krt33a	C	T	11: 99,903,155 (GRCm39)		probably benign	Het
Magi2	A	T	5: 20,866,357 (GRCm39)		probably benign	Het
Map4	G	A	9: 109,868,918 (GRCm39)		probably benign	Het
Mapk8ip3	A	G	17: 25,133,424 (GRCm39)		probably benign	Het
Mib1	A	G	18: 10,804,773 (GRCm39)	S918G	probably benign	Het
Mipol1	T	A	12: 57,503,963 (GRCm39)	V377D	probably damaging	Het
Mlh1	T	C	9: 111,070,624 (GRCm39)	T364A	probably benign	Het
Mta1	C	T	12: 113,094,941 (GRCm39)	Q400*	probably null	Het
Mthfd1l	C	G	10: 4,040,006 (GRCm39)	R806G	probably benign	Het
Myh13	C	A	11: 67,239,641 (GRCm39)	N730K	probably damaging	Het
Myom1	A	G	17: 71,391,301 (GRCm39)	K937E	probably damaging	Het
Naxd	T	C	8: 11,560,224 (GRCm39)		probably benign	Het
Negr1	G	T	3: 156,721,904 (GRCm39)	K159N	probably damaging	Het
Nwd2	G	T	5: 63,963,686 (GRCm39)	W1090L	probably damaging	Het
Or1e1f	T	C	11: 73,855,706 (GRCm39)	S91P	probably benign	Het
Or2j3	A	G	17: 38,615,549 (GRCm39)	S268P	probably damaging	Het
Or51v14	C	T	7: 103,261,204 (GRCm39)	A119T	probably benign	Het
Or52r1c	T	C	7: 102,734,797 (GRCm39)	I19T	probably damaging	Het
Or5d41	A	T	2: 88,054,499 (GRCm39)	Y292*	probably null	Het
Pcsk6	G	A	7: 65,576,997 (GRCm39)	S58N	probably benign	Het
Pdzrn3	G	A	6: 101,127,531 (GRCm39)	T1045I	possibly damaging	Het
Pitrm1	T	C	13: 6,618,750 (GRCm39)	L641P	probably damaging	Het
Pkd1l1	G	T	11: 8,879,430 (GRCm39)	H474N	probably damaging	Het
Pltp	A	G	2: 164,694,381 (GRCm39)		probably benign	Het
Qtrt1	C	T	9: 21,330,844 (GRCm39)	T324M	probably benign	Het
Racgap1	A	T	15: 99,537,713 (GRCm39)		probably benign	Het
Rhbg	A	G	3: 88,161,805 (GRCm39)	V50A	probably benign	Het
Rnf135	G	A	11: 80,074,776 (GRCm39)	V12M	probably damaging	Het
Rnf7l	G	T	10: 63,257,381 (GRCm39)	C46*	probably null	Het
Rufy2	T	C	10: 62,828,949 (GRCm39)	V117A	probably damaging	Het
Safb	A	G	17: 56,912,630 (GRCm39)	M866V	probably benign	Het
Slc35c2	G	T	2: 165,122,735 (GRCm39)	T183K	probably damaging	Het
Slc39a7	A	G	17: 34,248,512 (GRCm39)	L377P	probably damaging	Het
Slit1	G	A	19: 41,596,750 (GRCm39)		probably benign	Het
Spaca9	G	A	2: 28,583,022 (GRCm39)	H133Y	probably damaging	Het
Spout1	A	G	2: 30,064,983 (GRCm39)	F339S	probably benign	Het
St6gal2	A	G	17: 55,789,015 (GRCm39)	I16M	probably damaging	Het
Stat2	T	C	10: 128,112,378 (GRCm39)	M6T	probably benign	Het
Swt1	T	A	1: 151,287,021 (GRCm39)	H157L	probably benign	Het
Syne2	A	G	12: 76,085,714 (GRCm39)	N147D	possibly damaging	Het
Tmem222	A	T	4: 133,004,902 (GRCm39)	M45K	possibly damaging	Het
Tmem30a	T	A	9: 79,684,567 (GRCm39)	H95L	probably damaging	Het
Tns3	A	C	11: 8,497,262 (GRCm39)		probably benign	Het
Trpm3	A	G	19: 22,676,142 (GRCm39)	I103V	probably benign	Het
Ube2n	T	C	10: 95,377,206 (GRCm39)	F57S	probably benign	Het
Vil1	T	C	1: 74,460,499 (GRCm39)	S219P	possibly damaging	Het
Wdfy4	A	G	14: 32,862,695 (GRCm39)		probably benign	Het
Wdr7	T	C	18: 63,924,914 (GRCm39)	S966P	probably benign	Het
Wnt8a	A	G	18: 34,677,900 (GRCm39)	N103D	probably damaging	Het
Zfp523	G	A	17: 28,419,419 (GRCm39)	E186K	possibly damaging	Het
Zfp791	A	T	8: 85,836,609 (GRCm39)	D418E	probably benign	Het
Zscan20	A	G	4: 128,485,682 (GRCm39)	V192A	probably benign	Het

Other mutations in Map4k4

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00328:Map4k4	APN	1	40,043,976 (GRCm39)	missense	probably damaging	0.99
IGL00417:Map4k4	APN	1	40,053,692 (GRCm39)	missense	possibly damaging	0.92
IGL00516:Map4k4	APN	1	40,053,762 (GRCm39)	missense	probably damaging	1.00
IGL01545:Map4k4	APN	1	40,053,389 (GRCm39)	splice site	probably benign
IGL02092:Map4k4	APN	1	40,063,508 (GRCm39)	missense	probably damaging	1.00
IGL02092:Map4k4	APN	1	40,025,943 (GRCm39)	missense	probably benign	0.12
IGL02570:Map4k4	APN	1	40,019,739 (GRCm39)	missense	probably benign	0.06
IGL02626:Map4k4	APN	1	40,053,257 (GRCm39)	splice site	probably benign
IGL02993:Map4k4	APN	1	40,053,348 (GRCm39)	missense	probably damaging	0.98
IGL03178:Map4k4	APN	1	40,025,853 (GRCm39)	missense	possibly damaging	0.63
tank	UTSW	1	40,044,024 (GRCm39)	missense	possibly damaging	0.93
IGL02835:Map4k4	UTSW	1	40,049,760 (GRCm39)	missense	probably damaging	0.99
R0498:Map4k4	UTSW	1	40,029,338 (GRCm39)	missense	probably benign	0.22
R0588:Map4k4	UTSW	1	40,044,024 (GRCm39)	missense	possibly damaging	0.93
R0674:Map4k4	UTSW	1	40,042,975 (GRCm39)	missense	probably damaging	1.00
R1205:Map4k4	UTSW	1	40,043,004 (GRCm39)	missense	probably damaging	1.00
R1349:Map4k4	UTSW	1	40,060,319 (GRCm39)	missense	probably damaging	1.00
R1615:Map4k4	UTSW	1	40,045,990 (GRCm39)	splice site	probably benign
R1763:Map4k4	UTSW	1	40,039,917 (GRCm39)	splice site	probably benign
R1800:Map4k4	UTSW	1	40,062,620 (GRCm39)	missense	probably damaging	1.00
R1893:Map4k4	UTSW	1	40,040,717 (GRCm39)	missense	probably benign	0.08
R2411:Map4k4	UTSW	1	40,046,656 (GRCm39)	missense	probably damaging	0.96
R2851:Map4k4	UTSW	1	40,039,915 (GRCm39)	splice site	probably benign
R2852:Map4k4	UTSW	1	40,039,915 (GRCm39)	splice site	probably benign
R2987:Map4k4	UTSW	1	40,025,925 (GRCm39)	missense	probably damaging	1.00
R3087:Map4k4	UTSW	1	40,060,242 (GRCm39)	critical splice acceptor site	probably null
R3688:Map4k4	UTSW	1	40,024,331 (GRCm39)	splice site	probably null
R4075:Map4k4	UTSW	1	40,062,622 (GRCm39)	missense	probably damaging	0.96
R4304:Map4k4	UTSW	1	40,013,132 (GRCm39)	missense	possibly damaging	0.74
R4564:Map4k4	UTSW	1	40,028,135 (GRCm39)	missense	probably damaging	1.00
R4569:Map4k4	UTSW	1	40,039,698 (GRCm39)	missense	probably damaging	1.00
R4613:Map4k4	UTSW	1	40,056,351 (GRCm39)	missense	probably benign	0.05
R4715:Map4k4	UTSW	1	40,058,724 (GRCm39)	missense	probably damaging	1.00
R4788:Map4k4	UTSW	1	40,043,076 (GRCm39)	missense	probably benign	0.01
R4926:Map4k4	UTSW	1	40,056,385 (GRCm39)	missense	probably damaging	1.00
R4943:Map4k4	UTSW	1	40,058,754 (GRCm39)	missense	probably damaging	0.99
R5033:Map4k4	UTSW	1	40,046,662 (GRCm39)	missense	probably damaging	0.99
R5177:Map4k4	UTSW	1	40,025,922 (GRCm39)	missense	probably damaging	1.00
R5297:Map4k4	UTSW	1	40,001,377 (GRCm39)	missense	probably damaging	1.00
R5844:Map4k4	UTSW	1	40,039,036 (GRCm39)	splice site	probably benign
R5952:Map4k4	UTSW	1	40,039,082 (GRCm39)	unclassified	probably benign
R6111:Map4k4	UTSW	1	40,050,822 (GRCm39)	missense	probably benign	0.00
R6125:Map4k4	UTSW	1	40,043,125 (GRCm39)	missense	possibly damaging	0.77
R6838:Map4k4	UTSW	1	40,015,882 (GRCm39)	missense	probably damaging	1.00
R6927:Map4k4	UTSW	1	40,050,842 (GRCm39)	missense	probably benign	0.00
R7008:Map4k4	UTSW	1	40,028,131 (GRCm39)	missense	probably benign	0.44
R7164:Map4k4	UTSW	1	40,013,132 (GRCm39)	missense	possibly damaging	0.74
R7195:Map4k4	UTSW	1	40,058,829 (GRCm39)	missense	possibly damaging	0.93
R7352:Map4k4	UTSW	1	40,001,387 (GRCm39)	missense	unknown
R7589:Map4k4	UTSW	1	40,060,251 (GRCm39)	nonsense	probably null
R7816:Map4k4	UTSW	1	40,053,368 (GRCm39)	missense	possibly damaging	0.53
R7869:Map4k4	UTSW	1	40,013,204 (GRCm39)	missense	unknown
R8013:Map4k4	UTSW	1	40,001,372 (GRCm39)	missense	unknown
R8145:Map4k4	UTSW	1	40,039,694 (GRCm39)	missense
R8154:Map4k4	UTSW	1	40,060,302 (GRCm39)	nonsense	probably null
R8254:Map4k4	UTSW	1	40,045,835 (GRCm39)	missense	probably damaging	0.99
R8266:Map4k4	UTSW	1	40,050,813 (GRCm39)	missense	possibly damaging	0.53
R8375:Map4k4	UTSW	1	40,063,801 (GRCm39)	missense	possibly damaging	0.73
R8487:Map4k4	UTSW	1	40,028,136 (GRCm39)	missense	probably damaging	1.00
R8699:Map4k4	UTSW	1	40,015,910 (GRCm39)	missense	unknown
R8726:Map4k4	UTSW	1	40,043,142 (GRCm39)	missense	possibly damaging	0.95
R8907:Map4k4	UTSW	1	40,058,770 (GRCm39)	missense	probably damaging	0.97
R8956:Map4k4	UTSW	1	40,039,840 (GRCm39)	missense	probably benign	0.11
R8963:Map4k4	UTSW	1	40,039,740 (GRCm39)	missense	probably damaging	1.00
R9091:Map4k4	UTSW	1	40,042,923 (GRCm39)	missense	probably benign	0.02
R9234:Map4k4	UTSW	1	40,029,261 (GRCm39)	missense	unknown
R9270:Map4k4	UTSW	1	40,042,923 (GRCm39)	missense	probably benign	0.02
R9438:Map4k4	UTSW	1	40,045,952 (GRCm39)	missense	probably damaging	0.99
R9689:Map4k4	UTSW	1	40,058,722 (GRCm39)	missense	possibly damaging	0.95
R9771:Map4k4	UTSW	1	40,025,877 (GRCm39)	missense	unknown

Predicted Primers

PCR Primer
(F):5'- AGTATTGAGCCCCGGCTTCTTTGG -3'
(R):5'- TGATGCTGGTACGTTCAGGAGAGAC -3'

Sequencing Primer
(F):5'- TCTTTGGGAGAGAGTGGAAAAGC -3'
(R):5'- aggcaaagcatttaattccactc -3'

Posted On

2013-05-29