Mutagenetix > Incidental Mutation

Incidental Mutation 'R5601:Vmn2r82'

439030

Institutional Source

Beutler Lab

Gene Symbol

Vmn2r82

Ensembl Gene

ENSMUSG00000091468

Gene Name

vomeronasal 2, receptor 82

Synonyms

EG624845

MMRRC Submission

043153-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.068) question?

Stock #

R5601 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

79192425-79232600 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to G at 79232025 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Leucine to Valine at position 675 (L675V)

Ref Sequence

ENSEMBL: ENSMUSP00000130114 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000170596]

AlphaFold

G3UWA2

Predicted Effect

probably damaging
Transcript: ENSMUST00000170596
AA Change: L675V

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000130114
Gene: ENSMUSG00000091468
AA Change: L675V

Domain	Start	End	E-Value	Type
signal peptide	1	18	N/A	INTRINSIC
Pfam:ANF_receptor	79	474	6e-35	PFAM
Pfam:NCD3G	517	570	9.3e-22	PFAM
Pfam:7tm_3	603	838	6.5e-49	PFAM

Coding Region Coverage

1x: 99.9%
3x: 99.7%
10x: 98.7%
20x: 96.3%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 48 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Acot12	G	A	13: 91,931,029 (GRCm39)	V426I	probably benign	Het
Asmt	T	C	X: 169,110,127 (GRCm39)	V212A	probably damaging	Het
Atn1	A	G	6: 124,720,191 (GRCm39)		probably null	Het
Auts2	G	T	5: 131,505,662 (GRCm39)		probably benign	Het
AW551984	C	T	9: 39,502,563 (GRCm39)	V672M	possibly damaging	Het
Bltp3a	C	A	17: 28,103,468 (GRCm39)	A392D	probably damaging	Het
Ccdc13	G	A	9: 121,629,638 (GRCm39)	Q114*	probably null	Het
Ces2e	A	T	8: 105,656,126 (GRCm39)	I146F	probably benign	Het
Cfap44	A	G	16: 44,280,549 (GRCm39)	K1443E	probably damaging	Het
Cma2	T	A	14: 56,211,246 (GRCm39)	H179Q	possibly damaging	Het
Cpsf2	G	T	12: 101,951,614 (GRCm39)		probably null	Het
Cwh43	A	G	5: 73,575,283 (GRCm39)		probably null	Het
Dnajb12	GC	G	10: 59,728,574 (GRCm39)		probably null	Het
Dram1	A	G	10: 88,160,629 (GRCm39)	S231P	probably damaging	Het
Fbxw7	A	G	3: 84,883,515 (GRCm39)	D482G	probably damaging	Het
Fstl1	T	C	16: 37,647,161 (GRCm39)	I177T	probably benign	Het
Fut9	T	C	4: 25,620,299 (GRCm39)	T172A	probably benign	Het
Gm5134	G	A	10: 75,821,786 (GRCm39)	V207M	probably damaging	Het
Hccs	G	A	X: 168,096,597 (GRCm39)	R203C	probably damaging	Het
Hmgxb3	A	T	18: 61,270,694 (GRCm39)	F877I	probably damaging	Het
Ide	A	G	19: 37,292,379 (GRCm39)	V272A	unknown	Het
Impact	C	T	18: 13,109,064 (GRCm39)	T65I	probably benign	Het
Lgr6	C	T	1: 134,921,748 (GRCm39)	A199T	probably damaging	Het
Muc17	A	G	5: 137,166,863 (GRCm39)	S309P	probably damaging	Het
Myf6	A	C	10: 107,330,475 (GRCm39)	S31A	probably damaging	Het
Or4n4b	C	A	14: 50,536,318 (GRCm39)	W149C	probably damaging	Het
Or8b35	C	G	9: 37,904,010 (GRCm39)	T74R	possibly damaging	Het
P4hb	T	A	11: 120,462,441 (GRCm39)	E88D	possibly damaging	Het
Pcdhb9	T	C	18: 37,535,259 (GRCm39)	C418R	probably damaging	Het
Pramel51	T	C	12: 88,142,817 (GRCm39)	D267G	probably damaging	Het
Prdm4	TCTCCTCCT	TCTCCT	10: 85,728,987 (GRCm39)		probably null	Het
Prss58	A	T	6: 40,874,783 (GRCm39)	N19K	possibly damaging	Het
Ptprq	C	T	10: 107,444,291 (GRCm39)	A1438T	probably benign	Het
Rfx4	A	T	10: 84,634,442 (GRCm39)	T61S	probably damaging	Het
Sclt1	T	A	3: 41,685,354 (GRCm39)	N35Y	probably benign	Het
Sec24b	CTG	CTGGTG	3: 129,834,483 (GRCm39)		probably benign	Het
Serpinb12	T	C	1: 106,881,427 (GRCm39)	I188T	probably damaging	Het
Slc27a4	A	T	2: 29,695,672 (GRCm39)	Y69F	probably benign	Het
Slc3a1	T	C	17: 85,340,319 (GRCm39)	V247A	probably benign	Het
Slc4a2	C	T	5: 24,643,772 (GRCm39)	T854I	probably benign	Het
Stat5b	G	A	11: 100,674,001 (GRCm39)	T761M	probably damaging	Het
Stkld1	T	C	2: 26,842,717 (GRCm39)	L563P	probably damaging	Het
Tbc1d23	C	A	16: 57,018,672 (GRCm39)	C283F	probably benign	Het
Tcaf3	T	C	6: 42,564,462 (GRCm39)	N900S	possibly damaging	Het
Tkfc	T	C	19: 10,571,927 (GRCm39)	T370A	probably benign	Het
Tpr	T	C	1: 150,311,604 (GRCm39)	V1868A	possibly damaging	Het
Triobp	T	A	15: 78,857,833 (GRCm39)	W1145R	probably damaging	Het
Zfp423	T	C	8: 88,508,637 (GRCm39)	E444G	probably damaging	Het

Other mutations in Vmn2r82

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01800:Vmn2r82	APN	10	79,192,581 (GRCm39)	missense	probably benign	0.03
IGL01860:Vmn2r82	APN	10	79,214,691 (GRCm39)	missense	probably benign	0.18
IGL01927:Vmn2r82	APN	10	79,213,906 (GRCm39)	missense	probably damaging	1.00
IGL01929:Vmn2r82	APN	10	79,214,545 (GRCm39)	missense	probably damaging	1.00
IGL02028:Vmn2r82	APN	10	79,215,057 (GRCm39)	missense	probably benign
IGL02112:Vmn2r82	APN	10	79,231,833 (GRCm39)	missense	probably benign	0.19
IGL02632:Vmn2r82	APN	10	79,192,542 (GRCm39)	missense	probably benign	0.45
IGL02665:Vmn2r82	APN	10	79,215,205 (GRCm39)	missense	probably damaging	0.99
IGL02716:Vmn2r82	APN	10	79,213,678 (GRCm39)	missense	probably benign	0.20
IGL03030:Vmn2r82	APN	10	79,217,149 (GRCm39)	missense	possibly damaging	0.85
IGL03190:Vmn2r82	APN	10	79,192,643 (GRCm39)	splice site	probably null
IGL03349:Vmn2r82	APN	10	79,213,703 (GRCm39)	missense	probably benign	0.25
IGL03048:Vmn2r82	UTSW	10	79,232,460 (GRCm39)	missense	probably damaging	0.98
R0080:Vmn2r82	UTSW	10	79,232,339 (GRCm39)	missense	probably benign	0.00
R0193:Vmn2r82	UTSW	10	79,217,129 (GRCm39)	missense	probably damaging	1.00
R0217:Vmn2r82	UTSW	10	79,214,634 (GRCm39)	missense	possibly damaging	0.46
R0285:Vmn2r82	UTSW	10	79,232,391 (GRCm39)	missense	probably damaging	1.00
R1193:Vmn2r82	UTSW	10	79,213,739 (GRCm39)	nonsense	probably null
R1385:Vmn2r82	UTSW	10	79,232,325 (GRCm39)	nonsense	probably null
R1386:Vmn2r82	UTSW	10	79,214,545 (GRCm39)	missense	probably damaging	1.00
R1442:Vmn2r82	UTSW	10	79,215,201 (GRCm39)	missense	probably benign	0.03
R1467:Vmn2r82	UTSW	10	79,232,133 (GRCm39)	missense	probably benign	0.00
R1467:Vmn2r82	UTSW	10	79,232,133 (GRCm39)	missense	probably benign	0.00
R1518:Vmn2r82	UTSW	10	79,214,702 (GRCm39)	missense	probably damaging	1.00
R1538:Vmn2r82	UTSW	10	79,192,578 (GRCm39)	missense	possibly damaging	0.92
R1607:Vmn2r82	UTSW	10	79,215,253 (GRCm39)	missense	possibly damaging	0.67
R1812:Vmn2r82	UTSW	10	79,215,046 (GRCm39)	missense	probably benign	0.33
R1906:Vmn2r82	UTSW	10	79,232,344 (GRCm39)	missense	probably damaging	1.00
R1954:Vmn2r82	UTSW	10	79,231,890 (GRCm39)	missense	probably damaging	1.00
R1972:Vmn2r82	UTSW	10	79,214,680 (GRCm39)	missense	probably damaging	1.00
R2093:Vmn2r82	UTSW	10	79,231,813 (GRCm39)	missense	probably benign	0.30
R2156:Vmn2r82	UTSW	10	79,214,722 (GRCm39)	missense	probably damaging	1.00
R2202:Vmn2r82	UTSW	10	79,192,519 (GRCm39)	missense	probably benign
R2442:Vmn2r82	UTSW	10	79,221,210 (GRCm39)	missense	probably damaging	1.00
R2444:Vmn2r82	UTSW	10	79,213,702 (GRCm39)	missense	possibly damaging	0.65
R2857:Vmn2r82	UTSW	10	79,217,090 (GRCm39)	missense	probably damaging	0.98
R2858:Vmn2r82	UTSW	10	79,217,090 (GRCm39)	missense	probably damaging	0.98
R2884:Vmn2r82	UTSW	10	79,232,082 (GRCm39)	missense	probably benign	0.00
R2886:Vmn2r82	UTSW	10	79,232,082 (GRCm39)	missense	probably benign	0.00
R4369:Vmn2r82	UTSW	10	79,231,914 (GRCm39)	missense	probably benign	0.01
R4445:Vmn2r82	UTSW	10	79,214,874 (GRCm39)	missense	possibly damaging	0.87
R4589:Vmn2r82	UTSW	10	79,192,548 (GRCm39)	missense	probably damaging	1.00
R4703:Vmn2r82	UTSW	10	79,214,641 (GRCm39)	missense	probably damaging	1.00
R4908:Vmn2r82	UTSW	10	79,214,589 (GRCm39)	missense	probably benign	0.00
R4937:Vmn2r82	UTSW	10	79,215,010 (GRCm39)	missense	probably benign	0.01
R5199:Vmn2r82	UTSW	10	79,231,921 (GRCm39)	missense	probably damaging	1.00
R5391:Vmn2r82	UTSW	10	79,192,491 (GRCm39)	missense	probably null	0.01
R5635:Vmn2r82	UTSW	10	79,214,652 (GRCm39)	missense	probably benign	0.33
R6065:Vmn2r82	UTSW	10	79,221,210 (GRCm39)	missense	probably damaging	1.00
R6074:Vmn2r82	UTSW	10	79,232,377 (GRCm39)	missense	probably damaging	1.00
R6340:Vmn2r82	UTSW	10	79,231,727 (GRCm39)	missense	probably benign	0.00
R6474:Vmn2r82	UTSW	10	79,214,871 (GRCm39)	missense	possibly damaging	0.55
R6995:Vmn2r82	UTSW	10	79,232,377 (GRCm39)	missense	probably damaging	1.00
R7111:Vmn2r82	UTSW	10	79,214,605 (GRCm39)	missense	probably benign	0.22
R7212:Vmn2r82	UTSW	10	79,215,268 (GRCm39)	missense	probably benign	0.00
R7335:Vmn2r82	UTSW	10	79,214,722 (GRCm39)	missense	probably damaging	1.00
R7353:Vmn2r82	UTSW	10	79,232,452 (GRCm39)	missense	probably benign	0.11
R7354:Vmn2r82	UTSW	10	79,192,464 (GRCm39)	missense	probably benign	0.00
R7362:Vmn2r82	UTSW	10	79,232,451 (GRCm39)	missense	probably benign	0.00
R7378:Vmn2r82	UTSW	10	79,232,276 (GRCm39)	nonsense	probably null
R7430:Vmn2r82	UTSW	10	79,217,087 (GRCm39)	missense	probably damaging	1.00
R7509:Vmn2r82	UTSW	10	79,231,842 (GRCm39)	missense	possibly damaging	0.82
R7874:Vmn2r82	UTSW	10	79,232,345 (GRCm39)	missense	probably damaging	1.00
R7943:Vmn2r82	UTSW	10	79,232,079 (GRCm39)	missense	possibly damaging	0.74
R8158:Vmn2r82	UTSW	10	79,213,636 (GRCm39)	missense	probably benign	0.12
R8324:Vmn2r82	UTSW	10	79,214,727 (GRCm39)	nonsense	probably null
R8340:Vmn2r82	UTSW	10	79,217,036 (GRCm39)	missense	probably benign	0.00
R8787:Vmn2r82	UTSW	10	79,213,894 (GRCm39)	missense	probably damaging	1.00
R8929:Vmn2r82	UTSW	10	79,232,541 (GRCm39)	missense	probably benign	0.00
R9018:Vmn2r82	UTSW	10	79,232,539 (GRCm39)	missense	probably damaging	1.00
R9399:Vmn2r82	UTSW	10	79,214,768 (GRCm39)	nonsense	probably null
R9517:Vmn2r82	UTSW	10	79,213,641 (GRCm39)	nonsense	probably null
R9587:Vmn2r82	UTSW	10	79,214,936 (GRCm39)	missense	possibly damaging	0.70
R9602:Vmn2r82	UTSW	10	79,214,880 (GRCm39)	missense	probably benign	0.07
Z1088:Vmn2r82	UTSW	10	79,192,456 (GRCm39)	missense	probably damaging	1.00
Z1177:Vmn2r82	UTSW	10	79,232,369 (GRCm39)	missense	probably damaging	1.00
Z1177:Vmn2r82	UTSW	10	79,192,429 (GRCm39)	missense	probably benign	0.03

Predicted Primers

PCR Primer
(F):5'- ACTCACAGCTCTTGTTATTTGGG -3'
(R):5'- GTGTCCATGTTCAGACTGATAATCC -3'

Sequencing Primer
(F):5'- TTGGGTATTTGTAGAACACAGAGGC -3'
(R):5'- CATGTTCAGACTGATAATCCTTGTC -3'

Posted On

2016-10-26