Mutagenetix > Incidental Mutation

Incidental Mutation 'R5618:Cc2d2a'

439628

Institutional Source

Beutler Lab

Gene Symbol

Cc2d2a

Ensembl Gene

ENSMUSG00000039765

Gene Name

coiled-coil and C2 domain containing 2A

Synonyms

b2b1035Clo, 5730509K17Rik

MMRRC Submission

043277-MU

Accession Numbers

Essential gene?

Probably essential (E-score: 0.906) question?

Stock #

R5618 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

43819715-43898317 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to T at 43887249 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Glutamine to Histidine at position 1226 (Q1226H)

Ref Sequence

ENSEMBL: ENSMUSP00000114349 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000048150] [ENSMUST00000125866]

AlphaFold

Q8CFW7

Predicted Effect

probably benign
Transcript: ENSMUST00000048150
AA Change: Q1287H

PolyPhen 2 Score 0.002 (Sensitivity: 0.99; Specificity: 0.30)

SMART Domains

Protein: ENSMUSP00000048320
Gene: ENSMUSG00000039765
AA Change: Q1287H

Domain	Start	End	E-Value	Type
low complexity region	26	41	N/A	INTRINSIC
low complexity region	58	67	N/A	INTRINSIC
low complexity region	124	136	N/A	INTRINSIC
low complexity region	203	217	N/A	INTRINSIC
coiled coil region	472	501	N/A	INTRINSIC
coiled coil region	553	582	N/A	INTRINSIC
Pfam:CC2D2AN-C2	645	817	2e-36	PFAM
low complexity region	1005	1017	N/A	INTRINSIC
low complexity region	1024	1036	N/A	INTRINSIC
C2	1048	1208	3.43e-5	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000125866
AA Change: Q1226H

PolyPhen 2 Score 0.004 (Sensitivity: 0.98; Specificity: 0.59)

SMART Domains

Protein: ENSMUSP00000114349
Gene: ENSMUSG00000039765
AA Change: Q1226H

Domain	Start	End	E-Value	Type
low complexity region	9	18	N/A	INTRINSIC
low complexity region	75	87	N/A	INTRINSIC
low complexity region	154	168	N/A	INTRINSIC
coiled coil region	423	452	N/A	INTRINSIC
coiled coil region	504	533	N/A	INTRINSIC
Pfam:CC2D2AN-C2	596	768	7.7e-44	PFAM
low complexity region	970	982	N/A	INTRINSIC
C2	994	1154	2.3e-7	SMART

Coding Region Coverage

1x: 99.3%
3x: 98.6%
10x: 97.2%
20x: 95.2%

Validation Efficiency

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes a coiled-coil and calcium binding domain protein that appears to play a critical role in cilia formation. Mutations in this gene cause Meckel syndrome type 6, as well as Joubert syndrome type 9. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2009]
PHENOTYPE: Mice homozygous for a null allele exhibit embryonic lethality with multiorgan defects related to cilia biogenesis. Homozygotes for a gene trap allele show randomized body axis, holoprosencephaly, and microphthalmia. Homozygotes for an ENU-induced allele show heterotaxia, congenital heart anomalies, kidney and eye defects, polydactyly, and cleft palate. [provided by MGI curators]

Allele List at MGI

All alleles(5) : Targeted, other(4) Gene trapped(1)

Other mutations in this stock

Total: 51 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
1700028K03Rik	G	A	5: 107,696,065 (GRCm39)	W167*	probably null	Het
Acvr1	C	T	2: 58,352,955 (GRCm39)	R335Q	probably damaging	Het
Atg7	A	G	6: 114,650,660 (GRCm39)	D67G	probably damaging	Het
Avil	T	C	10: 126,846,446 (GRCm39)	F417S	possibly damaging	Het
AW551984	A	T	9: 39,502,000 (GRCm39)	L702Q	probably damaging	Het
Bcl7a	A	T	5: 123,499,265 (GRCm39)	N95Y	probably damaging	Het
Cblb	A	G	16: 51,973,031 (GRCm39)	D454G	possibly damaging	Het
Cct5	A	G	15: 31,598,161 (GRCm39)	S13P	possibly damaging	Het
Clca3a1	C	T	3: 144,710,738 (GRCm39)	E822K	probably benign	Het
Csgalnact2	A	T	6: 118,103,277 (GRCm39)	D228E	probably damaging	Het
Defb12	A	T	8: 19,164,814 (GRCm39)	M8K	possibly damaging	Het
Dipk1b	T	C	2: 26,524,887 (GRCm39)	S96P	probably damaging	Het
Dysf	A	G	6: 84,083,806 (GRCm39)	D736G	probably benign	Het
Exog	G	T	9: 119,291,817 (GRCm39)	D365Y	probably damaging	Het
Fam222b	T	G	11: 78,045,066 (GRCm39)	V81G	probably benign	Het
Fbl	A	G	7: 27,878,411 (GRCm39)	E301G	probably damaging	Het
Fez1	A	T	9: 36,755,228 (GRCm39)	N76Y	probably damaging	Het
Gnb4	C	T	3: 32,645,356 (GRCm39)	V112I	probably benign	Het
H2-Q4	T	A	17: 35,598,901 (GRCm39)	F57Y	probably damaging	Het
Ift88	T	C	14: 57,718,965 (GRCm39)	I650T	possibly damaging	Het
Kif1b	A	T	4: 149,354,346 (GRCm39)	D231E	possibly damaging	Het
Lig4	A	T	8: 10,022,021 (GRCm39)	D586E	probably benign	Het
Mlc1	C	A	15: 88,858,769 (GRCm39)	L126F	probably damaging	Het
Muc4	A	G	16: 32,754,253 (GRCm38)	T1376A	probably benign	Het
Ncoa6	A	T	2: 155,279,817 (GRCm39)	D66E	possibly damaging	Het
Or6ae1	A	G	7: 139,742,185 (GRCm39)	V226A	probably damaging	Het
Or8b44	C	T	9: 38,410,036 (GRCm39)	Q24*	probably null	Het
Pard6a	T	C	8: 106,429,546 (GRCm39)	V168A	probably damaging	Het
Pmpcb	A	G	5: 21,947,786 (GRCm39)	N163S	possibly damaging	Het
Pramel27	A	T	4: 143,577,263 (GRCm39)	E15V	possibly damaging	Het
Prdm2	C	T	4: 142,860,107 (GRCm39)	C1061Y	probably benign	Het
Prkdc	A	G	16: 15,627,476 (GRCm39)	Y3378C	probably damaging	Het
Sema6a	A	T	18: 47,415,015 (GRCm39)	V425E	probably damaging	Het
Sema7a	A	T	9: 57,867,566 (GRCm39)	E439D	possibly damaging	Het
Serpinf1	T	A	11: 75,301,010 (GRCm39)	T402S	possibly damaging	Het
Sf3a2	T	C	10: 80,640,410 (GRCm39)		probably benign	Het
Skic3	T	A	13: 76,321,545 (GRCm39)	S1346T	probably benign	Het
Smchd1	A	T	17: 71,762,722 (GRCm39)	D172E	probably damaging	Het
Spag5	T	G	11: 78,194,906 (GRCm39)	I71S	probably benign	Het
Spata31d1d	G	A	13: 59,874,214 (GRCm39)	A1107V	probably benign	Het
Svep1	A	C	4: 58,070,537 (GRCm39)	S2416R	probably benign	Het
Tbc1d9	A	G	8: 83,969,221 (GRCm39)	Y503C	probably damaging	Het
Tlr9	A	T	9: 106,101,938 (GRCm39)	I410F	possibly damaging	Het
Tmem87a	C	A	2: 120,199,787 (GRCm39)	L452F	probably benign	Het
Tmprss11d	A	T	5: 86,454,154 (GRCm39)	M217K	probably benign	Het
Uqcc4	G	A	17: 25,403,963 (GRCm39)	S101N	probably damaging	Het
Vmn2r80	T	C	10: 78,984,755 (GRCm39)	Y36H	probably benign	Het
Vmn2r87	A	T	10: 130,315,817 (GRCm39)	F83Y	probably damaging	Het
Vmp1	C	T	11: 86,554,388 (GRCm39)	R75H	probably benign	Het
Wapl	T	A	14: 34,413,863 (GRCm39)	Y242N	possibly damaging	Het
Zfp473	T	A	7: 44,391,156 (GRCm39)	D6V	probably benign	Het

Other mutations in Cc2d2a

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00392:Cc2d2a	APN	5	43,881,722 (GRCm39)	splice site	probably benign
IGL00937:Cc2d2a	APN	5	43,845,464 (GRCm39)	critical splice acceptor site	probably null
IGL01322:Cc2d2a	APN	5	43,846,345 (GRCm39)	missense	probably benign	0.00
IGL01349:Cc2d2a	APN	5	43,881,126 (GRCm39)	missense	probably benign	0.01
IGL01448:Cc2d2a	APN	5	43,841,527 (GRCm39)	missense	possibly damaging	0.65
IGL01871:Cc2d2a	APN	5	43,846,311 (GRCm39)	missense	probably damaging	0.98
IGL01947:Cc2d2a	APN	5	43,845,579 (GRCm39)	missense	probably damaging	0.96
IGL01976:Cc2d2a	APN	5	43,840,457 (GRCm39)	missense	probably benign	0.02
IGL02113:Cc2d2a	APN	5	43,842,590 (GRCm39)	splice site	probably null
IGL02364:Cc2d2a	APN	5	43,892,792 (GRCm39)	missense	probably damaging	1.00
IGL02448:Cc2d2a	APN	5	43,840,547 (GRCm39)	splice site	probably benign
IGL02458:Cc2d2a	APN	5	43,875,896 (GRCm39)	missense	probably benign	0.01
IGL02542:Cc2d2a	APN	5	43,846,252 (GRCm39)	splice site	probably benign
IGL02834:Cc2d2a	APN	5	43,871,863 (GRCm39)	nonsense	probably null
IGL02940:Cc2d2a	APN	5	43,885,636 (GRCm39)	splice site	probably null
IGL03003:Cc2d2a	APN	5	43,828,608 (GRCm39)	missense	probably benign	0.22
IGL03183:Cc2d2a	APN	5	43,889,721 (GRCm39)	missense	probably damaging	1.00
C9142:Cc2d2a	UTSW	5	43,892,799 (GRCm39)	splice site	probably benign
P0028:Cc2d2a	UTSW	5	43,841,541 (GRCm39)	missense	probably benign
R0193:Cc2d2a	UTSW	5	43,893,460 (GRCm39)	missense	probably damaging	1.00
R0201:Cc2d2a	UTSW	5	43,894,854 (GRCm39)	missense	probably damaging	1.00
R0211:Cc2d2a	UTSW	5	43,845,608 (GRCm39)	splice site	probably null
R0243:Cc2d2a	UTSW	5	43,853,980 (GRCm39)	splice site	probably benign
R0317:Cc2d2a	UTSW	5	43,864,243 (GRCm39)	critical splice donor site	probably null
R0453:Cc2d2a	UTSW	5	43,860,636 (GRCm39)	missense	probably benign	0.00
R0558:Cc2d2a	UTSW	5	43,881,729 (GRCm39)	splice site	probably benign
R0624:Cc2d2a	UTSW	5	43,887,371 (GRCm39)	missense	probably benign
R0634:Cc2d2a	UTSW	5	43,838,723 (GRCm39)	splice site	probably benign
R1503:Cc2d2a	UTSW	5	43,852,581 (GRCm39)	missense	probably damaging	1.00
R1635:Cc2d2a	UTSW	5	43,879,812 (GRCm39)	missense	probably damaging	1.00
R1686:Cc2d2a	UTSW	5	43,896,713 (GRCm39)	missense	possibly damaging	0.81
R1707:Cc2d2a	UTSW	5	43,881,030 (GRCm39)	splice site	probably null
R1715:Cc2d2a	UTSW	5	43,876,003 (GRCm39)	missense	probably damaging	0.97
R1765:Cc2d2a	UTSW	5	43,871,873 (GRCm39)	missense	probably damaging	0.99
R1794:Cc2d2a	UTSW	5	43,845,594 (GRCm39)	missense	probably damaging	1.00
R1881:Cc2d2a	UTSW	5	43,898,170 (GRCm39)	missense	probably damaging	0.99
R1917:Cc2d2a	UTSW	5	43,863,564 (GRCm39)	missense	probably damaging	1.00
R2005:Cc2d2a	UTSW	5	43,883,715 (GRCm39)	critical splice donor site	probably null
R2201:Cc2d2a	UTSW	5	43,841,375 (GRCm39)	splice site	probably benign
R2244:Cc2d2a	UTSW	5	43,889,775 (GRCm39)	missense	probably damaging	1.00
R2368:Cc2d2a	UTSW	5	43,861,230 (GRCm39)	missense	probably benign
R2442:Cc2d2a	UTSW	5	43,828,647 (GRCm39)	critical splice donor site	probably null
R2511:Cc2d2a	UTSW	5	43,892,737 (GRCm39)	missense	probably damaging	0.99
R3023:Cc2d2a	UTSW	5	43,842,593 (GRCm39)	splice site	probably null
R3147:Cc2d2a	UTSW	5	43,866,497 (GRCm39)	missense	probably damaging	1.00
R3148:Cc2d2a	UTSW	5	43,866,497 (GRCm39)	missense	probably damaging	1.00
R3426:Cc2d2a	UTSW	5	43,893,451 (GRCm39)	missense	probably benign	0.00
R3609:Cc2d2a	UTSW	5	43,869,668 (GRCm39)	missense	probably damaging	0.99
R3610:Cc2d2a	UTSW	5	43,869,668 (GRCm39)	missense	probably damaging	0.99
R3611:Cc2d2a	UTSW	5	43,869,668 (GRCm39)	missense	probably damaging	0.99
R3839:Cc2d2a	UTSW	5	43,876,056 (GRCm39)	missense	probably benign
R3870:Cc2d2a	UTSW	5	43,876,033 (GRCm39)	nonsense	probably null
R4334:Cc2d2a	UTSW	5	43,840,476 (GRCm39)	missense	probably benign	0.00
R4913:Cc2d2a	UTSW	5	43,896,665 (GRCm39)	missense	probably benign	0.12
R5179:Cc2d2a	UTSW	5	43,845,563 (GRCm39)	missense	possibly damaging	0.82
R5315:Cc2d2a	UTSW	5	43,877,775 (GRCm39)	missense	probably damaging	0.99
R5352:Cc2d2a	UTSW	5	43,863,555 (GRCm39)	missense	probably damaging	1.00
R5386:Cc2d2a	UTSW	5	43,887,383 (GRCm39)	missense	probably benign	0.01
R5538:Cc2d2a	UTSW	5	43,852,518 (GRCm39)	missense	possibly damaging	0.94
R5568:Cc2d2a	UTSW	5	43,866,433 (GRCm39)	missense	probably damaging	0.99
R5653:Cc2d2a	UTSW	5	43,879,804 (GRCm39)	missense	possibly damaging	0.81
R5817:Cc2d2a	UTSW	5	43,869,760 (GRCm39)	missense	probably damaging	1.00
R5858:Cc2d2a	UTSW	5	43,873,117 (GRCm39)	missense	probably damaging	1.00
R5905:Cc2d2a	UTSW	5	43,869,768 (GRCm39)	missense	probably benign
R5912:Cc2d2a	UTSW	5	43,877,772 (GRCm39)	missense	probably damaging	0.97
R6073:Cc2d2a	UTSW	5	43,887,317 (GRCm39)	missense	probably damaging	1.00
R6084:Cc2d2a	UTSW	5	43,826,015 (GRCm39)	missense	probably benign
R6142:Cc2d2a	UTSW	5	43,860,540 (GRCm39)	missense	probably damaging	0.97
R6176:Cc2d2a	UTSW	5	43,866,455 (GRCm39)	missense	probably benign	0.32
R6238:Cc2d2a	UTSW	5	43,828,577 (GRCm39)	missense	probably benign	0.11
R6381:Cc2d2a	UTSW	5	43,873,118 (GRCm39)	missense	possibly damaging	0.69
R6404:Cc2d2a	UTSW	5	43,861,416 (GRCm39)	missense	possibly damaging	0.58
R6455:Cc2d2a	UTSW	5	43,896,754 (GRCm39)	missense	possibly damaging	0.69
R6695:Cc2d2a	UTSW	5	43,876,019 (GRCm39)	missense	probably damaging	0.99
R6805:Cc2d2a	UTSW	5	43,838,673 (GRCm39)	missense	probably damaging	1.00
R6919:Cc2d2a	UTSW	5	43,860,557 (GRCm39)	missense	probably benign	0.19
R6970:Cc2d2a	UTSW	5	43,875,927 (GRCm39)	missense	probably damaging	1.00
R7024:Cc2d2a	UTSW	5	43,891,271 (GRCm39)	missense	probably benign	0.10
R7054:Cc2d2a	UTSW	5	43,857,321 (GRCm39)	nonsense	probably null
R7071:Cc2d2a	UTSW	5	43,866,455 (GRCm39)	missense	probably benign	0.13
R7098:Cc2d2a	UTSW	5	43,840,481 (GRCm39)	missense	probably benign	0.00
R7366:Cc2d2a	UTSW	5	43,887,332 (GRCm39)	missense	probably damaging	1.00
R7908:Cc2d2a	UTSW	5	43,864,188 (GRCm39)	missense	probably benign	0.00
R7920:Cc2d2a	UTSW	5	43,896,651 (GRCm39)	missense	probably benign	0.09
R7950:Cc2d2a	UTSW	5	43,852,638 (GRCm39)	critical splice donor site	probably null
R8007:Cc2d2a	UTSW	5	43,863,442 (GRCm39)	missense	possibly damaging	0.71
R8117:Cc2d2a	UTSW	5	43,869,781 (GRCm39)	missense	probably damaging	1.00
R8123:Cc2d2a	UTSW	5	43,867,896 (GRCm39)	missense	probably benign
R8179:Cc2d2a	UTSW	5	43,857,295 (GRCm39)	missense	probably damaging	0.96
R8279:Cc2d2a	UTSW	5	43,893,487 (GRCm39)	missense	probably benign	0.01
R8293:Cc2d2a	UTSW	5	43,845,570 (GRCm39)	missense	probably damaging	0.97
R8480:Cc2d2a	UTSW	5	43,842,486 (GRCm39)	splice site	probably null
R8482:Cc2d2a	UTSW	5	43,852,581 (GRCm39)	missense	probably damaging	1.00
R8731:Cc2d2a	UTSW	5	43,892,788 (GRCm39)	missense	probably damaging	1.00
R8780:Cc2d2a	UTSW	5	43,896,692 (GRCm39)	missense	probably damaging	1.00
R8784:Cc2d2a	UTSW	5	43,860,645 (GRCm39)	missense	possibly damaging	0.90
R8871:Cc2d2a	UTSW	5	43,857,285 (GRCm39)	missense	possibly damaging	0.71
R8972:Cc2d2a	UTSW	5	43,867,884 (GRCm39)	missense	probably benign
R9122:Cc2d2a	UTSW	5	43,831,081 (GRCm39)	missense	probably null	0.07
R9125:Cc2d2a	UTSW	5	43,860,563 (GRCm39)	missense	probably benign
R9203:Cc2d2a	UTSW	5	43,891,179 (GRCm39)	missense	probably benign	0.01
R9310:Cc2d2a	UTSW	5	43,852,488 (GRCm39)	missense	probably damaging	1.00
R9343:Cc2d2a	UTSW	5	43,875,999 (GRCm39)	missense	probably damaging	1.00
R9353:Cc2d2a	UTSW	5	43,860,691 (GRCm39)	critical splice donor site	probably null
Z1177:Cc2d2a	UTSW	5	43,860,546 (GRCm39)	missense	probably benign

Predicted Primers

PCR Primer
(F):5'- TAAGGCTTGCCAATTCACTACTCTC -3'
(R):5'- TTGGCTAAGGCAGACTCACC -3'

Sequencing Primer
(F):5'- TTAAATAAAGCTGCAGAGATACCAG -3'
(R):5'- TAAGGCAGACTCACCGCAGTG -3'

Posted On

2016-11-08