Mutagenetix > Incidental Mutation

Incidental Mutation 'R5643:Cobl'

440876

Institutional Source

Beutler Lab

Gene Symbol

Cobl

Ensembl Gene

ENSMUSG00000020173

Gene Name

cordon-bleu WH2 repeat

Synonyms

C530045F18Rik

MMRRC Submission

043291-MU

Accession Numbers

Essential gene?

Non essential (E-score: 0.000) question?

Stock #

R5643 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

12186676-12415022 bp(-) (GRCm39)

Type of Mutation

splice site

DNA Base Change (assembly)

A to T at 12256948 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Ref Sequence

ENSEMBL: ENSMUSP00000133470 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000046755] [ENSMUST00000109650] [ENSMUST00000109651] [ENSMUST00000172827] [ENSMUST00000172919] [ENSMUST00000174874] [ENSMUST00000172956]

AlphaFold

Q5NBX1

Predicted Effect

probably benign
Transcript: ENSMUST00000046755

SMART Domains

Protein: ENSMUSP00000045693
Gene: ENSMUSG00000020173

Domain	Start	End	E-Value	Type
low complexity region	14	30	N/A	INTRINSIC
Pfam:Cobl	144	235	2.2e-46	PFAM
low complexity region	328	333	N/A	INTRINSIC
low complexity region	360	376	N/A	INTRINSIC
low complexity region	408	433	N/A	INTRINSIC
low complexity region	468	482	N/A	INTRINSIC
low complexity region	526	541	N/A	INTRINSIC
coiled coil region	564	589	N/A	INTRINSIC
WH2	1185	1205	1.32e0	SMART
WH2	1225	1245	6.36e-3	SMART
low complexity region	1276	1296	N/A	INTRINSIC
WH2	1313	1333	3.91e-3	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000109650

SMART Domains

Protein: ENSMUSP00000105277
Gene: ENSMUSG00000020173

Domain	Start	End	E-Value	Type
low complexity region	14	30	N/A	INTRINSIC
Pfam:Cobl	182	260	1.6e-40	PFAM
low complexity region	303	308	N/A	INTRINSIC
low complexity region	335	351	N/A	INTRINSIC
low complexity region	386	400	N/A	INTRINSIC
low complexity region	444	459	N/A	INTRINSIC
coiled coil region	482	507	N/A	INTRINSIC
WH2	1103	1123	1.32e0	SMART
WH2	1143	1163	6.36e-3	SMART
low complexity region	1194	1214	N/A	INTRINSIC
WH2	1231	1251	3.91e-3	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000109651

SMART Domains

Protein: ENSMUSP00000105278
Gene: ENSMUSG00000020173

Domain	Start	End	E-Value	Type
low complexity region	14	30	N/A	INTRINSIC
Pfam:Cobl	182	260	1.2e-40	PFAM
low complexity region	303	308	N/A	INTRINSIC
low complexity region	335	351	N/A	INTRINSIC
low complexity region	383	408	N/A	INTRINSIC
low complexity region	443	457	N/A	INTRINSIC
low complexity region	501	516	N/A	INTRINSIC
coiled coil region	539	564	N/A	INTRINSIC
WH2	1160	1180	1.32e0	SMART
WH2	1200	1220	6.36e-3	SMART
low complexity region	1251	1271	N/A	INTRINSIC
WH2	1288	1308	3.91e-3	SMART

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000130572

Predicted Effect

probably benign
Transcript: ENSMUST00000136549

SMART Domains

Protein: ENSMUSP00000114779
Gene: ENSMUSG00000020173

Domain	Start	End	E-Value	Type
low complexity region	83	88	N/A	INTRINSIC
low complexity region	115	131	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000172827

SMART Domains

Protein: ENSMUSP00000133368
Gene: ENSMUSG00000020173

Domain	Start	End	E-Value	Type
Pfam:Cobl	1	32	1.2e-9	PFAM
low complexity region	100	105	N/A	INTRINSIC
low complexity region	132	148	N/A	INTRINSIC
low complexity region	183	197	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000172919

SMART Domains

Protein: ENSMUSP00000133669
Gene: ENSMUSG00000020173

Domain	Start	End	E-Value	Type
low complexity region	14	30	N/A	INTRINSIC
Pfam:Cobl	182	260	2.6e-41	PFAM
low complexity region	328	333	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000174874

SMART Domains

Protein: ENSMUSP00000133470
Gene: ENSMUSG00000020173

Domain	Start	End	E-Value	Type
low complexity region	6	23	N/A	INTRINSIC
Pfam:Cobl	175	253	1.2e-40	PFAM
low complexity region	321	326	N/A	INTRINSIC
low complexity region	353	369	N/A	INTRINSIC
low complexity region	401	426	N/A	INTRINSIC
low complexity region	461	475	N/A	INTRINSIC
low complexity region	519	534	N/A	INTRINSIC
coiled coil region	557	582	N/A	INTRINSIC
WH2	1178	1198	1.32e0	SMART
WH2	1218	1238	6.36e-3	SMART
low complexity region	1269	1289	N/A	INTRINSIC
WH2	1306	1326	3.91e-3	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000172956

SMART Domains

Protein: ENSMUSP00000134372
Gene: ENSMUSG00000020173

Domain	Start	End	E-Value	Type
low complexity region	14	30	N/A	INTRINSIC
Pfam:Cobl	182	260	2.4e-41	PFAM
low complexity region	303	308	N/A	INTRINSIC
low complexity region	335	351	N/A	INTRINSIC

Coding Region Coverage

1x: 99.3%
3x: 98.6%
10x: 97.2%
20x: 95.2%

Validation Efficiency

94% (110/117)

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes a protein that contains WH2 domains (WASP, Wiskott-Aldrich syndrome protein, homology domain-2) that interact with actin. The encoded actin regulator protein is required for growth and assembly of brush border microvilli that play a role in maintaining intestinal homeostasis. A similar protein in mouse functions in midbrain neural tube closure. A pseudogene of this gene is located on chromosome X. [provided by RefSeq, Oct 2016]
PHENOTYPE: Animals homozygous for this mutation do not display a phenotype. However, the allele exacerbates the neural tube defects seen in the loop tail mouse. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 102 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Ahnak	A	G	19: 8,988,021 (GRCm39)	K3102E	possibly damaging	Het
Akap13	T	C	7: 75,351,902 (GRCm39)		probably null	Het
Akp3	A	T	1: 87,055,485 (GRCm39)	T511S	unknown	Het
Alkal2	T	A	12: 30,934,889 (GRCm39)	L36Q	probably damaging	Het
Arhgef17	A	T	7: 100,529,218 (GRCm39)	V523E	probably damaging	Het
Asah1	G	T	8: 41,813,332 (GRCm39)	T27K	possibly damaging	Het
Bag2	A	G	1: 33,786,034 (GRCm39)	V96A	probably damaging	Het
Bicd1	C	T	6: 149,421,901 (GRCm39)	A874V	probably damaging	Het
C4b	T	C	17: 34,961,391 (GRCm39)	I189M	probably benign	Het
Calcr	A	G	6: 3,708,538 (GRCm39)	I216T	probably damaging	Het
Cdk5rap2	T	A	4: 70,184,970 (GRCm39)	D1160V	probably damaging	Het
Cep128	A	T	12: 91,315,625 (GRCm39)	I87K	probably damaging	Het
Cep170b	C	G	12: 112,707,275 (GRCm39)	H1256Q	probably benign	Het
Chd2	A	T	7: 73,134,232 (GRCm39)	V705E	probably damaging	Het
Clec12b	T	A	6: 129,356,923 (GRCm39)	I172L	probably benign	Het
Col5a2	T	A	1: 45,429,202 (GRCm39)	D972V	probably damaging	Het
Csrp1	A	T	1: 135,678,797 (GRCm39)	N174I	probably damaging	Het
Dnah7a	T	A	1: 53,444,866 (GRCm39)	H3946L	probably benign	Het
Dnajc21	A	T	15: 10,462,001 (GRCm39)	D133E	probably benign	Het
Dvl3	T	A	16: 20,345,026 (GRCm39)	I353N	probably damaging	Het
Elavl3	A	T	9: 21,930,029 (GRCm39)	S292T	probably benign	Het
Ephb2	T	C	4: 136,498,923 (GRCm39)	N52S	probably damaging	Het
Gaa	T	A	11: 119,171,361 (GRCm39)	M671K	possibly damaging	Het
Gabrg3	T	C	7: 56,423,032 (GRCm39)	D222G	possibly damaging	Het
Gk5	C	T	9: 96,022,709 (GRCm39)	Q182*	probably null	Het
Gm14403	A	T	2: 177,199,054 (GRCm39)	H50L	possibly damaging	Het
Gzma	T	C	13: 113,234,794 (GRCm39)	T66A	probably damaging	Het
Hint2	T	C	4: 43,656,445 (GRCm39)		probably benign	Het
Hnmt	A	T	2: 23,904,251 (GRCm39)	W137R	probably damaging	Het
Hoga1	T	C	19: 42,048,402 (GRCm39)	V90A	probably benign	Het
Idua	A	T	5: 108,828,090 (GRCm39)		probably benign	Het
Kif1a	A	C	1: 92,983,489 (GRCm39)	S669R	probably damaging	Het
Klhdc7b	A	T	15: 89,271,862 (GRCm39)	M915L	possibly damaging	Het
Klhl41	A	G	2: 69,500,815 (GRCm39)	Y92C	probably damaging	Het
Klrc2	A	T	6: 129,633,420 (GRCm39)	C186S	probably damaging	Het
Lmo7	A	G	14: 102,166,772 (GRCm39)		probably benign	Het
Lrriq1	T	C	10: 103,051,301 (GRCm39)	M484V	probably benign	Het
Lzts1	A	G	8: 69,591,729 (GRCm39)	S140P	possibly damaging	Het
Mgat5b	T	A	11: 116,864,226 (GRCm39)	V464E	probably damaging	Het
Mms19	A	T	19: 41,944,305 (GRCm39)	D298E	possibly damaging	Het
Muc5ac	T	C	7: 141,347,452 (GRCm39)		probably null	Het
Mycbp2	T	A	14: 103,524,770 (GRCm39)	K597I	probably damaging	Het
Myo18a	C	A	11: 77,745,513 (GRCm39)	D1619E	probably benign	Het
Nfx1	T	C	4: 40,984,973 (GRCm39)	W366R	probably null	Het
Nipbl	C	T	15: 8,388,391 (GRCm39)	V410I	probably benign	Het
Or14j10	T	A	17: 37,935,323 (GRCm39)	I68F	probably benign	Het
Or4c101	T	A	2: 88,389,849 (GRCm39)	M1K	probably null	Het
Or4f60	A	T	2: 111,902,013 (GRCm39)	M305K	probably benign	Het
Or52h1	T	C	7: 103,829,091 (GRCm39)	I175V	probably benign	Het
Or5b95	A	T	19: 12,658,336 (GRCm39)	Y288F	probably damaging	Het
Or7g32	A	T	9: 19,388,853 (GRCm39)	M231K	probably benign	Het
Or8u10	T	C	2: 85,915,503 (GRCm39)	N206S	probably damaging	Het
Otog	A	T	7: 45,936,871 (GRCm39)	T1527S	probably damaging	Het
P2ry12	A	T	3: 59,125,516 (GRCm39)	M53K	possibly damaging	Het
Pask	A	G	1: 93,265,065 (GRCm39)		probably null	Het
Pcca	G	A	14: 123,124,481 (GRCm39)	C684Y	probably damaging	Het
Pcdhb10	A	G	18: 37,546,219 (GRCm39)	T432A	possibly damaging	Het
Peak1	T	C	9: 56,166,039 (GRCm39)	N630D	probably damaging	Het
Plbd2	A	T	5: 120,631,231 (GRCm39)		probably null	Het
Plekhg5	T	C	4: 152,188,797 (GRCm39)	V200A	probably benign	Het
Pola2	A	G	19: 6,011,198 (GRCm39)	V42A	probably benign	Het
Ppfibp2	T	A	7: 107,337,097 (GRCm39)	W572R	probably damaging	Het
Ppp6r1	C	T	7: 4,636,771 (GRCm39)	E679K	probably benign	Het
Pramel11	A	T	4: 143,622,337 (GRCm39)	H339Q	probably damaging	Het
Prdx3	G	A	19: 60,859,963 (GRCm39)	A70V	probably damaging	Het
Prkd2	T	C	7: 16,577,717 (GRCm39)	F57L	probably benign	Het
Prodh2	A	G	7: 30,206,171 (GRCm39)	T324A	possibly damaging	Het
Ptdss1	T	C	13: 67,120,604 (GRCm39)	F267L	probably damaging	Het
Rai14	T	A	15: 10,593,137 (GRCm39)	H169L	probably benign	Het
Rere	A	G	4: 150,701,700 (GRCm39)	H1360R	probably damaging	Het
Rfc3	C	T	5: 151,573,444 (GRCm39)	V40I	probably benign	Het
Rims1	T	A	1: 22,577,590 (GRCm39)	T219S	probably damaging	Het
Rnf169	T	C	7: 99,576,338 (GRCm39)	R289G	possibly damaging	Het
Senp7	T	C	16: 56,004,512 (GRCm39)		silent	Het
Sfmbt2	A	T	2: 10,573,184 (GRCm39)	I571F	probably damaging	Het
Slc11a2	C	A	15: 100,301,068 (GRCm39)	K328N	probably benign	Het
Slc25a10	A	G	11: 120,387,202 (GRCm39)		probably benign	Het
Slc38a8	T	C	8: 120,207,488 (GRCm39)	*433W	probably null	Het
Slco1a5	A	C	6: 142,183,320 (GRCm39)		probably null	Het
Smc6	T	A	12: 11,339,995 (GRCm39)	N434K	probably benign	Het
Syndig1	A	T	2: 149,741,428 (GRCm39)	I5F	possibly damaging	Het
Syt5	T	C	7: 4,546,018 (GRCm39)	Q124R	probably benign	Het
Taok3	A	C	5: 117,344,785 (GRCm39)	M171L	probably benign	Het
Tbrg1	T	C	9: 37,560,709 (GRCm39)	D389G	probably benign	Het
Tcaf2	G	A	6: 42,619,707 (GRCm39)	R107C	possibly damaging	Het
Tex14	C	A	11: 87,426,452 (GRCm39)	H1159Q	probably damaging	Het
Tmprss11d	T	A	5: 86,474,388 (GRCm39)	M190L	probably benign	Het
Ttn	T	A	2: 76,768,867 (GRCm39)	T2856S	probably damaging	Het
Ubr4	T	A	4: 139,171,998 (GRCm39)	M2997K	probably damaging	Het
Unc5c	C	T	3: 141,383,886 (GRCm39)	A88V	probably damaging	Het
Use1	T	C	8: 71,820,398 (GRCm39)		probably benign	Het
Vmn1r43	T	C	6: 89,847,354 (GRCm39)	N44S	probably damaging	Het
Vmn1r89	T	A	7: 12,954,146 (GRCm39)	V294D	possibly damaging	Het
Vmn2r11	A	T	5: 109,194,869 (GRCm39)	V819E	probably damaging	Het
Vmn2r120	T	A	17: 57,831,977 (GRCm39)	M271L	probably benign	Het
Vmn2r52	T	C	7: 9,905,059 (GRCm39)	Y260C	probably damaging	Het
Vmn2r67	T	A	7: 84,799,151 (GRCm39)	R519*	probably null	Het
Vmn2r69	T	A	7: 85,056,404 (GRCm39)	D578V	probably damaging	Het
Vmn2r85	T	C	10: 130,262,343 (GRCm39)	Y132C	probably damaging	Het
Wdr74	T	A	19: 8,715,240 (GRCm39)	V133E	probably damaging	Het
Zfp318	T	A	17: 46,720,170 (GRCm39)		probably benign	Het
Zfp810	A	T	9: 22,194,467 (GRCm39)	S74T	probably benign	Het

Other mutations in Cobl

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00338:Cobl	APN	11	12,325,813 (GRCm39)	missense	possibly damaging	0.89
IGL00698:Cobl	APN	11	12,203,722 (GRCm39)	missense	probably benign	0.41
IGL00772:Cobl	APN	11	12,216,985 (GRCm39)	missense	probably benign	0.02
IGL00922:Cobl	APN	11	12,204,866 (GRCm39)	missense	probably damaging	1.00
IGL00985:Cobl	APN	11	12,204,843 (GRCm39)	missense	probably damaging	1.00
IGL01641:Cobl	APN	11	12,259,641 (GRCm39)	nonsense	probably null
IGL01722:Cobl	APN	11	12,203,987 (GRCm39)	missense	probably benign	0.00
IGL01734:Cobl	APN	11	12,204,980 (GRCm39)	splice site	probably benign
IGL01924:Cobl	APN	11	12,204,596 (GRCm39)	missense	probably benign	0.30
IGL02105:Cobl	APN	11	12,199,651 (GRCm39)	missense	probably damaging	1.00
IGL02326:Cobl	APN	11	12,336,712 (GRCm39)	missense	possibly damaging	0.69
IGL02342:Cobl	APN	11	12,203,672 (GRCm39)	missense	possibly damaging	0.64
IGL02426:Cobl	APN	11	12,204,351 (GRCm39)	nonsense	probably null
IGL02754:Cobl	APN	11	12,204,370 (GRCm39)	missense	probably damaging	1.00
IGL02754:Cobl	APN	11	12,204,371 (GRCm39)	missense	probably damaging	1.00
IGL02811:Cobl	APN	11	12,203,285 (GRCm39)	missense	possibly damaging	0.56
IGL02859:Cobl	APN	11	12,319,602 (GRCm39)	missense	probably damaging	1.00
IGL02999:Cobl	APN	11	12,293,869 (GRCm39)	missense	possibly damaging	0.71
IGL03030:Cobl	APN	11	12,204,241 (GRCm39)	missense	possibly damaging	0.80
IGL03191:Cobl	APN	11	12,203,364 (GRCm39)	missense	probably benign	0.00
PIT4418001:Cobl	UTSW	11	12,206,240 (GRCm39)	missense	possibly damaging	0.79
PIT4480001:Cobl	UTSW	11	12,203,592 (GRCm39)	missense	probably benign
PIT4495001:Cobl	UTSW	11	12,204,596 (GRCm39)	missense	probably benign	0.00
R0031:Cobl	UTSW	11	12,204,945 (GRCm39)	missense	probably benign	0.36
R0241:Cobl	UTSW	11	12,204,524 (GRCm39)	missense	probably benign	0.25
R0241:Cobl	UTSW	11	12,204,524 (GRCm39)	missense	probably benign	0.25
R0322:Cobl	UTSW	11	12,217,072 (GRCm39)	missense	probably damaging	1.00
R0597:Cobl	UTSW	11	12,204,699 (GRCm39)	missense	probably benign	0.24
R0733:Cobl	UTSW	11	12,315,167 (GRCm39)	missense	probably benign	0.31
R0734:Cobl	UTSW	11	12,325,971 (GRCm39)	missense	probably damaging	1.00
R0784:Cobl	UTSW	11	12,216,843 (GRCm39)	splice site	probably benign
R0884:Cobl	UTSW	11	12,325,908 (GRCm39)	missense	possibly damaging	0.89
R1065:Cobl	UTSW	11	12,204,327 (GRCm39)	missense	possibly damaging	0.67
R1331:Cobl	UTSW	11	12,325,853 (GRCm39)	missense	probably damaging	0.96
R1892:Cobl	UTSW	11	12,203,258 (GRCm39)	missense	probably damaging	0.99
R2847:Cobl	UTSW	11	12,328,342 (GRCm39)	missense	probably damaging	1.00
R2848:Cobl	UTSW	11	12,328,342 (GRCm39)	missense	probably damaging	1.00
R3407:Cobl	UTSW	11	12,325,830 (GRCm39)	missense	probably damaging	1.00
R4627:Cobl	UTSW	11	12,201,093 (GRCm39)	missense	probably damaging	1.00
R4662:Cobl	UTSW	11	12,203,672 (GRCm39)	missense	probably benign	0.08
R4677:Cobl	UTSW	11	12,336,665 (GRCm39)	missense	possibly damaging	0.93
R4844:Cobl	UTSW	11	12,204,740 (GRCm39)	missense	probably benign	0.10
R4942:Cobl	UTSW	11	12,204,185 (GRCm39)	missense	probably damaging	0.99
R5158:Cobl	UTSW	11	12,206,198 (GRCm39)	missense	possibly damaging	0.84
R5195:Cobl	UTSW	11	12,203,565 (GRCm39)	missense	probably benign	0.02
R5255:Cobl	UTSW	11	12,325,825 (GRCm39)	missense	probably damaging	1.00
R5588:Cobl	UTSW	11	12,293,886 (GRCm39)	nonsense	probably null
R5637:Cobl	UTSW	11	12,246,531 (GRCm39)	intron	probably benign
R5749:Cobl	UTSW	11	12,216,965 (GRCm39)	missense	possibly damaging	0.86
R5953:Cobl	UTSW	11	12,206,220 (GRCm39)	missense	probably benign	0.00
R6000:Cobl	UTSW	11	12,319,684 (GRCm39)	missense	probably benign	0.08
R6373:Cobl	UTSW	11	12,203,118 (GRCm39)	missense	probably damaging	1.00
R7034:Cobl	UTSW	11	12,204,177 (GRCm39)	missense	probably damaging	1.00
R7071:Cobl	UTSW	11	12,204,795 (GRCm39)	missense	probably benign	0.00
R7077:Cobl	UTSW	11	12,203,441 (GRCm39)	missense	probably benign	0.04
R7078:Cobl	UTSW	11	12,328,271 (GRCm39)	missense	probably damaging	1.00
R7099:Cobl	UTSW	11	12,246,540 (GRCm39)	missense
R7153:Cobl	UTSW	11	12,204,128 (GRCm39)	missense	probably damaging	1.00
R7448:Cobl	UTSW	11	12,206,225 (GRCm39)	missense	possibly damaging	0.46
R7519:Cobl	UTSW	11	12,203,124 (GRCm39)	missense	probably damaging	1.00
R7767:Cobl	UTSW	11	12,362,117 (GRCm39)	start gained	probably benign
R7772:Cobl	UTSW	11	12,204,488 (GRCm39)	missense	probably benign	0.29
R7841:Cobl	UTSW	11	12,203,324 (GRCm39)	missense	probably damaging	1.00
R7845:Cobl	UTSW	11	12,315,139 (GRCm39)	missense	probably benign	0.35
R8026:Cobl	UTSW	11	12,203,459 (GRCm39)	missense	probably benign	0.01
R8118:Cobl	UTSW	11	12,204,834 (GRCm39)	missense	probably benign	0.03
R8192:Cobl	UTSW	11	12,199,745 (GRCm39)	missense	probably benign	0.07
R8320:Cobl	UTSW	11	12,217,001 (GRCm39)	missense	probably damaging	1.00
R8338:Cobl	UTSW	11	12,203,696 (GRCm39)	missense	probably benign	0.41
R9319:Cobl	UTSW	11	12,203,648 (GRCm39)	missense	probably benign	0.00
R9497:Cobl	UTSW	11	12,203,501 (GRCm39)	missense	probably benign	0.00
R9501:Cobl	UTSW	11	12,328,235 (GRCm39)	missense	possibly damaging	0.94
Z1176:Cobl	UTSW	11	12,325,827 (GRCm39)	missense	probably damaging	1.00
Z1176:Cobl	UTSW	11	12,319,645 (GRCm39)	missense	probably damaging	1.00
Z1176:Cobl	UTSW	11	12,203,433 (GRCm39)	missense	probably benign	0.02

Predicted Primers

PCR Primer
(F):5'- GCTCAAGTCTAACGGCAGTAGG -3'
(R):5'- GACGTCAGGTGCCACAAAAG -3'

Sequencing Primer
(F):5'- TCTAACGGCAGTAGGAACCACTG -3'
(R):5'- GGTGCCACAAAAGCCTCC -3'

Posted On

2016-11-08