Mutagenetix > Incidental Mutation

Incidental Mutation 'R5646:Vmn2r76'

441153

Institutional Source

Beutler Lab

Gene Symbol

Vmn2r76

Ensembl Gene

ENSMUSG00000091239

Gene Name

vomeronasal 2, receptor 76

Synonyms

MMRRC Submission

043294-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.166) question?

Stock #

R5646 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

85874414-85895409 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

C to A at 85875261 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Cysteine to Phenylalanine at position 572 (C572F)

Ref Sequence

ENSEMBL: ENSMUSP00000127309 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000165771]

AlphaFold

E9Q3F5

Predicted Effect

probably damaging
Transcript: ENSMUST00000165771
AA Change: C572F

PolyPhen 2 Score 0.975 (Sensitivity: 0.76; Specificity: 0.96)

SMART Domains

Protein: ENSMUSP00000127309
Gene: ENSMUSG00000091239
AA Change: C572F

Domain	Start	End	E-Value	Type
signal peptide	1	21	N/A	INTRINSIC
Pfam:ANF_receptor	78	470	1.2e-29	PFAM
low complexity region	476	489	N/A	INTRINSIC
Pfam:NCD3G	513	565	3.7e-22	PFAM
Pfam:7tm_3	598	833	1.4e-52	PFAM

Coding Region Coverage

1x: 99.3%
3x: 98.7%
10x: 97.5%
20x: 95.8%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 75 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Add1	C	A	5: 34,788,024 (GRCm39)	A691D	probably benign	Het
Agap3	C	A	5: 24,688,395 (GRCm39)	D394E	probably benign	Het
Arhgef11	T	C	3: 87,591,793 (GRCm39)	V59A	possibly damaging	Het
Atp2a1	A	G	7: 126,052,277 (GRCm39)	V402A	probably benign	Het
Bbs9	T	C	9: 22,490,011 (GRCm39)	F261L	probably benign	Het
Bsn	A	T	9: 107,987,631 (GRCm39)		probably benign	Het
Camk1	A	G	6: 113,316,301 (GRCm39)	V81A	probably damaging	Het
Cass4	T	G	2: 172,258,165 (GRCm39)	C54W	probably damaging	Het
Ccdc39	A	T	3: 33,879,699 (GRCm39)	F456L	probably damaging	Het
Cdc42bpa	A	G	1: 179,933,659 (GRCm39)	E766G	probably damaging	Het
Cdcp2	T	C	4: 106,962,339 (GRCm39)	C171R	probably damaging	Het
Cdh9	G	A	15: 16,823,371 (GRCm39)	E118K	probably damaging	Het
Col4a2	G	A	8: 11,491,281 (GRCm39)	G1227R	probably damaging	Het
Cyp2d9	A	G	15: 82,336,665 (GRCm39)	T5A	probably benign	Het
Dlg5	T	A	14: 24,208,767 (GRCm39)	D813V	probably damaging	Het
Dmtf1	G	A	5: 9,174,515 (GRCm39)	S405F	possibly damaging	Het
Drd2	A	G	9: 49,316,212 (GRCm39)	K324R	probably benign	Het
Ergic1	T	C	17: 26,833,332 (GRCm39)	S29P	probably damaging	Het
Fmnl1	A	G	11: 103,087,338 (GRCm39)		probably benign	Het
Foxd2	C	T	4: 114,765,832 (GRCm39)	A63T	unknown	Het
Gfap	T	C	11: 102,782,282 (GRCm39)	I409M	probably benign	Het
Gfpt1	A	G	6: 87,019,981 (GRCm39)	M1V	probably null	Het
H2-T23	C	G	17: 36,342,695 (GRCm39)	E148Q	possibly damaging	Het
Hip1	G	A	5: 135,457,595 (GRCm39)	R704W	probably damaging	Het
Kif19a	A	C	11: 114,670,480 (GRCm39)	D130A	probably damaging	Het
Lin7b	T	C	7: 45,018,617 (GRCm39)	D14G	probably damaging	Het
Meioc	T	A	11: 102,566,083 (GRCm39)	N510K	possibly damaging	Het
Mettl5	C	A	2: 69,711,663 (GRCm39)	G68*	probably null	Het
Mitf	A	T	6: 97,990,655 (GRCm39)	I184F	probably damaging	Het
Mmp9	T	A	2: 164,790,970 (GRCm39)	H119Q	probably benign	Het
Mycbp2	T	A	14: 103,407,346 (GRCm39)	H2768L	probably benign	Het
Myh2	A	T	11: 67,079,638 (GRCm39)	I1032F	probably benign	Het
Myo3b	T	C	2: 70,144,774 (GRCm39)	I1110T	probably damaging	Het
Nde1	T	C	16: 13,987,378 (GRCm39)	F8L	probably damaging	Het
Or10aa3	C	T	1: 173,878,853 (GRCm39)	Q305*	probably null	Het
Or2a25	A	G	6: 42,888,457 (GRCm39)		probably null	Het
Or2h2b-ps1	T	C	17: 37,480,808 (GRCm39)	I244V	probably benign	Het
Or4f47	T	C	2: 111,973,028 (GRCm39)	V246A	possibly damaging	Het
Or51r1	A	C	7: 102,228,512 (GRCm39)	Y270S	possibly damaging	Het
Or5ac20	C	T	16: 59,104,342 (GRCm39)	V173I	probably benign	Het
Or6c202	T	C	10: 128,996,706 (GRCm39)	D49G	possibly damaging	Het
Pcdhga8	A	G	18: 37,859,823 (GRCm39)	H293R	probably benign	Het
Pde7a	A	T	3: 19,287,937 (GRCm39)	L244Q	probably damaging	Het
Pex14	T	C	4: 149,045,910 (GRCm39)	D340G	probably benign	Het
Pfkfb4	A	T	9: 108,837,489 (GRCm39)	I195F	probably damaging	Het
Pik3c2a	C	A	7: 116,005,186 (GRCm39)	G361W	probably damaging	Het
Prkcg	T	A	7: 3,377,597 (GRCm39)	M517K	probably damaging	Het
Prob1	A	T	18: 35,787,167 (GRCm39)	F362L	probably benign	Het
Rims4	C	T	2: 163,705,937 (GRCm39)	W232*	probably null	Het
Robo2	T	C	16: 73,758,707 (GRCm39)	D688G	probably damaging	Het
Sec24c	C	A	14: 20,729,641 (GRCm39)	A73E	probably benign	Het
Slc15a3	A	G	19: 10,820,574 (GRCm39)	N64D	probably benign	Het
Smg1	A	T	7: 117,811,782 (GRCm39)	N65K	probably benign	Het
Spata31h1	A	G	10: 82,119,610 (GRCm39)	S4467P	probably damaging	Het
Sptb	G	T	12: 76,634,215 (GRCm39)	D2165E	probably benign	Het
Sptbn5	A	T	2: 119,879,292 (GRCm39)		noncoding transcript	Het
Syt12	A	T	19: 4,506,569 (GRCm39)	M192K	possibly damaging	Het
Tas2r107	A	G	6: 131,636,671 (GRCm39)	V126A	probably benign	Het
Thbs3	T	C	3: 89,126,405 (GRCm39)	L242P	probably damaging	Het
Timp2	A	T	11: 118,208,358 (GRCm39)		probably null	Het
Tmtc1	G	A	6: 148,148,329 (GRCm39)	A751V	probably damaging	Het
Tnni2	A	T	7: 141,997,650 (GRCm39)	D85V	probably damaging	Het
Ttc39b	G	A	4: 83,162,307 (GRCm39)	P319L	probably damaging	Het
Ttc6	A	T	12: 57,622,805 (GRCm39)	K68M	probably damaging	Het
Usp25	G	A	16: 76,847,360 (GRCm39)	R156Q	probably benign	Het
Vill	A	T	9: 118,900,230 (GRCm39)	D365V	probably damaging	Het
Vmn1r65	T	A	7: 6,012,223 (GRCm39)	T4S	probably benign	Het
Vps26a	A	C	10: 62,304,077 (GRCm39)	N181K	probably damaging	Het
Xirp2	T	C	2: 67,341,134 (GRCm39)	L1125P	probably damaging	Het
Zbtb41	T	G	1: 139,351,501 (GRCm39)	S205A	probably benign	Het
Zfp438	T	C	18: 5,214,526 (GRCm39)	D144G	probably benign	Het
Zfp600	T	C	4: 146,131,670 (GRCm39)	S113P	probably damaging	Het
Zfp84	C	T	7: 29,475,818 (GRCm39)	T170M	probably benign	Het
Zmiz2	T	A	11: 6,352,837 (GRCm39)	D654E	probably damaging	Het
Zswim4	A	T	8: 84,957,739 (GRCm39)		probably null	Het

Other mutations in Vmn2r76

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00969:Vmn2r76	APN	7	85,877,925 (GRCm39)	missense	probably benign
IGL01374:Vmn2r76	APN	7	85,874,857 (GRCm39)	missense	probably benign	0.02
IGL01419:Vmn2r76	APN	7	85,874,910 (GRCm39)	missense	probably benign	0.32
IGL01627:Vmn2r76	APN	7	85,874,871 (GRCm39)	missense	probably damaging	1.00
IGL01730:Vmn2r76	APN	7	85,879,406 (GRCm39)	missense	probably benign	0.02
IGL01957:Vmn2r76	APN	7	85,877,925 (GRCm39)	missense	probably benign
IGL02214:Vmn2r76	APN	7	85,879,138 (GRCm39)	missense	probably benign	0.07
IGL02489:Vmn2r76	APN	7	85,878,071 (GRCm39)	missense	probably benign	0.41
IGL02543:Vmn2r76	APN	7	85,879,356 (GRCm39)	missense	probably benign	0.06
IGL02579:Vmn2r76	APN	7	85,877,961 (GRCm39)	nonsense	probably null
IGL02598:Vmn2r76	APN	7	85,877,879 (GRCm39)	missense	probably benign	0.05
IGL02720:Vmn2r76	APN	7	85,874,914 (GRCm39)	missense	probably benign	0.35
IGL02745:Vmn2r76	APN	7	85,879,495 (GRCm39)	missense	probably benign	0.06
IGL03393:Vmn2r76	APN	7	85,879,034 (GRCm39)	missense	probably benign	0.01
R0483:Vmn2r76	UTSW	7	85,874,959 (GRCm39)	missense	probably damaging	1.00
R0513:Vmn2r76	UTSW	7	85,877,987 (GRCm39)	missense	probably benign	0.01
R0528:Vmn2r76	UTSW	7	85,879,506 (GRCm39)	missense	possibly damaging	0.80
R0601:Vmn2r76	UTSW	7	85,875,323 (GRCm39)	critical splice acceptor site	probably null
R0662:Vmn2r76	UTSW	7	85,879,578 (GRCm39)	missense	probably benign	0.39
R0883:Vmn2r76	UTSW	7	85,877,904 (GRCm39)	missense	probably benign	0.00
R1532:Vmn2r76	UTSW	7	85,879,454 (GRCm39)	missense	probably benign	0.02
R1694:Vmn2r76	UTSW	7	85,879,356 (GRCm39)	missense	probably benign	0.06
R1696:Vmn2r76	UTSW	7	85,880,464 (GRCm39)	missense	possibly damaging	0.56
R2135:Vmn2r76	UTSW	7	85,880,219 (GRCm39)	missense	probably benign	0.02
R2151:Vmn2r76	UTSW	7	85,879,692 (GRCm39)	missense	probably benign
R2181:Vmn2r76	UTSW	7	85,874,743 (GRCm39)	missense	probably benign	0.00
R2268:Vmn2r76	UTSW	7	85,879,707 (GRCm39)	missense	probably benign	0.03
R2877:Vmn2r76	UTSW	7	85,875,201 (GRCm39)	missense	probably benign	0.00
R3155:Vmn2r76	UTSW	7	85,874,959 (GRCm39)	missense	probably damaging	1.00
R3746:Vmn2r76	UTSW	7	85,874,763 (GRCm39)	missense	probably benign	0.11
R3799:Vmn2r76	UTSW	7	85,875,244 (GRCm39)	missense	probably benign	0.00
R3825:Vmn2r76	UTSW	7	85,880,415 (GRCm39)	missense	probably benign	0.10
R4058:Vmn2r76	UTSW	7	85,879,508 (GRCm39)	missense	probably benign	0.00
R4237:Vmn2r76	UTSW	7	85,879,740 (GRCm39)	missense	probably benign	0.00
R4404:Vmn2r76	UTSW	7	85,877,511 (GRCm39)	missense	probably benign	0.16
R4796:Vmn2r76	UTSW	7	85,879,652 (GRCm39)	missense	possibly damaging	0.95
R4838:Vmn2r76	UTSW	7	85,874,733 (GRCm39)	missense	probably damaging	1.00
R5175:Vmn2r76	UTSW	7	85,877,915 (GRCm39)	missense	probably benign	0.00
R5268:Vmn2r76	UTSW	7	85,875,267 (GRCm39)	missense	probably damaging	1.00
R5381:Vmn2r76	UTSW	7	85,874,496 (GRCm39)	missense	probably damaging	1.00
R5531:Vmn2r76	UTSW	7	85,874,657 (GRCm39)	missense	probably damaging	1.00
R5566:Vmn2r76	UTSW	7	85,875,286 (GRCm39)	missense	probably damaging	1.00
R5664:Vmn2r76	UTSW	7	85,895,202 (GRCm39)	critical splice donor site	probably null
R5818:Vmn2r76	UTSW	7	85,879,142 (GRCm39)	missense	probably benign	0.00
R6093:Vmn2r76	UTSW	7	85,877,469 (GRCm39)	nonsense	probably null
R6651:Vmn2r76	UTSW	7	85,878,059 (GRCm39)	missense	possibly damaging	0.64
R6741:Vmn2r76	UTSW	7	85,879,560 (GRCm39)	missense	probably benign
R6750:Vmn2r76	UTSW	7	85,875,114 (GRCm39)	missense	probably damaging	1.00
R7082:Vmn2r76	UTSW	7	85,874,440 (GRCm39)	missense	probably benign	0.01
R7136:Vmn2r76	UTSW	7	85,877,975 (GRCm39)	missense	probably benign	0.06
R7524:Vmn2r76	UTSW	7	85,879,374 (GRCm39)	missense	probably benign	0.00
R7524:Vmn2r76	UTSW	7	85,874,577 (GRCm39)	missense	probably benign	0.22
R7611:Vmn2r76	UTSW	7	85,879,388 (GRCm39)	missense	probably benign	0.04
R7833:Vmn2r76	UTSW	7	85,877,892 (GRCm39)	missense	probably benign
R8002:Vmn2r76	UTSW	7	85,879,271 (GRCm39)	missense	probably benign	0.05
R8021:Vmn2r76	UTSW	7	85,874,958 (GRCm39)	missense	probably damaging	1.00
R8023:Vmn2r76	UTSW	7	85,879,028 (GRCm39)	missense	probably benign	0.00
R8250:Vmn2r76	UTSW	7	85,875,231 (GRCm39)	missense	possibly damaging	0.82
R8428:Vmn2r76	UTSW	7	85,874,479 (GRCm39)	missense	possibly damaging	0.63
R8874:Vmn2r76	UTSW	7	85,877,999 (GRCm39)	missense	probably damaging	1.00
R9139:Vmn2r76	UTSW	7	85,879,170 (GRCm39)	missense	probably benign	0.02
R9357:Vmn2r76	UTSW	7	85,880,428 (GRCm39)	missense	probably benign	0.43
Z1176:Vmn2r76	UTSW	7	85,895,271 (GRCm39)	missense	probably benign	0.39

Predicted Primers

PCR Primer
(F):5'- GTGGTTGTCCAATGAATAGCAGAG -3'
(R):5'- GCTTCCATAAGAAAGGTCAATTTGG -3'

Sequencing Primer
(F):5'- TGTCCAATGAATAGCAGAGAACAAAG -3'
(R):5'- TTCCTGGTGTATAGATAAATCTT -3'

Posted On

2016-11-08